EMDB-50856: Structure of CliM-stalled Bacillus subtilis 70S ribosome with emp...

Basic information

Entry

Database: EMDB / ID: EMD-50856

• Title: Structure of CliM-stalled Bacillus subtilis 70S ribosome with empty A-site

Sample

• Complex: Structure of CliM-stalled Bacillus subtilis 70S ribosome with empty A-site
  • Complex: Bacillus subtilis 70S ribosome
    • Protein or peptide: x 46 types
    • RNA: x 4 types
  • Complex: Nascent Chain CliM
    • Protein or peptide: x 1 types
  • Complex: mRNA
    • RNA: x 1 types

• Keywords: Arrest peptide / Stalled ribosome complex / Bacillus subtilis / ApcF / RIBOSOME

Function / homology

Function:

positive regulation of rRNA processing / nucleoid / rRNA processing / large ribosomal subunit / transferase activity / ribosome biogenesis / ribosomal small subunit biogenesis / 5S rRNA binding / small ribosomal subunit / ribosomal large subunit assembly / small ribosomal subunit rRNA binding / cytosolic small ribosomal subunit / large ribosomal subunit rRNA binding / cytosolic large ribosomal subunit / cytoplasmic translation / tRNA binding / negative regulation of translation / rRNA binding / structural constituent of ribosome / ribosome / translation / ribonucleoprotein complex / response to antibiotic / mRNA binding / DNA binding / RNA binding / zinc ion binding / metal ion binding / cytoplasm / cytosol

Domain/homology:

: / Ribosomal protein S14, type Z / Ribosomal protein L31 type A / Ribosomal protein S16, conserved site / Ribosomal protein S16 signature. / Ribosomal protein L31 signature. / Ribosomal protein L31 / Ribosomal protein L31 superfamily / Ribosomal protein L31 / Ribosomal protein L16 signature 1. / Ribosomal protein L6, conserved site / Ribosomal protein L6 signature 1. / Ribosomal protein L21, conserved site / : / Ribosomal protein L21 signature. / Ribosomal protein L16 signature 2. / Ribosomal protein L16, conserved site / Ribosomal protein L17 signature. / Ribosomal protein L36 signature. / : / Ribosomal protein L28/L24 superfamily / Ribosomal protein L33, conserved site / Ribosomal protein L33 signature. / Ribosomal protein L32p, bacterial type / Ribosomal protein L35, conserved site / Ribosomal protein L35 signature. / Ribosomal protein L28 / Ribosomal protein L35, non-mitochondrial / Ribosomal protein L18, bacterial-type / Ribosomal protein S6, conserved site / Ribosomal protein S6 signature. / Ribosomal protein S3, bacterial-type / Ribosomal protein S13, bacterial-type / Ribosomal protein S19, bacterial-type / : / Ribosomal protein L6, bacterial-type / Ribosomal protein S7, bacterial/organellar-type / Ribosomal protein S11, bacterial-type / Ribosomal protein S20 / Ribosomal protein S20 superfamily / Ribosomal protein S20 / Ribosomal protein L5, bacterial-type / Ribosomal protein S4, bacterial-type / Ribosomal protein S5, bacterial-type / Ribosomal protein L19, conserved site / Ribosomal protein L19 signature. / 30S ribosomal protein S17 / : / Ribosomal protein S6, plastid/chloroplast / Ribosomal protein L36 / Ribosomal protein L36 superfamily / Ribosomal protein L36 / Ribosomal protein L20 signature. / Ribosomal protein S14/S29 / Ribosomal protein L34, conserved site / Ribosomal protein L34 signature. / Ribosomal protein L14P, bacterial-type / Ribosomal protein L27, conserved site / Ribosomal protein L27 signature. / Ribosomal protein S2, bacteria/mitochondria/plastid / Ribosomal protein L35 / Ribosomal protein L35 superfamily / Ribosomal protein L22, bacterial/chloroplast-type / Ribosomal protein L35 / Ribosomal protein L2, bacterial/organellar-type / Ribosomal protein L33 / Ribosomal protein L18 / Ribosomal L18 of archaea, bacteria, mitoch. and chloroplast / Ribosomal protein S18, conserved site / Ribosomal protein S18 signature. / Ribosomal protein L33 / Ribosomal L28 family / Ribosomal protein S9, bacterial/plastid / Ribosomal protein L33 superfamily / Ribosomal protein L28/L24 / Ribosomal protein L30, bacterial-type / Ribosomal protein S16 / Ribosomal protein S16 domain superfamily / Ribosomal protein S16 / L28p-like / Ribosomal protein L16 / Ribosomal protein S15, bacterial-type / Ribosomal protein S6 / Ribosomal protein S6 / Ribosomal protein S6 superfamily / Ribosomal protein L20 / Ribosomal protein S12, bacterial-type / Ribosomal protein L20 / Ribosomal protein L20, C-terminal / Ribosomal protein L19 / Ribosomal protein L19 / Ribosomal protein L19 superfamily / : / Large ribosomal subunit protein uL24, C-terminal domain / Translation elongation factor EF1B/ribosomal protein S6 / Ribosomal protein L17 / Ribosomal protein L17 superfamily / Ribosomal protein L17 / Ribosomal protein L27 / Ribosomal L27 protein

Component:

Uncharacterized protein / Large ribosomal subunit protein bL19 / Large ribosomal subunit protein bL32 / Small ribosomal subunit protein uS11 / Large ribosomal subunit protein bL34 / Large ribosomal subunit protein bL27 / Large ribosomal subunit protein uL24 / Large ribosomal subunit protein uL29 / Small ribosomal subunit protein uS17 / Large ribosomal subunit protein uL14 / Large ribosomal subunit protein uL5 / Small ribosomal subunit protein uS14B / Small ribosomal subunit protein uS8 / Large ribosomal subunit protein uL16 / Large ribosomal subunit protein uL15 / Large ribosomal subunit protein uL30 / Large ribosomal subunit protein bL17 / Large ribosomal subunit protein bL36 / Small ribosomal subunit protein uS13 / Small ribosomal subunit protein uS2 / Small ribosomal subunit protein uS3 / Small ribosomal subunit protein uS4 / Small ribosomal subunit protein uS5 / Small ribosomal subunit protein bS6 / Small ribosomal subunit protein uS7 / Small ribosomal subunit protein uS9 / Small ribosomal subunit protein uS10 / Small ribosomal subunit protein uS12 / Small ribosomal subunit protein uS15 / Small ribosomal subunit protein bS16 / Small ribosomal subunit protein bS18 / Small ribosomal subunit protein uS19 / Small ribosomal subunit protein bS20 / Large ribosomal subunit protein bL21 / Large ribosomal subunit protein bL28 / Large ribosomal subunit protein uL22 / Large ribosomal subunit protein uL2 / Large ribosomal subunit protein uL3 / Large ribosomal subunit protein uL4 / Large ribosomal subunit protein uL23 / Large ribosomal subunit protein uL6 / Large ribosomal subunit protein uL18 / Large ribosomal subunit protein bL20 / Large ribosomal subunit protein bL35 / Large ribosomal subunit protein bL33A / Large ribosomal subunit protein uL13 / Large ribosomal subunit protein bL31

• Biological species: Bacillus subtilis subsp. subtilis str. 168 (bacteria) / Clostridioides difficile 630 (bacteria)
• Method: single particle reconstruction / cryo EM / Resolution: 2.3 Å
• Authors: Gersteuer F / Wilson DN

Funding support

Germany, 1 items

Organization	Grant number	Country
German Research Foundation (DFG)		Germany

• Citation: Journal: Nat Commun / Year: 2026; Title: Diverse mechanisms of translation arrest by a Clostridia ribosome stalling peptide CliM.; Authors: Mayu Yoshida / Felix Gersteuer / Ole Berendes / Keigo Fujiwara / Haaris A Safdari / Helge Paternoga / Hiraku Takada / Nozomu Obana / Helmut Grubmüller / Lars V Bock / Daniel N Wilson / Shinobu Chiba / ; Abstract: Ribosome arrest peptides undergo programmed translational stalling in response to changes in the cellular environment to feedback-regulate gene expression. CliM, an arrest peptide in Clostridia, is encoded upstream of the YidC membrane protein insertase gene, but its function and mechanism remain unclear. Here we show that CliM monitors YidC activity to maintain adequate cellular YidC capacity. Interestingly, Clostridium kluyveri CliM induces elongation arrest at multiple sense codons, whereas Clostridioides difficile CliM causes termination arrest. Cryo-EM-based structural and mutational analyses demonstrate that C. difficile CliM adopts multiple α-helices within the nascent polypeptide exit tunnel, where it forms extensive arrest-essential interactions with the ribosome. The residue immediately N-terminal to the stalling site contributes to arrest by sterically interfering with full accommodation of the release factor or aminoacyl-tRNA in the A-site. Molecular dynamics simulations suggest that membrane insertion of CliM induces sequential unwinding of these α-helical structures and relocation of the penultimate residue, thereby triggering arrest release. These findings provide a unified mechanistic framework that explains the distinct arrest behaviors of CliM homologs.

History

Deposition	Jul 2, 2024	-
Header (metadata) release	Jan 14, 2026	-
Map release	Jan 14, 2026	-
Update	May 27, 2026	-
Current status	May 27, 2026	Processing site: PDBe / Status: Released

Map

• File: Download / File: emd_50856.map.gz / Format: CCP4 / Size: 216 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
• Voxel size: X=Y=Z: 0.831 Å

Density

Contour Level	By AUTHOR: 0.008
Minimum - Maximum	-0.016166804 - 0.060315996
Average (Standard dev.)	0.00048272754 (±0.0028812673)

• Symmetry: Space group: 1

Details

EMDB XML:

Map geometry	Axis order X Y Z Origin 0 0 0 Dimensions 384 384 384 Spacing 384 384 384
Cell	A=B=C: 319.104 Å α=β=γ: 90.0 °

Supplemental data

Mask #1

• File: emd_50856_msk_1.map

Additional map: #1

• File: emd_50856_additional_1.map

Half map: #1

• File: emd_50856_half_map_1.map

Half map: #2

• File: emd_50856_half_map_2.map

Sample components

Entire : Structure of CliM-stalled Bacillus subtilis 70S ribosome with emp...

• Entire: Name: Structure of CliM-stalled Bacillus subtilis 70S ribosome with empty A-site

Components

• Complex: Structure of CliM-stalled Bacillus subtilis 70S ribosome with empty A-site
  • Complex: Bacillus subtilis 70S ribosome
    • Protein or peptide: Large ribosomal subunit protein bL32
    • Protein or peptide: 50S ribosomal protein L33 1
    • Protein or peptide: 50S ribosomal protein L34
    • Protein or peptide: 50S ribosomal protein L35
    • Protein or peptide: 50S ribosomal protein L36
    • Protein or peptide: 50S ribosomal protein L31
    • RNA: 5S rRNA
    • Protein or peptide: Large ribosomal subunit protein uL2
    • Protein or peptide: 50S ribosomal protein L3
    • Protein or peptide: 50S ribosomal protein L4
    • Protein or peptide: 50S ribosomal protein L5
    • Protein or peptide: Large ribosomal subunit protein uL6
    • Protein or peptide: 50S ribosomal protein L13
    • Protein or peptide: 50S ribosomal protein L14
    • Protein or peptide: 50S ribosomal protein L15
    • Protein or peptide: 50S ribosomal protein L16
    • Protein or peptide: 50S ribosomal protein L17
    • Protein or peptide: 50S ribosomal protein L18
    • Protein or peptide: 50S ribosomal protein L19
    • Protein or peptide: Large ribosomal subunit protein bL20
    • Protein or peptide: 50S ribosomal protein L21
    • Protein or peptide: 50S ribosomal protein L22
    • Protein or peptide: Large ribosomal subunit protein uL23
    • Protein or peptide: 50S ribosomal protein L24
    • Protein or peptide: Large ribosomal subunit protein bL27
    • Protein or peptide: Large ribosomal subunit protein bL28
    • Protein or peptide: 50S ribosomal protein L29
    • Protein or peptide: Large ribosomal subunit protein uL30
    • Protein or peptide: Small ribosomal subunit protein uS3
    • Protein or peptide: 30S ribosomal protein S5
    • Protein or peptide: 30S ribosomal protein S6
    • Protein or peptide: Small ribosomal subunit protein uS7
    • Protein or peptide: 30S ribosomal protein S8
    • Protein or peptide: Small ribosomal subunit protein uS9
    • Protein or peptide: 30S ribosomal protein S10
    • Protein or peptide: 30S ribosomal protein S11
    • Protein or peptide: Small ribosomal subunit protein uS12
    • Protein or peptide: Small ribosomal subunit protein uS13
    • Protein or peptide: 30S ribosomal protein S14
    • Protein or peptide: 30S ribosomal protein S15
    • Protein or peptide: 30S ribosomal protein S16
    • Protein or peptide: 30S ribosomal protein S17
    • Protein or peptide: 30S ribosomal protein S18
    • Protein or peptide: 30S ribosomal protein S20
    • RNA: P-site tRNA-Phe
    • RNA: 23S rRNA
    • Protein or peptide: 30S ribosomal protein S2
    • Protein or peptide: Small ribosomal subunit protein uS19
    • RNA: 16S rRNA
    • Protein or peptide: 30S ribosomal protein S4
  • Complex: Nascent Chain CliM
    • Protein or peptide: Nascent Chain CliM
  • Complex: mRNA
    • RNA: mRNA

Supramolecule #1: Structure of CliM-stalled Bacillus subtilis 70S ribosome with emp...

• Supramolecule: Name: Structure of CliM-stalled Bacillus subtilis 70S ribosome with empty A-site; type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1-#52

Supramolecule #2: Bacillus subtilis 70S ribosome

• Supramolecule: Name: Bacillus subtilis 70S ribosome / type: complex / ID: 2 / Parent: 1 / Macromolecule list: #1-#48, #51-#52
• Source (natural): Organism: Bacillus subtilis subsp. subtilis str. 168 (bacteria)

Supramolecule #3: Nascent Chain CliM

• Supramolecule: Name: Nascent Chain CliM / type: complex / ID: 3 / Parent: 1 / Macromolecule list: #49
• Source (natural): Organism: Clostridioides difficile 630 (bacteria)

Supramolecule #4: mRNA

• Supramolecule: Name: mRNA / type: complex / ID: 4 / Parent: 1 / Macromolecule list: #50
• Source (natural): Organism: Clostridioides difficile 630 (bacteria)

Macromolecule #1: Large ribosomal subunit protein bL32

• Macromolecule: Name: Large ribosomal subunit protein bL32 / type: protein_or_peptide / ID: 1 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Bacillus subtilis subsp. subtilis str. 168 (bacteria)
• Molecular weight: Theoretical: 6.713008 KDa

Sequence

String:

MAVPFRRTSK VKKRLRRTHF KLNVPGMTEC PSCGEMKLSH RVCKACGSYN GKDINVKSN

UniProtKB: Large ribosomal subunit protein bL32

Macromolecule #2: 50S ribosomal protein L33 1

• Macromolecule: Name: 50S ribosomal protein L33 1 / type: protein_or_peptide / ID: 2 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Bacillus subtilis subsp. subtilis str. 168 (bacteria)
• Molecular weight: Theoretical: 5.915875 KDa

Sequence

String:

MRVNITLACT ECGERNYISK KNKRNNPDRV EFKKYCPRDK KSTLHRETK

UniProtKB: Large ribosomal subunit protein bL33A

Macromolecule #3: 50S ribosomal protein L34

• Macromolecule: Name: 50S ribosomal protein L34 / type: protein_or_peptide / ID: 3 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Bacillus subtilis subsp. subtilis str. 168 (bacteria)
• Molecular weight: Theoretical: 5.271332 KDa

Sequence

String:

MKRTFQPNNR KRSKVHGFRS RMSSKNGRLV LARRRRKGRK VLSA

UniProtKB: Large ribosomal subunit protein bL34

Macromolecule #4: 50S ribosomal protein L35

• Macromolecule: Name: 50S ribosomal protein L35 / type: protein_or_peptide / ID: 4 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Bacillus subtilis subsp. subtilis str. 168 (bacteria)
• Molecular weight: Theoretical: 7.581128 KDa

Sequence

String:

MPKMKTHRGS AKRFKKTGSG KLKRSHAYTS HLFANKSQKQ KRKLRKSAVV SAGDFKRIKQ MLANIK

UniProtKB: Large ribosomal subunit protein bL35

Macromolecule #5: 50S ribosomal protein L36

• Macromolecule: Name: 50S ribosomal protein L36 / type: protein_or_peptide / ID: 5 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Bacillus subtilis subsp. subtilis str. 168 (bacteria)
• Molecular weight: Theoretical: 4.318422 KDa

Sequence

String:

MKVRPSVKPI CEKCKVIRRK GKVMVICENP KHKQKQG

UniProtKB: Large ribosomal subunit protein bL36

Macromolecule #6: 50S ribosomal protein L31

• Macromolecule: Name: 50S ribosomal protein L31 / type: protein_or_peptide / ID: 6 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Bacillus subtilis subsp. subtilis str. 168 (bacteria)
• Molecular weight: Theoretical: 7.458582 KDa

Sequence

String:

MKAGIHPNFK KATVKCACGN EFETGSVKEE VRVEICSECH PFYTGRQKFA SADGRVDRFN KKYGLK

UniProtKB: Large ribosomal subunit protein bL31

Macromolecule #8: Large ribosomal subunit protein uL2

• Macromolecule: Name: Large ribosomal subunit protein uL2 / type: protein_or_peptide / ID: 8 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Bacillus subtilis subsp. subtilis str. 168 (bacteria)
• Molecular weight: Theoretical: 30.321098 KDa

Sequence

String:

MAIKKYKPTS NGRRGMTTSD FAEITTDKPE KSLLAPLHKK GGRNNQGKLT VRHQGGGHKR QYRVIDFKRD KDGIPGRVAT VEYDPNRSA NIALINYADG EKRYILAPKG IQVGTEIMSG PEADIKVGNA LPLINIPVGT VVHNIELKPG KGGQLVRSAG T SAQVLGKE GKYVLVRLNS GEVRMILSAC RASIGQVGNE QHELINIGKA GRSRWKGVRP TVRGSVMNPN DHPHGGGEGR AP IGRKSPM SPWGKPTLGF KTRKKKNKSD KFIVRRRKNK

UniProtKB: Large ribosomal subunit protein uL2

Macromolecule #9: 50S ribosomal protein L3

• Macromolecule: Name: 50S ribosomal protein L3 / type: protein_or_peptide / ID: 9 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Bacillus subtilis subsp. subtilis str. 168 (bacteria)
• Molecular weight: Theoretical: 22.723348 KDa

Sequence

String:

MTKGILGRKI GMTQVFAENG DLIPVTVIEA APNVVLQKKT AENDGYEAIQ LGFDDKREKL SNKPEKGHVA KAETAPKRFV KELRGVEMD AYEVGQEVKV EIFSAGEIVD VTGVSKGKGF QGAIKRHGQS RGPMSHGSRY HRRPGSMGPV DPNRVFKGKL L PGRMGGEQ ITVQNLEIVK VDAERNLLLI KGNVPGAKKS LITVKSAVKS K

UniProtKB: Large ribosomal subunit protein uL3

Macromolecule #10: 50S ribosomal protein L4

• Macromolecule: Name: 50S ribosomal protein L4 / type: protein_or_peptide / ID: 10 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Bacillus subtilis subsp. subtilis str. 168 (bacteria)
• Molecular weight: Theoretical: 22.424951 KDa

Sequence

String:

MPKVALYNQN GSTAGDIELN ASVFGIEPNE SVVFDAILMQ RASLRQGTHK VKNRSEVRGG GRKPWRQKGT GRARQGSIRS PQWRGGGVV FGPTPRSYSY KLPKKVRRLA IKSVLSSKVI DNNIIVLEDL TLDTAKTKEM AAILKGLSVE KKALIVTADA N EAVALSAR NIPGVTVVEA NGINVLDVVN HEKLLITKAA VEKVEEVLA

UniProtKB: Large ribosomal subunit protein uL4

Macromolecule #11: 50S ribosomal protein L5

• Macromolecule: Name: 50S ribosomal protein L5 / type: protein_or_peptide / ID: 11 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Bacillus subtilis subsp. subtilis str. 168 (bacteria)
• Molecular weight: Theoretical: 20.177564 KDa

Sequence

String:

MNRLKEKYNK EIAPALMTKF NYDSVMQVPK IEKIVINMGV GDAVQNAKAI DSAVEELTFI AGQKPVVTRA KKSIAGFRLR EGMPIGAKV TLRGERMYDF LDKLISVSLP RVRDFRGVSK KSFDGRGNYT LGIKEQLIFP EIDYDKVTKV RGMDIVIVTT A NTDEEARE LLTQVGMPFQ K

UniProtKB: Large ribosomal subunit protein uL5

Macromolecule #12: Large ribosomal subunit protein uL6

• Macromolecule: Name: Large ribosomal subunit protein uL6 / type: protein_or_peptide / ID: 12 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Bacillus subtilis subsp. subtilis str. 168 (bacteria)
• Molecular weight: Theoretical: 19.543389 KDa

Sequence

String:

MSRVGKKLLE IPSDVTVTLN DNNTVAVKGP KGELTRTFHP DMEIKVEDNV LTVARPSDQK EHRALHGTTR SLLGNMVEGV SKGFERGLE LVGVGYRASK SGNKLVLNVG YSHPVEIVPE EGIEIEVPSQ TKVVVKGTDK ERVGAIAANI RAVRSPEPYK G KGIRYEGE VVRRKEGKSA K

UniProtKB: Large ribosomal subunit protein uL6

Macromolecule #13: 50S ribosomal protein L13

• Macromolecule: Name: 50S ribosomal protein L13 / type: protein_or_peptide / ID: 13 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Bacillus subtilis subsp. subtilis str. 168 (bacteria)
• Molecular weight: Theoretical: 16.407104 KDa

Sequence

String:

MRTTPMANAS TIERKWLVVD AAGKTLGRLS SEVAAILRGK HKPTYTPHVD TGDHVIIINA EKIELTGKKL TDKIYYRHTQ HPGGLKSRT ALEMRTNYPE KMLELAIKGM LPKGSLGRQM FKKLNVYRGS EHPHEAQKPE VYELRG

UniProtKB: Large ribosomal subunit protein uL13

Macromolecule #14: 50S ribosomal protein L14

• Macromolecule: Name: 50S ribosomal protein L14 / type: protein_or_peptide / ID: 14 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Bacillus subtilis subsp. subtilis str. 168 (bacteria)
• Molecular weight: Theoretical: 13.175288 KDa

Sequence

String:

MIQQETRLKV ADNSGAREVL TIKVLGGSGR KTANIGDVIV CTVKQATPGG VVKKGEVVKA VIVRTKSGAR RSDGSYISFD ENACVIIRD DKSPRGTRIF GPVARELREN NFMKIVSLAP EVI

UniProtKB: Large ribosomal subunit protein uL14

Macromolecule #15: 50S ribosomal protein L15

• Macromolecule: Name: 50S ribosomal protein L15 / type: protein_or_peptide / ID: 15 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Bacillus subtilis subsp. subtilis str. 168 (bacteria)
• Molecular weight: Theoretical: 15.410694 KDa

Sequence

String:

MKLHELKPSE GSRKTRNRVG RGIGSGNGKT AGKGHKGQNA RSGGGVRPGF EGGQMPLFQR LPKRGFTNIN RKEYAVVNLD KLNGFAEGT EVTPELLLET GVISKLNAGV KILGNGKLEK KLTVKANKFS ASAKEAVEAA GGTAEVI

UniProtKB: Large ribosomal subunit protein uL15

Macromolecule #16: 50S ribosomal protein L16

• Macromolecule: Name: 50S ribosomal protein L16 / type: protein_or_peptide / ID: 16 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Bacillus subtilis subsp. subtilis str. 168 (bacteria)
• Molecular weight: Theoretical: 16.223049 KDa

Sequence

String:

MLLPKRVKYR REHRGKMRGR AKGGTEVHFG EFGIQALEAS WITNRQIEAA RIAMTRYMKR GGKVWIKIFP SKPYTAKPLE VRMGSGKGA PEGWVAVVKP GKVLFEISGV SEEVAREALR LASHKLPIKT KFVKREEIGG ESNES

UniProtKB: Large ribosomal subunit protein uL16

Macromolecule #17: 50S ribosomal protein L17

• Macromolecule: Name: 50S ribosomal protein L17 / type: protein_or_peptide / ID: 17 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Bacillus subtilis subsp. subtilis str. 168 (bacteria)
• Molecular weight: Theoretical: 13.774806 KDa

Sequence

String:

MSYRKLGRTS AQRKAMLRDL TTDLIINERI ETTETRAKEL RSVVEKMITL GKRGDLHARR QAAAYIRNEV ANEENNQDAL QKLFSDIAT RYEERQGGYT RIMKLGPRRG DGAPMAIIEL V

UniProtKB: Large ribosomal subunit protein bL17

Macromolecule #18: 50S ribosomal protein L18

• Macromolecule: Name: 50S ribosomal protein L18 / type: protein_or_peptide / ID: 18 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Bacillus subtilis subsp. subtilis str. 168 (bacteria)
• Molecular weight: Theoretical: 12.993829 KDa

Sequence

String:

MITKTSKNAA RLKRHARVRA KLSGTAERPR LNVFRSNKHI YAQIIDDVNG VTLASASTLD KDLNVESTGD TSAATKVGEL VAKRAAEKG ISDVVFDRGG YLYHGRVKAL ADAAREAGLK F

UniProtKB: Large ribosomal subunit protein uL18

Macromolecule #19: 50S ribosomal protein L19

• Macromolecule: Name: 50S ribosomal protein L19 / type: protein_or_peptide / ID: 19 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Bacillus subtilis subsp. subtilis str. 168 (bacteria)
• Molecular weight: Theoretical: 13.416853 KDa

Sequence

String:

MQKLIEDITK EQLRTDLPAF RPGDTLRVHV KVVEGNRERI QIFEGVVIKR RGGGISETFT VRKISYGVGV ERTFPVHTPK IAKIEVVRY GKVRRAKLYY LRELRGKAAR IKEIRR

UniProtKB: Large ribosomal subunit protein bL19

Macromolecule #20: Large ribosomal subunit protein bL20

• Macromolecule: Name: Large ribosomal subunit protein bL20 / type: protein_or_peptide / ID: 20 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Bacillus subtilis subsp. subtilis str. 168 (bacteria)
• Molecular weight: Theoretical: 13.669189 KDa

Sequence

String:

MPRVKGGTVT RKRRKKVLKL AKGYFGSKHT LYKVANQQVM KSGNYAFRDR RQKKRDFRKL WITRINAAAR MNGLSYSRLM HGLKLSGIE VNRKMLADLA VNDLTAFNQL ADAAKAQLNK

UniProtKB: Large ribosomal subunit protein bL20

Macromolecule #21: 50S ribosomal protein L21

• Macromolecule: Name: 50S ribosomal protein L21 / type: protein_or_peptide / ID: 21 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Bacillus subtilis subsp. subtilis str. 168 (bacteria)
• Molecular weight: Theoretical: 11.296081 KDa

Sequence

String:

MYAIIKTGGK QIKVEEGQTV YIEKLAAEAG ETVTFEDVLF VGGDNVKVGN PTVEGATVTA KVEKQGRAKK ITVFRYKPKK NVHKKQGHR QPYTKVTIEK INA

UniProtKB: Large ribosomal subunit protein bL21

Macromolecule #22: 50S ribosomal protein L22

• Macromolecule: Name: 50S ribosomal protein L22 / type: protein_or_peptide / ID: 22 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Bacillus subtilis subsp. subtilis str. 168 (bacteria)
• Molecular weight: Theoretical: 12.481608 KDa

Sequence

String:

MQAKAVARTV RIAPRKARLV MDLIRGKQVG EAVSILNLTP RAASPIIEKV LKSAIANAEH NYEMDANNLV ISQAFVDEGP TLKRFRPRA MGRASQINKR TSHITIVVSE KKEG

UniProtKB: Large ribosomal subunit protein uL22

Macromolecule #23: Large ribosomal subunit protein uL23

• Macromolecule: Name: Large ribosomal subunit protein uL23 / type: protein_or_peptide / ID: 23 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Bacillus subtilis subsp. subtilis str. 168 (bacteria)
• Molecular weight: Theoretical: 10.978813 KDa

Sequence

String:

MKDPRDVLKR PVITERSADL MTEKKYTFEV DVRANKTEVK DAVESIFGVK VDKVNIMNYK GKSKRVGRYT GMTSRRRKAI VKLTADSKE IEIFEA

UniProtKB: Large ribosomal subunit protein uL23

Macromolecule #24: 50S ribosomal protein L24

• Macromolecule: Name: 50S ribosomal protein L24 / type: protein_or_peptide / ID: 24 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Bacillus subtilis subsp. subtilis str. 168 (bacteria)
• Molecular weight: Theoretical: 11.16612 KDa

Sequence

String:

MHVKKGDKVM VISGKDKGKQ GTILAAFPKK DRVLVEGVNM VKKHSKPTQA NPQGGISNQE APIHVSNVMP LDPKTGEVTR VGYKVEDGK KVRVAKKSGQ VLDK

UniProtKB: Large ribosomal subunit protein uL24

Macromolecule #25: Large ribosomal subunit protein bL27

• Macromolecule: Name: Large ribosomal subunit protein bL27 / type: protein_or_peptide / ID: 25 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Bacillus subtilis subsp. subtilis str. 168 (bacteria)
• Molecular weight: Theoretical: 10.391855 KDa

Sequence

String:

MLRLDLQFFA SKKGVGSTKN GRDSEAKRLG AKRADGQFVT GGSILYRQRG TKIYPGENVG RGGDDTLFAK IDGTVKFERF GRDRKKVSV YPVAQ

UniProtKB: Large ribosomal subunit protein bL27

Macromolecule #26: Large ribosomal subunit protein bL28

• Macromolecule: Name: Large ribosomal subunit protein bL28 / type: protein_or_peptide / ID: 26 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Bacillus subtilis subsp. subtilis str. 168 (bacteria)
• Molecular weight: Theoretical: 6.812117 KDa

Sequence

String:

MARKCVITGK KTTAGNNRSH AMNASKRTWG ANVQKVRILV NGKPKKVYVS ARALKSGKVE RV

UniProtKB: Large ribosomal subunit protein bL28

Macromolecule #27: 50S ribosomal protein L29

• Macromolecule: Name: 50S ribosomal protein L29 / type: protein_or_peptide / ID: 27 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Bacillus subtilis subsp. subtilis str. 168 (bacteria)
• Molecular weight: Theoretical: 7.728029 KDa

Sequence

String:

MKANEIRDLT TAEIEQKVKS LKEELFNLRF QLATGQLENT ARIREVRKAI ARMKTVIRER EIAANK

UniProtKB: Large ribosomal subunit protein uL29

Macromolecule #28: Large ribosomal subunit protein uL30

• Macromolecule: Name: Large ribosomal subunit protein uL30 / type: protein_or_peptide / ID: 28 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Bacillus subtilis subsp. subtilis str. 168 (bacteria)
• Molecular weight: Theoretical: 6.650795 KDa

Sequence

String:

MAKLEITLKR SVIGRPEDQR VTVRTLGLKK TNQTVVHEDN AAIRGMINKV SHLVSVKEQ

UniProtKB: Large ribosomal subunit protein uL30

Macromolecule #29: Small ribosomal subunit protein uS3

• Macromolecule: Name: Small ribosomal subunit protein uS3 / type: protein_or_peptide / ID: 29 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Bacillus subtilis subsp. subtilis str. 168 (bacteria)
• Molecular weight: Theoretical: 24.378914 KDa

Sequence

String:

MGQKVNPVGL RIGVIRDWES KWYAGKDYAD FLHEDLKIRE YISKRLSDAS VSKVEIERAA NRVNITIHTA KPGMVIGKGG SEVEALRKA LNSLTGKRVH INILEIKRAD LDAQLVADNI ARQLENRVSF RRAQKQQIQR TMRAGAQGIK TMVSGRLGGA D IARSEYYS EGTVPLHTLR ADIDYATSEA DTTYGKLGVK VWIYRGEVLP TKKKNEEGGK

UniProtKB: Small ribosomal subunit protein uS3

Macromolecule #30: 30S ribosomal protein S5

• Macromolecule: Name: 30S ribosomal protein S5 / type: protein_or_peptide / ID: 30 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Bacillus subtilis subsp. subtilis str. 168 (bacteria)
• Molecular weight: Theoretical: 17.650625 KDa

Sequence

String:

MRRIDPSKLE LEERLVTVNR VAKVVKGGRR FRFAALVVVG DKNGHVGFGT GKAQEVPEAI RKAVEDAKKN LIEVPMVGTT IPHEIIGRF GAGNILLKPA SEGTGVIAGG PVRAVLELAG VADILSKSLG SNTPINMIRA TLQGLSELKR AEDVAKLRGK S VEELLG

UniProtKB: Small ribosomal subunit protein uS5

Macromolecule #31: 30S ribosomal protein S6

• Macromolecule: Name: 30S ribosomal protein S6 / type: protein_or_peptide / ID: 31 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Bacillus subtilis subsp. subtilis str. 168 (bacteria)
• Molecular weight: Theoretical: 11.140548 KDa

Sequence

String:

MRKYEVMYII RPNIDEESKK AVIERFNNVL TSNGAEITGT KDWGKRRLAY EINDFRDGFY QIVNVQSDAA AVQEFDRLAK ISDDIIRHI VVKEEE

UniProtKB: Small ribosomal subunit protein bS6

Macromolecule #32: Small ribosomal subunit protein uS7

• Macromolecule: Name: Small ribosomal subunit protein uS7 / type: protein_or_peptide / ID: 32 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Bacillus subtilis subsp. subtilis str. 168 (bacteria)
• Molecular weight: Theoretical: 17.915879 KDa

Sequence

String:

MPRKGPVAKR DVLPDPIYNS KLVSRLINKM MIDGKKGKSQ TILYKSFDII KERTGNDAME VFEQALKNIM PVLEVKARRV GGANYQVPV EVRPERRTTL GLRWLVNYAR LRGEKTMEER LANEILDAAN NTGAAVKKRE DTHKMAEANK AFAHYRW

UniProtKB: Small ribosomal subunit protein uS7

Macromolecule #33: 30S ribosomal protein S8

• Macromolecule: Name: 30S ribosomal protein S8 / type: protein_or_peptide / ID: 33 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Bacillus subtilis subsp. subtilis str. 168 (bacteria)
• Molecular weight: Theoretical: 14.901427 KDa

Sequence

String:

MVMTDPIADM LTRIRNANMV RHEKLEIPAS KLKREIAEIL KREGFIRDVE FVEDSKQGII RVFLKYGQNN ERVITGLKRI SKPGLRVYA KSNEVPRVLN GLGIAIISTS QGVLTDKEAR AKQAGGEVLA YVW

UniProtKB: Small ribosomal subunit protein uS8

Macromolecule #34: Small ribosomal subunit protein uS9

• Macromolecule: Name: Small ribosomal subunit protein uS9 / type: protein_or_peptide / ID: 34 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Bacillus subtilis subsp. subtilis str. 168 (bacteria)
• Molecular weight: Theoretical: 14.36952 KDa

Sequence

String:

MAQVQYYGTG RRKSSVARVR LVPGEGRIVV NNREISEHIP SAALIEDIKQ PLTLTETAGT YDVLVNVHGG GFSGQAGAIR HGIARALLE ADPEYRTTLK RAGLLTRDAR MKERKKYGLK GARRAPQFSK R

UniProtKB: Small ribosomal subunit protein uS9

Macromolecule #35: 30S ribosomal protein S10

• Macromolecule: Name: 30S ribosomal protein S10 / type: protein_or_peptide / ID: 35 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Bacillus subtilis subsp. subtilis str. 168 (bacteria)
• Molecular weight: Theoretical: 11.687661 KDa

Sequence

String:

MAKQKIRIRL KAYDHRILDQ SAEKIVETAK RSGASVSGPI PLPTEKSVYT ILRAVHKYKD SREQFEMRTH KRLIDIVNPT PQTVDALMR LDLPSGVDIE IKL

UniProtKB: Small ribosomal subunit protein uS10

Macromolecule #36: 30S ribosomal protein S11

• Macromolecule: Name: 30S ribosomal protein S11 / type: protein_or_peptide / ID: 36 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Bacillus subtilis subsp. subtilis str. 168 (bacteria)
• Molecular weight: Theoretical: 13.952 KDa

Sequence

String:

MAAARKSNTR KRRVKKNIES GIAHIRSTFN NTIVTITDTH GNAISWSSAG ALGFRGSRKS TPFAAQMAAE TAAKGSIEHG LKTLEVTVK GPGSGREAAI RALQAAGLEV TAIRDVTPVP HNGCRPPKRR RV

UniProtKB: Small ribosomal subunit protein uS11

Macromolecule #37: Small ribosomal subunit protein uS12

• Macromolecule: Name: Small ribosomal subunit protein uS12 / type: protein_or_peptide / ID: 37 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Bacillus subtilis subsp. subtilis str. 168 (bacteria)
• Molecular weight: Theoretical: 15.222697 KDa

Sequence

String:

MATINQLIRK GRVSKVENSK SPALNKGYNS FKKEHTNVSS PQKRGVCTRV GTMTPKKPNS ALRKYARVRL TNGIEVTAYI PGIGHNLQE HSVVLIRGGR VKDLPGVRYH IVRGALDTAG VENRAQGRSK YGTKKPKAK

UniProtKB: Small ribosomal subunit protein uS12

Macromolecule #38: Small ribosomal subunit protein uS13

• Macromolecule: Name: Small ribosomal subunit protein uS13 / type: protein_or_peptide / ID: 38 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Bacillus subtilis subsp. subtilis str. 168 (bacteria)
• Molecular weight: Theoretical: 13.832111 KDa

Sequence

String:

MARIAGVDIP RDKRVVISLT YIFGIGRTTA QQILKEAGVS EDTRVRDLTE EELGKIRDII DKLKVEGDLR REVSLNIKRL IEIGSYRGI RHRRGLPVRG QNSKNNARTR KGPRRTVANK KK

UniProtKB: Small ribosomal subunit protein uS13

Macromolecule #39: 30S ribosomal protein S14

• Macromolecule: Name: 30S ribosomal protein S14 / type: protein_or_peptide / ID: 39 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Bacillus subtilis subsp. subtilis str. 168 (bacteria)
• Molecular weight: Theoretical: 7.263803 KDa

Sequence

String:

MAKKSMIAKQ QRTPKFKVQE YTRCERCGRP HSVIRKFKLC RICFRELAYK GQIPGVKKAS W

UniProtKB: Small ribosomal subunit protein uS14B

Macromolecule #40: 30S ribosomal protein S15

• Macromolecule: Name: 30S ribosomal protein S15 / type: protein_or_peptide / ID: 40 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Bacillus subtilis subsp. subtilis str. 168 (bacteria)
• Molecular weight: Theoretical: 10.597224 KDa

Sequence

String:

MAITQERKNQ LINEFKTHES DTGSPEVQIA ILTDSINNLN EHLRTHKKDH HSRRGLLKMV GKRRNLLTYL RNKDVTRYRE LINKLGLRR

UniProtKB: Small ribosomal subunit protein uS15

Macromolecule #41: 30S ribosomal protein S16

• Macromolecule: Name: 30S ribosomal protein S16 / type: protein_or_peptide / ID: 41 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Bacillus subtilis subsp. subtilis str. 168 (bacteria)
• Molecular weight: Theoretical: 10.153833 KDa

Sequence

String:

MAVKIRLKRM GAKKSPFYRI VVADSRSPRD GRFIETVGTY NPVAKPAEVK IDEELALKWL QTGAKPSDTV RNLFSSQGIM EKFHNAKQG K

UniProtKB: Small ribosomal subunit protein bS16

Macromolecule #42: 30S ribosomal protein S17

• Macromolecule: Name: 30S ribosomal protein S17 / type: protein_or_peptide / ID: 42 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Bacillus subtilis subsp. subtilis str. 168 (bacteria)
• Molecular weight: Theoretical: 10.220979 KDa

Sequence

String:

MSERNQRKVY QGRVVSDKMD KTITVVVETY KKHTLYGKRV KYSKKFKAHD ENNQAKIGDI VKIMETRPLS ATKRFRLVEV VEEAVII

UniProtKB: Small ribosomal subunit protein uS17

Macromolecule #43: 30S ribosomal protein S18

• Macromolecule: Name: 30S ribosomal protein S18 / type: protein_or_peptide / ID: 43 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Bacillus subtilis subsp. subtilis str. 168 (bacteria)
• Molecular weight: Theoretical: 8.990613 KDa

Sequence

String:

MAGGRRGGRA KRRKVCYFTS NGITHIDYKD VDLLKKFVSE RGKILPRRVT GTNAKYQRKL TAAIKRARQM ALLPYVSGE

UniProtKB: Small ribosomal subunit protein bS18

Macromolecule #44: 30S ribosomal protein S20

• Macromolecule: Name: 30S ribosomal protein S20 / type: protein_or_peptide / ID: 44 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Bacillus subtilis subsp. subtilis str. 168 (bacteria)
• Molecular weight: Theoretical: 9.622217 KDa

Sequence

String:

MPNIKSAIKR TKTNNERRVH NATIKSAMRT AIKQVEASVA NNEADKAKTA LTEAAKRIDK AVKTGLVHKN TAARYKSRLA KKVNGLSA

UniProtKB: Small ribosomal subunit protein bS20

Macromolecule #47: 30S ribosomal protein S2

• Macromolecule: Name: 30S ribosomal protein S2 / type: protein_or_peptide / ID: 47 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Bacillus subtilis subsp. subtilis str. 168 (bacteria)
• Molecular weight: Theoretical: 28.009297 KDa

Sequence

String:

MSVISMKQLL EAGVHFGHQT RRWNPKMKRY IFTERNGIYI IDLQKTVKKV EEAYNFTKNL AAEGGKILFV GTKKQAQDSV KEEAQRSGM YYVNQRWLGG TLTNFETIQK RIKRLKDIEK MQENGTFDVL PKKEVVQLKK ELERLEKFLG GIKDMKDLPD A LFIIDPRK ERIAVAEARK LNIPIIGIVD TNCDPDEIDV VIPANDDAIR AVKLLTSKMA DAILEAKQGE EEAEVAEETA PE TETTTA

UniProtKB: Small ribosomal subunit protein uS2

Macromolecule #48: Small ribosomal subunit protein uS19

• Macromolecule: Name: Small ribosomal subunit protein uS19 / type: protein_or_peptide / ID: 48 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Bacillus subtilis subsp. subtilis str. 168 (bacteria)
• Molecular weight: Theoretical: 10.607309 KDa

Sequence

String:

MARSLKKGPF VDGHLMTKIE KLNETDKKQV VKTWSRRSTI FPQFIGHTIA VYDGRKHVPV FISEDMVGHK LGEFAPTRTY KGHASDDKK TRR

UniProtKB: Small ribosomal subunit protein uS19

Macromolecule #49: Nascent Chain CliM

• Macromolecule: Name: Nascent Chain CliM / type: protein_or_peptide / ID: 49 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Clostridioides difficile 630 (bacteria)
• Molecular weight: Theoretical: 4.885685 KDa
• Recombinant expression: Organism: Bacillus subtilis subsp. subtilis str. 168 (bacteria)

Sequence

String:

YVLIRDIFVN RITYSEERLP KQYIVFQKYD IWRYCSLF

UniProtKB: Uncharacterized protein

Macromolecule #52: 30S ribosomal protein S4

• Macromolecule: Name: 30S ribosomal protein S4 / type: protein_or_peptide / ID: 52 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Bacillus subtilis subsp. subtilis str. 168 (bacteria)
• Molecular weight: Theoretical: 22.874271 KDa

Sequence

String:

MARYTGPSWK LSRRLGISLS GTGKELEKRP YAPGPHGPGQ RKKLSEYGLQ LQEKQKLRHM YGVNERQFRT LFDKAGKLAG KHGENFMIL LDSRLDNVVY KLGLARTRRQ ARQLVNHGHI LVDGSRVDIP SYLVKPGQTI GVREKSRNLS IIKESVEVNN F VPEYLTFD AEKLEGTFTR LPERSELAPE INEALIVEFY SR

UniProtKB: Small ribosomal subunit protein uS4

Macromolecule #7: 5S rRNA

• Macromolecule: Name: 5S rRNA / type: rna / ID: 7 / Number of copies: 1
• Source (natural): Organism: Bacillus subtilis subsp. subtilis str. 168 (bacteria)
• Molecular weight: Theoretical: 36.15752 KDa

Sequence

String:

UGGUGGCGAU AGCGAAGAGG UCACACCCGU UCCCAUACCG AACACGGAAG UUAAGCUCUU CAGCGCCGAU GGUAGUCGGG GGUUUCCCC CUGUGAGAGU AGGACGCCGC CAA

GENBANK: GENBANK: CP019662.1

Macromolecule #45: P-site tRNA-Phe

• Macromolecule: Name: P-site tRNA-Phe / type: rna / ID: 45 / Number of copies: 1
• Source (natural): Organism: Bacillus subtilis subsp. subtilis str. 168 (bacteria)
• Molecular weight: Theoretical: 24.485539 KDa

Sequence

String:

GGCUCGGUAG CUCAGUUGGU AGAGCAACGG ACUGAAAAUC CGUGUGUCGG CGGUUCGAUU CCGUCCCGAG CCACCA

Macromolecule #46: 23S rRNA

• Macromolecule: Name: 23S rRNA / type: rna / ID: 46 / Number of copies: 1
• Source (natural): Organism: Bacillus subtilis subsp. subtilis str. 168 (bacteria)
• Molecular weight: Theoretical: 949.683312 KDa

Sequence

String:

GGUUAAGUUA GAAAGGGCGC ACGGUGGAUG CCUUGGCACU AGGAGCCGAU GAAGGACGGG ACGAACACCG AUAUGCUUCG GGGAGCUGU AAGCAAGCUU UGAUCCGGAG AUUUCCGAAU GGGGAAACCC ACCACUCGUA AUGGAGUGGU AUCCAUAUCU G AAUUCAUA GGAUAUGAGA AGGCAGACCC GGGGAACUGA AACAUCUAAG UACCCGGAGG AAGAGAAAGC AAAUGCGAUU CC CUGAGUA GCGGCGAGCG AAACGGGAUC AGCCCAAACC AAGAGGCUUG CCUCUUGGGG UUGUAGGACA CUCUGUACGG AGU UACAAA GGAACGAGGU AGAUGAAGAG GUCUGGAAAG GCCCGCCAUA GGAGGUAACA GCCCUGUAGU CAAAACUUCG UUCU CUCCU GAGUGGAUCC UGAGUACGGC GGAACACGUG AAAUUCCGUC GGAAUCCGGG AGGACCAUCU CCCAAGGCUA AAUAC UCCC UAGUGACCGA UAGUGAACCA GUACCGUGAG GGAAAGGUGA AAAGCACCCC GGAAGGGGAG UGAAAGAGAU CCUGAA ACC GUGUGCCUAC AAGUAGUCAG AGCCCGUUAA CGGGUGAUGG CGUGCCUUUU GUAGAAUGAA CCGGCGAGUU ACGAUCU CG UGCAAGGUUA AGCAGAAGAG ACGGAGCCGC AGCGAAAGCG AGUCUGAAUA GGGCGCAUGA GUACGUGGUC GUAGACCC G AAACCAGGUG AUCUACCCAU GUCCAGGGUG AAGUUCAGGU AACACUGAAU GGAGGCCCGA ACCCACGCAC GUUGAAAAG UGCGGGGAUG AGGUGUGGGU AGGGGUGAAA UGCCAAUCGA ACCUGGAGAU AGCUGGUUCU CUCCGAAAUA GCUUUAGGGC UAGCCUCAA GGUAAGAGUC UUGGAGGUAG AGCACUGAUU GGACUAGGGG CCCCUACCGG GUUACCGAAU UCAGUCAAAC U CCGAAUGC CAAUGACUUA UCCUUGGGAG UCAGACUGCG AGUGAUAAGA UCCGUAGUCG AAAGGGAAAC AGCCCAGACC GC CAGCUAA GGUCCCAAAG UAUACGUUAA GUGGAAAAGG AUGUGGAGUU GCUUAGACAA CCAGGAUGUU GGCUUAGAAG CAG CCACCA UUUAAAGAGU GCGUAAUAGC UCACUGGUCG AGUGACUCUG CGCCGAAAAU GUACCGGGGC UAAACGUAUC ACCG AAGCU GCGGACUGUU CUUCGAACAG UGGUAGGAGA GCGUUCUAAG GGCUGUGAAG CGAGACCGGA AGGACUCGUG GAGCG CUUA GAAGUGAGAA UGCCGGUAUG AGUAGCGAAA GAGGGGUGAG AAUCCCCUCC ACCGAAUGCC UAAGGUUUCC UGAGGA AGG CUCGUCCGCU CAGGGUUAGU CGGGACCUAA GCCGAGGCCG AAAGGCGUAG GCGAUGGACA ACAGGUUGAU AUUCCUG UA CCACCUCCUC ACCAUUUGAG CAAUGGGGGG ACGCAGGAGG AUAGGGUAAG CGCGGUAUUG GAUAUCCGCG UCCAAGCA G UUAGGCUGGG AAAUAGGCAA AUCCGUUUCC CAUAAGGCUG AGCUGUGAUG GCGAGCGAAA UAUAGUAGCG AAGUUCCUG AUUCCACACU GCCAAGAAAA GCCUCUAGCG AGGUGAGAGG UGCCCGUACC GCAAACCGAC ACAGGUAGGC GAGGAGAGAA UCCUAAGGU GAUCGAGAGA ACUCUCGUUA AGGAACUCGG CAAAAUGACC CCGUAACUUC GGGAGAAGGG GUGCUCUGUU A GGGUGCAA GCCCGAGAGA GCCGCAGUGA AUAGGCCCAG GCGACUGUUU AGCAAAAACA CAGGUCUCUG CGAAGCCGUA AG GCGAAGU AUAGGGGCUG ACGCCUGCCC GGUGCUGGAA GGUUAAGAGG AGCGCUUAGC GUAAGCGAAG GUGCGAAUUG AAG CCCCAG UAAACGGCGG CCGUAACUAU AACGGUCCUA AGGUAGCGAA AUUCCUUGUC GGGUAAGUUC CGACCCGCAC GAAA GGCGC AACGAUCUGG GCACUGUCUC AACGAGAGAC UCGGUGAAAU UAUAGUACCU GUGAAGAUGC AGGUUACCCG CGACA GGAC GGAAAGACCC CGUGGAGCUU UACUGCAGCC UGAUAUUGAA UGUUGGUACA GCUUGUACAG GAUAGGUAGG AGCCUU GGA AACCGGAGCG CCAGCUUCGG UGGAGGCAUC GGUGGGAUAC UACCCUGGCU GUAUUGACCU UCUAACCCGC CGCCCUU AU CGGGCGGGGA GACAGUGUCA GGUGGGCAGU UUGACUG(OMG)GG CGGUCGCCUC CUAAAAGGUA ACGGAGGCGC CCAA AGGUU CCCUCAGAAU GGUUGGAAAU CAUUCGCAGA GUGUAAAGGC ACAAGGGAGC UUGACUGCGA GACCUACAAG UCGAG CAGG GACGAAAGUC GGGCUUAGUG AUCCGGUGGU UCCGCAUGGA AGGGCCAUCG CUCAACGGAU AAAAGCUACC CCGGGG AUA ACAGGCUUAU CUCCCCCAAG AGUCCACAUC GACGGGGAGG UUUGGCACCU CGAUGUCGGC UCAUCGCAUC CUGGGGC UG UAGUCGGUCC CAAGGGUUGG GCUGUUCGCC CAUUAAAGCG GUACGCGAGC UGGGUUCAGA ACGUCGUGAG ACAGUUCG G UCCCUAUCCG UCGCGGGCGC AGGAAAUUUG AGAGGAGCUG UCCUUAGUAC GAGAGGACCG GGAUGGACGC ACCGCUGGU G(PSU)ACCAGUUG UUCUGCCAAG GGCAUCGCUG GGUAGCUAUG UGCGGACGGG AUAAGUGCUG AAAGCAUCUA AGCAUG AAG CCCCCCUCAA GAUGAGAUUU CCCAUUCCGC AAGGAAGUAA GAUCCCUGAA AGAUGAUCAG GUUGAUAGGU CUGAGGU GG AAGUGUGGCG ACACAUGGAG CUGACAGAUA CUAAUCGAUC GAGGACUUAA CCAU

Macromolecule #50: mRNA

• Macromolecule: Name: mRNA / type: rna / ID: 50 / Number of copies: 1
• Source (natural): Organism: Clostridioides difficile 630 (bacteria)
• Molecular weight: Theoretical: 1.831078 KDa

Sequence

String:

UUGUUU

Macromolecule #51: 16S rRNA

• Macromolecule: Name: 16S rRNA / type: rna / ID: 51 / Number of copies: 1
• Source (natural): Organism: Bacillus subtilis subsp. subtilis str. 168 (bacteria)
• Molecular weight: Theoretical: 503.369125 KDa

Sequence

String:

UUUAUCGGAG AGUUUGAUCC UGGCUCAGGA CGAACGCUGG CGGCGUGCCU AAUACAUGCA AGUCGAGCGG ACAGAUGGGA GCUUGCUCC CUGAUGUUAG CGGCGGACGG GUGAGUAACA CGUGGGUAAC CUGCCUGUAA GACUGGGAUA ACUCCGGGAA A CCGGGGCU AAUACCGGAU GGUUGUUUGA ACCGCAUGGU UCAAACAUAA AAGGUGGCUU CGGCUACCAC UUACAGAUGG AC CCGCGGC GCAUUAGCUA GUUGGUGAGG UAACGGCUCA CCAAGGCGAC GAUGCGUAGC CGACCUGAGA GGGUGAUCGG CCA CACUGG GACUGAGACA CGGCCCAGAC UCCUACGGGA GGCAGCAGUA GGGAAUCUUC CGCAAUGGAC GAAAGUCUGA CGGA GCAAC GCCGCGUGAG UGAUGAAGGU UUUCGGAUCG UAAAGCUCUG UUGUUAGGGA AGAACAAGUG CCGUUCGAAU AGGGC GGUA CCUUGACGGU ACCUAACCAG AAAGCCACGG CUAACUACGU GCCAGCAGCC GCGGUAAUAC GUAGGUGGCA AGCGUU GUC CGGAAUUAUU GGGCGUAAAG GGCUCGCAGG CGGUUUCUUA AGUCUGAUGU GAAAGCCCCC GGCUCAACCG GGGAGGG UC AUUGGAAACU GGGGAACUUG AGUGCAGAAG AGGAGAGUGG AAUUCCACGU GUAGCGGUGA AAUGCGUAGA GAUGUGGA G GAACACCAGU GGCGAAGGCG ACUCUCUGGU CUGUAACUGA CGCUGAGGAG CGAAAGCGUG GGGAGCGAAC AGGAUUAGA UACCCUGGUA GUCCACGCCG UAAACGAUGA GUGCUAAGUG UUAGGGGGUU UCCGCCCCUU AGUGCUGCAG CUAACGCAUU AAGCACUCC GCCUGGGGAG UACGGUCGCA AGACUGAAAC UCAAAGGAAU UGACGGGGGC CCGCACAAGC GGUGGAGCAU G UGGUUUAA UUCGAAGCAA CGCGAAGAAC CUUACCAGGU CUUGACAUCC UCUGACAAUC CUAGAGAUAG GACGUCCCCU UC GGGGGCA GAGUGACAGG UGGUGCAUGG UUGUCGUCAG CUCGUGUCGU GAGAUGUUGG GUUAAGUCCC GCAACGAGCG CAA CCCUUG AUCUUAGUUG CCAGCAUUCA GUUGGGCACU CUAAGGUGAC UGCCGGUGAC AAACCGGAGG AAGGUGGGGA UGAC GUCAA AUCAUCAUGC CCCUUAUGAC CUGGGCUACA CACGUGCUAC AAUGGACAGA ACAAAGGGCA GCGAAACCGC GAGGU UAAG CCAAUCCCAC AAAUCUGUUC UCAGUUCGGA UCGCAGUCUG CAACUCGACU GCGUGAAGCU GGAAUCGCUA GUAAUC GCG GAUCAGCAUG CCGCGGUGAA UACGUUCCCG GGCCUUGUAC ACACCGCCCG UCACACCACG AGAGUUUGUA ACACCCG AA GUCGGUGAGG UAACCUUUUA GGAGCCAGCC GCCGAAGGUG GGACAGAUGA UUGGGGUGAA GUCGUAACAA GGUAGCCG U AUCGGAAGGU GCGGCUGGAU CACCUCCUUU CUA

GENBANK: GENBANK: AL009126.3

Experimental details

Structure determination

• Method: cryo EM
• Processing: single particle reconstruction
• Aggregation state: particle

Sample preparation

• Buffer: pH: 7.4
• Vitrification: Cryogen name: ETHANE-PROPANE

Electron microscopy

• Microscope: TFS KRIOS
• Image recording: Film or detector model: GATAN K3 BIOQUANTUM (6k x 4k) / Average electron dose: 1.14 e/Ų
• Electron beam: Acceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
• Electron optics: Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Nominal defocus max: 0.9 µm / Nominal defocus min: 0.3 µm
• Experimental equipment: Model: Titan Krios / Image courtesy: FEI Company

Image processing

• CTF correction: Type: PHASE FLIPPING AND AMPLITUDE CORRECTION

Startup model

Type of model: PDB ENTRY
PDB model - PDB ID:

6ha8

• Final reconstruction: Resolution.type: BY AUTHOR / Resolution: 2.3 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 368433
• Initial angle assignment: Type: MAXIMUM LIKELIHOOD
• Final angle assignment: Type: MAXIMUM LIKELIHOOD