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- EMDB-51173: Composite map of MtUvrA2UvrB2-DNA -

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Basic information

Entry
Database: EMDB / ID: EMD-51173
TitleComposite map of MtUvrA2UvrB2-DNA
Map data
Sample
  • Complex: MtUvrA2UvrB2-DNA complex
    • Complex: MtUvrA2UvrB2
      • Protein or peptide: UvrABC system protein A
      • Protein or peptide: UvrABC system protein B
    • Complex: DNA
      • DNA: DNA
  • Ligand: ZINC ION
KeywordsDNA repair / NER / UVRA / UVRB / UVR / MTB / DNA BINDING PROTEIN
Function / homology
Function and homology information


negative regulation of strand invasion / excinuclease ABC activity / excinuclease repair complex / symbiont-mediated perturbation of host innate immune response / response to nitrosative stress / SOS response / peptidoglycan-based cell wall / nucleotide-excision repair / DNA damage response / ATP hydrolysis activity ...negative regulation of strand invasion / excinuclease ABC activity / excinuclease repair complex / symbiont-mediated perturbation of host innate immune response / response to nitrosative stress / SOS response / peptidoglycan-based cell wall / nucleotide-excision repair / DNA damage response / ATP hydrolysis activity / DNA binding / zinc ion binding / ATP binding / plasma membrane / cytosol / cytoplasm
Similarity search - Function
UvrA, interaction domain / UvrA interaction domain / UvrABC system subunit A / UvrA DNA-binding domain / UvrA DNA-binding domain / UvrB, YAD/RRR-motif-containing domain / Ultra-violet resistance protein B / UvrABC system, subunit B / UVR domain superfamily / UvrB/uvrC motif ...UvrA, interaction domain / UvrA interaction domain / UvrABC system subunit A / UvrA DNA-binding domain / UvrA DNA-binding domain / UvrB, YAD/RRR-motif-containing domain / Ultra-violet resistance protein B / UvrABC system, subunit B / UVR domain superfamily / UvrB/uvrC motif / UvrB, interaction domain / UvrB interaction domain / UVR domain / UVR domain profile. / Helicase/UvrB, N-terminal / Type III restriction enzyme, res subunit / ABC transporter-like, conserved site / ABC transporters family signature. / Helicase conserved C-terminal domain / ABC transporter-like, ATP-binding domain / ATP-binding cassette, ABC transporter-type domain profile. / helicase superfamily c-terminal domain / Superfamilies 1 and 2 helicase C-terminal domain profile. / Superfamilies 1 and 2 helicase ATP-binding type-1 domain profile. / DEAD-like helicases superfamily / Helicase, C-terminal / Helicase superfamily 1/2, ATP-binding domain / P-loop containing nucleoside triphosphate hydrolase
Similarity search - Domain/homology
UvrABC system protein B / UvrABC system protein A
Similarity search - Component
Biological speciesMycobacterium tuberculosis (bacteria) / Escherichia coli (E. coli)
Methodsingle particle reconstruction / cryo EM / Resolution: 3.7 Å
AuthorsGenta M / Capelli R / Ferrara G / Rizzi M / Rossi F / Jeruzalmi D / Bolognesi M / Chaves-Sanjuan A / Miggiano R
Funding support Italy, 1 items
OrganizationGrant numberCountry
Ministero dell Universita e della RicercaP2022P8KMF Italy
CitationJournal: To Be Published
Title: Composite MtUvrA2UvrB2-DNA structure
Authors: Genta M / Capelli R / Ferrara G / Rizzi M / Rossi F / Jeruzalmi D / Bolognesi M / Chaves-Sanjuan A / Miggiano R
History
DepositionJul 26, 2024-
Header (metadata) releaseApr 23, 2025-
Map releaseApr 23, 2025-
UpdateApr 23, 2025-
Current statusApr 23, 2025Processing site: PDBe / Status: Released

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Structure visualization

Supplemental images

emd_51173.png

Downloads & links

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Map

FileDownload / File: emd_51173.map.gz / Format: CCP4 / Size: 244.1 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Projections & slices

Image control

Size
Brightness
Contrast
Others
AxesZ (Sec.)Y (Row.)X (Col.)
0.84 Å/pix.
x 400 pix.
= 336. Å		0.84 Å/pix.
x 400 pix.
= 336. Å		0.84 Å/pix.
x 400 pix.
= 336. Å

Surface

Projections

Slices (1/3)

Slices (1/2)

Slices (2/3)

Images are generated by Spider.

Voxel sizeX=Y=Z: 0.84 Å
Density

Histogram

Histogram (log scale)
Contour LevelBy AUTHOR: 0.22
Minimum - Maximum-0.39526108 - 0.93026894
Average (Standard dev.)0.0029184353 (±0.03561288)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions400400400
Spacing400400400
CellA=B=C: 336.0 Å
α=β=γ: 90.0 °

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Supplemental data

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Sample components

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Entire : MtUvrA2UvrB2-DNA complex

EntireName: MtUvrA2UvrB2-DNA complex
Components
  • Complex: MtUvrA2UvrB2-DNA complex
    • Complex: MtUvrA2UvrB2
      • Protein or peptide: UvrABC system protein A
      • Protein or peptide: UvrABC system protein B
    • Complex: DNA
      • DNA: DNA
  • Ligand: ZINC ION

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Supramolecule #1: MtUvrA2UvrB2-DNA complex

SupramoleculeName: MtUvrA2UvrB2-DNA complex / type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1-#3 / Details: Heterotetrameric MtUvrA2UvrB2 in complex with DNA
Molecular weightTheoretical: 400 KDa

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Supramolecule #2: MtUvrA2UvrB2

SupramoleculeName: MtUvrA2UvrB2 / type: complex / ID: 2 / Parent: 1 / Macromolecule list: #1-#2
Source (natural)Organism: Mycobacterium tuberculosis (bacteria)

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Supramolecule #3: DNA

SupramoleculeName: DNA / type: complex / ID: 3 / Parent: 1 / Macromolecule list: #3
Source (natural)Organism: Escherichia coli (E. coli)

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Macromolecule #1: UvrABC system protein A

MacromoleculeName: UvrABC system protein A / type: protein_or_peptide / ID: 1 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Mycobacterium tuberculosis (bacteria)
Molecular weightTheoretical: 108.806398 KDa
Recombinant expressionOrganism: Escherichia coli (E. coli)
SequenceString: MGHHHHHHHH HHSSGHIEGR HMADRLIVKG AREHNLRSVD LDLPRDALIV FTGLSGSGKS SLAFDTIFAE GQRRYVESLS AYARQFLGQ MDKPDVDFIE GLSPAVSIDQ KSTNRNPRST VGTITEVYDY LRLLYARAGT PHCPTCGERV ARQTPQQIVD Q VLAMPEGT ...String:
MGHHHHHHHH HHSSGHIEGR HMADRLIVKG AREHNLRSVD LDLPRDALIV FTGLSGSGKS SLAFDTIFAE GQRRYVESLS AYARQFLGQ MDKPDVDFIE GLSPAVSIDQ KSTNRNPRST VGTITEVYDY LRLLYARAGT PHCPTCGERV ARQTPQQIVD Q VLAMPEGT RFLVLAPVVR TRKGEFADLF DKLNAQGYSR VRVDGVVHPL TDPPKLKKQE KHDIEVVVDR LTVKAAAKRR LT DSVETAL NLADGIVVLE FVDHELGAPH REQRFSEKLA CPNGHALAVD DLEPRSFSFN SPYGACPECS GLGIRKEVDP ELV VPDPDR TLAQGAVAPW SNGHTAEYFT RMMAGLGEAL GFDVDTPWRK LPAKARKAIL EGADEQVHVR YRNRYGRTRS YYAD FEGVL AFLQRKMSQT ESEQMKERYE GFMRDVPCPV CAGTRLKPEI LAVTLAGESK GEHGAKSIAE VCELSIADCA DFLNA LTLG PREQAIAGQV LKEIRSRLGF LLDVGLEYLS LSRAAATLSG GEAQRIRLAT QIGSGLVGVL YVLDEPSIGL HQRDNR RLI ETLTRLRDLG NTLIVVEHDE DTIEHADWIV DIGPGAGEHG GRIVHSGPYD ELLRNKDSIT GAYLSGRESI EIPAIRR SV DPRRQLTVVG AREHNLRGID VSFPLGVLTS VTGVSGSGKS TLVNDILAAV LANRLNGARQ VPGRHTRVTG LDYLDKLV R VDQSPIGRTP RSNPATYTGV FDKIRTLFAA TTEAKVRGYQ PGRFSFNVKG GRCEACTGDG TIKIEMNFLP DVYVPCEVC QGARYNRETL EVHYKGKTVS EVLDMSIEEA AEFFEPIAGV HRYLRTLVDV GLGYVRLGQP APTLSGGEAQ RVKLASELQK RSTGRTVYI LDEPTTGLHF DDIRKLLNVI NGLVDKGNTV IVIEHNLDVI KTSDWIIDLG PEGGAGGGTV VAQGTPEDVA A VPASYTGK FLAEVVGGGA SAATSRSNRR RNVSA

UniProtKB: UvrABC system protein A

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Macromolecule #2: UvrABC system protein B

MacromoleculeName: UvrABC system protein B / type: protein_or_peptide / ID: 2 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Mycobacterium tuberculosis (bacteria)
Molecular weightTheoretical: 80.800156 KDa
Recombinant expressionOrganism: Escherichia coli (E. coli)
SequenceString: MGHHHHHHHH HHSSGHIEGR HMVRAGGHFE VVSPHAPAGD QPAAIDELER RINAGERDVV LLGATGTGKS ATTAWLIERL QRPTLVMAP NKTLAAQLAN ELREMLPHNA VEYFVSYYDY YQPEAYIAQT DTYIEKDSSI NDDVERLRHS ATSALLSRRD V VVVASVSC ...String:
MGHHHHHHHH HHSSGHIEGR HMVRAGGHFE VVSPHAPAGD QPAAIDELER RINAGERDVV LLGATGTGKS ATTAWLIERL QRPTLVMAP NKTLAAQLAN ELREMLPHNA VEYFVSYYDY YQPEAYIAQT DTYIEKDSSI NDDVERLRHS ATSALLSRRD V VVVASVSC IYGLGTPQSY LDRSVELKVG EEVPRDGLLR LLVDVQYTRN DMSFTRGSFR VRGDTVEIIP SYEELAVRIE FF GDEIEAL YYLHPLTGEV IRQVDSLRIF PATHYVAGPE RMAHAVSAIE EELAERLAEL ESQGKLLEAQ RLRMRTNYDI EMM RQVGFC SGIENYSRHI DGRGPGTPPA TLLDYFPEDF LLVIDESHVT VPQIGGMYEG DISRKRNLVE YGFRLPSACD NRPL TWEEF ADRIGQTVYL SATPGPYELS QTGGEFVEQV IRPTGLVDPK VVVKPTKGQI DDLIGEIRTR ADADQRVLVT TLTKK MAED LTDYLLEMGI RVRYLHSEVD TLRRVELLRQ LRLGDYDVLV GINLLREGLD LPEVSLVAIL DADKEGFLRS SRSLIQ TIG RAARNVSGEV HMYADKITDS MREAIDETER RRAKQIAYNE ANGIDPQPLR KKIADILDQV YREADDTAVV EVGGSGR NA SRGRRAQGEP GRAVSAGVFE GRDTSAMPRA ELADLIKDLT AQMMAAARDL QFELAARFRD EIADLKRELR GMDAAGLK

UniProtKB: UvrABC system protein B

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Macromolecule #3: DNA

MacromoleculeName: DNA / type: dna / ID: 3 / Number of copies: 2 / Classification: DNA
Source (natural)Organism: Escherichia coli (E. coli)
Molecular weightTheoretical: 12.89029 KDa
SequenceString:
(DT)(DA)(DG)(DT)(DC)(DA)(DC)(DA)(DT)(DC) (DA)(DG)(DT)(DG)(DA)(DT)(DC)(DA)(DG)(DT) (DG)(DG)(DT)(DT)(DC)(DC)(DG)(DG)(DA) (DA)(DC)(DC)(DA)(DC)(DT)(DG)(DA)(DT)(DC) (DA) (DC)(DT)

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Macromolecule #4: ZINC ION

MacromoleculeName: ZINC ION / type: ligand / ID: 4 / Number of copies: 4 / Formula: ZN
Molecular weightTheoretical: 65.409 Da

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

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Sample preparation

Concentration0.5 mg/mL
BufferpH: 8
Component:
ConcentrationFormulaName
300.0 mMNaClNaCl
20.0 mMTris HCl
GridModel: Quantifoil R1.2/1.3 / Material: COPPER / Mesh: 300 / Support film - Material: CARBON / Support film - topology: HOLEY / Pretreatment - Type: GLOW DISCHARGE / Pretreatment - Time: 30 sec. / Pretreatment - Atmosphere: AIR / Details: 30mA
VitrificationCryogen name: ETHANE / Chamber humidity: 100 % / Chamber temperature: 277 K / Instrument: FEI VITROBOT MARK IV

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Electron microscopy

MicroscopeFEI TALOS ARCTICA
Image recordingFilm or detector model: GATAN K3 (6k x 4k) / Number grids imaged: 1 / Number real images: 21232 / Average electron dose: 40.0 e/Å2
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Cs: 2.7 mm / Nominal defocus max: 2.5 µm / Nominal defocus min: 0.8 µm / Nominal magnification: 105000
Sample stageSpecimen holder model: FEI TITAN KRIOS AUTOGRID HOLDER / Cooling holder cryogen: NITROGEN
Experimental equipment
Model: Talos Arctica / Image courtesy: FEI Company

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Image processing

Particle selectionNumber selected: 666671
Startup modelType of model: INSILICO MODEL
Final reconstructionApplied symmetry - Point group: C1 (asymmetric) / Resolution.type: BY AUTHOR / Resolution: 3.7 Å / Resolution method: FSC 0.143 CUT-OFF / Software - Name: cryoSPARC / Details: The final map is a composite map of two. / Number images used: 110916
Initial angle assignmentType: MAXIMUM LIKELIHOOD / Software - Name: cryoSPARC
Final angle assignmentType: MAXIMUM LIKELIHOOD / Software - Name: cryoSPARC
Final 3D classificationSoftware - Name: cryoSPARC

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