+
Open data
-
Basic information
Entry | ![]() | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
Title | Herg1a-herg1b closed state 2 | |||||||||
![]() | ||||||||||
![]() |
| |||||||||
![]() | channelrhodopsin / MEMBRANE PROTEIN | |||||||||
Function / homology | ![]() membrane repolarization during action potential / inward rectifier potassium channel activity / regulation of ventricular cardiac muscle cell membrane repolarization / monoatomic ion channel complex / regulation of heart rate by cardiac conduction Similarity search - Function | |||||||||
Biological species | ![]() | |||||||||
Method | single particle reconstruction / cryo EM / Resolution: 3.8 Å | |||||||||
![]() | Zhang MF | |||||||||
Funding support | 1 items
| |||||||||
![]() | ![]() Title: Structure of Herg1a-herg1b open state Authors: Zhang MF | |||||||||
History |
|
-
Structure visualization
Supplemental images |
---|
-
Downloads & links
-EMDB archive
Map data | ![]() | 229.9 MB | ![]() | |
---|---|---|---|---|
Header (meta data) | ![]() ![]() | 12.9 KB 12.9 KB | Display Display | ![]() |
Images | ![]() | 44.8 KB | ||
Filedesc metadata | ![]() | 5.5 KB | ||
Others | ![]() ![]() | 225.8 MB 225.9 MB | ||
Archive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 8io5MC ![]() 8io4C M: atomic model generated by this map C: citing same article ( |
---|---|
Similar structure data | Similarity search - Function & homology ![]() |
-
Links
EMDB pages | ![]() ![]() |
---|---|
Related items in Molecule of the Month |
-
Map
File | ![]() | ||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Voxel size | X=Y=Z: 0.849 Å | ||||||||||||||||||||
Density |
| ||||||||||||||||||||
Symmetry | Space group: 1 | ||||||||||||||||||||
Details | EMDB XML:
|
-Supplemental data
-Half map: #1
File | emd_35608_half_map_1.map | ||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Projections & Slices |
| ||||||||||||
Density Histograms |
-Half map: #2
File | emd_35608_half_map_2.map | ||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Projections & Slices |
| ||||||||||||
Density Histograms |
-
Sample components
-Entire : Herg1a-herg1b channel
Entire | Name: Herg1a-herg1b channel |
---|---|
Components |
|
-Supramolecule #1: Herg1a-herg1b channel
Supramolecule | Name: Herg1a-herg1b channel / type: complex / ID: 1 / Parent: 0 / Macromolecule list: all |
---|---|
Source (natural) | Organism: ![]() |
-Macromolecule #1: Potassium voltage-gated channel subfamily H member 2
Macromolecule | Name: Potassium voltage-gated channel subfamily H member 2 / type: protein_or_peptide / ID: 1 / Number of copies: 4 / Enantiomer: LEVO |
---|---|
Source (natural) | Organism: ![]() |
Molecular weight | Theoretical: 126.802727 KDa |
Recombinant expression | Organism: ![]() |
Sequence | String: MPVRRGHVAP QNTFLDTIIR KFEGQSRKFI IANARVENCA VIYCNDGFCE LCGYSRAEVM QRPCTCDFLH GPRTQRRAAA QIAQALLGA EERKVEIAFY RKDGSCFLCL VDVVPVKNED GAVIMFILNF EVVMEKDMVG SPAHDTNHRG PPTSWLAPGR A KTFRLKLP ...String: MPVRRGHVAP QNTFLDTIIR KFEGQSRKFI IANARVENCA VIYCNDGFCE LCGYSRAEVM QRPCTCDFLH GPRTQRRAAA QIAQALLGA EERKVEIAFY RKDGSCFLCL VDVVPVKNED GAVIMFILNF EVVMEKDMVG SPAHDTNHRG PPTSWLAPGR A KTFRLKLP ALLALTARES SVRSGGAGGA GAPGAVVVDV DLTPAAPSSE SLALDEVTAM DNHVAGLGPA EERRALVGPG SP PRSAPGQ LPSPRAHSLN PDASGSSCSL ARTRSRESCA SVRRASSADD IEAMRAGVLP PPPRHASTGA MHPLRSGLLN STS DSDLVR YRTISKIPQI TLNFVDLKGD PFLASPTSDR EIIAPKIKER THNVTEKVTQ VLSLGADVLP EYKLQAPRIH RWTI LHYSP FKAVWDWLIL LLVIYTAVFT PYSAAFLLKE TEEGPPATEC GYACQPLAVV DLIVDIMFIV DILINFRTTY VNANE EVVS HPGRIAVHYF KGWFLIDMVA AIPFDLLIFG SGSEELIGLL KTARLLRLVR VARKLDRYSE YGAAVLFLLM CTFALI AHW LACIWYAIGN MEQPHMDSRI GWLHNLGDQI GKPYNSSGLG GPSIKDKYVT ALYFTFSSLT SVGFGNVSPN TNSEKIF SI CVMLIGSLMY ASIFGNVSAI IQRLYSGTAR YHTQMLRVRE FIRFHQIPNP LRQRLEEYFQ HAWSYTNGID MNAVLKGF P ECLQADICLH LNRSLLQHCK PFRGATKGCL RALAMKFKTT HAPPGDTLVH AGDLLTALYF ISRGSIEILR GDVVVAILG KNDIFGEPLN LYARPGKSNG DVRALTYCDL HKIHRDDLLE VLDMYPEFSD HFWSSLEITF NLRDTNMIPG SPGSTELEGG FSRQRKRKL SFRRRTDKDT EQPGEVSALG PGRAGAGPSS RGRPGGPWGE SPSSGPSSPE SSEDEGPGRS SSPLRLVPFS S PRPPGEPP GGEPLMEDCE KSSDTCNPLS GAFSGVSNIF SFWGDSRGRQ YQELPRCPAP TPSLLNIPLS SPGRRPRGDV ES RLDALQR QLNRLETRLS ADMATVLQLL QRQMTLVPPA YSAVTTPGPG PTSTSPLLPV SPLPTLTLDS LSQVSQFMAC EEL PPGAPE LPQEGPTRRL SLPGQLGALT SQPLHRHGSD PGS UniProtKB: Potassium voltage-gated channel subfamily H member 2 |
-Experimental details
-Structure determination
Method | cryo EM |
---|---|
![]() | single particle reconstruction |
Aggregation state | particle |
-
Sample preparation
Buffer | pH: 7.4 |
---|---|
Vitrification | Cryogen name: ETHANE |
-
Electron microscopy
Microscope | FEI TITAN KRIOS |
---|---|
Image recording | Film or detector model: GATAN K3 (6k x 4k) / Average electron dose: 60.0 e/Å2 |
Electron beam | Acceleration voltage: 300 kV / Electron source: ![]() |
Electron optics | Illumination mode: SPOT SCAN / Imaging mode: BRIGHT FIELD / Nominal defocus max: 1.5 µm / Nominal defocus min: 1.2 µm |
Experimental equipment | ![]() Model: Titan Krios / Image courtesy: FEI Company |
-
Image processing
Startup model | Type of model: NONE |
---|---|
Final reconstruction | Resolution.type: BY AUTHOR / Resolution: 3.8 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 39000 |
Initial angle assignment | Type: NOT APPLICABLE |
Final angle assignment | Type: NOT APPLICABLE |