+データを開く
-基本情報
登録情報 | データベース: EMDB / ID: EMD-32699 | |||||||||
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タイトル | Galanin-bound galanin receptor 2 in complex with Gq | |||||||||
マップデータ | ||||||||||
試料 |
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機能・相同性 | 機能・相同性情報 galanin-activated signaling pathway / galanin receptor binding / type 1 galanin receptor binding / type 2 galanin receptor binding / type 3 galanin receptor binding / galanin receptor activity / positive regulation of large conductance calcium-activated potassium channel activity / positive regulation of timing of catagen / positive regulation of cortisol secretion / regulation of glucocorticoid metabolic process ...galanin-activated signaling pathway / galanin receptor binding / type 1 galanin receptor binding / type 2 galanin receptor binding / type 3 galanin receptor binding / galanin receptor activity / positive regulation of large conductance calcium-activated potassium channel activity / positive regulation of timing of catagen / positive regulation of cortisol secretion / regulation of glucocorticoid metabolic process / inositol phosphate metabolic process / negative regulation of lymphocyte proliferation / phosphatidylinositol metabolic process / neuropeptide hormone activity / G-protein activation / Activation of the phototransduction cascade / Glucagon-type ligand receptors / Thromboxane signalling through TP receptor / Sensory perception of sweet, bitter, and umami (glutamate) taste / G beta:gamma signalling through PI3Kgamma / G beta:gamma signalling through CDC42 / Cooperation of PDCL (PhLP1) and TRiC/CCT in G-protein beta folding / neuropeptide binding / Activation of G protein gated Potassium channels / Inhibition of voltage gated Ca2+ channels via Gbeta/gamma subunits / Ca2+ pathway / G alpha (z) signalling events / Vasopressin regulates renal water homeostasis via Aquaporins / Glucagon-like Peptide-1 (GLP1) regulates insulin secretion / feeding behavior / Adrenaline,noradrenaline inhibits insulin secretion / ADP signalling through P2Y purinoceptor 12 / G alpha (q) signalling events / G alpha (i) signalling events / Thrombin signalling through proteinase activated receptors (PARs) / Activation of G protein gated Potassium channels / G-protein activation / G beta:gamma signalling through PI3Kgamma / Prostacyclin signalling through prostacyclin receptor / G beta:gamma signalling through PLC beta / ADP signalling through P2Y purinoceptor 1 / Thromboxane signalling through TP receptor / Presynaptic function of Kainate receptors / G beta:gamma signalling through CDC42 / Inhibition of voltage gated Ca2+ channels via Gbeta/gamma subunits / Glucagon-type ligand receptors / Adrenaline,noradrenaline inhibits insulin secretion / G alpha (12/13) signalling events / G beta:gamma signalling through BTK / alkylglycerophosphoethanolamine phosphodiesterase activity / ADP signalling through P2Y purinoceptor 12 / insulin secretion / Cooperation of PDCL (PhLP1) and TRiC/CCT in G-protein beta folding / Thrombin signalling through proteinase activated receptors (PARs) / Ca2+ pathway / G alpha (z) signalling events / Extra-nuclear estrogen signaling / G alpha (s) signalling events / G alpha (q) signalling events / photoreceptor outer segment membrane / G alpha (i) signalling events / Glucagon-like Peptide-1 (GLP1) regulates insulin secretion / spectrin binding / Vasopressin regulates renal water homeostasis via Aquaporins / peptide hormone binding / response to immobilization stress / photoreceptor outer segment / neuropeptide signaling pathway / cardiac muscle cell apoptotic process / protein kinase A signaling / photoreceptor inner segment / Peptide ligand-binding receptors / cAMP-mediated signaling / secretory granule / muscle contraction / response to insulin / adenylate cyclase-modulating G protein-coupled receptor signaling pathway / adenylate cyclase-activating G protein-coupled receptor signaling pathway / cilium / cellular response to catecholamine stimulus / response to estrogen / sensory perception of taste / adenylate cyclase-activating dopamine receptor signaling pathway / cellular response to prostaglandin E stimulus / G-protein beta-subunit binding / heterotrimeric G-protein complex / neuron projection development / signaling receptor complex adaptor activity / GTPase binding / retina development in camera-type eye / phospholipase C-activating G protein-coupled receptor signaling pathway / positive regulation of cytosolic calcium ion concentration / cell body / G alpha (i) signalling events / cellular response to hypoxia / cell population proliferation / learning or memory / cell surface receptor signaling pathway / response to xenobiotic stimulus / positive regulation of apoptotic process 類似検索 - 分子機能 | |||||||||
生物種 | Homo sapiens (ヒト) / Rattus (ネズミ) / Lama glama (ラマ) / Bos taurus (ウシ) | |||||||||
手法 | 単粒子再構成法 / クライオ電子顕微鏡法 / 解像度: 2.6 Å | |||||||||
データ登録者 | Duan J / Shen DD / Xu HE / Zhang Y / Jiang Y | |||||||||
資金援助 | 中国, 1件
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引用 | ジャーナル: Nat Commun / 年: 2022 タイトル: Molecular basis for allosteric agonism and G protein subtype selectivity of galanin receptors 著者: Duan J / Shen DD / Zhao T / Guo S / He X / Yin W / Xu P / Ji Y / Chen LN / Liu J / Zhang H / Liu Q / Shi Y / Cheng X / Jiang H / Eric Xu H / Zhang Y / Xie X / Jiang Y | |||||||||
履歴 |
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-構造の表示
添付画像 |
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-ダウンロードとリンク
-EMDBアーカイブ
マップデータ | emd_32699.map.gz | 40 MB | EMDBマップデータ形式 | |
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ヘッダ (付随情報) | emd-32699-v30.xml emd-32699.xml | 19.4 KB 19.4 KB | 表示 表示 | EMDBヘッダ |
画像 | emd_32699.png | 51.5 KB | ||
アーカイブディレクトリ | http://ftp.pdbj.org/pub/emdb/structures/EMD-32699 ftp://ftp.pdbj.org/pub/emdb/structures/EMD-32699 | HTTPS FTP |
-検証レポート
文書・要旨 | emd_32699_validation.pdf.gz | 491.6 KB | 表示 | EMDB検証レポート |
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文書・詳細版 | emd_32699_full_validation.pdf.gz | 491.2 KB | 表示 | |
XML形式データ | emd_32699_validation.xml.gz | 5.9 KB | 表示 | |
CIF形式データ | emd_32699_validation.cif.gz | 6.7 KB | 表示 | |
アーカイブディレクトリ | https://ftp.pdbj.org/pub/emdb/validation_reports/EMD-32699 ftp://ftp.pdbj.org/pub/emdb/validation_reports/EMD-32699 | HTTPS FTP |
-関連構造データ
関連構造データ | 7wq4MC 7wq3C M: このマップから作成された原子モデル C: 同じ文献を引用 (文献) |
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類似構造データ | 類似検索 - 機能・相同性F&H 検索 |
-リンク
EMDBのページ | EMDB (EBI/PDBe) / EMDataResource |
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「今月の分子」の関連する項目 |
-マップ
ファイル | ダウンロード / ファイル: emd_32699.map.gz / 形式: CCP4 / 大きさ: 42.9 MB / タイプ: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) | ||||||||||||||||||||
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ボクセルのサイズ | X=Y=Z: 1.014 Å | ||||||||||||||||||||
密度 |
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対称性 | 空間群: 1 | ||||||||||||||||||||
詳細 | EMDB XML:
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-添付データ
-試料の構成要素
+全体 : Galanin-GAL2R-Gq-Nb35
+超分子 #1: Galanin-GAL2R-Gq-Nb35
+超分子 #2: Galanin receptor type 2
+超分子 #3: Galanin
+超分子 #4: Engineered Guanine nucleotide-binding protein G(q) subunit alpha
+超分子 #5: Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1
+超分子 #6: Nb35
+超分子 #7: Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2
+分子 #1: Galanin receptor type 2
+分子 #2: Galanin
+分子 #3: Engineered Guanine nucleotide-binding protein G(q) subunit alpha
+分子 #4: Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1
+分子 #5: Nb35
+分子 #6: Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2
+分子 #7: CHOLESTEROL
-実験情報
-構造解析
手法 | クライオ電子顕微鏡法 |
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解析 | 単粒子再構成法 |
試料の集合状態 | particle |
-試料調製
緩衝液 | pH: 7.4 |
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凍結 | 凍結剤: ETHANE |
-電子顕微鏡法
顕微鏡 | FEI TITAN KRIOS |
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撮影 | フィルム・検出器のモデル: GATAN K2 SUMMIT (4k x 4k) 平均電子線量: 64.0 e/Å2 |
電子線 | 加速電圧: 300 kV / 電子線源: FIELD EMISSION GUN |
電子光学系 | 照射モード: FLOOD BEAM / 撮影モード: BRIGHT FIELD / 最大 デフォーカス(公称値): 2.5 µm / 最小 デフォーカス(公称値): 0.5 µm |
実験機器 | モデル: Titan Krios / 画像提供: FEI Company |
-画像解析
初期モデル | モデルのタイプ: PDB ENTRY PDBモデル - PDB ID: |
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最終 再構成 | 解像度のタイプ: BY AUTHOR / 解像度: 2.6 Å / 解像度の算出法: FSC 0.143 CUT-OFF / 使用した粒子像数: 255766 |
初期 角度割当 | タイプ: MAXIMUM LIKELIHOOD |
最終 角度割当 | タイプ: MAXIMUM LIKELIHOOD |