登録情報 データベース : EMDB / ID : EMD-31076 構造の表示 ダウンロードとリンクタイトル Structure of the CatSpermasome マップデータMap1, overall map, 2.9A 詳細 試料複合体 : mouse CatSpermasomeリガンド : x 3種 詳細機能・相同性 機能・相同性情報分子機能 ドメイン・相同性 構成要素
CatSper complex / Sperm Motility And Taxes / sodium-independent organic anion transmembrane transporter activity / sodium-independent organic anion transport / fusion of sperm to egg plasma membrane involved in single fertilization / sperm principal piece / regulation of cilium beat frequency involved in ciliary motility / calcium ion sensor activity / flagellated sperm motility / multicellular organism development ... CatSper complex / Sperm Motility And Taxes / sodium-independent organic anion transmembrane transporter activity / sodium-independent organic anion transport / fusion of sperm to egg plasma membrane involved in single fertilization / sperm principal piece / regulation of cilium beat frequency involved in ciliary motility / calcium ion sensor activity / flagellated sperm motility / multicellular organism development / male meiotic nuclear division / organic anion transport / voltage-gated monoatomic ion channel activity / organic anion transmembrane transporter activity / calcium-activated cation channel activity / fertilization / motile cilium / regulation of monoatomic ion transmembrane transport / monoatomic ion channel complex / sperm capacitation / sodium ion transport / plasma membrane => GO:0005886 / regulation of calcium ion transport / voltage-gated calcium channel activity / sperm flagellum / bioluminescence / acrosomal vesicle / generation of precursor metabolites and energy / calcium channel activity / cilium / calcium ion transport / spermatogenesis / cell differentiation / calmodulin binding / calcium ion binding / endoplasmic reticulum / plasma membrane / cytoplasm 類似検索 - 分子機能 Cation channel sperm-associated auxiliary subunit TMEM249 / Cation channel sperm-associated auxiliary subunit gamma / Cation channel sperm-associated protein 4 / Cation channel sperm-associated protein 1 / Cation channel sperm-associated protein 2 / Cation channel sperm-associated auxiliary subunit beta / Cation channel sperm-associated auxiliary subunit delta/epsilon / Cation channel sperm-associated auxiliary subunit zeta / Cation channel sperm-associated auxiliary subunit TMEM262 / EF-hand calcium-binding domain-containing protein 9 ... Cation channel sperm-associated auxiliary subunit TMEM249 / Cation channel sperm-associated auxiliary subunit gamma / Cation channel sperm-associated protein 4 / Cation channel sperm-associated protein 1 / Cation channel sperm-associated protein 2 / Cation channel sperm-associated auxiliary subunit beta / Cation channel sperm-associated auxiliary subunit delta/epsilon / Cation channel sperm-associated auxiliary subunit zeta / Cation channel sperm-associated auxiliary subunit TMEM262 / EF-hand calcium-binding domain-containing protein 9 / CATSPERD, beta-propeller domain / CATSPERG beta-propeller domain / CATSPERB, C-terminal domain / Cation channel sperm-associated auxiliary subunit TMEM249 / Organic anion transporter polypeptide / Organic Anion Transporter Polypeptide (OATP) family / Kazal-type serine protease inhibitor domain / Kazal domain superfamily / Kazal domain / Kazal domain profile. / MFS transporter superfamily / Voltage-dependent channel domain superfamily / Green fluorescent protein, GFP / Green fluorescent protein-related / Green fluorescent protein / Green fluorescent protein / EF-hand calcium-binding domain profile. / EF-hand domain / Ion transport domain / Ion transport protein / EF-hand domain pair 類似検索 - ドメイン・相同性 Transmembrane protein 249 / Cation channel sperm-associated protein 2 / Cation channel sperm-associated protein subunit beta / Enhanced green fluorescent protein / Cation channel sperm-associated protein subunit gamma 2 / Transmembrane protein 262 / Cation channel sperm-associated protein subunit delta / Cation channel sperm-associated protein subunit epsilon / Kazal-like domain-containing protein / Cation channel sperm-associated protein 3 ... Transmembrane protein 249 / Cation channel sperm-associated protein 2 / Cation channel sperm-associated protein subunit beta / Enhanced green fluorescent protein / Cation channel sperm-associated protein subunit gamma 2 / Transmembrane protein 262 / Cation channel sperm-associated protein subunit delta / Cation channel sperm-associated protein subunit epsilon / Kazal-like domain-containing protein / Cation channel sperm-associated protein 3 / Cation channel sperm-associated protein 4 / Cation channel sperm-associated protein 1 / Cation channel sperm-associated protein subunit zeta / EF-hand calcium-binding domain-containing protein 9 類似検索 - 構成要素生物種 Mus musculus (ハツカネズミ) / Mouse (ネズミ)手法 単粒子再構成法 / クライオ電子顕微鏡法 / 解像度 : 2.9 Å 詳細 データ登録者Wu JP / Ke M 引用ジャーナル : Nature / 年 : 2021タイトル : Structure of a mammalian sperm cation channel complex.著者 : Shiyi Lin / Meng Ke / Yuqi Zhang / Zhen Yan / Jianping Wu / 要旨 : The cation channel of sperm (CatSper) is essential for sperm motility and fertility. CatSper comprises the pore-forming proteins CATSPER1-4 and multiple auxiliary subunits, including CATSPERβ, γ, ... The cation channel of sperm (CatSper) is essential for sperm motility and fertility. CatSper comprises the pore-forming proteins CATSPER1-4 and multiple auxiliary subunits, including CATSPERβ, γ, δ, ε, ζ, and EFCAB9. Here we report the cryo-electron microscopy (cryo-EM) structure of the CatSper complex isolated from mouse sperm. In the extracellular view, CATSPER1-4 conform to the conventional domain-swapped voltage-gated ion channel fold, following a counterclockwise arrangement. The auxiliary subunits CATSPERβ, γ, δ and ε-each of which contains a single transmembrane segment and a large extracellular domain-constitute a pavilion-like structure that stabilizes the entire complex through interactions with CATSPER4, 1, 3 and 2, respectively. Our EM map reveals several previously uncharacterized components, exemplified by the organic anion transporter SLCO6C1. We name this channel-transporter ultracomplex the CatSpermasome. The assembly and organization details of the CatSpermasome presented here lay the foundation for the development of CatSpermasome-related treatments for male infertility and non-hormonal contraceptives. 履歴 登録 2021年3月18日 - ヘッダ(付随情報) 公開 2021年7月28日 - マップ公開 2021年7月28日 - 更新 2021年8月11日 - 現状 2021年8月11日 処理サイト : PDBj / 状態 : 公開
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