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Open data
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Basic information
Entry | ![]() | |||||||||
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Title | Cryo-EM structure of BlCHR2 class one | |||||||||
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![]() | channelrhodopsin / MEMBRANE PROTEIN | |||||||||
Biological species | ![]() | |||||||||
Method | single particle reconstruction / cryo EM / Resolution: 2.7 Å | |||||||||
![]() | Zhang MF | |||||||||
Funding support | 1 items
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![]() | ![]() Title: Structure of BlCHR2 class one Authors: Zhang MF | |||||||||
History |
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Structure visualization
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Downloads & links
-EMDB archive
Map data | ![]() | |||
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Header (meta data) | ![]() | |||
Archive directory | ![]() ![]() | HTTPS FTP |
-Validation report
Summary document | ![]() | 1.2 MB | Display | ![]() |
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Full document | ![]() | 1.2 MB | Display | |
Data in XML | ![]() | 21.9 KB | Display | |
Data in CIF | ![]() | 25.7 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 8jxoMC ![]() 36708 ![]() 8jxpC M: atomic model generated by this map C: citing same article ( |
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Links
EMDB pages | ![]() ![]() |
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Map
File | ![]() | ||||||||||||||||||||
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Voxel size | X=Y=Z: 0.35 Å | ||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||
Details | EMDB XML:
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-Supplemental data
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Sample components
-Entire : BlCHR2
Entire | Name: BlCHR2 |
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Components |
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-Supramolecule #1: BlCHR2
Supramolecule | Name: BlCHR2 / type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1 |
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Source (natural) | Organism: ![]() |
-Macromolecule #1: BlCHR2
Macromolecule | Name: BlCHR2 / type: protein_or_peptide / ID: 1 / Details: GB:OP710242 / Number of copies: 3 / Enantiomer: LEVO |
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Source (natural) | Organism: ![]() |
Molecular weight | Theoretical: 43.984391 KDa |
Recombinant expression | Organism: ![]() |
Sequence | String: MSECCELCVC QKEPGTFGAL IAVNTITAII LVAAGAYMAW KTAAGLGWNT RPHGPEGPPE ENWLSPGISI LCGVMYAFKA IDWASYNDT GESTAFSLNQ VWYSDYLITC PLLVLDFCIT VNLRYKLVFS SSIACLLAIA VSTFIVDAPY RYYMYGIGLA G FICAGYAL ...String: MSECCELCVC QKEPGTFGAL IAVNTITAII LVAAGAYMAW KTAAGLGWNT RPHGPEGPPE ENWLSPGISI LCGVMYAFKA IDWASYNDT GESTAFSLNQ VWYSDYLITC PLLVLDFCIT VNLRYKLVFS SSIACLLAIA VSTFIVDAPY RYYMYGIGLA G FICAGYAL WNEINAQREK IPDSAWWYLS AGRLIFFAGW PFFPLLWTLS FHTSGVINEE WYFILHAILD ILCKAVFGFF ML GFRLELE ELDFKAIEAE QAKLEGDKEA QALKNDKDTG EVLRHYNRAS SGFFGQGIPD DQGSVSGGSV LMSRARRQHM LYR REASYL SMDPMAEKIR ELEMLKKKIQ KEVDSRSKKM QREMTARFAI QDDSDDEDGG RGAGSARRRK NARRG |
-Macromolecule #2: RETINAL
Macromolecule | Name: RETINAL / type: ligand / ID: 2 / Number of copies: 3 / Formula: RET |
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Molecular weight | Theoretical: 284.436 Da |
Chemical component information | ![]() ChemComp-RET: |
-Macromolecule #3: POTASSIUM ION
Macromolecule | Name: POTASSIUM ION / type: ligand / ID: 3 / Number of copies: 18 / Formula: K |
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Molecular weight | Theoretical: 39.098 Da |
-Macromolecule #4: water
Macromolecule | Name: water / type: ligand / ID: 4 / Number of copies: 9 / Formula: HOH |
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Molecular weight | Theoretical: 18.015 Da |
Chemical component information | ![]() ChemComp-HOH: |
-Experimental details
-Structure determination
Method | cryo EM |
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![]() | single particle reconstruction |
Aggregation state | particle |
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Sample preparation
Buffer | pH: 7.4 |
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Vitrification | Cryogen name: ETHANE |
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Electron microscopy
Microscope | FEI TITAN KRIOS |
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Image recording | Film or detector model: FEI FALCON IV (4k x 4k) / Average electron dose: 50.0 e/Å2 |
Electron beam | Acceleration voltage: 300 kV / Electron source: ![]() |
Electron optics | Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Nominal defocus max: 2.0 µm / Nominal defocus min: 1.2 µm |
Experimental equipment | ![]() Model: Titan Krios / Image courtesy: FEI Company |
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Image processing
Startup model | Type of model: NONE |
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Final reconstruction | Resolution.type: BY AUTHOR / Resolution: 2.7 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 47000 |
Initial angle assignment | Type: NOT APPLICABLE |
Final angle assignment | Type: NOT APPLICABLE |