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基本情報
登録情報 | ![]() | |||||||||
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タイトル | Structure of PSII-FCPII-I/J/K complex in the PSII-FCPII supercomplex from Cyclotella meneghiniana | |||||||||
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![]() | PSII-FCPII / PHOTOSYNTHESIS | |||||||||
生物種 | ![]() | |||||||||
手法 | 単粒子再構成法 / クライオ電子顕微鏡法 / 解像度: 3.48 Å | |||||||||
![]() | Shen LL / Li ZH / Shen JR / Wang WD | |||||||||
資金援助 | ![]()
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![]() | ![]() タイトル: Structural insights into photosystem II supercomplex and trimeric FCP antennae of a centric diatom Cyclotella meneghiniana. 著者: Songhao Zhao / Lili Shen / Xiaoyi Li / Qiushuang Tao / Zhenhua Li / Caizhe Xu / Cuicui Zhou / Yanyan Yang / Min Sang / Guangye Han / Long-Jiang Yu / Tingyun Kuang / Jian-Ren Shen / Wenda Wang / ![]() ![]() 要旨: Diatoms are dominant marine algae and contribute around a quarter of global primary productivity, the success of which is largely attributed to their photosynthetic capacity aided by specific ...Diatoms are dominant marine algae and contribute around a quarter of global primary productivity, the success of which is largely attributed to their photosynthetic capacity aided by specific fucoxanthin chlorophyll-binding proteins (FCPs) to enhance the blue-green light absorption under water. We purified a photosystem II (PSII)-FCPII supercomplex and a trimeric FCP from Cyclotella meneghiniana (Cm) and solved their structures by cryo-electron microscopy (cryo-EM). The structures reveal detailed organizations of monomeric, dimeric and trimeric FCP antennae, as well as distinct assemblies of Lhcx6_1 and dimeric FCPII-H in PSII core. Each Cm-PSII-FCPII monomer contains an Lhcx6_1, an FCP heterodimer and other three FCP monomers, which form an efficient pigment network for harvesting energy. More diadinoxanthins and diatoxanthins are found in FCPs, which may function to quench excess energy. The trimeric FCP contains more chlorophylls c and fucoxanthins. These diversified FCPs and PSII-FCPII provide a structural basis for efficient light energy harvesting, transfer, and dissipation in C. meneghiniana. | |||||||||
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その他 | ![]() ![]() | 474.3 MB 474.3 MB | ||
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-検証レポート
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マップ
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ボクセルのサイズ | X=Y=Z: 1.06 Å | ||||||||||||||||||||
密度 |
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対称性 | 空間群: 1 | ||||||||||||||||||||
詳細 | EMDB XML:
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-添付データ
-ハーフマップ: #2
ファイル | emd_37268_half_map_1.map | ||||||||||||
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投影像・断面図 |
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密度ヒストグラム |
-ハーフマップ: #1
ファイル | emd_37268_half_map_2.map | ||||||||||||
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投影像・断面図 |
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密度ヒストグラム |
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試料の構成要素
-全体 : Cm-PSII-FCPII-I/J/K
全体 | 名称: Cm-PSII-FCPII-I/J/K |
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要素 |
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-超分子 #1: Cm-PSII-FCPII-I/J/K
超分子 | 名称: Cm-PSII-FCPII-I/J/K / タイプ: complex / ID: 1 / 親要素: 0 / 含まれる分子: #1-#3 詳細: Cm-PSII-FCPII-I/J/K complex in the PSII-FCPII supercomplex from Cyclotella meneghiniana |
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由来(天然) | 生物種: ![]() |
-分子 #1: FCPII-I, Fucoxanthin chlorophyll a/c binding protein
分子 | 名称: FCPII-I, Fucoxanthin chlorophyll a/c binding protein タイプ: protein_or_peptide / ID: 1 / コピー数: 1 / 光学異性体: LEVO |
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由来(天然) | 生物種: ![]() |
分子量 | 理論値: 23.27917 KDa |
配列 | 文字列: SPRALPFGSA PATLDGSLVG DVGFDPIGFS TAPFASFNNP LYKEGDFMSD LEWLREAELT HGRIAQLAVL GFIWPALFGT FPGNENFGG ADAFSYVNPL EAITHVPDLA IYQIVGGMAW VEYQRVIRIK EQGKNRVSGD IGLGYPGGWN PFNLNYSPEE Y AEKQLQEI ...文字列: SPRALPFGSA PATLDGSLVG DVGFDPIGFS TAPFASFNNP LYKEGDFMSD LEWLREAELT HGRIAQLAVL GFIWPALFGT FPGNENFGG ADAFSYVNPL EAITHVPDLA IYQIVGGMAW VEYQRVIRIK EQGKNRVSGD IGLGYPGGWN PFNLNYSPEE Y AEKQLQEI KHCRLAMIGA FGLFFQALNS GTDIVSQLSP AFAAPDYAAK AGYFLP |
-分子 #2: FCPII-K, Fucoxanthin chlorophyll a/c binding protein
分子 | 名称: FCPII-K, Fucoxanthin chlorophyll a/c binding protein タイプ: protein_or_peptide / ID: 2 / コピー数: 1 / 光学異性体: LEVO |
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由来(天然) | 生物種: ![]() |
分子量 | 理論値: 13.039064 KDa |
配列 | 文字列: (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) ...文字列: (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) |
-分子 #3: FCPII-J, Fucoxanthin chlorophyll a/c binding protein
分子 | 名称: FCPII-J, Fucoxanthin chlorophyll a/c binding protein タイプ: protein_or_peptide / ID: 3 / コピー数: 1 / 光学異性体: LEVO |
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由来(天然) | 生物種: ![]() |
分子量 | 理論値: 17.850197 KDa |
配列 | 文字列: ENEIGALAPT GFFDPAKLSD GISQEKFDQY RLAELKHGRA AMLAVLGYVA PETYRFGYDL IPGELSTRDI PNGVAALNAI PFGGWVQMI AFVGTVEAYG WFTSPTGVLD LPADILAKRQ TSELQHGRLA MLAFLELIRH DSQNLAQPGF DGYDNLITGL P FLY |
-分子 #4: CHLOROPHYLL A
分子 | 名称: CHLOROPHYLL A / タイプ: ligand / ID: 4 / コピー数: 30 / 式: CLA |
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分子量 | 理論値: 893.489 Da |
Chemical component information | ![]() ChemComp-CLA: |
-分子 #5: (3S,3'S,5R,5'R,6S,6'R,8'R)-3,5'-dihydroxy-8-oxo-6',7'-didehydro-5...
分子 | 名称: (3S,3'S,5R,5'R,6S,6'R,8'R)-3,5'-dihydroxy-8-oxo-6',7'-didehydro-5,5',6,6',7,8-hexahydro-5,6-epoxy-beta,beta-caroten-3'- yl acetate タイプ: ligand / ID: 5 / コピー数: 6 / 式: A86 |
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分子量 | 理論値: 658.906 Da |
-分子 #6: (3S,3'R,5R,6S,7cis)-7',8'-didehydro-5,6-dihydro-5,6-epoxy-beta,be...
分子 | 名称: (3S,3'R,5R,6S,7cis)-7',8'-didehydro-5,6-dihydro-5,6-epoxy-beta,beta-carotene-3,3'-diol タイプ: ligand / ID: 6 / コピー数: 2 / 式: DD6 |
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分子量 | 理論値: 582.855 Da |
Chemical component information | ![]() ChemComp-DD6: |
-実験情報
-構造解析
手法 | クライオ電子顕微鏡法 |
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![]() | 単粒子再構成法 |
試料の集合状態 | particle |
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試料調製
緩衝液 | pH: 6.5 |
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凍結 | 凍結剤: ETHANE |
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電子顕微鏡法
顕微鏡 | FEI TITAN KRIOS |
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撮影 | フィルム・検出器のモデル: GATAN K2 SUMMIT (4k x 4k) 平均電子線量: 50.0 e/Å2 |
電子線 | 加速電圧: 300 kV / 電子線源: ![]() |
電子光学系 | 照射モード: FLOOD BEAM / 撮影モード: BRIGHT FIELD / 最大 デフォーカス(公称値): 2.5 µm / 最小 デフォーカス(公称値): 1.5 µm |
実験機器 | ![]() モデル: Titan Krios / 画像提供: FEI Company |