+Open data
-Basic information
Entry | Database: EMDB / ID: EMD-26606 | |||||||||
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Title | H1 Solomon Islands 2006 hemagglutinin in complex with Ab36 | |||||||||
Map data | Hemagglutinin H1 Solomon Islands in complex with Ab36 | |||||||||
Sample |
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Function / homology | Function and homology information viral budding from plasma membrane / clathrin-dependent endocytosis of virus by host cell / host cell surface receptor binding / apical plasma membrane / fusion of virus membrane with host plasma membrane / fusion of virus membrane with host endosome membrane / viral envelope / virion attachment to host cell / host cell plasma membrane / virion membrane Similarity search - Function | |||||||||
Biological species | Influenza A virus (A/Solomon Islands/3/2006(H1N1)) / Mus musculus (house mouse) | |||||||||
Method | single particle reconstruction / cryo EM / Resolution: 3.6 Å | |||||||||
Authors | Windsor IW / Caradonna TM / Schmidt AG | |||||||||
Funding support | United States, 2 items
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Citation | Journal: To Be Published Title: H1 Solomon Islands 2006 hemagglutinin in complex with Ab36 Authors: Carradona T | |||||||||
History |
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-Structure visualization
Supplemental images |
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-Downloads & links
-EMDB archive
Map data | emd_26606.map.gz | 59.5 MB | EMDB map data format | |
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Header (meta data) | emd-26606-v30.xml emd-26606.xml | 12.5 KB 12.5 KB | Display Display | EMDB header |
FSC (resolution estimation) | emd_26606_fsc.xml | 9.2 KB | Display | FSC data file |
Images | emd_26606.png | 122.7 KB | ||
Archive directory | http://ftp.pdbj.org/pub/emdb/structures/EMD-26606 ftp://ftp.pdbj.org/pub/emdb/structures/EMD-26606 | HTTPS FTP |
-Validation report
Summary document | emd_26606_validation.pdf.gz | 573.6 KB | Display | EMDB validaton report |
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Full document | emd_26606_full_validation.pdf.gz | 573.2 KB | Display | |
Data in XML | emd_26606_validation.xml.gz | 10.8 KB | Display | |
Data in CIF | emd_26606_validation.cif.gz | 14.1 KB | Display | |
Arichive directory | https://ftp.pdbj.org/pub/emdb/validation_reports/EMD-26606 ftp://ftp.pdbj.org/pub/emdb/validation_reports/EMD-26606 | HTTPS FTP |
-Related structure data
Related structure data | 7umnMC M: atomic model generated by this map C: citing same article (ref.) |
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Similar structure data | Similarity search - Function & homologyF&H Search |
-Links
EMDB pages | EMDB (EBI/PDBe) / EMDataResource |
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Related items in Molecule of the Month |
-Map
File | Download / File: emd_26606.map.gz / Format: CCP4 / Size: 64 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) | ||||||||||||||||||||||||||||||||||||
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Annotation | Hemagglutinin H1 Solomon Islands in complex with Ab36 | ||||||||||||||||||||||||||||||||||||
Projections & slices | Image control
Images are generated by Spider. | ||||||||||||||||||||||||||||||||||||
Voxel size | X=Y=Z: 0.825 Å | ||||||||||||||||||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||||||||||||||||||
Details | EMDB XML:
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-Supplemental data
-Sample components
-Entire : hemagglutinin H1 Solomon Islands/03/2006 in complex with ab109 Fab
Entire | Name: hemagglutinin H1 Solomon Islands/03/2006 in complex with ab109 Fab |
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Components |
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-Supramolecule #1: hemagglutinin H1 Solomon Islands/03/2006 in complex with ab109 Fab
Supramolecule | Name: hemagglutinin H1 Solomon Islands/03/2006 in complex with ab109 Fab type: complex / ID: 1 / Chimera: Yes / Parent: 0 / Macromolecule list: all |
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-Supramolecule #2: Hemagglutinin
Supramolecule | Name: Hemagglutinin / type: complex / ID: 2 / Chimera: Yes / Parent: 1 / Macromolecule list: #1 |
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Source (natural) | Organism: Influenza A virus (A/Solomon Islands/3/2006(H1N1)) |
-Supramolecule #3: Ab36 Fab heavy chain, Ab36 Fab light chain
Supramolecule | Name: Ab36 Fab heavy chain, Ab36 Fab light chain / type: complex / ID: 3 / Chimera: Yes / Parent: 1 / Macromolecule list: #2-#3 |
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Source (natural) | Organism: Mus musculus (house mouse) |
-Macromolecule #1: Hemagglutinin
Macromolecule | Name: Hemagglutinin / type: protein_or_peptide / ID: 1 / Number of copies: 3 / Enantiomer: LEVO |
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Source (natural) | Organism: Influenza A virus (A/Solomon Islands/3/2006(H1N1)) Strain: A/Solomon Islands/3/2006(H1N1) |
Molecular weight | Theoretical: 58.608316 KDa |
Recombinant expression | Organism: Homo sapiens (human) |
Sequence | String: ADPGYLLEDT ICIGYHANNS TDTVDTVLEK NVTVTHSVNL LEDSHNGKLC LLKGIAPLQL GNCSVAGWIL GNPECELLIS RESWSYIVE KPNPENGTCY PGHFADYEEL REQLSSVSSF ERFEIFPKES SWPNHTTTGV SASCSHNGES SFYKNLLWLT G KNGLYPNL ...String: ADPGYLLEDT ICIGYHANNS TDTVDTVLEK NVTVTHSVNL LEDSHNGKLC LLKGIAPLQL GNCSVAGWIL GNPECELLIS RESWSYIVE KPNPENGTCY PGHFADYEEL REQLSSVSSF ERFEIFPKES SWPNHTTTGV SASCSHNGES SFYKNLLWLT G KNGLYPNL SKSYANNKEK EVLVLWGVHH PPNIGDQRAL YHTENAYVSV VSSHYSRKFT PEIAKRPKVR DQEGRINYYW TL LEPGDTI IFEANGNLIA PRYAFALSRG FGSGIINSNA PMDECDAKCQ TPQGAINSSL PFQNVHPVTI GECPKYVRSA KLR MVTGLR NIPSIQSRGL FGAIAGFIEG GWTGMVDGWY GYHHQNEQGS GYAADQKSTQ NAINGITNKV NSVIEKMNTQ FTAV GKEFN KLERRMENLN KKVDDGFIDI WTYNAELLVL LENERTLDFH DSNVKNLYEK VKSQLKNNAK EIGNGCFEFY HKCND ECME SVKNGTYDYP KYSEESKLNR EKIDGVRSGS GGALEVLFQ |
-Macromolecule #2: Ab36 Fab heavy chain
Macromolecule | Name: Ab36 Fab heavy chain / type: protein_or_peptide / ID: 2 / Number of copies: 3 / Enantiomer: LEVO |
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Source (natural) | Organism: Mus musculus (house mouse) |
Molecular weight | Theoretical: 25.284295 KDa |
Recombinant expression | Organism: Homo sapiens (human) |
Sequence | String: EFQLQQSGPE LVKPGASVKI SCKASGYSFT DYNMNWVKQS DGKSLEWIGI INPNYGIISY NQKFKGKATL TVDQSSSTAY MQLNSLTSE DSAVYYCARW GNSPYYYYYM DVWGKGTTVT VSGAKTTPPS VYPLAPGSAA QTNSMVTLGC LVKGYFPEPV T VTWNSGSL ...String: EFQLQQSGPE LVKPGASVKI SCKASGYSFT DYNMNWVKQS DGKSLEWIGI INPNYGIISY NQKFKGKATL TVDQSSSTAY MQLNSLTSE DSAVYYCARW GNSPYYYYYM DVWGKGTTVT VSGAKTTPPS VYPLAPGSAA QTNSMVTLGC LVKGYFPEPV T VTWNSGSL SSGVHTFPAV LQSDLYTLSS SVTVPSSTWP SETVTCNVAH PASSTKVDKK IVPRDCGGSG LEVLFQ |
-Macromolecule #3: Ab36 Fab light chain
Macromolecule | Name: Ab36 Fab light chain / type: protein_or_peptide / ID: 3 / Number of copies: 3 / Enantiomer: LEVO |
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Source (natural) | Organism: Mus musculus (house mouse) |
Molecular weight | Theoretical: 23.375693 KDa |
Recombinant expression | Organism: Homo sapiens (human) |
Sequence | String: ENVLTQSPAI MSASPGEKVT MTCRATSSVS SSYLHWYQQK SGASPKLWIY GTSNLASGVP ARFSGSGSGT SYSLTISTVE AEDAATYYC QQYSGYPLTF GAGTKLELKR ADGAPTVSIF PPSSEQLTSG GASVVCFLNN FYPKDINVKW KIDGSERQNG V LNSWTDQD ...String: ENVLTQSPAI MSASPGEKVT MTCRATSSVS SSYLHWYQQK SGASPKLWIY GTSNLASGVP ARFSGSGSGT SYSLTISTVE AEDAATYYC QQYSGYPLTF GAGTKLELKR ADGAPTVSIF PPSSEQLTSG GASVVCFLNN FYPKDINVKW KIDGSERQNG V LNSWTDQD SKDSTYSMSS TLTLTKDEYE RHNSYTCEAT HKTSTSPIVK SFNRNEC |
-Experimental details
-Structure determination
Method | cryo EM |
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Processing | single particle reconstruction |
Aggregation state | particle |
-Sample preparation
Buffer | pH: 7.5 |
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Vitrification | Cryogen name: ETHANE |
-Electron microscopy
Microscope | FEI TITAN KRIOS |
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Image recording | Film or detector model: GATAN K3 BIOQUANTUM (6k x 4k) / Average electron dose: 1.07 e/Å2 |
Electron beam | Acceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN |
Electron optics | Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD |
Experimental equipment | Model: Titan Krios / Image courtesy: FEI Company |