- EMDB-27155: Human mitochondrial DNA polymerase gamma ternary complex with GT ... -
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基本情報
登録情報
データベース: EMDB / ID: EMD-27155
タイトル
Human mitochondrial DNA polymerase gamma ternary complex with GT basepair in replication conformer
マップデータ
試料
複合体: Human mitochondrial DNA polymerase gamma ternary complex with GC basepair
タンパク質・ペプチド: DNA polymerase subunit gamma-1DNAポリメラーゼ
タンパク質・ペプチド: DNA polymerase subunit gamma-2, mitochondrialDNAポリメラーゼ
DNA: DNA (5'-D(P*AP*AP*AP*AP*CP*GP*AP*CP*GP*GP*CP*CP*AP*GP*TP*GP*CP*CP*AP*TP*AP*C)-3')
DNA: DNA (25-MER)
リガンド: CALCIUM IONカルシウム
リガンド: 2'-DEOXYCYTIDINE-5'-TRIPHOSPHATE
キーワード
DNA-binding protein (DNA結合タンパク質) / DNA polymerase (DNAポリメラーゼ) / TRANSFERASE-DNA complex
機能・相同性
機能・相同性情報
gamma DNA polymerase complex / mitochondrial DNA replication / positive regulation of DNA-directed DNA polymerase activity / single-stranded DNA 3'-5' DNA exonuclease activity / DNA replication proofreading / 加水分解酵素; エステル加水分解酵素; 5'-リン酸モノエステル産生エンドデオキシリボヌクレアーゼ / DNA metabolic process / DNA polymerase processivity factor activity / mitochondrial nucleoid / 付加脱離酵素(リアーゼ); 炭素-酸素リアーゼ類; その他の炭素-酸素リアーゼ ...gamma DNA polymerase complex / mitochondrial DNA replication / positive regulation of DNA-directed DNA polymerase activity / single-stranded DNA 3'-5' DNA exonuclease activity / DNA replication proofreading / 加水分解酵素; エステル加水分解酵素; 5'-リン酸モノエステル産生エンドデオキシリボヌクレアーゼ / DNA metabolic process / DNA polymerase processivity factor activity / mitochondrial nucleoid / 付加脱離酵素(リアーゼ); 炭素-酸素リアーゼ類; その他の炭素-酸素リアーゼ / 5'-deoxyribose-5-phosphate lyase activity / DNA polymerase binding / 3'-5' exonuclease activity / base-excision repair, gap-filling / Transcriptional activation of mitochondrial biogenesis / DNA-templated DNA replication / double-stranded DNA binding / in utero embryonic development / protease binding / DNAポリメラーゼ / DNA-directed DNA polymerase activity / ミトコンドリアマトリックス / intracellular membrane-bounded organelle / chromatin binding / protein-containing complex / ミトコンドリア / DNA binding / identical protein binding / 細胞質 類似検索 - 分子機能
DNA-directed DNA-polymerase, family A, mitochondria / DNA mitochondrial polymerase, exonuclease domain / : / DNA mitochondrial polymerase exonuclease domain / POLG2, C-terminal / Glycyl-tRNA synthetase/DNA polymerase subunit gamma-2 / Anticodon-binding / Anticodon binding domain / DNA-directed DNA polymerase, family A, conserved site / DNA polymerase family A signature. ...DNA-directed DNA-polymerase, family A, mitochondria / DNA mitochondrial polymerase, exonuclease domain / : / DNA mitochondrial polymerase exonuclease domain / POLG2, C-terminal / Glycyl-tRNA synthetase/DNA polymerase subunit gamma-2 / Anticodon-binding / Anticodon binding domain / DNA-directed DNA polymerase, family A, conserved site / DNA polymerase family A signature. / DNA-directed DNA polymerase, family A, palm domain / DNA polymerase A domain / Anticodon-binding domain superfamily / Class II Aminoacyl-tRNA synthetase/Biotinyl protein ligase (BPL) and lipoyl protein ligase (LPL) / Ribonuclease H-like superfamily / DNA/RNA polymerase superfamily 類似検索 - ドメイン・相同性
DNA polymerase subunit gamma-1 / DNA polymerase subunit gamma-2 類似検索 - 構成要素
National Institutes of Health/National Institute Of Allergy and Infectious Diseases (NIH/NIAID)
AI134611
米国
引用
ジャーナル: Nat Struct Mol Biol / 年: 2023 タイトル: Polγ coordinates DNA synthesis and proofreading to ensure mitochondrial genome integrity. 著者: Joon Park / Geoffrey K Herrmann / Patrick G Mitchell / Michael B Sherman / Y Whitney Yin / 要旨: Accurate replication of mitochondrial DNA (mtDNA) by DNA polymerase γ (Polγ) is essential for maintaining cellular energy supplies, metabolism, and cell cycle control. To illustrate the structural ...Accurate replication of mitochondrial DNA (mtDNA) by DNA polymerase γ (Polγ) is essential for maintaining cellular energy supplies, metabolism, and cell cycle control. To illustrate the structural mechanism for Polγ coordinating polymerase (pol) and exonuclease (exo) activities to ensure rapid and accurate DNA synthesis, we determined four cryo-EM structures of Polγ captured after accurate or erroneous incorporation to a resolution of 2.4-3.0 Å. The structures show that Polγ employs a dual-checkpoint mechanism to sense nucleotide misincorporation and initiate proofreading. The transition from replication to error editing is accompanied by increased dynamics in both DNA and enzyme, in which the polymerase relaxes its processivity and the primer-template DNA unwinds, rotates, and backtracks to shuttle the mismatch-containing primer terminus 32 Å to the exo site for editing. Our structural and functional studies also provide a foundation for analyses of Polγ mutation-induced human diseases and aging.