ジャーナル: J Struct Biol / 年: 2013 タイトル: Membrane curvature in flaviviruses. 著者: Wei Zhang / Bärbel Kaufmann / Paul R Chipman / Richard J Kuhn / Michael G Rossmann / 要旨: Coordinated interplay between membrane proteins and the lipid bilayer is required for such processes as transporter function and the entrance of enveloped viruses into host cells. In this study, ...Coordinated interplay between membrane proteins and the lipid bilayer is required for such processes as transporter function and the entrance of enveloped viruses into host cells. In this study, three-dimensional cryo-electron microscopy density maps of mature and immature flaviviruses were analyzed to assess the curvature of the membrane leaflets and its relation to membrane-bound viral glycoproteins. The overall morphology of the viral membrane is determined by the icosahedral scaffold composed of envelope (E) and membrane (M) proteins through interaction of the proteins' stem-anchor regions with the membrane. In localized regions, small membrane areas exhibit convex, concave, flat or saddle-shaped surfaces that are constrained by the specific protein organization within each membrane leaflet. These results suggest that the organization of membrane proteins in small enveloped viruses mediate the formation of membrane curvature.
ダウンロード / ファイル: emd_5296.map.gz / 形式: CCP4 / 大きさ: 61.8 MB / タイプ: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
注釈
This is a reconstruction map of West Nile virus
ボクセルのサイズ
X=Y=Z: 2.95 Å
密度
表面レベル
登録者による: 40.0 / ムービー #1: 40
最小 - 最大
-217.592849730000012 - 558.695617680000055
平均 (標準偏差)
10.370124819999999 (±78.856483460000007)
対称性
空間群: 1
詳細
EMDB XML:
マップ形状
Axis order
X
Y
Z
Origin
-127
-107
-127
サイズ
255
255
255
Spacing
255
255
255
セル
A=B=C: 752.25 Å α=β=γ: 90.0 °
CCP4マップ ヘッダ情報:
mode
Image stored as Reals
Å/pix. X/Y/Z
2.95
2.95
2.95
M x/y/z
255
255
255
origin x/y/z
0.000
0.000
0.000
length x/y/z
752.250
752.250
752.250
α/β/γ
90.000
90.000
90.000
start NX/NY/NZ
-62
-62
-62
NX/NY/NZ
125
125
125
MAP C/R/S
1
2
3
start NC/NR/NS
-107
-127
-127
NC/NR/NS
255
255
255
D min/max/mean
-217.593
558.696
10.370
-
添付データ
-
試料の構成要素
-
全体 : West Nile Virus
全体
名称: West Nile Virus (西ナイルウイルス)
要素
試料: West Nile Virus
ウイルス: West Nile virus (西ナイルウイルス)
-
超分子 #1000: West Nile Virus
超分子
名称: West Nile Virus / タイプ: sample / ID: 1000 / Number unique components: 3
-
超分子 #1: West Nile virus
超分子
名称: West Nile virus / タイプ: virus / ID: 1 / Name.synonym: West Nile Virus / NCBI-ID: 11082 / 生物種: West Nile virus / データベース: NCBI / ウイルスタイプ: VIRION / ウイルス・単離状態: STRAIN / ウイルス・エンベロープ: Yes / ウイルス・中空状態: No / Syn species name: West Nile Virus
宿主
生物種: Homo sapiens (ヒト) / 別称: VERTEBRATES
ウイルス殻
Shell ID: 1 / 名称: E / 直径: 500 Å / T番号(三角分割数): 1
-
実験情報
-
構造解析
手法
ネガティブ染色法, クライオ電子顕微鏡法
解析
単粒子再構成法
試料の集合状態
particle
-
試料調製
緩衝液
pH: 8 / 詳細: 12 mM Tris-HCl,120 mM NaCl, 1 mM EDTA
染色
タイプ: NEGATIVE / 詳細: none
グリッド
詳細: 400 mesh holey carbon grid
凍結
凍結剤: ETHANE / 装置: OTHER / 詳細: vitrification carried out in a BSL3 lab