+Open data
-Basic information
Entry | Database: EMDB / ID: EMD-34519 | |||||||||
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Title | Hsp90-AhR-p23-XAP2 complex | |||||||||
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Keywords | Hsp90 / AhR / PASB doamin / complex / p23 / XAP2 / CYTOSOLIC PROTEIN | |||||||||
Function / homology | Function and homology information circumferential growth involved in left ventricle morphogenesis / negative regulation of calcium ion transmembrane transport / cellular response to 3-methylcholanthrene / regulation of heart growth / GAF domain binding / ESR-mediated signaling / cytosolic aryl hydrocarbon receptor complex / glomerulus morphogenesis / DDX58/IFIH1-mediated induction of interferon-alpha/beta / The NLRP3 inflammasome ...circumferential growth involved in left ventricle morphogenesis / negative regulation of calcium ion transmembrane transport / cellular response to 3-methylcholanthrene / regulation of heart growth / GAF domain binding / ESR-mediated signaling / cytosolic aryl hydrocarbon receptor complex / glomerulus morphogenesis / DDX58/IFIH1-mediated induction of interferon-alpha/beta / The NLRP3 inflammasome / gland development / HSF1-dependent transactivation / reactive oxygen species biosynthetic process / Phase I - Functionalization of compounds / Xenobiotics / Aryl hydrocarbon receptor signalling / HSF1 activation / kidney morphogenesis / RHOBTB2 GTPase cycle / lung saccule development / ooplasm / Sema3A PAK dependent Axon repulsion / omega-hydroxylase P450 pathway / prostaglandin-E synthase / prostaglandin-E synthase activity / arachidonate omega-hydroxylase activity / positive regulation of growth rate / Attenuation phase / lymphocyte homeostasis / regulation of adaptive immune response / receptor ligand inhibitor activity / HSP90 chaperone cycle for steroid hormone receptors (SHR) in the presence of ligand / negative regulation of complement-dependent cytotoxicity / cellular response to 2,3,7,8-tetrachlorodibenzodioxine / telomerase activity / Endogenous sterols / nuclear aryl hydrocarbon receptor complex / HSP90-CDC37 chaperone complex / Synthesis of Prostaglandins (PG) and Thromboxanes (TX) / negative regulation of cyclic-nucleotide phosphodiesterase activity / nuclear receptor-mediated glucocorticoid signaling pathway / sperm head plasma membrane / cardiac left ventricle morphogenesis / The role of GTSE1 in G2/M progression after G2 checkpoint / negative regulation of proteasomal protein catabolic process / negative regulation of osteoblast proliferation / prostate gland development / reproductive structure development / cellular response to toxic substance / aryl hydrocarbon receptor complex / negative regulation of T cell mediated immune response to tumor cell / Regulation of actin dynamics for phagocytic cup formation / dynein axonemal particle / histone methyltransferase binding / B-1 B cell homeostasis / Estrogen-dependent gene expression / regulation of protein kinase A signaling / COP9 signalosome / post-embryonic hemopoiesis / E-box binding / negative regulation of DNA biosynthetic process / protein targeting to mitochondrion / positive regulation of protein localization to cell surface / ATP-dependent protein binding / vasculature development / camera-type eye development / negative regulation of systemic arterial blood pressure / telomerase holoenzyme complex / blood circulation / glycogen biosynthetic process / protein folding chaperone complex / negative regulation of protein metabolic process / blood vessel morphogenesis / prostaglandin biosynthetic process / protein maturation by protein folding / dATP binding / sulfonylurea receptor binding / CTP binding / UTP binding / skin development / negative regulation of vasoconstriction / telomerase holoenzyme complex assembly / branching involved in blood vessel morphogenesis / immune system process / prostaglandin metabolic process / negative regulation of osteoblast differentiation / heterocyclic compound binding / TPR domain binding / blood vessel development / positive regulation of transforming growth factor beta receptor signaling pathway / T cell homeostasis / dendritic growth cone / positive regulation of phosphorylation / aryl hydrocarbon receptor binding / B cell homeostasis / : / regulation of protein ubiquitination / chaperone cofactor-dependent protein refolding / telomere maintenance via telomerase / positive regulation of cell size Similarity search - Function | |||||||||
Biological species | Mus musculus (house mouse) | |||||||||
Method | single particle reconstruction / cryo EM / Resolution: 3.2 Å | |||||||||
Authors | Wen ZL / Zhai YJ / Zhu Y / Sun F | |||||||||
Funding support | 1 items
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Citation | Journal: Structure / Year: 2023 Title: Cryo-EM structure of the cytosolic AhR complex. Authors: Zuoling Wen / Yuebin Zhang / Beirong Zhang / Yumo Hang / Li Xu / Yangsheng Chen / Qunhui Xie / Qun Zhao / Lihua Zhang / Guohui Li / Bin Zhao / Fei Sun / Yujia Zhai / Yun Zhu / Abstract: Aryl hydrocarbon receptor (AhR) is an important ligand-activated transcription factor involved in the regulation of various important physiological functions. Here, we report the cryo-EM structures ...Aryl hydrocarbon receptor (AhR) is an important ligand-activated transcription factor involved in the regulation of various important physiological functions. Here, we report the cryo-EM structures of the Hsp90-AhR-p23 complex with or without bound XAP2, where the structure of the mouse AhR PAS-B domain is resolved. A highly conserved bridge motif of AhR is responsible for the interaction with the Hsp90 dimeric lumen. The ligand-free AhR PAS-B domain is attached to the Hsp90 dimer and is stabilized in the complex with bound XAP2. In addition, the DE-loop and a group of conserved pocket inner residues in the AhR PAS-B domain are found to be important for ligand binding. These results reveal the structural basis of the biological functions of AhR. Moreover, the protein purification method presented here allows the isolation of stable mouse AhR protein, which could be used to develop high-sensitivity biosensors for environmental pollutant detection. | |||||||||
History |
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-Structure visualization
Supplemental images |
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-Downloads & links
-EMDB archive
Map data | emd_34519.map.gz | 6.5 MB | EMDB map data format | |
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Header (meta data) | emd-34519-v30.xml emd-34519.xml | 20.6 KB 20.6 KB | Display Display | EMDB header |
FSC (resolution estimation) | emd_34519_fsc.xml | 9.1 KB | Display | FSC data file |
Images | emd_34519.png | 124.6 KB | ||
Filedesc metadata | emd-34519.cif.gz | 6.6 KB | ||
Others | emd_34519_half_map_1.map.gz emd_34519_half_map_2.map.gz | 49.6 MB 49.6 MB | ||
Archive directory | http://ftp.pdbj.org/pub/emdb/structures/EMD-34519 ftp://ftp.pdbj.org/pub/emdb/structures/EMD-34519 | HTTPS FTP |
-Related structure data
Related structure data | 8h77MC 7y04C M: atomic model generated by this map C: citing same article (ref.) |
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Similar structure data | Similarity search - Function & homologyF&H Search |
-Links
EMDB pages | EMDB (EBI/PDBe) / EMDataResource |
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Related items in Molecule of the Month |
-Map
File | Download / File: emd_34519.map.gz / Format: CCP4 / Size: 64 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) | ||||||||||||||||||||
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Voxel size | X=Y=Z: 1.07 Å | ||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||
Details | EMDB XML:
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-Supplemental data
-Half map: #2
File | emd_34519_half_map_1.map | ||||||||||||
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Projections & Slices |
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Density Histograms |
-Half map: #1
File | emd_34519_half_map_2.map | ||||||||||||
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Projections & Slices |
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Density Histograms |
-Sample components
+Entire : mouse Hsp90-AhR-p23-XAP2 complex
+Supramolecule #1: mouse Hsp90-AhR-p23-XAP2 complex
+Supramolecule #2: Heat shock protein HSP 90-beta
+Supramolecule #3: Prostaglandin E synthase 3
+Supramolecule #4: Aryl hydrocarbon receptor
+Supramolecule #5: AH receptor-interacting protein
+Macromolecule #1: Heat shock protein HSP 90-beta
+Macromolecule #2: Prostaglandin E synthase 3
+Macromolecule #3: Aryl hydrocarbon receptor
+Macromolecule #4: AH receptor-interacting protein
+Macromolecule #5: ADENOSINE-5'-DIPHOSPHATE
+Macromolecule #6: BERYLLIUM TRIFLUORIDE ION
-Experimental details
-Structure determination
Method | cryo EM |
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Processing | single particle reconstruction |
Aggregation state | particle |
-Sample preparation
Buffer | pH: 7.5 |
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Vitrification | Cryogen name: ETHANE |
-Electron microscopy
Microscope | FEI TITAN KRIOS |
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Image recording | Film or detector model: GATAN K3 (6k x 4k) / Average electron dose: 60.0 e/Å2 |
Electron beam | Acceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN |
Electron optics | Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Nominal defocus max: 1.8 µm / Nominal defocus min: 1.2 µm |
Experimental equipment | Model: Titan Krios / Image courtesy: FEI Company |