National Institutes of Health/National Institute Of Allergy and Infectious Diseases (NIH/NIAID)
R01AI150524
United States
Citation
Journal: Protein Cell / Year: 2024 Title: Structural basis for polyuridine tract recognition by SARS-CoV-2 Nsp15. Authors: Fumiaki Ito / Hanjing Yang / Z Hong Zhou / Xiaojiang S Chen /
Name: RNA (35-MER) / type: rna / ID: 2 / Number of copies: 1
Source (natural)
Organism: Severe acute respiratory syndrome coronavirus 2
Molecular weight
Theoretical: 10.837149 KDa
Sequence
String:
UUUUUUUUUU UUUUUUUUUU GUCAUUCUCC UAAGA
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Macromolecule #3: RNA (35-MER)
Macromolecule
Name: RNA (35-MER) / type: rna / ID: 3 / Number of copies: 1
Source (natural)
Organism: Severe acute respiratory syndrome coronavirus 2
Molecular weight
Theoretical: 11.401036 KDa
Sequence
String:
UCUUAGGAGA AUGACAAAAA AAAAAAAAAA AAAAA
-
Experimental details
-
Structure determination
Method
cryo EM
Processing
single particle reconstruction
Aggregation state
particle
-
Sample preparation
Concentration
0.3 mg/mL
Buffer
pH: 7.5
Vitrification
Cryogen name: ETHANE / Chamber humidity: 100 % / Chamber temperature: 277 K / Instrument: FEI VITROBOT MARK IV
-
Electron microscopy
Microscope
TFS GLACIOS
Image recording
Film or detector model: FEI FALCON IV (4k x 4k) / Digitization - Dimensions - Width: 4096 pixel / Digitization - Dimensions - Height: 4096 pixel / Number grids imaged: 2 / Number real images: 15151 / Average exposure time: 8.0 sec. / Average electron dose: 52.0 e/Å2
Electron beam
Acceleration voltage: 200 kV / Electron source: FIELD EMISSION GUN
In the structure databanks used in Yorodumi, some data are registered as the other names, "COVID-19 virus" and "2019-nCoV". Here are the details of the virus and the list of structure data.
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