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- EMDB-36461: Structure of a synthetic circadian clock protein KaiC mutant of c... -
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Open data
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Basic information
Entry | ![]() | |||||||||
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Title | Structure of a synthetic circadian clock protein KaiC mutant of cyanobacteria Synechococcus elongatus PCC 7942 | |||||||||
![]() | Cryo-EM map of anKaiC | |||||||||
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![]() | circadian clock protein / Synthetic protein / KaiC / BIOSYNTHETIC PROTEIN | |||||||||
Function / homology | ![]() regulation of phosphorelay signal transduction system / negative regulation of circadian rhythm / entrainment of circadian clock / protein serine/threonine/tyrosine kinase activity / Hydrolases; Acting on acid anhydrides; Acting on acid anhydrides to facilitate cellular and subcellular movement / circadian rhythm / non-specific serine/threonine protein kinase / phosphorylation / protein serine kinase activity / protein serine/threonine kinase activity ...regulation of phosphorelay signal transduction system / negative regulation of circadian rhythm / entrainment of circadian clock / protein serine/threonine/tyrosine kinase activity / Hydrolases; Acting on acid anhydrides; Acting on acid anhydrides to facilitate cellular and subcellular movement / circadian rhythm / non-specific serine/threonine protein kinase / phosphorylation / protein serine kinase activity / protein serine/threonine kinase activity / regulation of DNA-templated transcription / magnesium ion binding / ATP hydrolysis activity / DNA binding / ATP binding / identical protein binding Similarity search - Function | |||||||||
Biological species | ![]() | |||||||||
Method | single particle reconstruction / cryo EM / Resolution: 2.51 Å | |||||||||
![]() | Jia X / Zhang Q / Li S / Guo J | |||||||||
Funding support | ![]()
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![]() | ![]() Title: Adaptation of ancient cyanobacterial clock to the day length ~ 0.95 Ga ago Authors: Li S / Wan Y | |||||||||
History |
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Structure visualization
Supplemental images |
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Downloads & links
-EMDB archive
Map data | ![]() | 96.5 MB | ![]() | |
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Header (meta data) | ![]() ![]() | 14 KB 14 KB | Display Display | ![]() |
FSC (resolution estimation) | ![]() | 10.7 KB | Display | ![]() |
Images | ![]() | 210.6 KB | ||
Filedesc metadata | ![]() | 5.5 KB | ||
Others | ![]() ![]() | 79.5 MB 79.5 MB | ||
Archive directory | ![]() ![]() | HTTPS FTP |
-Validation report
Summary document | ![]() | 1 MB | Display | ![]() |
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Full document | ![]() | 1 MB | Display | |
Data in XML | ![]() | 17.9 KB | Display | |
Data in CIF | ![]() | 23.5 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 8jonMC M: atomic model generated by this map C: citing same article ( |
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Similar structure data | Similarity search - Function & homology ![]() |
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Links
EMDB pages | ![]() ![]() |
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Related items in Molecule of the Month |
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Map
File | ![]() | ||||||||||||||||||||
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Annotation | Cryo-EM map of anKaiC | ||||||||||||||||||||
Voxel size | X=Y=Z: 0.71 Å | ||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||
Details | EMDB XML:
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-Supplemental data
-Half map: half map 1
File | emd_36461_half_map_1.map | ||||||||||||
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Annotation | half map 1 | ||||||||||||
Projections & Slices |
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Density Histograms |
-Half map: half map 2
File | emd_36461_half_map_2.map | ||||||||||||
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Annotation | half map 2 | ||||||||||||
Projections & Slices |
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Density Histograms |
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Sample components
-Entire : Synthetic KaiC protein
Entire | Name: Synthetic KaiC protein |
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Components |
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-Supramolecule #1: Synthetic KaiC protein
Supramolecule | Name: Synthetic KaiC protein / type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1 |
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Source (natural) | Organism: ![]() |
-Macromolecule #1: Circadian clock oscillator protein KaiC
Macromolecule | Name: Circadian clock oscillator protein KaiC / type: protein_or_peptide / ID: 1 / Number of copies: 6 / Enantiomer: LEVO / EC number: non-specific serine/threonine protein kinase |
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Source (natural) | Organism: ![]() |
Molecular weight | Theoretical: 58.58909 KDa |
Recombinant expression | Organism: ![]() ![]() |
Sequence | String: MTSLAEHTSP NNESSHQRLS EVQKMPTMIE GFDDISHGGL PTGRSTLVSG TSGTGKTLFS IQFLYNGITE FDEPGIFVTF EESPQDIIK NARSFGWNLQ KLVDQGKLFI LDASPDPDGQ EVAGGFDLSA LIERINYAIQ KYKARRVSID SVTAVFQQYD A ASVVRREI ...String: MTSLAEHTSP NNESSHQRLS EVQKMPTMIE GFDDISHGGL PTGRSTLVSG TSGTGKTLFS IQFLYNGITE FDEPGIFVTF EESPQDIIK NARSFGWNLQ KLVDQGKLFI LDASPDPDGQ EVAGGFDLSA LIERINYAIQ KYKARRVSID SVTAVFQQYD A ASVVRREI FRLVARLKQI GVTTVMTTER IDEYGPIARY GVEEFVSDNV VILRNVLEGE RRRRTVEILK LRGTTHMKGE YP FTINDHG INIFPLGAMR LTQRSSNVRV SSGVPRLDEM CGGGFFKDSI ILATGATGTG KTLLVSKFIE NACQNKERAI LFA YEESRA QLLRNASSWG IDFEEMERQG LLKIICAYPE SAGLEDHLQI IKSEIGDFKP SRIAIDSLSA LARGVSNNAF RQFV IGVTG YAKQEEITGF FTNTSDQFMG SHSITDSHIS TITDTILLLQ YVEIRGEMSR AINVFKMRGS WHDKGIREYV ITDKG PEIK DSFRNFERII SGSPTRITVD EKSENLSRIA RGVQEKEPES UniProtKB: Circadian clock oscillator protein KaiC |
-Macromolecule #2: ADENOSINE-5'-TRIPHOSPHATE
Macromolecule | Name: ADENOSINE-5'-TRIPHOSPHATE / type: ligand / ID: 2 / Number of copies: 6 / Formula: ATP |
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Molecular weight | Theoretical: 507.181 Da |
Chemical component information | ![]() ChemComp-ATP: |
-Macromolecule #3: MAGNESIUM ION
Macromolecule | Name: MAGNESIUM ION / type: ligand / ID: 3 / Number of copies: 12 / Formula: MG |
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Molecular weight | Theoretical: 24.305 Da |
-Macromolecule #4: ADENOSINE-5'-DIPHOSPHATE
Macromolecule | Name: ADENOSINE-5'-DIPHOSPHATE / type: ligand / ID: 4 / Number of copies: 6 / Formula: ADP |
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Molecular weight | Theoretical: 427.201 Da |
Chemical component information | ![]() ChemComp-ADP: |
-Experimental details
-Structure determination
Method | cryo EM |
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![]() | single particle reconstruction |
Aggregation state | particle |
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Sample preparation
Buffer | pH: 8 |
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Vitrification | Cryogen name: ETHANE |
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Electron microscopy
Microscope | TFS KRIOS |
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Image recording | Film or detector model: FEI FALCON IV (4k x 4k) / Average electron dose: 50.0 e/Å2 |
Electron beam | Acceleration voltage: 300 kV / Electron source: ![]() |
Electron optics | Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Nominal defocus max: 3.0 µm / Nominal defocus min: 1.0 µm |
Experimental equipment | ![]() Model: Titan Krios / Image courtesy: FEI Company |