+データを開く
-基本情報
登録情報 | データベース: EMDB / ID: EMD-30677 | |||||||||
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タイトル | Activity optimized complex I (open form) | |||||||||
マップデータ | ||||||||||
試料 |
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機能・相同性 | 機能・相同性情報 Complex I biogenesis / Mitochondrial protein import / RHOG GTPase cycle / cellular response to oxygen levels / ubiquinone-6 biosynthetic process / Respiratory electron transport / mitochondrial large ribosomal subunit binding / gliogenesis / neural precursor cell proliferation / : ...Complex I biogenesis / Mitochondrial protein import / RHOG GTPase cycle / cellular response to oxygen levels / ubiquinone-6 biosynthetic process / Respiratory electron transport / mitochondrial large ribosomal subunit binding / gliogenesis / neural precursor cell proliferation / : / [2Fe-2S] cluster assembly / oxygen sensor activity / cellular respiration / Neutrophil degranulation / respiratory chain complex I / mitochondrial ribosome / mitochondrial translation / ubiquinone binding / Mitochondrial protein degradation / NADH:ubiquinone reductase (H+-translocating) / NADH dehydrogenase activity / apoptotic mitochondrial changes / : / mitochondrial ATP synthesis coupled electron transport / mitochondrial electron transport, NADH to ubiquinone / mitochondrial respiratory chain complex I assembly / electron transport coupled proton transport / acyl binding / acyl carrier activity / NADH dehydrogenase (ubiquinone) activity / quinone binding / ATP synthesis coupled electron transport / negative regulation of intrinsic apoptotic signaling pathway / ATP metabolic process / response to cAMP / aerobic respiration / neurogenesis / respiratory electron transport chain / reactive oxygen species metabolic process / regulation of mitochondrial membrane potential / fatty acid binding / apoptotic signaling pathway / mitochondrial membrane / electron transport chain / brain development / regulation of protein phosphorylation / negative regulation of cell growth / mitochondrial intermembrane space / 2 iron, 2 sulfur cluster binding / fatty acid biosynthetic process / NAD binding / positive regulation of fibroblast proliferation / FMN binding / 4 iron, 4 sulfur cluster binding / response to oxidative stress / mitochondrial inner membrane / oxidoreductase activity / mitochondrial matrix / structural constituent of ribosome / negative regulation of DNA-templated transcription / ubiquitin protein ligase binding / protein-containing complex binding / mitochondrion / nucleoplasm / metal ion binding / cytoplasm 類似検索 - 分子機能 | |||||||||
生物種 | Bos taurus (ウシ) / Bovine (ウシ) | |||||||||
手法 | 単粒子再構成法 / クライオ電子顕微鏡法 / 解像度: 4.2 Å | |||||||||
データ登録者 | Jeon TJ / Lee SG / Yoo SH / Ryu JH / Kim DS / Hyun JK / Kim HM / Ryu SE | |||||||||
引用 | ジャーナル: Antioxid Redox Signal / 年: 2022 タイトル: A Dynamic Substrate Pool Revealed by cryo-EM of a Lipid-Preserved Respiratory Supercomplex. 著者: Tae Jin Jeon / Seong-Gyu Lee / Suk Hyun Yoo / Myeongbin Kim / Dabin Song / Joonghyun Ryu / Hwangseo Park / Deok-Soo Kim / Jaekyung Hyun / Ho Min Kim / Seong Eon Ryu / 要旨: Mitochondrial respiratory supercomplexes mediate redox electron transfer, generating a proton gradient for ATP synthesis. To provide structural information on the function of supercomplexes in ... Mitochondrial respiratory supercomplexes mediate redox electron transfer, generating a proton gradient for ATP synthesis. To provide structural information on the function of supercomplexes in physiologically relevant conditions, we conducted cryoelectron microscopy studies with supercomplexes in a lipid-preserving state. Here, we present cryoelectron microscopy structures of bovine respiratory supercomplex IIIIIV by using a lipid-preserving sample preparation. The preparation greatly enhances the intercomplex quinone transfer activity. The structures reveal large intercomplex motions that result in different shapes and sizes of the intercomplex space between complexes I and III, forming a dynamic substrate pool. Biochemical and structural analyses indicated that intercomplex phospholipids mediate the intercomplex motions. An analysis of the different classes of focus-refined complex I showed that structural switches due to quinone reduction led to the formation of a novel channel that could transfer reduced quinones to the intercomplex substrate pool. Our results indicate potential mechanism for the facilitated electron transfer involving a dynamic substrate pool and intercomplex movement by which supercomplexes play an active role in the regulation of metabolic flux and reactive oxygen species. | |||||||||
履歴 |
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-構造の表示
添付画像 |
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-ダウンロードとリンク
-EMDBアーカイブ
マップデータ | emd_30677.map.gz | 6.7 MB | EMDBマップデータ形式 | |
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ヘッダ (付随情報) | emd-30677-v30.xml emd-30677.xml | 55 KB 55 KB | 表示 表示 | EMDBヘッダ |
画像 | emd_30677.png | 114.8 KB | ||
アーカイブディレクトリ | http://ftp.pdbj.org/pub/emdb/structures/EMD-30677 ftp://ftp.pdbj.org/pub/emdb/structures/EMD-30677 | HTTPS FTP |
-検証レポート
文書・要旨 | emd_30677_validation.pdf.gz | 333 KB | 表示 | EMDB検証レポート |
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文書・詳細版 | emd_30677_full_validation.pdf.gz | 332.6 KB | 表示 | |
XML形式データ | emd_30677_validation.xml.gz | 6.1 KB | 表示 | |
CIF形式データ | emd_30677_validation.cif.gz | 7 KB | 表示 | |
アーカイブディレクトリ | https://ftp.pdbj.org/pub/emdb/validation_reports/EMD-30677 ftp://ftp.pdbj.org/pub/emdb/validation_reports/EMD-30677 | HTTPS FTP |
-関連構造データ
-リンク
EMDBのページ | EMDB (EBI/PDBe) / EMDataResource |
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「今月の分子」の関連する項目 |
-マップ
ファイル | ダウンロード / ファイル: emd_30677.map.gz / 形式: CCP4 / 大きさ: 83.7 MB / タイプ: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) | ||||||||||||||||||||||||||||||||||||
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投影像・断面図 | 画像のコントロール
画像は Spider により作成 | ||||||||||||||||||||||||||||||||||||
ボクセルのサイズ | X=Y=Z: 1.3973 Å | ||||||||||||||||||||||||||||||||||||
密度 |
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対称性 | 空間群: 1 | ||||||||||||||||||||||||||||||||||||
詳細 | EMDB XML:
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-添付データ
-試料の構成要素
+全体 : supercomplex of electron transport chain complexes
+超分子 #1: supercomplex of electron transport chain complexes
+分子 #1: NADH-ubiquinone oxidoreductase chain 2
+分子 #2: NADH-ubiquinone oxidoreductase chain 3
+分子 #3: NADH-ubiquinone oxidoreductase chain 4
+分子 #4: NADH-ubiquinone oxidoreductase chain 4L
+分子 #5: NADH-ubiquinone oxidoreductase chain 6
+分子 #6: NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial
+分子 #7: NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial
+分子 #8: NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial
+分子 #9: NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial
+分子 #10: NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial
+分子 #11: NADH dehydrogenase [ubiquinone] iron-sulfur protein 7, mitochondrial
+分子 #12: NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial
+分子 #13: NADH dehydrogenase [ubiquinone] flavoprotein 3, mitochondrial
+分子 #14: NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial
+分子 #15: NADH dehydrogenase [ubiquinone] iron-sulfur protein 5
+分子 #16: NADH dehydrogenase [ubiquinone] iron-sulfur protein 6, mitochondrial
+分子 #17: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1
+分子 #18: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2
+分子 #19: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 3
+分子 #20: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5
+分子 #21: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 6
+分子 #22: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 7
+分子 #23: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8
+分子 #24: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 9, mit...
+分子 #25: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 10, mi...
+分子 #26: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 11
+分子 #27: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12
+分子 #28: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13
+分子 #29: Acyl carrier protein, mitochondrial
+分子 #30: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 1
+分子 #31: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 2, mito...
+分子 #32: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3
+分子 #33: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4
+分子 #34: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 5, mito...
+分子 #35: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 6
+分子 #36: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7
+分子 #37: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9
+分子 #38: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 11, mit...
+分子 #39: NADH dehydrogenase [ubiquinone] 1 subunit C1, mitochondrial
+分子 #40: NADH dehydrogenase [ubiquinone] 1 subunit C2
+分子 #41: NADH-ubiquinone oxidoreductase chain 1
+分子 #42: NADH-ubiquinone oxidoreductase chain 5
+分子 #43: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10
+分子 #44: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mito...
+分子 #45: 1,2-Distearoyl-sn-glycerophosphoethanolamine
+分子 #46: CARDIOLIPIN
+分子 #47: FLAVIN MONONUCLEOTIDE
+分子 #48: IRON/SULFUR CLUSTER
+分子 #49: FE2/S2 (INORGANIC) CLUSTER
+分子 #50: ZINC ION
+分子 #51: NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE
+分子 #52: 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE
-実験情報
-構造解析
手法 | クライオ電子顕微鏡法 |
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解析 | 単粒子再構成法 |
試料の集合状態 | tissue |
-試料調製
緩衝液 | pH: 7.4 |
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凍結 | 凍結剤: ETHANE / チャンバー内湿度: 95 % / チャンバー内温度: 277 K / 装置: FEI VITROBOT MARK IV |
-電子顕微鏡法
顕微鏡 | FEI TITAN KRIOS |
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撮影 | フィルム・検出器のモデル: FEI FALCON II (4k x 4k) 平均電子線量: 35.0 e/Å2 |
電子線 | 加速電圧: 300 kV / 電子線源: FIELD EMISSION GUN |
電子光学系 | 照射モード: FLOOD BEAM / 撮影モード: BRIGHT FIELD |
実験機器 | モデル: Titan Krios / 画像提供: FEI Company |
-画像解析
CTF補正 | ソフトウェア - 名称: CTFFIND (ver. 1.4) |
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最終 再構成 | 想定した対称性 - 点群: C1 (非対称) / 解像度のタイプ: BY AUTHOR / 解像度: 4.2 Å / 解像度の算出法: FSC 0.143 CUT-OFF / ソフトウェア - 名称: RELION (ver. 2.0) / 使用した粒子像数: 72302 |
初期 角度割当 | タイプ: NOT APPLICABLE / ソフトウェア - 名称: RELION (ver. 2.0) |
最終 角度割当 | タイプ: NOT APPLICABLE / ソフトウェア - 名称: RELION (ver. 2.0) |