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基本情報
登録情報 | ![]() | |||||||||||||||
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タイトル | Structure of basal beta-arrestin2 | |||||||||||||||
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![]() | GPCR / Arrestin / SIGNALING PROTEIN / SIGNALING PROTEIN-IMMUNE SYSTEM complex | |||||||||||||||
機能・相同性 | ![]() type 2A serotonin receptor binding / platelet activating factor receptor binding / postsynaptic signal transduction / negative regulation of opioid receptor signaling pathway / positive regulation of opioid receptor signaling pathway / positive regulation of synaptic transmission, dopaminergic / alpha-1A adrenergic receptor binding / follicle-stimulating hormone receptor binding / G alpha (s) signalling events / alpha-1B adrenergic receptor binding ...type 2A serotonin receptor binding / platelet activating factor receptor binding / postsynaptic signal transduction / negative regulation of opioid receptor signaling pathway / positive regulation of opioid receptor signaling pathway / positive regulation of synaptic transmission, dopaminergic / alpha-1A adrenergic receptor binding / follicle-stimulating hormone receptor binding / G alpha (s) signalling events / alpha-1B adrenergic receptor binding / follicle-stimulating hormone signaling pathway / angiotensin receptor binding / positive regulation of cardiac muscle cell differentiation / WNT5A-dependent internalization of FZD4 / protein kinase B binding / MAP2K and MAPK activation / Ub-specific processing proteases / negative regulation of natural killer cell mediated cytotoxicity / negative regulation of toll-like receptor signaling pathway / Cargo recognition for clathrin-mediated endocytosis / Clathrin-mediated endocytosis / negative regulation of interleukin-12 production / regulation of G protein-coupled receptor signaling pathway / positive regulation of calcium ion transport / arrestin family protein binding / G protein-coupled receptor internalization / type 1 angiotensin receptor binding / response to morphine / adult walking behavior / Thrombin signalling through proteinase activated receptors (PARs) / positive regulation of epithelial cell apoptotic process / mitogen-activated protein kinase binding / negative regulation of interleukin-1 beta production / positive regulation of DNA biosynthetic process / negative regulation of release of cytochrome c from mitochondria / negative regulation of smooth muscle cell apoptotic process / detection of temperature stimulus involved in sensory perception of pain / negative regulation of interleukin-6 production / positive regulation of receptor internalization / negative regulation of tumor necrosis factor production / endocytic vesicle / positive regulation of collagen biosynthetic process / D1 dopamine receptor binding / clathrin-coated pit / negative regulation of canonical NF-kappaB signal transduction / positive regulation of glial cell proliferation / negative regulation of protein ubiquitination / 14-3-3 protein binding / transforming growth factor beta receptor signaling pathway / cell chemotaxis / negative regulation of protein phosphorylation / G protein-coupled receptor binding / regulation of protein phosphorylation / modulation of chemical synaptic transmission / receptor internalization / circadian rhythm / endocytosis / positive regulation of peptidyl-tyrosine phosphorylation / protein transport / positive regulation of peptidyl-serine phosphorylation / cytoplasmic vesicle / basolateral plasma membrane / postsynaptic membrane / proteasome-mediated ubiquitin-dependent protein catabolic process / negative regulation of neuron apoptotic process / transcription by RNA polymerase II / dendritic spine / molecular adaptor activity / positive regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction / positive regulation of ERK1 and ERK2 cascade / protein ubiquitination / endosome / positive regulation of protein phosphorylation / G protein-coupled receptor signaling pathway / protein domain specific binding / signaling receptor binding / glutamatergic synapse / ubiquitin protein ligase binding / positive regulation of gene expression / protein-containing complex binding / enzyme binding / identical protein binding / nucleus / plasma membrane / cytoplasm 類似検索 - 分子機能 | |||||||||||||||
生物種 | ![]() ![]() ![]() ![]() | |||||||||||||||
手法 | 単粒子再構成法 / クライオ電子顕微鏡法 / 解像度: 3.5 Å | |||||||||||||||
![]() | Maharana J / Sarma P / Yadav MK / Chami M / Banerjee R / Shukla AK | |||||||||||||||
資金援助 | ![]()
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![]() | ![]() タイトル: Molecular insights into atypical modes of β-arrestin interaction with seven transmembrane receptors. 著者: Jagannath Maharana / Fumiya K Sano / Parishmita Sarma / Manish K Yadav / Longhan Duan / Tomasz M Stepniewski / Madhu Chaturvedi / Ashutosh Ranjan / Vinay Singh / Sayantan Saha / Gargi Mahajan ...著者: Jagannath Maharana / Fumiya K Sano / Parishmita Sarma / Manish K Yadav / Longhan Duan / Tomasz M Stepniewski / Madhu Chaturvedi / Ashutosh Ranjan / Vinay Singh / Sayantan Saha / Gargi Mahajan / Mohamed Chami / Wataru Shihoya / Jana Selent / Ka Young Chung / Ramanuj Banerjee / Osamu Nureki / Arun K Shukla / ![]() ![]() ![]() ![]() ![]() 要旨: β-arrestins (βarrs) are multifunctional proteins involved in signaling and regulation of seven transmembrane receptors (7TMRs), and their interaction is driven primarily by agonist-induced receptor ...β-arrestins (βarrs) are multifunctional proteins involved in signaling and regulation of seven transmembrane receptors (7TMRs), and their interaction is driven primarily by agonist-induced receptor activation and phosphorylation. Here, we present seven cryo-electron microscopy structures of βarrs either in the basal state, activated by the muscarinic receptor subtype 2 (M2R) through its third intracellular loop, or activated by the βarr-biased decoy D6 receptor (D6R). Combined with biochemical, cellular, and biophysical experiments, these structural snapshots allow the visualization of atypical engagement of βarrs with 7TMRs and also reveal a structural transition in the carboxyl terminus of βarr2 from a β strand to an α helix upon activation by D6R. Our study provides previously unanticipated molecular insights into the structural and functional diversity encoded in 7TMR-βarr complexes with direct implications for exploring novel therapeutic avenues. | |||||||||||||||
履歴 |
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構造の表示
添付画像 |
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ダウンロードとリンク
-EMDBアーカイブ
マップデータ | ![]() | 59.6 MB | ![]() | |
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ヘッダ (付随情報) | ![]() ![]() | 19.3 KB 19.3 KB | 表示 表示 | ![]() |
FSC (解像度算出) | ![]() | 8.4 KB | 表示 | ![]() |
画像 | ![]() | 49.3 KB | ||
Filedesc metadata | ![]() | 6.3 KB | ||
その他 | ![]() ![]() | 59.4 MB 59.4 MB | ||
アーカイブディレクトリ | ![]() ![]() | HTTPS FTP |
-検証レポート
文書・要旨 | ![]() | 724.4 KB | 表示 | ![]() |
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文書・詳細版 | ![]() | 724 KB | 表示 | |
XML形式データ | ![]() | 16 KB | 表示 | |
CIF形式データ | ![]() | 20.9 KB | 表示 | |
アーカイブディレクトリ | ![]() ![]() | HTTPS FTP |
-関連構造データ
関連構造データ | ![]() 8j9kMC ![]() 8go9C ![]() 8j8rC ![]() 8j8vC ![]() 8j8zC ![]() 8j97C ![]() 8ja3C ![]() 8jafC M: このマップから作成された原子モデル C: 同じ文献を引用 ( |
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類似構造データ | 類似検索 - 機能・相同性 ![]() |
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リンク
EMDBのページ | ![]() ![]() |
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「今月の分子」の関連する項目 |
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マップ
ファイル | ![]() | ||||||||||||||||||||
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ボクセルのサイズ | X=Y=Z: 1.2347 Å | ||||||||||||||||||||
密度 |
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対称性 | 空間群: 1 | ||||||||||||||||||||
詳細 | EMDB XML:
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-添付データ
-ハーフマップ: #2
ファイル | emd_36110_half_map_1.map | ||||||||||||
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投影像・断面図 |
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密度ヒストグラム |
-ハーフマップ: #1
ファイル | emd_36110_half_map_2.map | ||||||||||||
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投影像・断面図 |
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密度ヒストグラム |
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試料の構成要素
-全体 : beta-arrestin2 in complex with Fab6
全体 | 名称: beta-arrestin2 in complex with Fab6 |
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要素 |
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-超分子 #1: beta-arrestin2 in complex with Fab6
超分子 | 名称: beta-arrestin2 in complex with Fab6 / タイプ: complex / ID: 1 / 親要素: 0 / 含まれる分子: all |
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-超分子 #2: beta-arrestin2
超分子 | 名称: beta-arrestin2 / タイプ: complex / ID: 2 / 親要素: 1 / 含まれる分子: #1 |
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由来(天然) | 生物種: ![]() ![]() |
-超分子 #3: Fab6
超分子 | 名称: Fab6 / タイプ: complex / ID: 3 / 親要素: 1 / 含まれる分子: #2-#3 |
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由来(天然) | 生物種: ![]() ![]() |
-分子 #1: Beta-arrestin-2
分子 | 名称: Beta-arrestin-2 / タイプ: protein_or_peptide / ID: 1 / コピー数: 1 / 光学異性体: LEVO |
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由来(天然) | 生物種: ![]() ![]() |
分子量 | 理論値: 44.490906 KDa |
組換発現 | 生物種: ![]() ![]() |
配列 | 文字列: GTRVFKKSSP NCKLTVYLGK RDFVDHLDKV DPVDGVVLVD PDYLKDRKVF VTLTCAFRYG REDLDVLGLS FRKDLFIATY QAFPPMPNP PRPPTRLQDR LLKKLGQHAH PFFFTIPQNL PCSVTLQPGP EDTGKACGVD FEIRAFCAKS IEEKSHKRNS V RLIIRKVQ ...文字列: GTRVFKKSSP NCKLTVYLGK RDFVDHLDKV DPVDGVVLVD PDYLKDRKVF VTLTCAFRYG REDLDVLGLS FRKDLFIATY QAFPPMPNP PRPPTRLQDR LLKKLGQHAH PFFFTIPQNL PCSVTLQPGP EDTGKACGVD FEIRAFCAKS IEEKSHKRNS V RLIIRKVQ FAPETPGPQP SAETTRHFLM SDRRSLHLEA SLDKELYYHG EPLNVNVHVT NNSAKTVKKI RVSVRQYADI CL FSTAQYK CPVAQLEQDD QVSPSSTFCK VYTITPLLSD NREKRGLALD GQLKHEDTNL ASSTIVKEGA NKEVLGILVS YRV KVKLVV SRGGDVSVEL PFVLMHPKPH DHITLPRPQS APREIDIPVD TNLIEFDTNY ATDDDIVFED FARLRLK UniProtKB: Beta-arrestin-2 |
-分子 #2: Fab6 light chain
分子 | 名称: Fab6 light chain / タイプ: protein_or_peptide / ID: 2 / コピー数: 1 / 光学異性体: LEVO |
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由来(天然) | 生物種: ![]() ![]() |
分子量 | 理論値: 11.356607 KDa |
組換発現 | 生物種: ![]() ![]() |
配列 | 文字列: DIQMTQSPSS LSASVGDRVT ITCRASQSVS SAVAWYQQKP GKAPKLLIYS ASSLYSGVPS RFSGSRSGTD FTLTISSLQP EDFATYYCQ QSKYDGLITF GQGTKVA |
-分子 #3: Fab6 heavy chain
分子 | 名称: Fab6 heavy chain / タイプ: protein_or_peptide / ID: 3 / コピー数: 1 / 光学異性体: LEVO |
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由来(天然) | 生物種: ![]() ![]() |
分子量 | 理論値: 13.636962 KDa |
組換発現 | 生物種: ![]() ![]() |
配列 | 文字列: SEVQLVESGG GLVQPGGSLR LSCAASGFNF SSSYIHWVRQ APGKGLEWVA SISSYYGYTS YADSVKGRFT ISADTSKNTA YLQMNSLRA EDTAVYYCAR QGYYYNSYMQ GALDYWGQGT LVTVSS |
-実験情報
-構造解析
手法 | クライオ電子顕微鏡法 |
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![]() | 単粒子再構成法 |
試料の集合状態 | particle |
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試料調製
緩衝液 | pH: 7.4 |
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凍結 | 凍結剤: ETHANE |
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電子顕微鏡法
顕微鏡 | TFS GLACIOS |
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撮影 | フィルム・検出器のモデル: GATAN K3 (6k x 4k) / 平均電子線量: 55.0 e/Å2 |
電子線 | 加速電圧: 200 kV / 電子線源: ![]() |
電子光学系 | 照射モード: FLOOD BEAM / 撮影モード: BRIGHT FIELD / 最大 デフォーカス(公称値): 2.5 µm / 最小 デフォーカス(公称値): 0.5 µm |