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Open data
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Basic information
Entry | ![]() | |||||||||
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Title | Cryo-EM structure of ATP bound human ClC-6 | |||||||||
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![]() | MEMBRANE PROTEIN | |||||||||
Function / homology | ![]() voltage-gated chloride channel activity / cell volume homeostasis / antiporter activity / chloride transport / chloride transmembrane transporter activity / response to mechanical stimulus / monoatomic ion transmembrane transport / Stimuli-sensing channels / Signaling by BRAF and RAF1 fusions / late endosome membrane ...voltage-gated chloride channel activity / cell volume homeostasis / antiporter activity / chloride transport / chloride transmembrane transporter activity / response to mechanical stimulus / monoatomic ion transmembrane transport / Stimuli-sensing channels / Signaling by BRAF and RAF1 fusions / late endosome membrane / endosome membrane / lysosomal membrane / intracellular membrane-bounded organelle / signal transduction / ATP binding / membrane Similarity search - Function | |||||||||
Biological species | ![]() | |||||||||
Method | single particle reconstruction / cryo EM / Resolution: 3.4 Å | |||||||||
![]() | Zhang SS | |||||||||
Funding support | ![]()
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![]() | ![]() Title: Molecular basis of ClC-6 function and its impairment in human disease Authors: Zhang SS | |||||||||
History |
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Structure visualization
Supplemental images |
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Downloads & links
-EMDB archive
Map data | ![]() | 9.5 MB | ![]() | |
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Header (meta data) | ![]() ![]() | 13.9 KB 13.9 KB | Display Display | ![]() |
Images | ![]() | 197.4 KB | ||
Others | ![]() ![]() | 39.7 MB 39.7 MB | ||
Archive directory | ![]() ![]() | HTTPS FTP |
-Validation report
Summary document | ![]() | 730.4 KB | Display | ![]() |
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Full document | ![]() | 729.9 KB | Display | |
Data in XML | ![]() | 11.7 KB | Display | |
Data in CIF | ![]() | 13.4 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 8jpoMC ![]() 8jpjC ![]() 8jprC M: atomic model generated by this map C: citing same article ( |
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Similar structure data | Similarity search - Function & homology ![]() |
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Links
EMDB pages | ![]() ![]() |
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Related items in Molecule of the Month |
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Map
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Voxel size | X=Y=Z: 0.8374 Å | ||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||
Details | EMDB XML:
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-Supplemental data
-Half map: #1
File | emd_36485_half_map_1.map | ||||||||||||
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Projections & Slices |
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Density Histograms |
-Half map: #2
File | emd_36485_half_map_2.map | ||||||||||||
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Projections & Slices |
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Density Histograms |
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Sample components
-Entire : endolysosomal transporter
Entire | Name: endolysosomal transporter |
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Components |
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-Supramolecule #1: endolysosomal transporter
Supramolecule | Name: endolysosomal transporter / type: organelle_or_cellular_component / ID: 1 / Parent: 0 / Macromolecule list: #1 |
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Source (natural) | Organism: ![]() |
-Macromolecule #1: H(+)/Cl(-) exchange transporter 6
Macromolecule | Name: H(+)/Cl(-) exchange transporter 6 / type: protein_or_peptide / ID: 1 / Number of copies: 2 / Enantiomer: LEVO |
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Source (natural) | Organism: ![]() |
Molecular weight | Theoretical: 97.38343 KDa |
Recombinant expression | Organism: ![]() |
Sequence | String: MAGCRGSLCC CCRWCCCCGE RETRTPEELT ILGETQEEED EILPRKDYES LDYDRCINDP YLEVLETMDN KKGRRYEAVK WMVVFAIGV CTGLVGLFVD FFVRLFTQLK FGVVQTSVEE CSQKGCLALS LLELLGFNLT FVFLASLLVL IEPVAAGSGI P EVKCYLNG ...String: MAGCRGSLCC CCRWCCCCGE RETRTPEELT ILGETQEEED EILPRKDYES LDYDRCINDP YLEVLETMDN KKGRRYEAVK WMVVFAIGV CTGLVGLFVD FFVRLFTQLK FGVVQTSVEE CSQKGCLALS LLELLGFNLT FVFLASLLVL IEPVAAGSGI P EVKCYLNG VKVPGIVRLR TLLCKVLGVL FSVAGGLFVE KEGPMIHSGS VVGAGLPQFQ SISLRKIQFN FPYFRSDRDK RD FVSAGAA AGVAAAFGAP IGGTLFSLEE GSSFWNQGLT WKVLFCSMSA TFTLNFFRSG IQFGSWGSFQ LPGLLNFGEF KCS DSDKKC HLWTAMDLGF FVVMGVIGGL LGATFNCLNK RLAKYRMRNV HPKPKLVRVL ESLLVSLVTT VVVFVASMVL GECR QMSSS SQIGNDSFQL QVTEDVNSSI KTFFCPNDTY NDMATLFFNP QESAILQLFH QDGTFSPVTL ALFFVLYFLL ACWTY GISV PSGLFVPSLL CGAAFGRLVA NVLKSYIGLG HIYSGTFALI GAAAFLGGVV RMTISLTVIL IESTNEITYG LPIMVT LMV AKWTGDFFNK GIYDIHVGLR GVPLLEWETE VEMDKLRASD IMEPNLTYVY PHTRIQSLVS ILRTTVHHAF PVVTENR GN EKEFMKGNQL ISNNIKFKKS SILTRAGEQR KRSQSMKSYP SSELRNMCDE HIASEEPAEK EDLLQQMLER RYTPYPNL Y PDQSPSEDWT MEERFRPLTF HGLILRSQLV TLLVRGVCYS ESQSSASQPR LSYAEMAEDY PRYPDIHDLD LTLLNPRMI VDVTPYMNPS PFTVSPNTHV SQVFNLFRTM GLRHLPVVNA VGEIVGIITR HNLTYEFLQA RLRQHYQTI UniProtKB: H(+)/Cl(-) exchange transporter 6 |
-Macromolecule #2: CHLORIDE ION
Macromolecule | Name: CHLORIDE ION / type: ligand / ID: 2 / Number of copies: 4 / Formula: CL |
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Molecular weight | Theoretical: 35.453 Da |
-Macromolecule #3: MAGNESIUM ION
Macromolecule | Name: MAGNESIUM ION / type: ligand / ID: 3 / Number of copies: 2 / Formula: MG |
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Molecular weight | Theoretical: 24.305 Da |
-Macromolecule #4: ADENOSINE-5'-TRIPHOSPHATE
Macromolecule | Name: ADENOSINE-5'-TRIPHOSPHATE / type: ligand / ID: 4 / Number of copies: 2 / Formula: ATP |
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Molecular weight | Theoretical: 507.181 Da |
Chemical component information | ![]() ChemComp-ATP: |
-Experimental details
-Structure determination
Method | cryo EM |
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![]() | single particle reconstruction |
Aggregation state | particle |
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Sample preparation
Buffer | pH: 7.2 |
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Vitrification | Cryogen name: ETHANE |
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Electron microscopy
Microscope | FEI TITAN KRIOS |
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Image recording | Film or detector model: GATAN K3 BIOQUANTUM (6k x 4k) / Average electron dose: 50.0 e/Å2 |
Electron beam | Acceleration voltage: 300 kV / Electron source: ![]() |
Electron optics | Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Nominal defocus max: 2.5 µm / Nominal defocus min: 1.2 µm |
Experimental equipment | ![]() Model: Titan Krios / Image courtesy: FEI Company |
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Image processing
Startup model | Type of model: INSILICO MODEL |
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Final reconstruction | Resolution.type: BY AUTHOR / Resolution: 3.4 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 145000 |
Initial angle assignment | Type: ANGULAR RECONSTITUTION |
Final angle assignment | Type: ANGULAR RECONSTITUTION |