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- EMDB-41431: 48-nm repeating structure of doublets from mouse sperm flagella -

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Basic information

Entry
Database: EMDB / ID: EMD-41431
Title48-nm repeating structure of doublets from mouse sperm flagella
Map dataFinal map of 48 nm-repeating unit of mouse sperm doublets
Sample
  • Cell: Mouse sperm
    • Protein or peptide: x 35 types
KeywordsMammalian sperm / axoneme / microtubule-based structure / microtubule inner protein / non-motor proteins / cellular motility / fertility / structural protein
Function / homology
Function and homology information


ERKs are inactivated / 9+0 motile cilium / sperm flagellum assembly / outer acrosomal membrane / regulation of brood size / establishment of left/right asymmetry / protein localization to motile cilium / manchette assembly / Microtubule-dependent trafficking of connexons from Golgi to the plasma membrane / Cilium Assembly ...ERKs are inactivated / 9+0 motile cilium / sperm flagellum assembly / outer acrosomal membrane / regulation of brood size / establishment of left/right asymmetry / protein localization to motile cilium / manchette assembly / Microtubule-dependent trafficking of connexons from Golgi to the plasma membrane / Cilium Assembly / Sealing of the nuclear envelope (NE) by ESCRT-III / left/right pattern formation / axonemal B tubule inner sheath / axonemal A tubule inner sheath / epithelial cilium movement involved in determination of left/right asymmetry / regulation of calcineurin-NFAT signaling cascade / MAP kinase phosphatase activity / inner dynein arm assembly / Intraflagellar transport / Carboxyterminal post-translational modifications of tubulin / protein polyglutamylation / positive regulation of feeding behavior / cerebrospinal fluid circulation / sperm axoneme assembly / COPI-independent Golgi-to-ER retrograde traffic / cilium-dependent cell motility / sperm principal piece / HSP90 chaperone cycle for steroid hormone receptors (SHR) in the presence of ligand / regulation of cilium beat frequency involved in ciliary motility / cilium movement involved in cell motility / regulation of store-operated calcium entry / epithelial cilium movement involved in extracellular fluid movement / 9+2 motile cilium / intraciliary transport / PKR-mediated signaling / negative regulation of chemotaxis / COPI-mediated anterograde transport / calcium ion sensor activity / Aggrephagy / Transferases; Transferring phosphorus-containing groups / acrosomal membrane / Kinesins / ciliary transition zone / Mitotic Prometaphase / EML4 and NUDC in mitotic spindle formation / cilium movement / Resolution of Sister Chromatid Cohesion / RHO GTPases activate IQGAPs / The role of GTSE1 in G2/M progression after G2 checkpoint / Recycling pathway of L1 / axoneme assembly / axonemal microtubule / negative regulation of T cell activation / left/right axis specification / cilium organization / COPI-dependent Golgi-to-ER retrograde traffic / gamma-tubulin ring complex / flagellated sperm motility / RHO GTPases Activate Formins / Separation of Sister Chromatids / Hedgehog 'off' state / organelle transport along microtubule / glial cell differentiation / Loss of Nlp from mitotic centrosomes / Recruitment of mitotic centrosome proteins and complexes / Loss of proteins required for interphase microtubule organization from the centrosome / protein tyrosine/serine/threonine phosphatase activity / Recruitment of NuMA to mitotic centrosomes / Anchoring of the basal body to the plasma membrane / AURKA Activation by TPX2 / forebrain morphogenesis / neuron projection arborization / Regulation of PLK1 Activity at G2/M Transition / positive regulation of focal adhesion disassembly / manchette / cerebellar cortex morphogenesis / positive regulation of cilium assembly / dentate gyrus development / MHC class II antigen presentation / pyramidal neuron differentiation / negative regulation of JNK cascade / CTP biosynthetic process / UTP biosynthetic process / motile cilium / positive regulation of cell motility / determination of left/right symmetry / centrosome cycle / motor behavior / GTP biosynthetic process / microtubule organizing center / response to L-glutamate / intermediate filament / regulation of neuron projection development / ciliary base / nucleoside diphosphate kinase activity / regulation of focal adhesion assembly / tubulin complex / negative regulation of T cell receptor signaling pathway / negative regulation of MAPK cascade / smoothened signaling pathway
Similarity search - Function
DJBP, EF-hand domain / Tektin bundle interacting protein 1 / Coiled-coil domain-containing protein 105 / EF-hand domain / Tektin bundle interacting protein 1 / Atypical dual specificity phosphatase, subfamily A / Sperm-associated antigen 8 / Sperm acrosome-associated protein 9 / Cilia- and flagella-associated protein 95 / NDPK7, first NDPk domain ...DJBP, EF-hand domain / Tektin bundle interacting protein 1 / Coiled-coil domain-containing protein 105 / EF-hand domain / Tektin bundle interacting protein 1 / Atypical dual specificity phosphatase, subfamily A / Sperm-associated antigen 8 / Sperm acrosome-associated protein 9 / Cilia- and flagella-associated protein 95 / NDPK7, first NDPk domain / Cilia- and flagella-associated protein 107 / : / Sperm acrosome-associated protein 9 / Protein CFAP95 / Tektin / Cilia- and flagella-associated protein 276 / Tektin family / Protein of unknown function (DUF3695) / Uncharacterised protein FAM166/UPF0605 / Protein Flattop / Ciliary microtubule inner protein 2B-like / Nucleoside diphosphate kinase 7 / Meiosis-specific nuclear structural protein 1 / Piercer of microtubule wall 1/2 / Cilia- and flagella-associated protein 141 / Cilia- and flagella-associated protein 45 / NDPK7, second NDPk domain / Cilia- and flagella- associated protein 210 / CFAP53/TCHP / Trichohyalin-plectin-homology domain / Trichohyalin-plectin-homology domain / Piercer of microtubule wall 1/2 / Cilia- and flagella-associated protein 141 / RIB43A / RIB43A / DM10 domain / EF-hand domain-containing protein EFHC1/EFHC2/EFHB / DM10 domain / DM10 domain profile. / Domains in hypothetical proteins in Drosophila, C. elegans and mammals. Occurs singly in some nucleoside diphosphate kinases. / Enkurin domain / Calmodulin-binding / Enkurin domain profile. / CFA20 domain / Cilia- and flagella-associated protein 20/CFAP20DC / CFA20 domain / Parkin co-regulated protein / Parkin co-regulated protein / Dual specificity phosphatase, catalytic domain / Nucleoside diphosphate kinase (NDPK)-like domain profile. / EF hand / Dual specificity phosphatase, catalytic domain / Dual specificity phosphatase, catalytic domain / Dual specificity protein phosphatase domain / Dual specificity protein phosphatase domain profile. / Nucleoside diphosphate kinase / Nucleoside diphosphate kinase-like domain / Nucleoside diphosphate kinase / NDK / Nucleoside diphosphate kinase-like domain superfamily / EF-hand domain pair / Mir domain superfamily / Alpha tubulin / Tubulin-beta mRNA autoregulation signal. / Beta tubulin, autoregulation binding site / Beta tubulin / Tubulin / Tubulin, C-terminal / Tubulin C-terminal domain / Tubulin, conserved site / Tubulin subunits alpha, beta, and gamma signature. / Tyrosine specific protein phosphatases active site. / Protein-tyrosine phosphatase, active site / Tyrosine-specific protein phosphatases domain / Tyrosine specific protein phosphatases domain profile. / Tubulin/FtsZ family, C-terminal domain / Tubulin/FtsZ-like, C-terminal domain / Tubulin/FtsZ, C-terminal / Tubulin/FtsZ, 2-layer sandwich domain / Tubulin/FtsZ family, GTPase domain / Tubulin/FtsZ family, GTPase domain / Tubulin/FtsZ, GTPase domain / Tubulin/FtsZ, GTPase domain superfamily / Protein-tyrosine phosphatase-like / EF-hand domain pair / EF-hand, calcium binding motif / EF-Hand 1, calcium-binding site / EF-hand calcium-binding domain. / EF-hand calcium-binding domain profile. / EF-hand domain / EF-hand domain pair / WD40 repeat, conserved site / Trp-Asp (WD) repeats signature. / Trp-Asp (WD) repeats profile. / Trp-Asp (WD) repeats circular profile. / WD domain, G-beta repeat / WD40 repeats / WD40 repeat / WD40-repeat-containing domain superfamily / WD40/YVTN repeat-like-containing domain superfamily
Similarity search - Domain/homology
Cilia- and flagella- associated protein 210 / Ciliary microtubule inner protein 2B / Tektin bundle-interacting protein 1 / Tektin-5 / Tubulin alpha-1A chain / Tubulin beta-4B chain / Tektin-4 / Sperm-associated antigen 8 / Cilia- and flagella-associated protein 107 / Piercer of microtubule wall 1 protein ...Cilia- and flagella- associated protein 210 / Ciliary microtubule inner protein 2B / Tektin bundle-interacting protein 1 / Tektin-5 / Tubulin alpha-1A chain / Tubulin beta-4B chain / Tektin-4 / Sperm-associated antigen 8 / Cilia- and flagella-associated protein 107 / Piercer of microtubule wall 1 protein / Cilia- and flagella-associated protein 52 / Meiosis-specific nuclear structural protein 1 / EF-hand calcium-binding domain-containing protein 6 / Protein Flattop / Cilia- and flagella-associated protein 161 / Enkurin / Tektin-3 / Sperm acrosome-associated protein 9 / Cilia- and flagella-associated protein 20 / EF-hand domain-containing family member B / Tektin-2 / Cilia- and flagella-associated protein 95 / Cilia- and flagella-associated protein 53 / EF-hand domain-containing family member C2 / Tektin-like protein 1 / RIB43A-like with coiled-coils protein 2 / Dual specificity protein phosphatase 3 / Cilia- and flagella-associated protein 141 / EF-hand domain-containing protein 1 / Cilia- and flagella-associated protein 45 / Cilia- and flagella-associated protein 276 / Tektin-1 / Parkin coregulated gene protein homolog / Nucleoside diphosphate kinase homolog 7 / Piercer of microtubule wall 2 protein
Similarity search - Component
Biological speciesMus musculus (house mouse)
Methodsubtomogram averaging / cryo EM / Resolution: 7.7 Å
AuthorsChen Z / Shiozak M / Hass KM / Skinner W / Zhao S / Guo C / Polacco BJ / Yu Z / Krogan NJ / Kaake RM ...Chen Z / Shiozak M / Hass KM / Skinner W / Zhao S / Guo C / Polacco BJ / Yu Z / Krogan NJ / Kaake RM / Vale RD / Agard DA
Funding support United States, 3 items
OrganizationGrant numberCountry
Howard Hughes Medical Institute (HHMI) United States
National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)GM118106 United States
National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)GM118099 United States
CitationJournal: Cell / Year: 2023
Title: De novo protein identification in mammalian sperm using in situ cryoelectron tomography and AlphaFold2 docking.
Authors: Zhen Chen / Momoko Shiozaki / Kelsey M Haas / Will M Skinner / Shumei Zhao / Caiying Guo / Benjamin J Polacco / Zhiheng Yu / Nevan J Krogan / Polina V Lishko / Robyn M Kaake / Ronald D Vale / David A Agard /
Abstract: To understand the molecular mechanisms of cellular pathways, contemporary workflows typically require multiple techniques to identify proteins, track their localization, and determine their ...To understand the molecular mechanisms of cellular pathways, contemporary workflows typically require multiple techniques to identify proteins, track their localization, and determine their structures in vitro. Here, we combined cellular cryoelectron tomography (cryo-ET) and AlphaFold2 modeling to address these questions and understand how mammalian sperm are built in situ. Our cellular cryo-ET and subtomogram averaging provided 6.0-Å reconstructions of axonemal microtubule structures. The well-resolved tertiary structures allowed us to unbiasedly match sperm-specific densities with 21,615 AlphaFold2-predicted protein models of the mouse proteome. We identified Tektin 5, CCDC105, and SPACA9 as novel microtubule-associated proteins. These proteins form an extensive interaction network crosslinking the lumen of axonemal doublet microtubules, suggesting their roles in modulating the mechanical properties of the filaments. Indeed, Tekt5 -/- sperm possess more deformed flagella with 180° bends. Together, our studies presented a cellular visual proteomics workflow and shed light on the in vivo functions of Tektin 5.
History
DepositionAug 2, 2023-
Header (metadata) releaseNov 1, 2023-
Map releaseNov 1, 2023-
UpdateNov 22, 2023-
Current statusNov 22, 2023Processing site: RCSB / Status: Released

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Structure visualization

Supplemental images

emd_41431.png

Downloads & links

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Map

FileDownload / File: emd_41431.map.gz / Format: CCP4 / Size: 80.2 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
AnnotationFinal map of 48 nm-repeating unit of mouse sperm doublets
Voxel sizeX=Y=Z: 2.65 Å
Density
Contour LevelBy AUTHOR: 0.05
Minimum - Maximum0.0 - 0.2232013
Average (Standard dev.)0.005260748 (±0.016480323)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions276276276
Spacing276276276
CellA=B=C: 731.4 Å
α=β=γ: 90.0 °

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Supplemental data

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Sample components

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Entire : Mouse sperm

EntireName: Mouse sperm
Components
  • Cell: Mouse sperm
    • Protein or peptide: Cilia- and flagella-associated protein 95
    • Protein or peptide: Tektin-1
    • Protein or peptide: Detyrosinated tubulin alpha-1A chain
    • Protein or peptide: Tubulin beta-4B chain
    • Protein or peptide: Tektin-2
    • Protein or peptide: EF-hand domain-containing family member B
    • Protein or peptide: Tektin-3
    • Protein or peptide: Meiosis-specific nuclear structural protein 1
    • Protein or peptide: Tektin-4
    • Protein or peptide: Cilia- and flagella-associated protein 53
    • Protein or peptide: Tektin bundle-interacting protein 1
    • Protein or peptide: Cilia- and flagella-associated protein 141
    • Protein or peptide: Nucleoside diphosphate kinase 7
    • Protein or peptide: Protein FAM166B
    • Protein or peptide: Sperm-associated antigen 8
    • Protein or peptide: RIB43A-like with coiled-coils protein 2
    • Protein or peptide: Cilia- and flagella-associated protein 107
    • Protein or peptide: Cilia- and flagella-associated protein 161
    • Protein or peptide: EF-hand domain-containing protein 1
    • Protein or peptide: Sperm acrosome-associated protein 9
    • Protein or peptide: EF-hand domain-containing family member C2
    • Protein or peptide: Cilia- and flagella-associated protein 20
    • Protein or peptide: Parkin coregulated gene protein homolog
    • Protein or peptide: Dual specificity protein phosphatase 3
    • Protein or peptide: Piercer of microtubule wall 2 protein
    • Protein or peptide: Tektin-5
    • Protein or peptide: Cilia- and flagella-associated protein 45
    • Protein or peptide: Cilia- and flagella-associated protein 52
    • Protein or peptide: Enkurin
    • Protein or peptide: Protein Flattop
    • Protein or peptide: Cilia- and flagella- associated protein 210
    • Protein or peptide: Cilia- and flagella-associated protein 276
    • Protein or peptide: EF-hand calcium-binding domain-containing protein 6
    • Protein or peptide: Coiled-coil domain-containing protein 105
    • Protein or peptide: Piercer of microtubule wall 1 protein

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Supramolecule #1: Mouse sperm

SupramoleculeName: Mouse sperm / type: cell / ID: 1 / Parent: 0 / Macromolecule list: all
Source (natural)Organism: Mus musculus (house mouse)

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Macromolecule #1: Cilia- and flagella-associated protein 95

MacromoleculeName: Cilia- and flagella-associated protein 95 / type: protein_or_peptide / ID: 1 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Mus musculus (house mouse)
Molecular weightTheoretical: 26.633035 KDa
SequenceString: MGTFDSQCHE CFESKERWYE IGPTDLLERK GSLTLRSHHK KYSEPVLVYS WHRNREAFPK DYDIESLETV KNLCMSTYKR FGSDSPRWM SEAREQMAQV LVNKDLVEKK KTGLLDEETL CPGILERDPE LPPTGFRDVP MESPPDPRKP CFSTTYSEDF T PQYEYPPP ...String:
MGTFDSQCHE CFESKERWYE IGPTDLLERK GSLTLRSHHK KYSEPVLVYS WHRNREAFPK DYDIESLETV KNLCMSTYKR FGSDSPRWM SEAREQMAQV LVNKDLVEKK KTGLLDEETL CPGILERDPE LPPTGFRDVP MESPPDPRKP CFSTTYSEDF T PQYEYPPP ACPRREEYSI VHRKCRSQFT DLNGSKRLGI NTWHDESGIY ANSEAKQKLY ALARNPIVPF

UniProtKB: Cilia- and flagella-associated protein 95

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Macromolecule #2: Tektin-1

MacromoleculeName: Tektin-1 / type: protein_or_peptide / ID: 2 / Number of copies: 5 / Enantiomer: LEVO
Source (natural)Organism: Mus musculus (house mouse)
Molecular weightTheoretical: 48.713035 KDa
SequenceString: MAKLLQSPPR FLPEEWYIAN KSQYHRAEAQ RSQSERLVAE SQRLVEEIEK TTRKSQSDVN KKLEQRLEEV RFWKKELDDK LEQLVNQTD DLLTYKTRLE RSLESYKEPL HITEKCLEYR EKRVGIDLVH DVVEQELQKE ADIIHGVMNL LIRTLEESTE Q IRLNRSAK ...String:
MAKLLQSPPR FLPEEWYIAN KSQYHRAEAQ RSQSERLVAE SQRLVEEIEK TTRKSQSDVN KKLEQRLEEV RFWKKELDDK LEQLVNQTD DLLTYKTRLE RSLESYKEPL HITEKCLEYR EKRVGIDLVH DVVEQELQKE ADIIHGVMNL LIRTLEESTE Q IRLNRSAK YNLEKDLRDK FTAITIDDVC FSLNNNSPNI NFSEKVVRIE PNSVSLEDWL DFSNANVEKA DKQLNNSTAL KT LVDQILS QTANDLRRQC EVVDEAFING LKETKDARNK LADHLAKVME EIASQEKNIM ALENAITQQE GPAKVAHTRL ETR THRPNV ELCRDIAQYR LIKEIQEINH NVARLKETLA QAQTQLKALY RRQLALQEEI QVKENTIYID QVLCMEMRKS IPPR DGDDH GAWEGGIRAE AIC

UniProtKB: Tektin-1

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Macromolecule #3: Detyrosinated tubulin alpha-1A chain

MacromoleculeName: Detyrosinated tubulin alpha-1A chain / type: protein_or_peptide / ID: 3 / Number of copies: 149 / Enantiomer: LEVO
Source (natural)Organism: Mus musculus (house mouse)
Molecular weightTheoretical: 50.188441 KDa
SequenceString: MRECISIHVG QAGVQIGNAC WELYCLEHGI QPDGQMPSDK TIGGGDDSFN TFFSETGAGK HVPRAVFVDL EPTVIDEVRT GTYRQLFHP EQLITGKEDA ANNYARGHYT IGKEIIDLVL DRIRKLADQC TGLQGFLVFH SFGGGTGSGF TSLLMERLSV D YGKKSKLE ...String:
MRECISIHVG QAGVQIGNAC WELYCLEHGI QPDGQMPSDK TIGGGDDSFN TFFSETGAGK HVPRAVFVDL EPTVIDEVRT GTYRQLFHP EQLITGKEDA ANNYARGHYT IGKEIIDLVL DRIRKLADQC TGLQGFLVFH SFGGGTGSGF TSLLMERLSV D YGKKSKLE FSIYPAPQVS TAVVEPYNSI LTTHTTLEHS DCAFMVDNEA IYDICRRNLD IERPTYTNLN RLIGQIVSSI TA SLRFDGA LNVDLTEFQT NLVPYPRIHF PLATYAPVIS AEKAYHEQLS VAEITNACFE PANQMVKCDP RHGKYMACCL LYR GDVVPK DVNAAIATIK TKRTIQFVDW CPTGFKVGIN YQPPTVVPGG DLAKVQRAVC MLSNTTAIAE AWARLDHKFD LMYA KRAFV HWYVGEGMEE GEFSEAREDM AALEKDYEEV GVDSVEGEGE EEGEEY

UniProtKB: Tubulin alpha-1A chain

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Macromolecule #4: Tubulin beta-4B chain

MacromoleculeName: Tubulin beta-4B chain / type: protein_or_peptide / ID: 4 / Number of copies: 153 / Enantiomer: LEVO
Source (natural)Organism: Mus musculus (house mouse)
Molecular weightTheoretical: 49.877824 KDa
SequenceString: MREIVHLQAG QCGNQIGAKF WEVISDEHGI DPTGTYHGDS DLQLERINVY YNEATGGKYV PRAVLVDLEP GTMDSVRSGP FGQIFRPDN FVFGQSGAGN NWAKGHYTEG AELVDSVLDV VRKEAESCDC LQGFQLTHSL GGGTGSGMGT LLISKIREEY P DRIMNTFS ...String:
MREIVHLQAG QCGNQIGAKF WEVISDEHGI DPTGTYHGDS DLQLERINVY YNEATGGKYV PRAVLVDLEP GTMDSVRSGP FGQIFRPDN FVFGQSGAGN NWAKGHYTEG AELVDSVLDV VRKEAESCDC LQGFQLTHSL GGGTGSGMGT LLISKIREEY P DRIMNTFS VVPSPKVSDT VVEPYNATLS VHQLVENTDE TYCIDNEALY DICFRTLKLT TPTYGDLNHL VSATMSGVTT CL RFPGQLN ADLRKLAVNM VPFPRLHFFM PGFAPLTSRG SQQYRALTVP ELTQQMFDAK NMMAACDPRH GRYLTVAAVF RGR MSMKEV DEQMLNVQNK NSSYFVEWIP NNVKTAVCDI PPRGLKMSAT FIGNSTAIQE LFKRISEQFT AMFRRKAFLH WYTG EGMDE MEFTEAESNM NDLVSEYQQY QDATAEEEGE FEEEAEEEVA

UniProtKB: Tubulin beta-4B chain

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Macromolecule #5: Tektin-2

MacromoleculeName: Tektin-2 / type: protein_or_peptide / ID: 5 / Number of copies: 10 / Enantiomer: LEVO
Source (natural)Organism: Mus musculus (house mouse)
Molecular weightTheoretical: 50.385066 KDa
SequenceString: MATLSFKPSE RYRLSDWRTN SYLLSTNAER QRDASHQIRQ EARILRNETN NQIVWDEHDN RTRLAERIDT VNRWKETLDK CLTDLDAEI DSLAQAKESA EQNLQAKNLP LDVAIECLTL RESRRDIDVV RDPVEEELLK EVEVIEATKK VLQEKISQAF Q HLCLLQEI ...String:
MATLSFKPSE RYRLSDWRTN SYLLSTNAER QRDASHQIRQ EARILRNETN NQIVWDEHDN RTRLAERIDT VNRWKETLDK CLTDLDAEI DSLAQAKESA EQNLQAKNLP LDVAIECLTL RESRRDIDVV RDPVEEELLK EVEVIEATKK VLQEKISQAF Q HLCLLQEI RQQLNSDHRD KMETLEIDRG CLSLNLTSPN ISLKVNPTRI PKDSTTLQQW DEFTRFNKNR AEAEMKASIE LR EAIALAI AQTNNELDAQ RVATEFTFRK RLREMESFYS ELKWQEKNTL EEIAELQGDI RRLEEDLRRK MMNLKLAHTR LES RTYRSN VELCRDQTQY GLIDEVHQLE ATINTMKQKL AQTQNALDAL FKHLARIQAD IACKTNTLLL DTKCMDTRRK LTVP AEKFV PQVDTFTRTT NRTLSPLKIC QLELT

UniProtKB: Tektin-2

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Macromolecule #6: EF-hand domain-containing family member B

MacromoleculeName: EF-hand domain-containing family member B / type: protein_or_peptide / ID: 6 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Mus musculus (house mouse)
Molecular weightTheoretical: 95.891961 KDa
SequenceString: MCSFVRVGSP KPLQTSASPL EMSSLRRTRA PEISELGLTP EQKDDIRDRV LRGSKSPTEL GFDLRLEQDR KWRERMGSSE AKSPPCHAL GVGLERHTIS GTPTEMGNLG LHKGSAFQGS KPLGVLPGRV GPENKGLPPR LRYGGTLHPP FSTVHASPLA A ESRRRPLA ...String:
MCSFVRVGSP KPLQTSASPL EMSSLRRTRA PEISELGLTP EQKDDIRDRV LRGSKSPTEL GFDLRLEQDR KWRERMGSSE AKSPPCHAL GVGLERHTIS GTPTEMGNLG LHKGSAFQGS KPLGVLPGRV GPENKGLPPR LRYGGTLHPP FSTVHASPLA A ESRRRPLA WGSAWTDAVV EKQPVVGLEL RKEPEKEPTC VVMNPYPEMP PKEVDIGLPQ TQESDEAKNT EPLIGLVREP SE CPFAQQP EEKKEPGSTE PGVEPPGNIR PIYSGKFFDR VPCWPSAGKV KPVGYRVATC LTEKLPRLMT PPEAKKYFNF RYP PAGAER VFYGRANDPQ IAPYLTHGLR SKISIPMGSL INPQPITTFQ QKIKDKKESI YFSHQRAPLG KSHDQTPGLP KGMD VINTT LGTPTIRELS VRDTVNPSKS FEDVLKEGQE GHDLYTVSHN DYFAGEAKNR KYNPASFHRF NLYGIPTPHF NDGRT MAKA LHWLHELQME RGAKIVSKRV DDFKEKFQHK LGKVLDPIAE TMNVPPGHTF GSCLHPEEYG AGDLIHYRSP DEYLRG KDH QRAVVAAARH HLKKFNHQNF DTLQVAFRHY DKKGDGVIDR AELHEACVQA NLHLDKMLLD HLFDYCDVDQ DGLINYL EF ANFLNWKDRI PLKEHEKRVV VKGKKPDCEN VTDTSMGEAE PSLLINPEDI VPKEPGSSEE TLRTIQRPGD KVSHQYKT T SSEINAVVGA VPSMCHPIFG VPTIRSDISA PRIRRVSDMN NYGDEGNAYS LLHPSIFSQK GVFERDFFKT RSKEEISDI LTNIGVKLSK EEFENVWNLA SKKHQRGEVC VETIRNVLDE LLHADLVKCK TAM

UniProtKB: EF-hand domain-containing family member B

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Macromolecule #7: Tektin-3

MacromoleculeName: Tektin-3 / type: protein_or_peptide / ID: 7 / Number of copies: 15 / Enantiomer: LEVO
Source (natural)Organism: Mus musculus (house mouse)
Molecular weightTheoretical: 56.754777 KDa
SequenceString: MELLGSTLTA TYAHPPPASA SFLPAIGTIT SSYKDRFPHR NLTHSLSLPW RPNTYYKTAY NYPTLAPYSS RSQRVCESTM LPFVSNRTT FFTRYTPDDW YRSNLVSFQE SNSSRHNSER LRVDTSRLIQ DKYQQIRKTQ AHSTQNLGER VNDLAFWKSE I THELDEMI ...String:
MELLGSTLTA TYAHPPPASA SFLPAIGTIT SSYKDRFPHR NLTHSLSLPW RPNTYYKTAY NYPTLAPYSS RSQRVCESTM LPFVSNRTT FFTRYTPDDW YRSNLVSFQE SNSSRHNSER LRVDTSRLIQ DKYQQIRKTQ AHSTQNLGER VNDLAFWKSE I THELDEMI GETNALTDIK RRLERGLIET EGPLQVSREC LFHREKRMGI DLVHDEAEKE LLAEVDTILC CQERMRQHLD KA NAQLASD RSAQHELEKD LSDKQAALRI DDKCQHLRNT SEGVSYFRGV ERVDATVSVP ETWAKFTDDN VLRSQSERAA SAK LREETE NLLIVTANEM WNQFNKVNLA FTNRIAETVD AKNKIHTHLT KTLQEIFQIE MTIESIKKAI KEKSAFLKVA QTRL DERTR RPNVELCRDM AQLRLVNEVY EVDETIQTLQ QRLRDSEDTL QSLAHTKATL EHDLAVKANT LYIDQEKCMS MRNSY PSTL RLVGYC

UniProtKB: Tektin-3

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Macromolecule #8: Meiosis-specific nuclear structural protein 1

MacromoleculeName: Meiosis-specific nuclear structural protein 1 / type: protein_or_peptide / ID: 8 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Mus musculus (house mouse)
Molecular weightTheoretical: 60.353012 KDa
SequenceString: MATKKRALSF SEKHQKLVDE KFRKSLNIQV MNKLERQAKN QVVQNENDEK VERQRFLRVL QNEQFELDME EAIQKAEANK MLRDRQLEQ EERLANELAR LKHESLKDKK MRQQVRENSI ELRELEQKLK AAYMNKERAA QIVEKDAMKY EQMKRDAEIE R IMMEEHDR ...String:
MATKKRALSF SEKHQKLVDE KFRKSLNIQV MNKLERQAKN QVVQNENDEK VERQRFLRVL QNEQFELDME EAIQKAEANK MLRDRQLEQ EERLANELAR LKHESLKDKK MRQQVRENSI ELRELEQKLK AAYMNKERAA QIVEKDAMKY EQMKRDAEIE R IMMEEHDR LLKEESAKQE RRNKERAQYY LDLEKQLEDQ ERRKQEAYEQ LLKEKLMIDE IVRKIYEEDQ VERQQKLEKK NA IQKYIEE FQRAQDFWRQ KKREEMEEEN RKIIEFANIQ EQREGERMAR VHEIEEKRVQ RQNLLMKQLE ETLRQRDDLE QVR QELYQE EQAEIIKLKV KEEAELRLRR QREMKQDFED QMALKELILQ AAKEEEETFK KAMLAKFAED DRIELMNAQK QRMK QLEHK RAVEKLIEER RSQFLADKQR ELEELQLQQR RQGCINEIIE EERLRLLKEH AAKLLGYLPK GVFKREDDVD MLGEE FRKA YQKRDGV

UniProtKB: Meiosis-specific nuclear structural protein 1

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Macromolecule #9: Tektin-4

MacromoleculeName: Tektin-4 / type: protein_or_peptide / ID: 9 / Number of copies: 8 / Enantiomer: LEVO
Source (natural)Organism: Mus musculus (house mouse)
Molecular weightTheoretical: 52.121449 KDa
SequenceString: MAQTGVLLTK EPAPQSIDVC ELPRKEYEVA CNTGAYTSSG LATAGFRTAK YLRDEWFQNS YARYHQAFAD RDYSERQRHE SGQLVAETG ALAQRTQLDS TRKVGERLED MHCWKSELQR EIDELSSETD LMMAQKLRLQ RALDATSVPY SIATDNLQCR E RRQHPDLV ...String:
MAQTGVLLTK EPAPQSIDVC ELPRKEYEVA CNTGAYTSSG LATAGFRTAK YLRDEWFQNS YARYHQAFAD RDYSERQRHE SGQLVAETG ALAQRTQLDS TRKVGERLED MHCWKSELQR EIDELSSETD LMMAQKLRLQ RALDATSVPY SIATDNLQCR E RRQHPDLV RDYVEVELLK ETELIRNIQE LLKRTIGQAV DQIRLNREHK ESCEMNWSDK VEVYNIDDTC SRYTNESTQV QF YPHSSKF EESASTPETW AKFNHDNLLR AERERLASVN LRKLIDCILR DTAEDLRLQC DAVNSAFSSR CQELDDSLQK LQY HLRKTL TEITDQEHQI AALKQAIKDK EAPLRVAQTR LYQRSHRPNV ELCRDNAQFR LLSEVEELNM SLRALKEKLQ DAEQ ALRNL EDSRMSLEKD IAVKTNSLFI DRQKCMTHRN RYPSVLQLAG YQ

UniProtKB: Tektin-4

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Macromolecule #10: Cilia- and flagella-associated protein 53

MacromoleculeName: Cilia- and flagella-associated protein 53 / type: protein_or_peptide / ID: 10 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Mus musculus (house mouse)
Molecular weightTheoretical: 62.036609 KDa
SequenceString: MYSQQFGTVP REFKGPTPKA VIIRAKPPKA QRAEQHLKRI QRSYHKYHTT LASIKSNEEN RLKCDWIQRN NHKTFDSLVQ ARVQDAMQG FVINTEERRN KLRELLASEE NEYFSEMQLK GETIEEKKDK MRERTKLLRE KKEKERQEFV AEKLDQQFRE R CEELRTKL ...String:
MYSQQFGTVP REFKGPTPKA VIIRAKPPKA QRAEQHLKRI QRSYHKYHTT LASIKSNEEN RLKCDWIQRN NHKTFDSLVQ ARVQDAMQG FVINTEERRN KLRELLASEE NEYFSEMQLK GETIEEKKDK MRERTKLLRE KKEKERQEFV AEKLDQQFRE R CEELRTKL ASIHEKKVVE ERNAQIEFNK ELKRQKLVEE HLFARLWEED RLAKERREAQ EEKRQRELVQ NTRLGLDAQV TS IQAQRQG ARRMKEEEAR ILEQNKAQIK REDEQEKLQK QKRRQETRSS LKKAVQDKIE SMQREYREDL DLNMKLVGRA LQD LQDEAD KKKQKREEMG REQKIYNDYL MQRREEEKAQ EKELNRLLED IKAKKLAEKD RELALQRAAR KQLMNEVMNT RKLQ VQERL QRKLREQEEL ALHEQRISES LKVLHQEDME DFARRCALAE EYRNQLQMQI AHQQQAREAE KEEERQEFEA GLAAN KACL DKIQRILSEN QALSQNVHPM RRGYPDKPPL

UniProtKB: Cilia- and flagella-associated protein 53

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Macromolecule #11: Tektin bundle-interacting protein 1

MacromoleculeName: Tektin bundle-interacting protein 1 / type: protein_or_peptide / ID: 11 / Number of copies: 4 / Enantiomer: LEVO
Source (natural)Organism: Mus musculus (house mouse)
Molecular weightTheoretical: 23.987262 KDa
SequenceString: MENVRREATR PSVPSGTLEL YFPDHLYRND YVSLEGPRWA PAIKQAVRWK FTPMGRDAAG QVWFTGLTNS EPGDAWYKLP RALDTPYRE AHTRWHGCFQ SRQRGLPPAY TQHLREMAFW DPAITAQYLN SGPRWGCMQW RDRQIRGKEF VVTRNQFGAK L PWRSDYVP ...String:
MENVRREATR PSVPSGTLEL YFPDHLYRND YVSLEGPRWA PAIKQAVRWK FTPMGRDAAG QVWFTGLTNS EPGDAWYKLP RALDTPYRE AHTRWHGCFQ SRQRGLPPAY TQHLREMAFW DPAITAQYLN SGPRWGCMQW RDRQIRGKEF VVTRNQFGAK L PWRSDYVP LLSLPQRPRF TAQDFRQRGL QRPCPAIGQP PPAFTPAL

UniProtKB: Tektin bundle-interacting protein 1

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Macromolecule #12: Cilia- and flagella-associated protein 141

MacromoleculeName: Cilia- and flagella-associated protein 141 / type: protein_or_peptide / ID: 12 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mus musculus (house mouse)
Molecular weightTheoretical: 12.278145 KDa
SequenceString:
MSAEKMTKLE ENLQRAVALK KTVDRWRNFH IHCMWQTTLD QRRNLFAALR MKDTKEQELA LSNKQLLVVR QAALHELFEK EYQQYQQEL NQMGKAFYEE RL

UniProtKB: Cilia- and flagella-associated protein 141

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Macromolecule #13: Nucleoside diphosphate kinase 7

MacromoleculeName: Nucleoside diphosphate kinase 7 / type: protein_or_peptide / ID: 13 / Number of copies: 2 / Enantiomer: LEVO / EC number: nucleoside-diphosphate kinase
Source (natural)Organism: Mus musculus (house mouse)
Molecular weightTheoretical: 44.489828 KDa
SequenceString: MRACQQGRSS SLVSPYMAPK NQSERFAFIA EWYDPNASLL RRYELLFYPV DGSVEMHDVK NRRTFLKRTK YEDLRLEDLF IGNKVNVFS RQLVLIDYGD QYTARQLGSR KEKTLALIKP DAVSKAGEII EMINKSGFTI TKLRMMTLTR KEAADFHVDH H SRPFYNEL ...String:
MRACQQGRSS SLVSPYMAPK NQSERFAFIA EWYDPNASLL RRYELLFYPV DGSVEMHDVK NRRTFLKRTK YEDLRLEDLF IGNKVNVFS RQLVLIDYGD QYTARQLGSR KEKTLALIKP DAVSKAGEII EMINKSGFTI TKLRMMTLTR KEAADFHVDH H SRPFYNEL IQFITSGPVI AMEILRDDAI CEWKRLLGPA NSGLSRTDAP GSIRALFGTD GVRNAAHGPD TFASAAREME LF FPSSGGC GPANTAKFTN CTCCIIKPHA ISEGMLGKNL IAIRDACFGM SAIQMFNLDR ANVEEFYEVY KGVVSEYNDM VTE LCSGPC VAIEIQQSNP TKTFREFCGP ADPEIARHLR PETLRAIFGK TKVQNAVHCT DLPEDGLLEV QYFFKILDN

UniProtKB: Nucleoside diphosphate kinase homolog 7

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Macromolecule #14: Protein FAM166B

MacromoleculeName: Protein FAM166B / type: protein_or_peptide / ID: 14 / Number of copies: 6 / Enantiomer: LEVO
Source (natural)Organism: Mus musculus (house mouse)
Molecular weightTheoretical: 30.290346 KDa
SequenceString: MAVVNTSIPG LSGENPHYIP GYTGHCPLLR FSMGQTYGQV TGQLLRGPPG LAWPPAHRTL LPPIQSPRSP VISKGRLPPR RGHERLSSS IIPGYTGFIP RAQFIFAKNC NQVWAEAMSE FTRRHGEQES HQLPDGAKGE REVEEDQLRE AEEPPLKQEL A HASPYSMD ...String:
MAVVNTSIPG LSGENPHYIP GYTGHCPLLR FSMGQTYGQV TGQLLRGPPG LAWPPAHRTL LPPIQSPRSP VISKGRLPPR RGHERLSSS IIPGYTGFIP RAQFIFAKNC NQVWAEAMSE FTRRHGEQES HQLPDGAKGE REVEEDQLRE AEEPPLKQEL A HASPYSMD DTDPHKFFMS GFTGYVPRAR FLFGSSFPVL TNQALQEFGQ MCSRGRAHKD PKPLSPLPRP TFQNLGLLPH YG GYVPGYK FQFGGTFGHL THDALGLSIT QKQLPA

UniProtKB: Ciliary microtubule inner protein 2B

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Macromolecule #15: Sperm-associated antigen 8

MacromoleculeName: Sperm-associated antigen 8 / type: protein_or_peptide / ID: 15 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mus musculus (house mouse)
Molecular weightTheoretical: 50.591805 KDa
SequenceString: METTESTEGS LSRSCDVQPS SERLDTPSEP VPSSSSSPRS TAPAEAPAQY SVLTEPSSDS LYGAPCPPAH HRGHGFGFQP FYVSCIPQD PCNMADLSSR ADPTSSYPCH SSVHGSGSGT CGLGQSSEPS QGSGPTSGPA PASVPSLVSG PDSASGPDSS A SGPALASG ...String:
METTESTEGS LSRSCDVQPS SERLDTPSEP VPSSSSSPRS TAPAEAPAQY SVLTEPSSDS LYGAPCPPAH HRGHGFGFQP FYVSCIPQD PCNMADLSSR ADPTSSYPCH SSVHGSGSGT CGLGQSSEPS QGSGPTSGPA PASVPSLVSG PDSASGPDSS A SGPALASG PGPADPGQGP KFSTCIPQGY RCIPVDLAPD YNAWCQHLHW KPQRSWEPLQ VSEPGVRGPY KPPEPGALGP CE PCEPCEP PEAESEETLC KARPRGQCLL YNWEEERATN QLDQIPPLQD GSESYFFRHG HQGLLTTQPQ SPMSSSTTQR DSY QLPRHI CQPLRGKREA MLEMLLRHQI CKEVQAEQEP ARKLFETESV THHDYRVELV RAAPPASTKP HDYRQEQPET FWIQ RAARL PGVSDIRTLD TPFRKNCSFS TPVPLSLGQP LPYELESGPH QVGVISSLAC QGGGQGCGRT KTTPI

UniProtKB: Sperm-associated antigen 8

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Macromolecule #16: RIB43A-like with coiled-coils protein 2

MacromoleculeName: RIB43A-like with coiled-coils protein 2 / type: protein_or_peptide / ID: 16 / Number of copies: 5 / Enantiomer: LEVO
Source (natural)Organism: Mus musculus (house mouse)
Molecular weightTheoretical: 45.328156 KDa
SequenceString: MEVAMSKDLQ QEANLAKKRY IDLCRQGRIF DARNRIIGGD TQAWDFQVRD QKIKEITDKA RHEAFAAEMK HNDKVMCMAH DREQRHRKQ LCRAINDFQQ NFQKPETRRE FDLSDPLALQ KELPARISDN DMRNTISGMQ KFMGEDLNFQ ERRRFQKEQS R EWFLQQHG ...String:
MEVAMSKDLQ QEANLAKKRY IDLCRQGRIF DARNRIIGGD TQAWDFQVRD QKIKEITDKA RHEAFAAEMK HNDKVMCMAH DREQRHRKQ LCRAINDFQQ NFQKPETRRE FDLSDPLALQ KELPARISDN DMRNTISGMQ KFMGEDLNFQ ERRRFQKEQS R EWFLQQHG EREKARADHL LAEHLHTQTR LKFDETAREL MKLEGSTRKE VCAAVKAFNK NQVVELTERK RQEKQQEQED NM TEITNLL HGDLLSENPR PVASSFGSHR VVLDRWKGMN REQLEEIWFT QKRQIQEKLR LQEEERQHSM DWDLRRIRKA HAS LLHERQ QQRLLREQRR ALDCSNLNLA RQQYLQKKQM NTASSSQPTE DYFSQFNTRS R

UniProtKB: RIB43A-like with coiled-coils protein 2

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Macromolecule #17: Cilia- and flagella-associated protein 107

MacromoleculeName: Cilia- and flagella-associated protein 107 / type: protein_or_peptide / ID: 17 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Mus musculus (house mouse)
Molecular weightTheoretical: 23.06251 KDa
SequenceString: MAMLSTSVVP EAFSTPGWQI EKKYSTKVLL GNWVEERGKF TKAIDHTPQC IYRKEYVPMP DHRPDFVSRW YSKSKMEGLP YKHLITHHQ EPSHRYLIST YDDHYNRHNY NPGLPALRTW NGQKLLWLPE KSDFPLVAPP TNYGLLEQLQ QKWLASKTSL K ESIYTTSY ...String:
MAMLSTSVVP EAFSTPGWQI EKKYSTKVLL GNWVEERGKF TKAIDHTPQC IYRKEYVPMP DHRPDFVSRW YSKSKMEGLP YKHLITHHQ EPSHRYLIST YDDHYNRHNY NPGLPALRTW NGQKLLWLPE KSDFPLVAPP TNYGLLEQLQ QKWLASKTSL K ESIYTTSY PRLPVCAMSR REHAIPVPHP RLQPIPRF

UniProtKB: Cilia- and flagella-associated protein 107

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Macromolecule #18: Cilia- and flagella-associated protein 161

MacromoleculeName: Cilia- and flagella-associated protein 161 / type: protein_or_peptide / ID: 18 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Mus musculus (house mouse)
Molecular weightTheoretical: 34.433383 KDa
SequenceString: MAQNVYGPGV RMGNWNEDVY LEEERMRHFL EKREKGELLI QRNRRVKKNI LRPMQLSVSE DGYVHYGDKV IIVNPDQVLG EEAGKFMRG DLSLCMSPDE VKAQLSDDLE IPCGVSAVQT IAPMGRNTFT ILSDGANSCE MGQVVVYGQN FCLGIAAGLE G KMLYLTSD ...String:
MAQNVYGPGV RMGNWNEDVY LEEERMRHFL EKREKGELLI QRNRRVKKNI LRPMQLSVSE DGYVHYGDKV IIVNPDQVLG EEAGKFMRG DLSLCMSPDE VKAQLSDDLE IPCGVSAVQT IAPMGRNTFT ILSDGANSCE MGQVVVYGQN FCLGIAAGLE G KMLYLTSD HRTLLKSSLK SGLQEVTLTD EVTHLNCWQA AFLDPQLRLE YEGFPVRANE KIVIYHRHTN RALAVHRNLF LR TYFGKEM EVVAHTYLDS HKVEKPKNQW MLVTGNPRNK SNTMLDISKP ITEDTRALEQ AMGINT

UniProtKB: Cilia- and flagella-associated protein 161

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Macromolecule #19: EF-hand domain-containing protein 1

MacromoleculeName: EF-hand domain-containing protein 1 / type: protein_or_peptide / ID: 19 / Number of copies: 3 / Enantiomer: LEVO
Source (natural)Organism: Mus musculus (house mouse)
Molecular weightTheoretical: 75.235422 KDa
SequenceString: MGTNPVHGLP FLPGSSFTDS TKTAFHRSQT LNYRNGYAVV RRPTMGIGGD RLHYNQLSQA ELDELANKAP ILTYGPLKQA PLAEFVPAH VAFDKKVLKF SAYFQEDVPI SMEEHYRIRH VNIYYYLEDD SMSVIEPVVE NSGIPQGKLI KRQRFTKNDM G DHYHWKDL ...String:
MGTNPVHGLP FLPGSSFTDS TKTAFHRSQT LNYRNGYAVV RRPTMGIGGD RLHYNQLSQA ELDELANKAP ILTYGPLKQA PLAEFVPAH VAFDKKVLKF SAYFQEDVPI SMEEHYRIRH VNIYYYLEDD SMSVIEPVVE NSGIPQGKLI KRQRFTKNDM G DHYHWKDL NRGINLTVYG KTFRIVDCDR FTQDFLESQG IELNPSEKIP LDPYTQLRKE PVRKYVTPSD FDQLKQFLTF DK QVLRFYA IWDDTDSLFG ECRHYIIHYY LMDDTVEIRE VHERNNGRDP FPLLMNRQRM PKVLVENAKN FPKCVLEISD QEV LEWYTA KDFIVGKPLT ILGRTFFIYD CDPFTRQFYK DKFGMPDLPP VDVTKKEPPP VKQELPPYNG YGLIEDSAQN CFAL IPKAP RKDVVKMLMN DNKVLRYLAA LESPIPEDKD RRFVFSYFLA TDMISIFEPP VRNSGIIGGK FLGRTKVVKS FSPVD NPIY YSPSDFFIGA VIEVFGHRFV ILDTDEYVLK YMESNASQYS PEALASIQNR IQKPELPAPE LESKQATGEP MVQGTE ESK VQDLDALIDQ IHMHLKYNSY KENLRETFQM YDKDESGYVD RETFFKICET LNVPVDDSLI KELIRLCTHG EGRINYY NF VRAFSN

UniProtKB: EF-hand domain-containing protein 1

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Macromolecule #20: Sperm acrosome-associated protein 9

MacromoleculeName: Sperm acrosome-associated protein 9 / type: protein_or_peptide / ID: 20 / Number of copies: 27 / Enantiomer: LEVO
Source (natural)Organism: Mus musculus (house mouse)
Molecular weightTheoretical: 19.512373 KDa
SequenceString:
MNEVKESLRS IEQKYKLFQQ QQFTFIAALE HCRENAHDKI RPISSIEQVQ SYMEHYCNNS THRRILIMFM DICSELSKLC QHFEALHSG TPVTNSLLEK CKTLVSQSND LSSLRAKYPH EVVNHLSCDE ARNHYGGVVS LIPIVLDFMK EWIAHSEKLP R KVLQHGTT

UniProtKB: Sperm acrosome-associated protein 9

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Macromolecule #21: EF-hand domain-containing family member C2

MacromoleculeName: EF-hand domain-containing family member C2 / type: protein_or_peptide / ID: 21 / Number of copies: 4 / Enantiomer: LEVO
Source (natural)Organism: Mus musculus (house mouse)
Molecular weightTheoretical: 87.758023 KDa
SequenceString: MALPFLPGNS FNRNIGKERF HKSQHWGFCN NVRMLVSENK PGVGGDLLYG QKIKPKHSVF PKGDGTDAPS WVAFDKQVLS FDAYLEDEI SDKRQEIFRI RYYKIYFYLE DDTIQVNEPE VINSGLPQGT SIRRQRIPYP PPNDDQFYTV YDFNINISVV F YGRTFKIY ...String:
MALPFLPGNS FNRNIGKERF HKSQHWGFCN NVRMLVSENK PGVGGDLLYG QKIKPKHSVF PKGDGTDAPS WVAFDKQVLS FDAYLEDEI SDKRQEIFRI RYYKIYFYLE DDTIQVNEPE VINSGLPQGT SIRRQRIPYP PPNDDQFYTV YDFNINISVV F YGRTFKIY DCDPFTKNFL KKIGIKLNPP GQCPLDPYMK MRRETLEFVD PFRPYQSFDT LKRFIQYDGK VLRFFCLWDD ST SLFGDRR EFVLHYFLCD GTVEIREVLP SNSGRDAMSS FLRRGKLPKY GPPGIYQPGQ ITDRAVLNVY GGLSEWRADG YLL DKYQLG KVEQDFYTDQ DLSIGATINV WGRKVLLCDC DEFTKTYYRT KYGVDNFTPI SCKPPHLPKI ERKYPPYTGF GSEE DSFRS CVGLKPTPHR KNFKKFMELD SFGNISNILR YFGKLITHKC ADVDRIFVIA FYLSDDTISV FEPIENNSGN AGGMF LKRS RVKKPGQEVF KSEFSEYIKA EELYIGATVN INGYLFILLN ADEYTLNYME NNTDKFPYSN FELAIQKLKQ EKSKSR EIT QVFAAADYNH TKVVPYNTFR DILMSITMGK LIDQELITIA RHYRVPEIMD PDLAYLIARA HEKFKKNIFE NFDMFIY NC VYEDREKKGV LPTKDIRRMC KSSRLPLDDD FLDCLLSRFE DKDHQINYEI FFSVLNWRMN PTPDLQAPPY LKEKCEDV W VGMPSPIPVK YVRYLDFLID VYGLEDNML

UniProtKB: EF-hand domain-containing family member C2

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Macromolecule #22: Cilia- and flagella-associated protein 20

MacromoleculeName: Cilia- and flagella-associated protein 20 / type: protein_or_peptide / ID: 22 / Number of copies: 7 / Enantiomer: LEVO
Source (natural)Organism: Mus musculus (house mouse)
Molecular weightTheoretical: 22.781389 KDa
SequenceString: MFKNTFQSGF LSILYSIGSK PLQIWDKKVR NGHIKRITDN DIQSLVLEIE GTNVSTTYIT CPADPKKTLG IKLPFLVMII KNLKKYFTF EVQVLDDKNV RRRFRASNYQ STTRVKPFIC TMPMRLDDGW NQIQFNLSDF TRRAYGTNYI ETLRVQIHAN C RIRRVYFS ...String:
MFKNTFQSGF LSILYSIGSK PLQIWDKKVR NGHIKRITDN DIQSLVLEIE GTNVSTTYIT CPADPKKTLG IKLPFLVMII KNLKKYFTF EVQVLDDKNV RRRFRASNYQ STTRVKPFIC TMPMRLDDGW NQIQFNLSDF TRRAYGTNYI ETLRVQIHAN C RIRRVYFS DRLYSEDELP AEFKLYLPVQ NKAKQ

UniProtKB: Cilia- and flagella-associated protein 20

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Macromolecule #23: Parkin coregulated gene protein homolog

MacromoleculeName: Parkin coregulated gene protein homolog / type: protein_or_peptide / ID: 23 / Number of copies: 5 / Enantiomer: LEVO
Source (natural)Organism: Mus musculus (house mouse)
Molecular weightTheoretical: 27.763268 KDa
SequenceString: MPKRTKLLPQ QTFQVHQPRS LVSEGFTVKA MMKNSVVRGP PVAGAFKERP AKPTTFRKCY ERGDFPIALE HDSKGNKIAW KVEIEKLDY HHYLPLFFDG LSEMTFPYEF FARRGIHDML EHGGNKILPV IPQLIIPIKN ALNLRNRQII CVTLKVLQHL V VSSEMVGE ...String:
MPKRTKLLPQ QTFQVHQPRS LVSEGFTVKA MMKNSVVRGP PVAGAFKERP AKPTTFRKCY ERGDFPIALE HDSKGNKIAW KVEIEKLDY HHYLPLFFDG LSEMTFPYEF FARRGIHDML EHGGNKILPV IPQLIIPIKN ALNLRNRQII CVTLKVLQHL V VSSEMVGE ALLPYYRQIL PILNIFKNMN VNSGDGIDYS QQKRENIGDL IQETLEAFER YGGEDAFINI KYMVPTYESC LL N

UniProtKB: Parkin coregulated gene protein homolog

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Macromolecule #24: Dual specificity protein phosphatase 3

MacromoleculeName: Dual specificity protein phosphatase 3 / type: protein_or_peptide / ID: 24 / Number of copies: 3 / Enantiomer: LEVO / EC number: protein-serine/threonine phosphatase
Source (natural)Organism: Mus musculus (house mouse)
Molecular weightTheoretical: 20.494127 KDa
SequenceString:
MSSSFELSVQ DLNDLLSDGS GCYSLPSQPC NEVVPRVYVG NASVAQDITQ LQKLGITHVL NAAEGRSFMH VNTSASFYED SGITYLGIK ANDTQEFNLS AYFERATDFI DQALAHKNGR VLVHCREGYS RSPTLVIAYL MMRQKMDVKS ALSTVRQNRE I GPNDGFLA QLCQLNDRLA KEGKVKL

UniProtKB: Dual specificity protein phosphatase 3

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Macromolecule #25: Piercer of microtubule wall 2 protein

MacromoleculeName: Piercer of microtubule wall 2 protein / type: protein_or_peptide / ID: 25 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mus musculus (house mouse)
Molecular weightTheoretical: 13.728513 KDa
SequenceString:
MARETDCDLD KKTSLTSDAE MRPEPPALCV NPGNPVFSCM LDPKTLHTAT SLSKAQMIMY KTSASQYGAF SPRPFFFPCK FLPQEQAFT EHLKTTGFYQ NNSLNVGPDR TRTIDSPNYQ HTL

UniProtKB: Piercer of microtubule wall 2 protein

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Macromolecule #26: Tektin-5

MacromoleculeName: Tektin-5 / type: protein_or_peptide / ID: 26 / Number of copies: 23 / Enantiomer: LEVO
Source (natural)Organism: Mus musculus (house mouse)
Molecular weightTheoretical: 62.817535 KDa
SequenceString: MEFLGTTQTA SFCGPKKGCG LQALPPAGQE PVVQECYQPF HLPGYRYLNA WRPSVFHKIA TSQTIPEECS GIRRPPTILP SLRSALFCR YTPRDWDRSN DLQIRNAEAS RLWASRLTGD SLRIMQDKDQ LIHQMQEGTS RNLGQRLSDL GFWKSELCYE L DRLLTENS ...String:
MEFLGTTQTA SFCGPKKGCG LQALPPAGQE PVVQECYQPF HLPGYRYLNA WRPSVFHKIA TSQTIPEECS GIRRPPTILP SLRSALFCR YTPRDWDRSN DLQIRNAEAS RLWASRLTGD SLRIMQDKDQ LIHQMQEGTS RNLGQRLSDL GFWKSELCYE L DRLLTENS SMDTLKRRLE CAAEEVNCPL QVALECLYNR EKRIGIDLVH DNVEKNLIRE VDLLKCCQDQ MRKLAKRIDF QI RDNRDAQ HSLERDIEDK SSAQYIDENC FNLRSTSDSI SFFHGVEKFD GTVSIPETWA KFSNDNIRHA QNMRANSIRL REE AEHLFE TLSDQMWKQF TNTNLAFNAR ISEETDVKNK LQTQLAKILQ EIFQAENTIM LLERAIVAKE YPLKMAQTML ACRT RRPNV ELCRDVPQFR LVNEVFTIDD TLQTLKLRLR ETQDTLQLLV MTKSRLEHEL AIKANTLCID KDKCMSMRKS FPSTP RLTG YTCSAIGSGP YANHAPRISS GPCSGSALCK GPASCGGGAS CGGGASCGGH APCGSALCSH SVSRSGPGFA PVC

UniProtKB: Tektin-5

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Macromolecule #27: Cilia- and flagella-associated protein 45

MacromoleculeName: Cilia- and flagella-associated protein 45 / type: protein_or_peptide / ID: 27 / Number of copies: 5 / Enantiomer: LEVO
Source (natural)Organism: Mus musculus (house mouse)
Molecular weightTheoretical: 65.962016 KDa
SequenceString: MPLRPGDASS SASTASNRSR TRTRYRTKAM NSEVDESLFG GVKPSSQGKS DSPIVVIHDK HAIRKTLSAL GLEHKTETIQ LITRDMVRE LIVPTEDPSG ESLIISPEEF ERIKWASQVL TKEELNAREQ ALKKEKEGIL EAVTIRKKIM KQKEMTWNNN K KLSDLEEV ...String:
MPLRPGDASS SASTASNRSR TRTRYRTKAM NSEVDESLFG GVKPSSQGKS DSPIVVIHDK HAIRKTLSAL GLEHKTETIQ LITRDMVRE LIVPTEDPSG ESLIISPEEF ERIKWASQVL TKEELNAREQ ALKKEKEGIL EAVTIRKKIM KQKEMTWNNN K KLSDLEEV ARERAQNLLQ RADKLRMEQE EELKDMSKII LNAKCHAIRD AQILEKQQIQ KELDEEERRL DHMMEIDRRE SL QRQEDRE RKRREERVRG KRHIVEQIKK NEEERSLQAE HREQEKEQML AYLDRLQEED LQDLERRHQE KLKMQAEIKR IND ENQRQK AEMLAQERLA DQMVMEFTKK KMAREAEYEA EQEKIRREKE KEIARLRALQ EKAQDYQAEQ DALRAKRNQE VADR EWRRK EKENAQKKIE TEEKLRKSRL EQVAFKEHTL AVQVQRDRDE FERILRAQRE QIEREKQEQE KKAKGCLQHA NELRR QVRE NQQKHVQNRL ATFEEGRRLK EEAEKRRERI EDIKKQKLEE LRATGLPEKY CIEVERKANI LPATSVN

UniProtKB: Cilia- and flagella-associated protein 45

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Macromolecule #28: Cilia- and flagella-associated protein 52

MacromoleculeName: Cilia- and flagella-associated protein 52 / type: protein_or_peptide / ID: 28 / Number of copies: 3 / Enantiomer: LEVO
Source (natural)Organism: Mus musculus (house mouse)
Molecular weightTheoretical: 68.322164 KDa
SequenceString: MEEQVLPELD VAELELQAVI GFNGHVPNGL KCHPDQEHLI YPLGCTVLIQ AINTNEQNFL HGHGNNVSCV TISKEGDYIA SGQVTFMGF KADIILWDFK KRELIARLSL HKGKIEALAF SPNDLYLVSL GGPDDGSVVV WSIAKRDAIC GSPAAGLNVG N ATSVVFSR ...String:
MEEQVLPELD VAELELQAVI GFNGHVPNGL KCHPDQEHLI YPLGCTVLIQ AINTNEQNFL HGHGNNVSCV TISKEGDYIA SGQVTFMGF KADIILWDFK KRELIARLSL HKGKIEALAF SPNDLYLVSL GGPDDGSVVV WSIAKRDAIC GSPAAGLNVG N ATSVVFSR CRDEMFVTAG NGTIRVWELD LPNRKIWPTE CQTGQMKRIV LSTGMADDDS FFYLGTTTGD ILKMNPKTKL LA DTGPVKD KFSLGVSALR CLKMGGLLVG SGAGLLIFCK SPSYKPIKKV QLQGGITSIT LRGEGHQFFV GTEESHIYRV NFT DFKETL IATCHFEAVQ DIVFPFGTAE LFATCAKKDI RVWHTMSKRE LLRITVPNMT CHGIDFMRDG KSIISAWDDG KIRA FAPES GRLMYTINSA HRIGVTAIAT TSDCKRIISG GGEGEVRVWQ VGCQTQKLEE ALKEHKSSVS CIRVKKNNEE CVTAS TDGT CIIWDLVRLR RNQMILANTL FQCVCYHPEE FQIITSGTDR KIAYWEVFDG SVIRELEGSL SGSINGMDIT QEGGHF VTG GHDHLVKVWD YNEGEVTHVG VGHSGNIMAM RISPGNQYIV SVSADGAILR WKYPFAS

UniProtKB: Cilia- and flagella-associated protein 52

+
Macromolecule #29: Enkurin

MacromoleculeName: Enkurin / type: protein_or_peptide / ID: 29 / Number of copies: 4 / Enantiomer: LEVO
Source (natural)Organism: Mus musculus (house mouse)
Molecular weightTheoretical: 29.587521 KDa
SequenceString: MDSPCTSESI YNLIPSDLKE PPQHPRYTSL FRATIKNDMK KFKTAMKTMG PAKVEIPSPK DFLKKHSKEK TLPPKKKFNR CSPKKPAVP LRTDHPVMGI QSGKNFINTN AADVIMGVAK KPKPIYVDKR TGDKHDLETS GLFPKYINKK DYGITPEYIC K RNEDVKKA ...String:
MDSPCTSESI YNLIPSDLKE PPQHPRYTSL FRATIKNDMK KFKTAMKTMG PAKVEIPSPK DFLKKHSKEK TLPPKKKFNR CSPKKPAVP LRTDHPVMGI QSGKNFINTN AADVIMGVAK KPKPIYVDKR TGDKHDLETS GLFPKYINKK DYGITPEYIC K RNEDVKKA QEEYDNYIQE NLKKAAMKRL SDEEREAVLQ GLKKNWEEVH KEFQSLSVFI DSVPKKIRKQ KLEKEMKQLE HD ISVIEKH KIIYIANK

UniProtKB: Enkurin

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Macromolecule #30: Protein Flattop

MacromoleculeName: Protein Flattop / type: protein_or_peptide / ID: 30 / Number of copies: 3 / Enantiomer: LEVO
Source (natural)Organism: Mus musculus (house mouse)
Molecular weightTheoretical: 20.575123 KDa
SequenceString:
MATNYSANQY EKAYLPTYLQ NWSPARPTKE KIAAHEGYTQ IIANDRGHLL PSVPRSKASP WGSFMGTWQM PLKIPPAKVT LTARTTTAA DNLTKWIHKN PDLLNACNGL RPEISGKPFD PDSQTKQKKS VTKTVQQAPN PTIIPSSPVI QGDNPDEPQS S HPSAGHTP GPQTPVNSPN NPPPSPCKST K

UniProtKB: Protein Flattop

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Macromolecule #31: Cilia- and flagella- associated protein 210

MacromoleculeName: Cilia- and flagella- associated protein 210 / type: protein_or_peptide / ID: 31 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Mus musculus (house mouse)
Molecular weightTheoretical: 65.26652 KDa
SequenceString: MQVRFGRRSG QAKESQGMDC FEKEEIPYPP LLPSKVDLQQ ITIIPHAEWE RIRDSLNRLT REAAVLRAER AAKKKMHVKS QELVKHWTN TYAGMKEQKL KAKQKRDEEI EAERKVLDLE EEIYKEGERK KAIESARQCQ FHQTERVKRF HSGLLLSRVM K ERDVQIQY ...String:
MQVRFGRRSG QAKESQGMDC FEKEEIPYPP LLPSKVDLQQ ITIIPHAEWE RIRDSLNRLT REAAVLRAER AAKKKMHVKS QELVKHWTN TYAGMKEQKL KAKQKRDEEI EAERKVLDLE EEIYKEGERK KAIESARQCQ FHQTERVKRF HSGLLLSRVM K ERDVQIQY KKNAVKSDKK WEEQVKLNDE KAFKEDQEKE EKRRRERVAL AEDHLKQIEE HKEEEEARKK SEEKDAEEMK RQ NLLYEIE MKKNLSKKQE EIDTNRKLLL DNMHNKNIIR AVEQQQQEEE DEKIRKFIKA KKRLIQMRMD KDAETHRLME ERR ERINNF LSKLIKEKLD TEDLIIARDI SEADAELEKR EKEKHEKNQA DLKAIAEYRA SVMKNKEEEE RQRKIEAKEQ LQAV LKADK IFQELEKEKS LKVTREKLEI QDAHIQQIAI NKYNAKQMKE EELDYWRLTD ALTVEKEKEF EKYAREVINF ESEST KKYA YPMVKAVQEG VGGGRGPPFV GRGGIRPSYQ ATDATGVQLP CFKSQGSKYN DFQKSKRRLG FTW

UniProtKB: Cilia- and flagella- associated protein 210

+
Macromolecule #32: Cilia- and flagella-associated protein 276

MacromoleculeName: Cilia- and flagella-associated protein 276 / type: protein_or_peptide / ID: 32 / Number of copies: 3 / Enantiomer: LEVO
Source (natural)Organism: Mus musculus (house mouse)
Molecular weightTheoretical: 18.960092 KDa
SequenceString:
MLQPQETFSN PALRDEDKHL GNLWASKKSL YKNPAHLAQQ QDPWSRLSST PTATSRSRDT FFDSKIPKDD LDFRLATLYN HHTGAFKNK TEILLHQETI EDIQGTKIQF PGECFHAPSA PITSRTTIRH WINPKKESIH SIQGSIVSPH TAATNGGYSR K NDGGFFST

UniProtKB: Cilia- and flagella-associated protein 276

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Macromolecule #33: EF-hand calcium-binding domain-containing protein 6

MacromoleculeName: EF-hand calcium-binding domain-containing protein 6 / type: protein_or_peptide / ID: 33 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mus musculus (house mouse)
Molecular weightTheoretical: 176.028312 KDa
SequenceString: MKRNGTRLNF AKANSTKSGS TRARDLMNKM SIIPEWHSLY SNTRKLPCSR PHSSPCKMQR TIFQDLCRPS SSTTAIANPV LSYLDIERI LAQKISSRRD DIKKVFQILD RNHNQMVTKG DLKRVITAFL IPLTKDQFQD LLAQIPISSL GNVPYLEFLS R FGGGIDIN ...String:
MKRNGTRLNF AKANSTKSGS TRARDLMNKM SIIPEWHSLY SNTRKLPCSR PHSSPCKMQR TIFQDLCRPS SSTTAIANPV LSYLDIERI LAQKISSRRD DIKKVFQILD RNHNQMVTKG DLKRVITAFL IPLTKDQFQD LLAQIPISSL GNVPYLEFLS R FGGGIDIN INGIKRVNEN EVDNRRTVKE VQLTEKIFRN MRSIRKVFQV MDVNNTGLVQ PQELRRVLET FCLRMQDGDY EK FLEQYNI DKTTAVDYNA FLKNLSVKND ASFKYLLSNA AELSRETQQG KNGKRLLDTG SSEDVWKNYS LDDLEKTFCQ EFS KSYEKI EKALSAGDPS KGGYISLNYL KVVLDTFIYR LPRRIFIQLI RRFGLKTSTK INWKQFLTAI YERQKLEVSK TLPL KKRSS TEARNRSRKE NIIKKLFKYS EDRYTALKKT LLIISSTPSG HITWEELRHI LNCMVAKLND SEFSELKQTF DPEGT GAVR VNSLLDVLDD STKVRKMSPS TDTKTPLPVA WDSVEELVLD SITRNLQAFY SMLQSYDLRD TGTIGKNNFR KVMRVF CPY LSNEHLVRFS SKFQEAGSGR ILYKKFLLSI GVGIPPPTPP LSSPKVQLSD QFQIEEPGQQ DERTQPSGEK TSEINNM TK EEVIDGLKHR IQQKDPVFRK QFLSISKEPD VKINQEEFRK VLERSGMPMN DCQYAMLASK LGFKNEEGMS YQDFTMGF E DCMLSGLETV PLQSRTASRT NMDEHFISAE ECLRIFPKKL KESFRDVYSA FFRIDLDRDG IISMHDFHRL LQYLQLNMV DLEFERFLSL LGLRLSVTLN FREFQNLCEK RPWKSDEAPQ RLIRCKQKVA DSELACEQAH QYLIMKAKTR WADLSKNFIE TDNEGNGIL RRRDIKNSLY GFDIPLTPRE FEKLWQNYDT EGRGYITYQE FLHRLGIRYS PKVHRPYKED YFNFLGHFTK P KQVQEEIQ ELQQISEREK LMNHYEEISK AFNAMEKSKP VALCRVQKVL QECGCPLKEE ELISLLKSLD VSVHNNHIDP VE FLRALEI SWASKARPKE KEESSPPPIS FSKVTPDEVI KTMQEVVESS QPALVEAFSA LDKEDTGFVK AMEFGDVLRS VCQ KLTDNQ YHYFLRRLRL HLTPNIHWKY FLENFSTFQD ETADDWAENM PKAPPPMSPK ETAHRDIVAR VQKAVASHYH TIVQ EFENF DTLKSNTVSR DEFRSICTRH IQILTDEQFD RLWSELPVNA KGRLKYQDFL SKLSIERVPS PPMAAGDSGE STMAQ RGSS APEFSQGTRS NLYSPPRDSR VGLKSRSHPC TPVGTPPLQN CEPIESRLRK QIQGCWRELL RECKEKDTDK QGTISA AEF LALVEKFKLD ISREESQQLI VKYDLKNNGK FAYCDFIQSC VLLLKAKETS LMRRMRIQNA DKMKEAGMET PSFYSAL LR IQPKIVHCWR PMRRSFKTYD KNGTGLLSVA DFRKVLRQYS INLSEEEFFH VLEYYDKSLS SKISYNDFLR AFLQ

UniProtKB: EF-hand calcium-binding domain-containing protein 6

+
Macromolecule #34: Coiled-coil domain-containing protein 105

MacromoleculeName: Coiled-coil domain-containing protein 105 / type: protein_or_peptide / ID: 34 / Number of copies: 5 / Enantiomer: LEVO
Source (natural)Organism: Mus musculus (house mouse)
Molecular weightTheoretical: 57.406484 KDa
SequenceString: MPVLLPSTDP GQDSRVGAPE WRQAAKATSR KAHLLTDRCG QEAVTMWQPK DSVLDPNVAH HLGRAAYMEP WRFRVEMLKG GGTLEKPPP GEGVTLWKGK MKPPAWYARL PLPMHRDARA QQTAEVVHAH ARGARLTAAR LGRAQHQING QLRLLLRQRE A TDRRLSEV ...String:
MPVLLPSTDP GQDSRVGAPE WRQAAKATSR KAHLLTDRCG QEAVTMWQPK DSVLDPNVAH HLGRAAYMEP WRFRVEMLKG GGTLEKPPP GEGVTLWKGK MKPPAWYARL PLPMHRDARA QQTAEVVHAH ARGARLTAAR LGRAQHQING QLRLLLRQRE A TDRRLSEV RKGLLINQQS VKLRGYRPKC EKIPDKADSL LVWEKKELKS MKRKMEKDME ISEDLLKALA SCRDTLDFYC QE RLQAVEL MNQPLDKVLE QAGRHSWVDI TRPPTPRTQG LKTPPPDPIG TYTPACAKAL FEAKRLLMES KDILTEMAKN EVD IQNQQQ EISNRVCSSL AQKMRETLEL KERMTMTLGL MRGTIHRCMK FNQEMYVTRG IIKGPLLKRN LEAREKLNRP LVRM YQRHV GTQLPEATRL AQGTDLLTRH NLHMEKNLKE LRTTHDNLAW GLNCKKIGHD VDYDVVRLRL RQRHPHVCYE QAQRL VNDW DPPTPARSQT NTASK

UniProtKB: Tektin-like protein 1

+
Macromolecule #35: Piercer of microtubule wall 1 protein

MacromoleculeName: Piercer of microtubule wall 1 protein / type: protein_or_peptide / ID: 35 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mus musculus (house mouse)
Molecular weightTheoretical: 18.862852 KDa
SequenceString:
MSEEKPQQSA EEPEPGEPKA KPAPEEPEPG EPKAKPAPEE PEPGEPKAKP APEKTSDYYR ISEKLPVRFN NPGWFHGYGT KEAVSMYRT SNQTYGSRAP TVHEMPKVFY PSSNKFSRQH AAFGMFQSHN INVTLEKSLV TGPDNHITHY DRLNFHPSYN V NRPSICD

UniProtKB: Piercer of microtubule wall 1 protein

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Experimental details

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Structure determination

Methodcryo EM
Processingsubtomogram averaging
Aggregation statecell

-
Sample preparation

BufferpH: 7.4
VitrificationCryogen name: ETHANE

-
Electron microscopy

MicroscopeFEI TITAN KRIOS
Image recordingFilm or detector model: GATAN K3 BIOQUANTUM (6k x 4k) / Average electron dose: 4.0 e/Å2
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Nominal defocus max: 6.0 µm / Nominal defocus min: 2.0 µm
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

-
Image processing

Final reconstructionApplied symmetry - Point group: C1 (asymmetric) / Resolution.type: BY AUTHOR / Resolution: 7.7 Å / Resolution method: FSC 0.143 CUT-OFF / Software - Name: RELION (ver. 4.0-beta2) / Number subtomograms used: 12848
ExtractionNumber tomograms: 76 / Number images used: 32288
Details: 32288 particles were initially picked every 24 nm along the microtubules.
Final angle assignmentType: MAXIMUM LIKELIHOOD

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