+Open data
-Basic information
Entry | Database: EMDB / ID: EMD-33982 | |||||||||
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Title | Cryo-EM structure of RCK1 mutated human Slo1 apo | |||||||||
Map data | ||||||||||
Sample |
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Keywords | TRANSPORT PROTEIN | |||||||||
Function / homology | Function and homology information monoatomic ion-gated channel activity / ligand-gated monoatomic cation channel activity / potassium channel activity / membrane Similarity search - Function | |||||||||
Biological species | Homo sapiens (human) | |||||||||
Method | single particle reconstruction / cryo EM / Resolution: 3.9 Å | |||||||||
Authors | Yamanouchi D / Nureki O | |||||||||
Funding support | Japan, 1 items
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Citation | Journal: To Be Published Title: Structural basis for dual allosteric gating modulation of Slo1-LRRC channel complex Authors: Yamanouchi D | |||||||||
History |
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-Structure visualization
Supplemental images |
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-Downloads & links
-EMDB archive
Map data | emd_33982.map.gz | 96.2 MB | EMDB map data format | |
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Header (meta data) | emd-33982-v30.xml emd-33982.xml | 13.6 KB 13.6 KB | Display Display | EMDB header |
FSC (resolution estimation) | emd_33982_fsc.xml | 10.7 KB | Display | FSC data file |
Images | emd_33982.png | 60.9 KB | ||
Masks | emd_33982_msk_1.map | 103 MB | Mask map | |
Filedesc metadata | emd-33982.cif.gz | 5.8 KB | ||
Others | emd_33982_half_map_1.map.gz emd_33982_half_map_2.map.gz | 80.8 MB 80.9 MB | ||
Archive directory | http://ftp.pdbj.org/pub/emdb/structures/EMD-33982 ftp://ftp.pdbj.org/pub/emdb/structures/EMD-33982 | HTTPS FTP |
-Validation report
Summary document | emd_33982_validation.pdf.gz | 1.1 MB | Display | EMDB validaton report |
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Full document | emd_33982_full_validation.pdf.gz | 1.1 MB | Display | |
Data in XML | emd_33982_validation.xml.gz | 17.5 KB | Display | |
Data in CIF | emd_33982_validation.cif.gz | 22.7 KB | Display | |
Arichive directory | https://ftp.pdbj.org/pub/emdb/validation_reports/EMD-33982 ftp://ftp.pdbj.org/pub/emdb/validation_reports/EMD-33982 | HTTPS FTP |
-Related structure data
Related structure data | 7yo5MC 7ynzC 7yo0C 7yo1C 7yo2C 7yo3C 7yo4C M: atomic model generated by this map C: citing same article (ref.) |
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Similar structure data | Similarity search - Function & homologyF&H Search |
-Links
EMDB pages | EMDB (EBI/PDBe) / EMDataResource |
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-Map
File | Download / File: emd_33982.map.gz / Format: CCP4 / Size: 103 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) | ||||||||||||||||||||||||||||||||||||
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Projections & slices | Image control
Images are generated by Spider. | ||||||||||||||||||||||||||||||||||||
Voxel size | X=Y=Z: 0.996 Å | ||||||||||||||||||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||||||||||||||||||
Details | EMDB XML:
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-Supplemental data
-Mask #1
File | emd_33982_msk_1.map | ||||||||||||
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Projections & Slices |
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Density Histograms |
-Half map: #1
File | emd_33982_half_map_1.map | ||||||||||||
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Projections & Slices |
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Density Histograms |
-Half map: #2
File | emd_33982_half_map_2.map | ||||||||||||
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Projections & Slices |
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Density Histograms |
-Sample components
-Entire : human Slo1-LRRC26 complex
Entire | Name: human Slo1-LRRC26 complex |
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Components |
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-Supramolecule #1: human Slo1-LRRC26 complex
Supramolecule | Name: human Slo1-LRRC26 complex / type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1 |
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Source (natural) | Organism: Homo sapiens (human) |
Molecular weight | Theoretical: 616 kDa/nm |
-Macromolecule #1: Calcium-activated potassium channel subunit alpha-1
Macromolecule | Name: Calcium-activated potassium channel subunit alpha-1 / type: protein_or_peptide / ID: 1 / Number of copies: 4 / Enantiomer: LEVO |
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Source (natural) | Organism: Homo sapiens (human) |
Molecular weight | Theoretical: 119.370484 KDa |
Recombinant expression | Organism: Homo sapiens (human) |
Sequence | String: MDALIIPVTM EVPCDSRGQR MWWAFLASSM VTFFGGLFII LLWRTLKYLW TVCCHCGGKT KEAQKINNGS SQADGTLKPV DEKEEAVAA EVGWMTSVKD WAGVMISAQT LTGRVLVVLV FALSIGALVI YFIDSSNPIE SCQNFYKDFT LQIDMAFNVF F LLYFGLRF ...String: MDALIIPVTM EVPCDSRGQR MWWAFLASSM VTFFGGLFII LLWRTLKYLW TVCCHCGGKT KEAQKINNGS SQADGTLKPV DEKEEAVAA EVGWMTSVKD WAGVMISAQT LTGRVLVVLV FALSIGALVI YFIDSSNPIE SCQNFYKDFT LQIDMAFNVF F LLYFGLRF IAANDKLWFW LEVNSVVDFF TVPPVFVSVY LNRSWLGLRF LRALRLIQFS EILQFLNILK TSNSIKLVNL LS IFISTWL TAAGFIHLVE NSGDPWENFQ NNQALTYWEC VYLLMVTMST VGYGDVYAKT TLGRLFMVFF ILGGLAMFAS YVP EIIELI GNRKKYGGSY SAVSGRKHIV VCGHITLESV SNFLKAFLHK ARDDVNVEIV FLHNISPNLE LEALFKRHFT QVEF YQGSV LNPHDLARVK IESADACLIL ANKYCADPDA EDASNIMRVI SIKNYHPKIR IITQMLQYHN KAHLLNIPSW NWKEG DDAI CLAELKLGFI AQSCLAQGLS TMLANLFSMR SFIKIEEDTW QKYYLEGVSN EMYTEYLSSA FVGLSFPTVC ELCFVK LKL LMIAIEYKSA NRESRSRSRI LINPGNHLKI QEGTLGFFIA SDAKEVKRAF FYCKACHDDI TDPKRIKKCG CKRLEDE QP STLSPKKKQR NGGMRNSPNT SPKLMRHDPL LIPGNDQIDN MDSNVKKYDS TGMFHWCAPK EIEKVILTRS EAAMTVLS G HVVVCIFGDV SSALIGLRNL VMPLRASNFH YHELKHIVFV GSIEYLKREW ETLHNFPKVS ILPGTPLSRA DLRAVNINL CDMCVILSAN QNNIDDTSLQ DKECILASLN IKSMQFDDSI GVLQANSQGF TPPGMDRSSP DNSPVHGMLR QPSITTGVNI PIITELVND TNVQFLDQDD DDDPDTELYL TQPFACGTAF AVSVLDSLMS ATYFNDNILT LIRTLVTGGA TPELEALIAE E NALRGGYS TPQTLANRDR CRVAQLALLD GPFADLGDGG CYGDLFCKAL KTYNMLCFGI YRLRDAHLST PSQCTKRYVI TN PPYEFEL VPTDLIFCLM QFD UniProtKB: Calcium-activated potassium channel subunit alpha-1 |
-Macromolecule #2: CALCIUM ION
Macromolecule | Name: CALCIUM ION / type: ligand / ID: 2 / Number of copies: 8 / Formula: CA |
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Molecular weight | Theoretical: 40.078 Da |
-Experimental details
-Structure determination
Method | cryo EM |
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Processing | single particle reconstruction |
Aggregation state | particle |
-Sample preparation
Concentration | 2.0 mg/mL |
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Buffer | pH: 8 |
Vitrification | Cryogen name: ETHANE |
-Electron microscopy
Microscope | FEI TITAN KRIOS |
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Image recording | Film or detector model: GATAN K3 (6k x 4k) / Average electron dose: 48.0 e/Å2 |
Electron beam | Acceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN |
Electron optics | Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Nominal defocus max: 1.6 µm / Nominal defocus min: 0.8 µm |
Experimental equipment | Model: Titan Krios / Image courtesy: FEI Company |