- EMDB-31890: Cryo-EM structure of Chlamydomonas TOC-TIC supercomplex -
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Basic information
Entry
Database: EMDB / ID: EMD-31890
Title
Cryo-EM structure of Chlamydomonas TOC-TIC supercomplex
Map data
Composite map
Sample
Complex: TOC-TIC supercomplex
Protein or peptide: x 15 types
Ligand: x 4 types
Function / homology
Function and homology information
protein import into chloroplast stroma / protein targeting to chloroplast / chloroplast inner membrane / chloroplast outer membrane / chloroplast membrane / chloroplast organization / axoneme assembly / motile cilium / spermatid development / Hydrolases; Acting on acid anhydrides; Acting on GTP to facilitate cellular and subcellular movement ...protein import into chloroplast stroma / protein targeting to chloroplast / chloroplast inner membrane / chloroplast outer membrane / chloroplast membrane / chloroplast organization / axoneme assembly / motile cilium / spermatid development / Hydrolases; Acting on acid anhydrides; Acting on GTP to facilitate cellular and subcellular movement / protein transport / membrane => GO:0016020 / hydrolase activity / GTP binding / membrane / nucleus / metal ion binding / cytosol Similarity search - Function
CCB3/YggT / Chloroplast protein import component Tic20 / Protein TIC56 / YGGT family / Chloroplast import apparatus Tic20-like / GYF domain 2 / GYF domain 2 / GTPase GIMA/IAN/Toc / AIG1-type guanine nucleotide-binding (G) domain / AIG1 family ...CCB3/YggT / Chloroplast protein import component Tic20 / Protein TIC56 / YGGT family / Chloroplast import apparatus Tic20-like / GYF domain 2 / GYF domain 2 / GTPase GIMA/IAN/Toc / AIG1-type guanine nucleotide-binding (G) domain / AIG1 family / AIG1-type G domain profile. / Possible plasma membrane-binding motif in junctophilins, PIP-5-kinases and protein kinases. / MORN motif / MORN repeat / Surface antigen D15-like / Bacterial surface antigen (D15) / Omp85 superfamily domain / P-loop containing nucleoside triphosphate hydrolase Similarity search - Domain/homology
AIG1-type G domain-containing protein / Flagellar associated protein / Uncharacterized protein / J domain-containing protein / YggT family protein / YtxH domain-containing protein / Translocase of chloroplast 34 homolog, chloroplastic / Bacterial surface antigen (D15) domain-containing protein / Protein TIC 20 / Uncharacterized protein ...AIG1-type G domain-containing protein / Flagellar associated protein / Uncharacterized protein / J domain-containing protein / YggT family protein / YtxH domain-containing protein / Translocase of chloroplast 34 homolog, chloroplastic / Bacterial surface antigen (D15) domain-containing protein / Protein TIC 20 / Uncharacterized protein / Uncharacterized protein / Uncharacterized protein / Uncharacterized protein / Uncharacterized membrane protein ycf78 Similarity search - Component
Biological species
Chlamydomonas reinhardtii (plant)
Method
single particle reconstruction / cryo EM / Resolution: 2.5 Å
Journal: Cell / Year: 2022 Title: Structure of a TOC-TIC supercomplex spanning two chloroplast envelope membranes. Authors: Zeyu Jin / Li Wan / Yuqi Zhang / Xuecheng Li / Yong Cao / Haobin Liu / Shengyao Fan / Du Cao / Zhengmao Wang / Xiaobo Li / Junmin Pan / Meng-Qiu Dong / Jianping Wu / Zhen Yan / Abstract: The TOC and TIC complexes are essential translocons that facilitate the import of the nuclear genome-encoded preproteins across the two envelope membranes of chloroplast, but their exact molecular ...The TOC and TIC complexes are essential translocons that facilitate the import of the nuclear genome-encoded preproteins across the two envelope membranes of chloroplast, but their exact molecular identities and assembly remain unclear. Here, we report a cryoelectron microscopy structure of TOC-TIC supercomplex from Chlamydomonas, containing a total of 14 identified components. The preprotein-conducting pore of TOC is a hybrid β-barrel co-assembled by Toc120 and Toc75, while the potential translocation path of TIC is formed by transmembrane helices from Tic20 and YlmG, rather than a classic model of Tic110. A rigid intermembrane space (IMS) scaffold bridges two chloroplast membranes, and a large hydrophilic cleft on the IMS scaffold connects TOC and TIC, forming a pathway for preprotein translocation. Our study provides structural insights into the TOC-TIC supercomplex composition, assembly, and preprotein translocation mechanism, and lays a foundation to interpret the evolutionary conservation and diversity of this fundamental translocon machinery.
Name: Toc34 / type: protein_or_peptide / ID: 6 / Number of copies: 1 / Enantiomer: LEVO EC number: Hydrolases; Acting on acid anhydrides; Acting on GTP to facilitate cellular and subcellular movement
Model: Quantifoil R1.2/1.3 / Material: COPPER / Mesh: 300 / Support film - Material: CARBON / Support film - topology: CONTINUOUS / Support film - Film thickness: 2.0 nm
Vitrification
Cryogen name: ETHANE / Chamber humidity: 100 % / Chamber temperature: 281 K / Instrument: FEI VITROBOT MARK IV
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Electron microscopy
Microscope
FEI TITAN KRIOS
Image recording
Film or detector model: GATAN K3 (6k x 4k) / Average electron dose: 50.0 e/Å2
Electron beam
Acceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
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