登録情報 データベース : EMDB / ID : EMD-35212 ダウンロードとリンクタイトル Cryo-EM structure of KCTD7 in complex with Cullin3 マップデータcryoEM map of KCTD7/CUL3 詳細 試料複合体 : complex of KCTD7 and Cullin-3複合体 : KCTD7タンパク質・ペプチド : BTB/POZ domain-containing protein KCTD7複合体 : Cullin-3 詳細 キーワード CUL3 / ubiquitination / E3 ligase / SIGNALING PROTEIN機能・相同性 機能・相同性情報分子機能 ドメイン・相同性 構成要素
liver morphogenesis / positive regulation of mitotic cell cycle phase transition / trophectodermal cellular morphogenesis / POZ domain binding / nuclear protein quality control by the ubiquitin-proteasome system / regulation protein catabolic process at postsynapse / polar microtubule / positive regulation of transporter activity / anaphase-promoting complex-dependent catabolic process / intracellular glutamate homeostasis ... liver morphogenesis / positive regulation of mitotic cell cycle phase transition / trophectodermal cellular morphogenesis / POZ domain binding / nuclear protein quality control by the ubiquitin-proteasome system / regulation protein catabolic process at postsynapse / polar microtubule / positive regulation of transporter activity / anaphase-promoting complex-dependent catabolic process / intracellular glutamate homeostasis / COPII vesicle coating / stem cell division / Neddylation / Antigen processing: Ubiquitination & Proteasome degradation / RHOBTB3 ATPase cycle / embryonic cleavage / intracellular potassium ion homeostasis / cell projection organization / positive regulation of mitotic metaphase/anaphase transition / membrane hyperpolarization / Notch binding / RHOBTB1 GTPase cycle / fibroblast apoptotic process / negative regulation of Rho protein signal transduction / negative regulation of type I interferon production / ubiquitin ligase complex scaffold activity / mitotic metaphase chromosome alignment / Cul3-RING ubiquitin ligase complex / stress fiber assembly / positive regulation of cytokinesis / protein monoubiquitination / sperm flagellum / protein autoubiquitination / RHOBTB2 GTPase cycle / protein K48-linked ubiquitination / endoplasmic reticulum to Golgi vesicle-mediated transport / gastrulation / positive regulation of TORC1 signaling / intrinsic apoptotic signaling pathway / cyclin binding / positive regulation of protein ubiquitination / integrin-mediated signaling pathway / Degradation of DVL / cellular response to amino acid stimulus / Hedgehog 'on' state / protein destabilization / protein homooligomerization / mitotic spindle / Wnt signaling pathway / spindle pole / G1/S transition of mitotic cell cycle / Regulation of RAS by GAPs / protein polyubiquitination / KEAP1-NFE2L2 pathway / ubiquitin protein ligase activity / Antigen processing: Ubiquitination & Proteasome degradation / cell migration / Neddylation / gene expression / ubiquitin-dependent protein catabolic process / postsynapse / proteasome-mediated ubiquitin-dependent protein catabolic process / Potential therapeutics for SARS / protein ubiquitination / inflammatory response / centrosome / glutamatergic synapse / ubiquitin protein ligase binding / positive regulation of cell population proliferation / Golgi apparatus / negative regulation of transcription by RNA polymerase II / extracellular exosome / nucleoplasm / identical protein binding / membrane / nucleus / plasma membrane / cytoplasm / cytosol 類似検索 - 分子機能 Potassium channel tetramerisation-type BTB domain / BTB/POZ domain / Cullin protein neddylation domain / Cullin, conserved site / Cullin family signature. / Cullin / Cullin repeat-like-containing domain superfamily / Cullin protein, neddylation domain / Cullin protein neddylation domain / Cullin ... Potassium channel tetramerisation-type BTB domain / BTB/POZ domain / Cullin protein neddylation domain / Cullin, conserved site / Cullin family signature. / Cullin / Cullin repeat-like-containing domain superfamily / Cullin protein, neddylation domain / Cullin protein neddylation domain / Cullin / Cullin, N-terminal / Cullin homology domain / Cullin homology domain superfamily / Cullin family / Cullin family profile. / Broad-Complex, Tramtrack and Bric a brac / BTB/POZ domain / SKP1/BTB/POZ domain superfamily / Winged helix DNA-binding domain superfamily / Winged helix-like DNA-binding domain superfamily 類似検索 - ドメイン・相同性 Cullin-3 / BTB/POZ domain-containing protein KCTD7 類似検索 - 構成要素生物種 Mus musculus (ハツカネズミ) / Homo sapiens (ヒト)手法 単粒子再構成法 / クライオ電子顕微鏡法 / 解像度 : 2.8 Å 詳細 データ登録者Jiang W / Wang W / Zheng S 資金援助 中国, 1件 詳細 詳細を隠すOrganization Grant number 国 Ministry of Science and Technology (MoST, China) 中国
引用ジャーナル : Sci Adv / 年 : 2023タイトル : Structural basis for the ubiquitination of G protein βγ subunits by KCTD5/Cullin3 E3 ligase.著者 : Wentong Jiang / Wei Wang / Yinfei Kong / Sanduo Zheng / 要旨 : G protein-coupled receptor (GPCR) signaling is precisely controlled to avoid overstimulation that results in detrimental consequences. Gβγ signaling is negatively regulated by a Cullin3 (Cul3)- ... G protein-coupled receptor (GPCR) signaling is precisely controlled to avoid overstimulation that results in detrimental consequences. Gβγ signaling is negatively regulated by a Cullin3 (Cul3)-dependent E3 ligase, KCTD5, which triggers ubiquitination and degradation of free Gβγ. Here, we report the cryo-electron microscopy structures of the KCTD5-Gβγ fusion complex and the KCTD7-Cul3 complex. KCTD5 in pentameric form engages symmetrically with five copies of Gβγ through its C-terminal domain. The unique pentameric assembly of the KCTD5/Cul3 E3 ligase places the ubiquitin-conjugating enzyme (E2) and the modification sites of Gβγ in close proximity and allows simultaneous transfer of ubiquitin from E2 to five Gβγ subunits. Moreover, we show that ubiquitination of Gβγ by KCTD5 is important for fine-tuning cyclic adenosine 3´,5´-monophosphate signaling of GPCRs. Our studies provide unprecedented insights into mechanisms of substrate recognition by unusual pentameric E3 ligases and highlight the KCTD family as emerging regulators of GPCR signaling. 履歴 登録 2023年1月31日 - ヘッダ(付随情報) 公開 2023年7月26日 - マップ公開 2023年7月26日 - 更新 2024年7月3日 - 現状 2024年7月3日 処理サイト : PDBj / 状態 : 公開
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