Journal: J Struct Biol / Year: 2013 Title: Membrane curvature in flaviviruses. Authors: Wei Zhang / Bärbel Kaufmann / Paul R Chipman / Richard J Kuhn / Michael G Rossmann / Abstract: Coordinated interplay between membrane proteins and the lipid bilayer is required for such processes as transporter function and the entrance of enveloped viruses into host cells. In this study, ...Coordinated interplay between membrane proteins and the lipid bilayer is required for such processes as transporter function and the entrance of enveloped viruses into host cells. In this study, three-dimensional cryo-electron microscopy density maps of mature and immature flaviviruses were analyzed to assess the curvature of the membrane leaflets and its relation to membrane-bound viral glycoproteins. The overall morphology of the viral membrane is determined by the icosahedral scaffold composed of envelope (E) and membrane (M) proteins through interaction of the proteins' stem-anchor regions with the membrane. In localized regions, small membrane areas exhibit convex, concave, flat or saddle-shaped surfaces that are constrained by the specific protein organization within each membrane leaflet. These results suggest that the organization of membrane proteins in small enveloped viruses mediate the formation of membrane curvature.
History
Deposition
May 25, 2011
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Header (metadata) release
Oct 26, 2011
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Map release
Sep 19, 2012
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Update
Jul 24, 2013
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Current status
Jul 24, 2013
Processing site: RCSB / Status: Released
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Structure visualization
Movie
Surface view with section colored by density value
Name: West Nile Virus / type: sample / ID: 1000 / Number unique components: 3
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Supramolecule #1: West Nile virus
Supramolecule
Name: West Nile virus / type: virus / ID: 1 / Name.synonym: West Nile Virus / NCBI-ID: 11082 / Sci species name: West Nile virus / Database: NCBI / Virus type: VIRION / Virus isolate: STRAIN / Virus enveloped: Yes / Virus empty: No / Syn species name: West Nile Virus
Host (natural)
Organism: Homo sapiens (human) / synonym: VERTEBRATES
Virus shell
Shell ID: 1 / Name: E / Diameter: 500 Å / T number (triangulation number): 1
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Experimental details
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Structure determination
Method
negative staining, cryo EM
Processing
single particle reconstruction
Aggregation state
particle
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Sample preparation
Buffer
pH: 8 / Details: 12 mM Tris-HCl,120 mM NaCl, 1 mM EDTA
Staining
Type: NEGATIVE / Details: none
Grid
Details: 400 mesh holey carbon grid
Vitrification
Cryogen name: ETHANE / Instrument: OTHER / Details: vitrification carried out in a BSL3 lab
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Electron microscopy
Microscope
FEI/PHILIPS CM300FEG/T
Image recording
Category: FILM / Film or detector model: KODAK SO-163 FILM / Digitization - Scanner: ZEISS SCAI / Digitization - Sampling interval: 7 µm / Number real images: 83 / Average electron dose: 30 e/Å2
Electron beam
Acceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
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