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Yorodumi- EMDB-53976: Rabbit 80S ribosome in complex with eRF1-AAQ, stalled at the Stop... -
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Open data
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Basic information
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| Title | Rabbit 80S ribosome in complex with eRF1-AAQ, stalled at the Stop codon in mutated F2A sequence | |||||||||||||||
Map data | Sharpened, masked map (using B-factor of -10 and provided mask) | |||||||||||||||
Sample |
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Keywords | Translation / Recoding / Nascent peptide / Foot-and-mouth disease virus / F2A / StopGo / RIBOSOME | |||||||||||||||
| Function / homology | Function and homology information[histone H4]-N-methyl-L-lysine20 N-methyltransferase / regulation of macromolecule metabolic process / L-peptidase / [histone H4]-lysine20 N-methyltransferase / translation termination factor activity / histone H4K20 methyltransferase activity / translation release factor complex / cytoplasmic translational termination / symbiont-mediated perturbation of host chromatin organization / regulation of translational termination ...[histone H4]-N-methyl-L-lysine20 N-methyltransferase / regulation of macromolecule metabolic process / L-peptidase / [histone H4]-lysine20 N-methyltransferase / translation termination factor activity / histone H4K20 methyltransferase activity / translation release factor complex / cytoplasmic translational termination / symbiont-mediated perturbation of host chromatin organization / regulation of translational termination / protein methylation / translation release factor activity, codon specific / ribosomal subunit / translation release factor activity / S-adenosyl-L-methionine binding / sequence-specific mRNA binding / peptidyl-tRNA hydrolase activity / nuclear-transcribed mRNA catabolic process, nonsense-mediated decay / positive regulation of double-strand break repair via nonhomologous end joining / laminin receptor activity / Protein hydroxylation / Eukaryotic Translation Termination / ubiquitin ligase inhibitor activity / positive regulation of signal transduction by p53 class mediator / 90S preribosome / Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC) / pericentric heterochromatin / translational termination / phagocytic cup / protein-RNA complex assembly / Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC) / laminin binding / rough endoplasmic reticulum / translation regulator activity / ribosomal small subunit export from nucleus / gastrulation / MDM2/MDM4 family protein binding / cytosolic ribosome / class I DNA-(apurinic or apyrimidinic site) endonuclease activity / DNA-(apurinic or apyrimidinic site) lyase / ribosomal large subunit biogenesis / maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / positive regulation of apoptotic signaling pathway / maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / picornain 3C / maturation of SSU-rRNA / small-subunit processome / T=pseudo3 icosahedral viral capsid / ribonucleoside triphosphate phosphatase activity / host cell cytoplasmic vesicle membrane / spindle / Regulation of expression of SLITs and ROBOs / cytoplasmic ribonucleoprotein granule / rRNA processing / antimicrobial humoral immune response mediated by antimicrobial peptide / positive regulation of canonical Wnt signaling pathway / rhythmic process / heparin binding / regulation of translation / nucleoside-triphosphate phosphatase / large ribosomal subunit / channel activity / ribosome binding / virus receptor activity / ribosomal small subunit biogenesis / ribosomal small subunit assembly / 5S rRNA binding / ribosomal large subunit assembly / small ribosomal subunit / small ribosomal subunit rRNA binding / large ribosomal subunit rRNA binding / cytosolic small ribosomal subunit / monoatomic ion transmembrane transport / methylation / defense response to Gram-negative bacterium / perikaryon / clathrin-dependent endocytosis of virus by host cell / cytosolic large ribosomal subunit / killing of cells of another organism / cytoplasmic translation / cell differentiation / tRNA binding / RNA helicase activity / negative regulation of translation / mitochondrial inner membrane / postsynaptic density / rRNA binding / viral protein processing / structural constituent of ribosome / host cell endoplasmic reticulum membrane / ribosome / translation / ribonucleoprotein complex / cell division / RNA-directed RNA polymerase / cysteine-type endopeptidase activity / viral RNA genome replication / DNA repair / RNA-directed RNA polymerase activity / mRNA binding Similarity search - Function | |||||||||||||||
| Biological species | ![]() Foot-and-mouth disease virus SAT 2 / Homo sapiens (human) | |||||||||||||||
| Method | single particle reconstruction / cryo EM / Resolution: 2.65 Å | |||||||||||||||
Authors | Li X / Zuber PK / Loughran G / Bhatt PR / Alquraish F / Ramakrishnan V / Firth AE / Atkins JF | |||||||||||||||
| Funding support | United Kingdom, European Union, 4 items
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Citation | Journal: To Be PublishedTitle: Molecular architecture and diversity of StopGo/2A translational recoding Authors: Li X / Zuber PK / Loughran G / Bhatt PR / Alquraish F / Ramakrishnan V / Firth AE / Atkins JF | |||||||||||||||
| History |
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Structure visualization
| Supplemental images |
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Downloads & links
-EMDB archive
| Map data | emd_53976.map.gz | 155.9 MB | EMDB map data format | |
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| Header (meta data) | emd-53976-v30.xml emd-53976.xml | 149 KB 149 KB | Display Display | EMDB header |
| FSC (resolution estimation) | emd_53976_fsc.xml | 21.2 KB | Display | FSC data file |
| Images | emd_53976.png | 158.1 KB | ||
| Masks | emd_53976_msk_1.map | 824 MB | Mask map | |
| Filedesc metadata | emd-53976.cif.gz | 25.4 KB | ||
| Others | emd_53976_additional_1.map.gz emd_53976_additional_2.map.gz emd_53976_half_map_1.map.gz emd_53976_half_map_2.map.gz | 664.2 MB 469.7 MB 668.7 MB 668.7 MB | ||
| Archive directory | http://ftp.pdbj.org/pub/emdb/structures/EMD-53976 ftp://ftp.pdbj.org/pub/emdb/structures/EMD-53976 | HTTPS FTP |
-Validation report
| Summary document | emd_53976_validation.pdf.gz | 1.1 MB | Display | EMDB validaton report |
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| Full document | emd_53976_full_validation.pdf.gz | 1.1 MB | Display | |
| Data in XML | emd_53976_validation.xml.gz | 29.3 KB | Display | |
| Data in CIF | emd_53976_validation.cif.gz | 39.1 KB | Display | |
| Arichive directory | https://ftp.pdbj.org/pub/emdb/validation_reports/EMD-53976 ftp://ftp.pdbj.org/pub/emdb/validation_reports/EMD-53976 | HTTPS FTP |
-Related structure data
| Related structure data | ![]() 9rhuMC M: atomic model generated by this map C: citing same article ( |
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| Similar structure data | Similarity search - Function & homology F&H Search |
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Links
| EMDB pages | EMDB (EBI/PDBe) / EMDataResource |
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| Related items in Molecule of the Month |
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Map
| File | Download / File: emd_53976.map.gz / Format: CCP4 / Size: 824 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) | ||||||||||||||||||||||||||||||||||||
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| Annotation | Sharpened, masked map (using B-factor of -10 and provided mask) | ||||||||||||||||||||||||||||||||||||
| Projections & slices | Image control
Images are generated by Spider. | ||||||||||||||||||||||||||||||||||||
| Voxel size | X=Y=Z: 0.702 Å | ||||||||||||||||||||||||||||||||||||
| Density |
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| Symmetry | Space group: 1 | ||||||||||||||||||||||||||||||||||||
| Details | EMDB XML:
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-Supplemental data
-Mask #1
| File | emd_53976_msk_1.map | ||||||||||||
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| Density Histograms |
-Additional map: Unsharpened map from 3D refinement
| File | emd_53976_additional_1.map | ||||||||||||
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| Annotation | Unsharpened map from 3D refinement | ||||||||||||
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| Density Histograms |
-Additional map: Map sharpened according to local resolution
| File | emd_53976_additional_2.map | ||||||||||||
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| Annotation | Map sharpened according to local resolution | ||||||||||||
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| Density Histograms |
-Half map: Half map 1
| File | emd_53976_half_map_1.map | ||||||||||||
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| Annotation | Half map 1 | ||||||||||||
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| Density Histograms |
-Half map: Half map 2
| File | emd_53976_half_map_2.map | ||||||||||||
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| Annotation | Half map 2 | ||||||||||||
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| Density Histograms |
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Sample components
+Entire : Rabbit 80S ribosome stalled at the stop codon of a PGX mutant of ...
+Supramolecule #1: Rabbit 80S ribosome stalled at the stop codon of a PGX mutant of ...
+Supramolecule #2: 40S subunit
+Supramolecule #3: 60S subunit
+Supramolecule #4: P- and E-site tRNAs and nascent polypeptide chain
+Supramolecule #5: 40S subunit proteins
+Supramolecule #6: 18S rRNA
+Supramolecule #7: 60 subunit proteins
+Supramolecule #8: 5S, 5.8S, 28S rRNAs
+Macromolecule #1: 60S ribosomal protein L34
+Macromolecule #3: 60S ribosomal protein L6
+Macromolecule #5: Small ribosomal subunit protein eS27
+Macromolecule #6: 60S ribosomal protein L7
+Macromolecule #8: Small ribosomal subunit protein eS28
+Macromolecule #9: Large ribosomal subunit protein eL8
+Macromolecule #11: Ubiquitin-ribosomal protein eS31 fusion protein
+Macromolecule #12: 60S ribosomal protein L9
+Macromolecule #13: Large ribosomal subunit protein uL2
+Macromolecule #14: 40S ribosomal protein eS30
+Macromolecule #15: 60S ribosomal protein L10
+Macromolecule #16: Ribosomal protein L3
+Macromolecule #17: eS26
+Macromolecule #18: 60S ribosomal protein L11
+Macromolecule #19: Large ribosomal subunit protein uL4
+Macromolecule #20: Small ribosomal subunit protein RACK1
+Macromolecule #21: Choriogonadotropin subunit beta variant 1,Genome polyprotein,Geno...
+Macromolecule #22: Large ribosomal subunit protein uL18
+Macromolecule #23: Small ribosomal subunit protein uS14
+Macromolecule #24: Large ribosomal subunit protein eL13
+Macromolecule #26: 60S ribosomal protein L14
+Macromolecule #28: Ribosomal protein L15
+Macromolecule #30: Large ribosomal subunit protein uL13
+Macromolecule #31: Small ribosomal subunit protein uS2
+Macromolecule #32: Large ribosomal subunit protein uL22
+Macromolecule #33: 40S ribosomal protein S3a
+Macromolecule #34: eL18
+Macromolecule #35: 40S ribosomal protein S2
+Macromolecule #36: Ribosomal protein L19
+Macromolecule #37: Small ribosomal subunit protein uS3
+Macromolecule #38: Large ribosomal subunit protein eL20
+Macromolecule #39: 40S ribosomal protein S4
+Macromolecule #40: 60S ribosomal protein L21
+Macromolecule #41: Small ribosomal subunit protein uS7
+Macromolecule #42: 60S ribosomal protein L22
+Macromolecule #43: 40S ribosomal protein S6
+Macromolecule #44: Ribosomal protein L23
+Macromolecule #45: Small ribosomal subunit protein eS7
+Macromolecule #46: Ribosomal protein L24
+Macromolecule #47: 40S ribosomal protein S8
+Macromolecule #48: Large ribosomal subunit protein uL23
+Macromolecule #49: Small ribosomal subunit protein uS4
+Macromolecule #50: Ribosomal protein L26
+Macromolecule #51: eS10
+Macromolecule #52: 60S ribosomal protein L27
+Macromolecule #53: Small ribosomal subunit protein uS17
+Macromolecule #54: 60S ribosomal protein L27a
+Macromolecule #55: Small ribosomal subunit protein eS12
+Macromolecule #56: 60S ribosomal protein L29
+Macromolecule #57: Small ribosomal subunit protein uS15
+Macromolecule #58: 60S ribosomal protein L30
+Macromolecule #59: Small ribosomal subunit protein uS11
+Macromolecule #60: 60S ribosomal protein L31
+Macromolecule #61: Small ribosomal subunit protein uS19
+Macromolecule #62: Ribosomal protein L32
+Macromolecule #63: Small ribosomal subunit protein uS9
+Macromolecule #64: 60S ribosomal protein L35a
+Macromolecule #65: Small ribosomal subunit protein eS17
+Macromolecule #66: Small ribosomal subunit protein uS13
+Macromolecule #67: 60S ribosomal protein L35
+Macromolecule #68: 40S ribosomal protein eS19
+Macromolecule #69: Large ribosomal subunit protein eL36
+Macromolecule #70: Small ribosomal subunit protein uS10
+Macromolecule #71: Ribosomal protein L37
+Macromolecule #72: Small ribosomal subunit protein eS21
+Macromolecule #73: 60S ribosomal protein L38
+Macromolecule #74: Small ribosomal subunit protein uS8
+Macromolecule #75: 60S ribosomal protein L39-like
+Macromolecule #76: 40S ribosomal protein S23
+Macromolecule #77: Ubiquitin-ribosomal protein eL40 fusion protein
+Macromolecule #78: 40S ribosomal protein S24
+Macromolecule #79: Ribosomal protein L36a
+Macromolecule #80: Small ribosomal subunit protein eS25
+Macromolecule #81: 60S ribosomal protein L37a
+Macromolecule #82: Small ribosomal subunit protein eS32
+Macromolecule #83: [histone H4]-N-methyl-L-lysine20 N-methyltransferase KMT5B
+Macromolecule #84: Large ribosomal subunit protein uL10
+Macromolecule #85: 60S ribosomal protein L12
+Macromolecule #86: Ribosomal protein uL1
+Macromolecule #87: Eukaryotic peptide chain release factor subunit 1
+Macromolecule #88: ATP binding cassette subfamily E member 1
+Macromolecule #2: 18S rRNA
+Macromolecule #4: 28S rRNA
+Macromolecule #7: 5S rRNA
+Macromolecule #10: 5.8S rRNA
+Macromolecule #25: mRNA containing F2A sequence
+Macromolecule #27: E-site tRNA-Pro
+Macromolecule #29: P-site tRNA-Gly
+Macromolecule #89: MAGNESIUM ION
+Macromolecule #90: ZINC ION
+Macromolecule #91: POTASSIUM ION
+Macromolecule #92: SPERMIDINE
+Macromolecule #93: GUANOSINE-5'-TRIPHOSPHATE
+Macromolecule #94: IRON/SULFUR CLUSTER
-Experimental details
-Structure determination
| Method | cryo EM |
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Processing | single particle reconstruction |
| Aggregation state | particle |
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Sample preparation
| Concentration | 0.4 mg/mL | |||||||||||||||
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| Buffer | pH: 7.4 Component:
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| Grid | Model: Quantifoil R1.2/1.3 / Material: GOLD / Mesh: 300 / Support film - Material: GRAPHENE OXIDE / Pretreatment - Type: GLOW DISCHARGE / Pretreatment - Time: 45 sec. / Pretreatment - Atmosphere: AIR | |||||||||||||||
| Vitrification | Cryogen name: ETHANE / Chamber humidity: 100 % / Chamber temperature: 277 K / Instrument: FEI VITROBOT MARK II |
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Electron microscopy
| Microscope | TFS KRIOS |
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| Image recording | Film or detector model: GATAN K3 (6k x 4k) / Number grids imaged: 2 / Number real images: 22565 / Average exposure time: 1.2 sec. / Average electron dose: 40.0 e/Å2 |
| Electron beam | Acceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN |
| Electron optics | C2 aperture diameter: 50.0 µm / Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Cs: 2.7 mm / Nominal defocus max: 2.2 µm / Nominal defocus min: 1.0 µm / Nominal magnification: 105000 |
| Sample stage | Specimen holder model: FEI TITAN KRIOS AUTOGRID HOLDER / Cooling holder cryogen: NITROGEN |
| Experimental equipment | ![]() Model: Titan Krios / Image courtesy: FEI Company |
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About Yorodumi



Keywords
Foot-and-mouth disease virus SAT 2
Homo sapiens (human)
Authors
United Kingdom, European Union, 4 items
Citation


























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Processing
FIELD EMISSION GUN



