[English] 日本語
Yorodumi- EMDB-50022: Influenza virus neuraminidase N1 NC13 ectodomain with a tetrabrac... -
+Open data
-Basic information
Entry | Database: EMDB / ID: EMD-50022 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
Title | Influenza virus neuraminidase N1 NC13 ectodomain with a tetrabrachio-domain stalk | |||||||||
Map data | ||||||||||
Sample |
| |||||||||
Keywords | Neuraminidase / Tetrabrachion / VIRAL PROTEIN | |||||||||
Function / homology | Function and homology information : / : / : / exo-alpha-sialidase / viral budding from plasma membrane / carbohydrate metabolic process / host cell plasma membrane / virion membrane / membrane / metal ion binding Similarity search - Function | |||||||||
Biological species | Influenza A virus (A/North Carolina/07/2013(H1N1)) / Influenza A virus | |||||||||
Method | single particle reconstruction / cryo EM / Resolution: 3.0 Å | |||||||||
Authors | Roelofs MC / Zeev-Ben-Mordehai T | |||||||||
Funding support | 1 items
| |||||||||
Citation | Journal: NPJ Vaccines / Year: 2024 Title: Nanoparticle display of neuraminidase elicits enhanced antibody responses and protection against influenza A virus challenge. Authors: M N Pascha / M Ballegeer / M C Roelofs / L Meuris / I C Albulescu / F J M van Kuppeveld / D L Hurdiss / B J Bosch / T Zeev-Ben-Mordehai / X Saelens / C A M de Haan / Abstract: Current Influenza virus vaccines primarily induce antibody responses against variable epitopes in hemagglutinin (HA), necessitating frequent updates. However, antibodies against neuraminidase (NA) ...Current Influenza virus vaccines primarily induce antibody responses against variable epitopes in hemagglutinin (HA), necessitating frequent updates. However, antibodies against neuraminidase (NA) can also confer protection against influenza, making NA an attractive target for the development of novel vaccines. In this study, we aimed to enhance the immunogenicity of recombinant NA antigens by presenting them multivalently on a nanoparticle carrier. Soluble tetrameric NA antigens of the N1 and N2 subtypes, confirmed to be correctly folded by cryo-electron microscopy structural analysis, were conjugated to Mi3 self-assembling protein nanoparticles using the SpyTag-SpyCatcher system. Immunization of mice with NA-Mi3 nanoparticles induced higher titers of NA-binding and -inhibiting antibodies and improved protection against a lethal challenge compared to unconjugated NA. Additionally, we explored the co-presentation of N1 and N2 antigens on the same Mi3 particles to create a mosaic vaccine candidate. These mosaic nanoparticles elicited antibody titers that were similar or superior to the homotypic nanoparticles and effectively protected against H1N1 and H3N2 challenge viruses. The NA-Mi3 nanoparticles represent a promising vaccine candidate that could complement HA-directed approaches for enhanced potency and broadened protection against influenza A virus. | |||||||||
History |
|
-Structure visualization
Supplemental images |
---|
-Downloads & links
-EMDB archive
Map data | emd_50022.map.gz | 111.5 MB | EMDB map data format | |
---|---|---|---|---|
Header (meta data) | emd-50022-v30.xml emd-50022.xml | 13.8 KB 13.8 KB | Display Display | EMDB header |
FSC (resolution estimation) | emd_50022_fsc.xml | 12.7 KB | Display | FSC data file |
Images | emd_50022.png | 116.3 KB | ||
Filedesc metadata | emd-50022.cif.gz | 5.4 KB | ||
Others | emd_50022_half_map_1.map.gz emd_50022_half_map_2.map.gz | 200.1 MB 200.1 MB | ||
Archive directory | http://ftp.pdbj.org/pub/emdb/structures/EMD-50022 ftp://ftp.pdbj.org/pub/emdb/structures/EMD-50022 | HTTPS FTP |
-Validation report
Summary document | emd_50022_validation.pdf.gz | 942 KB | Display | EMDB validaton report |
---|---|---|---|---|
Full document | emd_50022_full_validation.pdf.gz | 941.6 KB | Display | |
Data in XML | emd_50022_validation.xml.gz | 20.5 KB | Display | |
Data in CIF | emd_50022_validation.cif.gz | 25.6 KB | Display | |
Arichive directory | https://ftp.pdbj.org/pub/emdb/validation_reports/EMD-50022 ftp://ftp.pdbj.org/pub/emdb/validation_reports/EMD-50022 | HTTPS FTP |
-Related structure data
Related structure data | 9ewqMC M: atomic model generated by this map C: citing same article (ref.) |
---|---|
Similar structure data | Similarity search - Function & homologyF&H Search |
-Links
EMDB pages | EMDB (EBI/PDBe) / EMDataResource |
---|---|
Related items in Molecule of the Month |
-Map
File | Download / File: emd_50022.map.gz / Format: CCP4 / Size: 216 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) | ||||||||||||||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Projections & slices | Image control
Images are generated by Spider. | ||||||||||||||||||||||||||||||||||||
Voxel size | X=Y=Z: 1.041 Å | ||||||||||||||||||||||||||||||||||||
Density |
| ||||||||||||||||||||||||||||||||||||
Symmetry | Space group: 1 | ||||||||||||||||||||||||||||||||||||
Details | EMDB XML:
|
-Supplemental data
-Half map: #1
File | emd_50022_half_map_1.map | ||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Projections & Slices |
| ||||||||||||
Density Histograms |
-Half map: #2
File | emd_50022_half_map_2.map | ||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Projections & Slices |
| ||||||||||||
Density Histograms |
-Sample components
-Entire : Influenza N1 NC13 with part of stalk replaced by tetrabrachion te...
Entire | Name: Influenza N1 NC13 with part of stalk replaced by tetrabrachion tetramerization domain |
---|---|
Components |
|
-Supramolecule #1: Influenza N1 NC13 with part of stalk replaced by tetrabrachion te...
Supramolecule | Name: Influenza N1 NC13 with part of stalk replaced by tetrabrachion tetramerization domain type: complex / ID: 1 / Parent: 0 / Macromolecule list: all |
---|---|
Source (natural) | Organism: Influenza A virus (A/North Carolina/07/2013(H1N1)) |
-Macromolecule #1: Neuraminidase
Macromolecule | Name: Neuraminidase / type: protein_or_peptide / ID: 1 / Number of copies: 4 / Enantiomer: LEVO / EC number: exo-alpha-sialidase |
---|---|
Source (natural) | Organism: Influenza A virus |
Molecular weight | Theoretical: 42.385258 KDa |
Recombinant expression | Organism: Homo sapiens (human) |
Sequence | String: VKLAGNSSLC PVSGWAIYSK DNSVRIGSKG DVFVIREPFI SCSPLECRTF FLTQGALLND KHSNGTIKDR SPYRTLMSCP IGEVPSPYN SRFESVAWSA SACHDGINWL TIGISGPDSG AVAVLKYNGI ITDTIKSWRN NILRTQESEC ACVNGSCFTI M TDGPSDGQ ...String: VKLAGNSSLC PVSGWAIYSK DNSVRIGSKG DVFVIREPFI SCSPLECRTF FLTQGALLND KHSNGTIKDR SPYRTLMSCP IGEVPSPYN SRFESVAWSA SACHDGINWL TIGISGPDSG AVAVLKYNGI ITDTIKSWRN NILRTQESEC ACVNGSCFTI M TDGPSDGQ ASYKIFRIEK GKIVKSVEMN APNYHYEECS CYPDSSEITC VCRDNWHGSN RPWVSFNQNL EYQIGYICSG VF GDNPRPN DKTGSCGPVS SNGANGVKGF SFKYGNGVWI GRTKSISSRK GFEMIWDPNG WTGTDNNFSI KQDIVGINEW SGY SGSFVQ HPELTGLDCI RPCFWVELIR GRPEENTIWT SGSSISFCGV NSDTVGWSWP DGAELPFTI UniProtKB: Neuraminidase |
-Experimental details
-Structure determination
Method | cryo EM |
---|---|
Processing | single particle reconstruction |
Aggregation state | particle |
-Sample preparation
Buffer | pH: 7.5 |
---|---|
Vitrification | Cryogen name: ETHANE-PROPANE |
-Electron microscopy
Microscope | FEI TALOS ARCTICA |
---|---|
Image recording | Film or detector model: GATAN K2 SUMMIT (4k x 4k) / Average exposure time: 0.2 sec. / Average electron dose: 48.1 e/Å2 |
Electron beam | Acceleration voltage: 200 kV / Electron source: FIELD EMISSION GUN |
Electron optics | Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Cs: 2.7 mm / Nominal defocus max: 3.0 µm / Nominal defocus min: 1.0 µm |
Experimental equipment | Model: Talos Arctica / Image courtesy: FEI Company |