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Yorodumi- EMDB-40753: The 2alpha3beta stoichiometry of full-length human alpha4beta2 ni... -
+Open data
-Basic information
Entry | Database: EMDB / ID: EMD-40753 | ||||||||||||
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Title | The 2alpha3beta stoichiometry of full-length human alpha4beta2 nicotinic acetylcholine receptor in complex with acetylcholine | ||||||||||||
Map data | |||||||||||||
Sample |
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Keywords | cys-loop ligand-gated pentameric ion channels / cation-selective channel / acetylcholine / TRANSPORT PROTEIN | ||||||||||||
Function / homology | Function and homology information vestibulocochlear nerve development / lateral geniculate nucleus development / regulation of circadian sleep/wake cycle, REM sleep / regulation of synaptic transmission, dopaminergic / quaternary ammonium group binding / synaptic transmission involved in micturition / Highly sodium permeable postsynaptic acetylcholine nicotinic receptors / Highly calcium permeable nicotinic acetylcholine receptors / optic nerve morphogenesis / response to acetylcholine ...vestibulocochlear nerve development / lateral geniculate nucleus development / regulation of circadian sleep/wake cycle, REM sleep / regulation of synaptic transmission, dopaminergic / quaternary ammonium group binding / synaptic transmission involved in micturition / Highly sodium permeable postsynaptic acetylcholine nicotinic receptors / Highly calcium permeable nicotinic acetylcholine receptors / optic nerve morphogenesis / response to acetylcholine / Highly calcium permeable postsynaptic nicotinic acetylcholine receptors / central nervous system projection neuron axonogenesis / acetylcholine-gated channel complex / cholinergic synapse / regulation of dopamine metabolic process / negative regulation of action potential / behavioral response to nicotine / positive regulation of dopamine secretion / acetylcholine receptor activity / inhibitory postsynaptic potential / synaptic transmission, cholinergic / acetylcholine binding / postsynaptic specialization membrane / nervous system process / acetylcholine receptor signaling pathway / acetylcholine-gated monoatomic cation-selective channel activity / regulation of synapse assembly / action potential / regulation of dendrite morphogenesis / heterocyclic compound binding / B cell activation / regulation of dopamine secretion / plasma membrane raft / membrane depolarization / associative learning / social behavior / ligand-gated monoatomic ion channel activity / smooth muscle contraction / monoatomic ion transport / positive regulation of B cell proliferation / sensory perception of pain / visual perception / response to cocaine / learning / locomotory behavior / regulation of membrane potential / sensory perception of sound / response to nicotine / visual learning / memory / cognition / calcium ion transport / presynaptic membrane / chemical synaptic transmission / postsynaptic membrane / response to ethanol / response to oxidative stress / response to hypoxia / neuron projection / external side of plasma membrane / DNA repair / neuronal cell body / synapse / dendrite / protein-containing complex binding / signal transduction / membrane / plasma membrane Similarity search - Function | ||||||||||||
Biological species | Homo sapiens (human) / Mus musculus (house mouse) | ||||||||||||
Method | single particle reconstruction / cryo EM / Resolution: 2.4 Å | ||||||||||||
Authors | Kang G / Hibbs RE | ||||||||||||
Funding support | United States, 3 items
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Citation | Journal: To Be Published Title: Structure of the 2alpha3beta stoichiometry of the full-length human alpha4beta2 nicotinic receptor in complex with acetylcholine Authors: Kang G / Mazzaferro S / Natarajan K / Sine S / Hibbs RE | ||||||||||||
History |
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-Structure visualization
Supplemental images |
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-Downloads & links
-EMDB archive
Map data | emd_40753.map.gz | 11.1 MB | EMDB map data format | |
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Header (meta data) | emd-40753-v30.xml emd-40753.xml | 21.2 KB 21.2 KB | Display Display | EMDB header |
FSC (resolution estimation) | emd_40753_fsc.xml | 10.6 KB | Display | FSC data file |
Images | emd_40753.png | 442.3 KB | ||
Filedesc metadata | emd-40753.cif.gz | 7.1 KB | ||
Others | emd_40753_half_map_1.map.gz emd_40753_half_map_2.map.gz | 80.5 MB 80.5 MB | ||
Archive directory | http://ftp.pdbj.org/pub/emdb/structures/EMD-40753 ftp://ftp.pdbj.org/pub/emdb/structures/EMD-40753 | HTTPS FTP |
-Validation report
Summary document | emd_40753_validation.pdf.gz | 761.8 KB | Display | EMDB validaton report |
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Full document | emd_40753_full_validation.pdf.gz | 761.3 KB | Display | |
Data in XML | emd_40753_validation.xml.gz | 17.9 KB | Display | |
Data in CIF | emd_40753_validation.cif.gz | 23.6 KB | Display | |
Arichive directory | https://ftp.pdbj.org/pub/emdb/validation_reports/EMD-40753 ftp://ftp.pdbj.org/pub/emdb/validation_reports/EMD-40753 | HTTPS FTP |
-Related structure data
Related structure data | 8st0MC M: atomic model generated by this map C: citing same article (ref.) |
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Similar structure data | Similarity search - Function & homologyF&H Search |
-Links
EMDB pages | EMDB (EBI/PDBe) / EMDataResource |
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Related items in Molecule of the Month |
-Map
File | Download / File: emd_40753.map.gz / Format: CCP4 / Size: 103 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) | ||||||||||||||||||||
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Voxel size | X=Y=Z: 1.079 Å | ||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||
Details | EMDB XML:
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-Supplemental data
-Half map: #1
File | emd_40753_half_map_1.map | ||||||||||||
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Projections & Slices |
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Density Histograms |
-Half map: #2
File | emd_40753_half_map_2.map | ||||||||||||
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Projections & Slices |
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Density Histograms |
-Sample components
+Entire : A complex of three Fab fragments with the 2alpha3beta stoichiomet...
+Supramolecule #1: A complex of three Fab fragments with the 2alpha3beta stoichiomet...
+Macromolecule #1: Neuronal acetylcholine receptor subunit alpha-4
+Macromolecule #2: Neuronal acetylcholine receptor subunit beta-2
+Macromolecule #3: IgG1 Kappa Light Chain
+Macromolecule #4: IgG1 Heavy Chain
+Macromolecule #6: 2-acetamido-2-deoxy-beta-D-glucopyranose
+Macromolecule #7: ACETYLCHOLINE
+Macromolecule #8: N-OCTANE
+Macromolecule #9: SODIUM ION
+Macromolecule #10: water
-Experimental details
-Structure determination
Method | cryo EM |
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Processing | single particle reconstruction |
Aggregation state | particle |
-Sample preparation
Buffer | pH: 7.4 |
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Grid | Model: Quantifoil R1.2/1.3 / Material: COPPER / Pretreatment - Type: GLOW DISCHARGE |
Vitrification | Cryogen name: ETHANE / Chamber humidity: 100 % / Instrument: FEI VITROBOT MARK IV |
-Electron microscopy
Microscope | FEI TITAN KRIOS |
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Image recording | Film or detector model: GATAN K3 (6k x 4k) / Average electron dose: 50.0 e/Å2 |
Electron beam | Acceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN |
Electron optics | Illumination mode: OTHER / Imaging mode: BRIGHT FIELD / Nominal defocus max: 2.0 µm / Nominal defocus min: 0.8 µm |
Sample stage | Specimen holder model: FEI TITAN KRIOS AUTOGRID HOLDER |
Experimental equipment | Model: Titan Krios / Image courtesy: FEI Company |