- EMDB-35637: Wheat 80S ribosome stalled on AUG-Stop boron dependently with cyc... -
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基本情報
登録情報
データベース: EMDB / ID: EMD-35637
タイトル
Wheat 80S ribosome stalled on AUG-Stop boron dependently with cycloheximide
マップデータ
試料
複合体: Wheat 80S ribosome stalled on AUG-Stop boron dependently with cycloheximide
RNA: x 6種
タンパク質・ペプチド: x 74種
リガンド: x 3種
キーワード
ribosome / TRANSLATION
機能・相同性
機能・相同性情報
translation release factor complex / cytoplasmic translational termination / translation release factor activity, codon specific / sequence-specific mRNA binding / endonucleolytic cleavage to generate mature 3'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / protein-RNA complex assembly / translation regulator activity / endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / rescue of stalled ribosome / maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) ...translation release factor complex / cytoplasmic translational termination / translation release factor activity, codon specific / sequence-specific mRNA binding / endonucleolytic cleavage to generate mature 3'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / protein-RNA complex assembly / translation regulator activity / endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / rescue of stalled ribosome / maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / 90S preribosome / maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / maturation of LSU-rRNA / ribosomal large subunit biogenesis / maturation of SSU-rRNA / small-subunit processome / protein kinase C binding / rRNA processing / ribosome biogenesis / ribosome binding / ribosomal small subunit biogenesis / ribosomal small subunit assembly / small ribosomal subunit / small ribosomal subunit rRNA binding / 5S rRNA binding / large ribosomal subunit rRNA binding / cytosolic small ribosomal subunit / cytosolic large ribosomal subunit / cytoplasmic translation / rRNA binding / negative regulation of translation / ribosome / structural constituent of ribosome / ribonucleoprotein complex / translation / positive regulation of protein phosphorylation / mRNA binding / nucleolus / RNA binding / zinc ion binding / nucleus / metal ion binding / cytoplasm / cytosol 類似検索 - 分子機能
Peptide chain release factor eRF1/aRF1 / eRF1, domain 1 / eRF1 domain 2 / eRF1 domain 2 / eRF1 domain 1 / eRF1 domain 1/Pelota-like / eRF1 domain 3 / eRF1, domain 2 superfamily / eRF1 domain 3 / eRF1_1 ...Peptide chain release factor eRF1/aRF1 / eRF1, domain 1 / eRF1 domain 2 / eRF1 domain 2 / eRF1 domain 1 / eRF1 domain 1/Pelota-like / eRF1 domain 3 / eRF1, domain 2 superfamily / eRF1 domain 3 / eRF1_1 / Ribosomal protein L2, archaeal-type / Ribosomal protein L28e / Ribosomal protein L23 / Ribosomal L28e/Mak16 / Ribosomal L28e protein family / : / Ribosomal protein S26e signature. / Ribosomal protein L41 / Ribosomal protein L41 / Ribosomal protein S26e / Ribosomal protein S26e superfamily / Ribosomal protein S26e / Ribosomal protein S21e, conserved site / Ribosomal protein S21e signature. / metallochaperone-like domain / TRASH domain / Ribosomal protein S5, eukaryotic/archaeal / Ribosomal protein S19e, conserved site / Ribosomal protein S19e signature. / Small (40S) ribosomal subunit Asc1/RACK1 / Ribosomal protein S2, eukaryotic / Ribosomal protein S21e / Ribosomal protein S21e superfamily / Ribosomal protein S21e / Ribosomal protein L29e / Ribosomal L29e protein family / 40S Ribosomal protein S10 / Ribosomal protein S10, eukaryotic/archaeal / Ribosomal protein L13e, conserved site / Ribosomal protein L13e signature. / Plectin/S10, N-terminal / Plectin/S10 domain / Ribosomal protein L22e / Ribosomal protein L22e superfamily / Ribosomal L22e protein family / Ribosomal protein L38e / Ribosomal protein L38e superfamily / Ribosomal L38e protein family / Ribosomal protein S25 / S25 ribosomal protein / Ribosomal protein S2, eukaryotic/archaeal / : / Ribosomal protein S30 / Ribosomal protein S30 / 40S ribosomal protein S29/30S ribosomal protein S14 type Z / Ribosomal protein L27e, conserved site / Ribosomal protein L27e signature. / Ribosomal protein S7e signature. / Ribosomal protein L44e signature. / Ribosomal protein L10e, conserved site / Ribosomal protein L10e signature. / Ribosomal protein S3, eukaryotic/archaeal / Ribosomal protein L10e / Ribosomal protein L19, eukaryotic / Ribosomal protein L13e / Ribosomal protein L13e / Ribosomal protein S19e / Ribosomal protein S3Ae, conserved site / Ribosomal protein S19e / Ribosomal protein S3Ae signature. / Ribosomal_S19e / Ribosomal protein S27e signature. / 60S ribosomal protein L18a/ L20, eukaryotes / Ribosomal protein S4e, N-terminal, conserved site / Ribosomal protein S4e signature. / 40S ribosomal protein S4, C-terminal domain / 40S ribosomal protein S4 C-terminus / : / Ribosomal protein S19A/S15e / Ribosomal protein S8e, conserved site / Ribosomal protein L19/L19e conserved site / Ribosomal protein L19e signature. / Ribosomal protein S8e signature. / Ribosomal protein L24e, conserved site / Ribosomal protein L24e signature. / Ribosomal protein L44e / Ribosomal protein L44 / Ribosomal protein L34e, conserved site / Ribosomal protein L34e signature. / Ribosomal protein L5 eukaryotic, C-terminal / Ribosomal L18 C-terminal region / Ribosomal protein L6e signature. / Ribosomal protein S17e / Ribosomal protein S17e-like superfamily / Ribosomal S17 / 50S ribosomal protein L18Ae/60S ribosomal protein L20 and L18a / Ribosomal L40e family / Ribosomal protein L30e signature 1. / Ribosomal protein 50S-L18Ae/60S-L20/60S-L18A / Ribosomal proteins 50S-L18Ae/60S-L20/60S-L18A 類似検索 - ドメイン・相同性
Small ribosomal subunit protein uS8c / Ribosomal protein L35A / 60S ribosomal protein L29 / 40S ribosomal protein S4 / Large ribosomal subunit protein uL23 N-terminal domain-containing protein / 60S ribosomal protein L38 / Ubiquitin-like domain-containing protein / Ribosomal protein L37 / 40S ribosomal protein S6 / 60S ribosomal protein L27 ...Small ribosomal subunit protein uS8c / Ribosomal protein L35A / 60S ribosomal protein L29 / 40S ribosomal protein S4 / Large ribosomal subunit protein uL23 N-terminal domain-containing protein / 60S ribosomal protein L38 / Ubiquitin-like domain-containing protein / Ribosomal protein L37 / 40S ribosomal protein S6 / 60S ribosomal protein L27 / 60S ribosomal protein L11 / 40S ribosomal protein S17 / Large ribosomal subunit protein uL4 C-terminal domain-containing protein / Large ribosomal subunit protein eL28 / Large ribosomal subunit protein uL15/eL18 domain-containing protein / 40S ribosomal protein S8 / 60S ribosomal protein L22 / Ribosomal protein L19 / Large ribosomal subunit protein uL15/eL18 domain-containing protein / Large ribosomal subunit protein eL14 domain-containing protein / Uncharacterized protein / KOW domain-containing protein / Uncharacterized protein / 40S ribosomal protein S16 / Small ribosomal subunit protein eS1 / 40S ribosomal protein S29 / S5 DRBM domain-containing protein / 40S ribosomal protein S26 / 60S ribosomal protein L13 / 40S ribosomal protein S24 / 60S ribosomal protein L6 / 60S ribosomal protein L7a / 60S ribosomal protein L34 / 40S ribosomal protein S30 / 60S ribosomal protein L32 / 30S ribosomal protein S11, chloroplastic / 60S ribosomal protein L37a, expressed / 60S ribosomal protein L21 / Putative eukaryotic peptide chain release factor subunit 1-3 / 60S ribosomal protein L39 / Small ribosomal subunit protein eS32 / 40S ribosomal protein S7 / Ribosomal protein L17 / Ribosomal protein L13a / 60S ribosomal protein L36 / Cytoplasmatic ribosomal protein S13 / Ribosomal protein L3 / S28 ribosomal protein / 30S ribosomal protein S3, chloroplastic / Large ribosomal subunit protein uL29 / Putative ribosomal protein S18 / Ribosomal protein S20 / Ribosomal protein eL8/eL30/eS12/Gadd45 domain-containing protein / Uncharacterized protein / Plectin/eS10 N-terminal domain-containing protein / Small ribosomal subunit protein uS17 N-terminal domain-containing protein / Large ribosomal subunit protein uL18 C-terminal eukaryotes domain-containing protein / TRASH domain-containing protein / Small ribosomal subunit protein uS7c / Uncharacterized protein / 60S ribosomal protein L18a / Small ribosomal subunit protein uS2 / Large ribosomal subunit protein uL2 C-terminal domain-containing protein / 40S ribosomal protein S21 / 40S ribosomal protein S25 / Ribosomal protein L15 / 30S ribosomal protein S4, chloroplastic / Uncharacterized protein / 40S ribosomal protein S23 / 40S ribosomal protein S19 / 60S ribosomal protein L31 / Uncharacterized protein / 40S ribosomal protein S27 / Large ribosomal subunit protein uL6 alpha-beta domain-containing protein 類似検索 - 構成要素
ジャーナル: Nat Chem Biol / 年: 2024 タイトル: Boric acid intercepts 80S ribosome migration from AUG-stop by stabilizing eRF1. 著者: Mayuki Tanaka / Takeshi Yokoyama / Hironori Saito / Madoka Nishimoto / Kengo Tsuda / Naoyuki Sotta / Hideki Shigematsu / Mikako Shirouzu / Shintaro Iwasaki / Takuhiro Ito / Toru Fujiwara / 要旨: In response to environmental changes, cells flexibly and rapidly alter gene expression through translational controls. In plants, the translation of NIP5;1, a boric acid diffusion facilitator, is ...In response to environmental changes, cells flexibly and rapidly alter gene expression through translational controls. In plants, the translation of NIP5;1, a boric acid diffusion facilitator, is downregulated in response to an excess amount of boric acid in the environment through upstream open reading frames (uORFs) that consist of only AUG and stop codons. However, the molecular details of how this minimum uORF controls translation of the downstream main ORF in a boric acid-dependent manner have remained unclear. Here, by combining ribosome profiling, translation complex profile sequencing, structural analysis with cryo-electron microscopy and biochemical assays, we show that the 80S ribosome assembled at AUG-stop migrates into the subsequent RNA segment, followed by downstream translation initiation, and that boric acid impedes this process by the stable confinement of eukaryotic release factor 1 on the 80S ribosome on AUG-stop. Our results provide molecular insight into translation regulation by a minimum and environment-responsive uORF.