- EMDB-35993: Cryo-EM structure of Mycobacterium tuberculosis OppABCD in the ca... -
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基本情報
登録情報
データベース: EMDB / ID: EMD-35993
タイトル
Cryo-EM structure of Mycobacterium tuberculosis OppABCD in the catalytic intermediate state
マップデータ
試料
複合体: Cryo-EM structure of Mycobacterium tuberculosis OppABCD in the catalytic intermediate state
タンパク質・ペプチド: Uncharacterized protein Rv1280c
タンパク質・ペプチド: Putative peptide transport permease protein Rv1283c
タンパク質・ペプチド: Putative peptide transport permease protein Rv1282c
タンパク質・ペプチド: Uncharacterized ABC transporter ATP-binding protein Rv1281c
リガンド: ADENOSINE-5'-TRIPHOSPHATE
リガンド: MAGNESIUM ION
リガンド: IRON/SULFUR CLUSTER
キーワード
OppABCD / ATP-Mg / Occluded state / Type I ABC importer / Membrane protein
機能・相同性
機能・相同性情報
Tolerance by Mtb to nitric oxide produced by macrophages / glutathione binding / peptide transport / protein import / peptide transmembrane transporter activity / transmembrane transporter activity / ATP-binding cassette (ABC) transporter complex / peptidoglycan-based cell wall / peptide binding / transmembrane transport ...Tolerance by Mtb to nitric oxide produced by macrophages / glutathione binding / peptide transport / protein import / peptide transmembrane transporter activity / transmembrane transporter activity / ATP-binding cassette (ABC) transporter complex / peptidoglycan-based cell wall / peptide binding / transmembrane transport / protein transport / outer membrane-bounded periplasmic space / periplasmic space / ATP hydrolysis activity / ATP binding / plasma membrane 類似検索 - 分子機能
Oligopeptide transport permease C-like, N-terminal domain / ABC transporter type 1, GsiC-like, N-terminal domain / N-terminal TM domain of oligopeptide transport permease C / Binding-prot-dependent transport system membrane comp, N-term / Oligopeptide/dipeptide ABC transporter, C-terminal / Oligopeptide/dipeptide transporter, C-terminal region / ABC transporter type 1, transmembrane domain MetI-like / MetI-like superfamily / Binding-protein-dependent transport system inner membrane component / ABC transporter integral membrane type-1 domain profile. ...Oligopeptide transport permease C-like, N-terminal domain / ABC transporter type 1, GsiC-like, N-terminal domain / N-terminal TM domain of oligopeptide transport permease C / Binding-prot-dependent transport system membrane comp, N-term / Oligopeptide/dipeptide ABC transporter, C-terminal / Oligopeptide/dipeptide transporter, C-terminal region / ABC transporter type 1, transmembrane domain MetI-like / MetI-like superfamily / Binding-protein-dependent transport system inner membrane component / ABC transporter integral membrane type-1 domain profile. / Peptide/nickel binding protein, MppA-type / Solute-binding protein family 5 domain / Solute-binding protein family 5 / Bacterial extracellular solute-binding proteins, family 5 Middle / ABC transporter-like, conserved site / ABC transporters family signature. / ABC transporter / ABC transporter-like, ATP-binding domain / ATP-binding cassette, ABC transporter-type domain profile. / ATPases associated with a variety of cellular activities / AAA+ ATPase domain / P-loop containing nucleoside triphosphate hydrolase 類似検索 - ドメイン・相同性
Putative peptide transport permease protein Rv1283c / Putative peptide transport permease protein Rv1282c / Uncharacterized protein Rv1280c / Uncharacterized ABC transporter ATP-binding protein Rv1281c 類似検索 - 構成要素
National Natural Science Foundation of China (NSFC)
32200996
中国
National Natural Science Foundation of China (NSFC)
32171217
中国
引用
ジャーナル: Nat Struct Mol Biol / 年: 2024 タイトル: An oligopeptide permease, OppABCD, requires an iron-sulfur cluster domain for functionality. 著者: Xiaolin Yang / Tianyu Hu / Jingxi Liang / Zhiqi Xiong / Zhenli Lin / Yao Zhao / Xiaoting Zhou / Yan Gao / Shan Sun / Xiuna Yang / Luke W Guddat / Haitao Yang / Zihe Rao / Bing Zhang / 要旨: Oligopeptide permease, OppABCD, belongs to the type I ABC transporter family. Its role is to import oligopeptides into bacteria for nutrient uptake and to modulate the host immune response. OppABCD ...Oligopeptide permease, OppABCD, belongs to the type I ABC transporter family. Its role is to import oligopeptides into bacteria for nutrient uptake and to modulate the host immune response. OppABCD consists of a cluster C substrate-binding protein (SBP), OppA, membrane-spanning OppB and OppC subunits, and an ATPase, OppD, that contains two nucleotide-binding domains (NBDs). Here, using cryo-electron microscopy, we determined the high-resolution structures of Mycobacterium tuberculosis OppABCD in the resting state, oligopeptide-bound pre-translocation state, AMPPNP-bound pre-catalytic intermediate state and ATP-bound catalytic intermediate state. The structures show an assembly of a cluster C SBP with its ABC translocator and a functionally required [4Fe-4S] cluster-binding domain in OppD. Moreover, the ATP-bound OppABCD structure has an outward-occluded conformation, although no substrate was observed in the transmembrane cavity. Here, we reveal an oligopeptide recognition and translocation mechanism of OppABCD, which provides a perspective on how this and other type I ABC importers facilitate bulk substrate transfer across the lipid bilayer.