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- EMDB-25621: The Envelope Glycoprotein SIVmac239.K180S SOSIP trimer in complex... -
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Open data
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Basic information
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Title | The Envelope Glycoprotein SIVmac239.K180S SOSIP trimer in complex with 3 copies of the neutralizing antibody K11 | |||||||||
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![]() | SIV / Env / vaccine / neutralizing antibody / glycoprotein / VIRAL PROTEIN / VIRAL PROTEIN-IMMUNE SYSTEM complex | |||||||||
Function / homology | ![]() membrane fusion involved in viral entry into host cell / host cell endosome membrane / membrane => GO:0016020 / symbiont entry into host cell / viral envelope / virion attachment to host cell / host cell plasma membrane / structural molecule activity / virion membrane / plasma membrane Similarity search - Function | |||||||||
Biological species | ![]() ![]() ![]() | |||||||||
Method | single particle reconstruction / cryo EM / Resolution: 3.47 Å | |||||||||
![]() | Berndsen ZT / Ward AB | |||||||||
Funding support | ![]()
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![]() | ![]() Title: Molecular insights into antibody-mediated protection against the prototypic simian immunodeficiency virus. Authors: Fangzhu Zhao / Zachary T Berndsen / Nuria Pedreño-Lopez / Alison Burns / Joel D Allen / Shawn Barman / Wen-Hsin Lee / Srirupa Chakraborty / Sandrasegaram Gnanakaran / Leigh M Sewall / ...Authors: Fangzhu Zhao / Zachary T Berndsen / Nuria Pedreño-Lopez / Alison Burns / Joel D Allen / Shawn Barman / Wen-Hsin Lee / Srirupa Chakraborty / Sandrasegaram Gnanakaran / Leigh M Sewall / Gabriel Ozorowski / Oliver Limbo / Ge Song / Peter Yong / Sean Callaghan / Jessica Coppola / Kim L Weisgrau / Jeffrey D Lifson / Rebecca Nedellec / Thomas B Voigt / Fernanda Laurino / Johan Louw / Brandon C Rosen / Michael Ricciardi / Max Crispin / Ronald C Desrosiers / Eva G Rakasz / David I Watkins / Raiees Andrabi / Andrew B Ward / Dennis R Burton / Devin Sok / ![]() ![]() Abstract: SIVmac239 infection of macaques is a favored model of human HIV infection. However, the SIVmac239 envelope (Env) trimer structure, glycan occupancy, and the targets and ability of neutralizing ...SIVmac239 infection of macaques is a favored model of human HIV infection. However, the SIVmac239 envelope (Env) trimer structure, glycan occupancy, and the targets and ability of neutralizing antibodies (nAbs) to protect against SIVmac239 remain unknown. Here, we report the isolation of SIVmac239 nAbs that recognize a glycan hole and the V1/V4 loop. A high-resolution structure of a SIVmac239 Env trimer-nAb complex shows many similarities to HIV and SIVcpz Envs, but with distinct V4 features and an extended V1 loop. Moreover, SIVmac239 Env has a higher glycan shield density than HIV Env that may contribute to poor or delayed nAb responses in SIVmac239-infected macaques. Passive transfer of a nAb protects macaques from repeated intravenous SIVmac239 challenge at serum titers comparable to those described for protection of humans against HIV infection. Our results provide structural insights for vaccine design and shed light on antibody-mediated protection in the SIV model. | |||||||||
History |
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Structure visualization
Supplemental images |
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Downloads & links
-EMDB archive
Map data | ![]() | 97.1 MB | ![]() | |
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Header (meta data) | ![]() ![]() | 20.9 KB 20.9 KB | Display Display | ![]() |
FSC (resolution estimation) | ![]() | 10.4 KB | Display | ![]() |
Images | ![]() | 127.7 KB | ||
Masks | ![]() | 103 MB | ![]() | |
Others | ![]() ![]() | 95.4 MB 95.4 MB | ||
Archive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 7t2pMC ![]() 7t4gC M: atomic model generated by this map C: citing same article ( |
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Similar structure data | Similarity search - Function & homology ![]() |
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Links
EMDB pages | ![]() ![]() |
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Related items in Molecule of the Month |
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Map
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Voxel size | X=Y=Z: 1.15 Å | ||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||
Details | EMDB XML:
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-Supplemental data
-Mask #1
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Density Histograms |
-Half map: #1
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Density Histograms |
-Half map: #2
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Density Histograms |
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Sample components
-Entire : SIVmac239.K180S SOSIP trimer in complex with 3 copies of K11 Fab
Entire | Name: SIVmac239.K180S SOSIP trimer in complex with 3 copies of K11 Fab |
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Components |
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-Supramolecule #1: SIVmac239.K180S SOSIP trimer in complex with 3 copies of K11 Fab
Supramolecule | Name: SIVmac239.K180S SOSIP trimer in complex with 3 copies of K11 Fab type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1-#4 |
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Source (natural) | Organism: ![]() |
-Macromolecule #1: Envelope glycoprotein gp120
Macromolecule | Name: Envelope glycoprotein gp120 / type: protein_or_peptide / ID: 1 / Number of copies: 1 / Enantiomer: LEVO |
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Source (natural) | Organism: ![]() |
Molecular weight | Theoretical: 60.324441 KDa |
Recombinant expression | Organism: ![]() |
Sequence | String: MGCLGNQLLI AILLLSVYGI YCTLYVTVFY GVPAWRNATI PLFCATKNRD TWGTTQCLPD NGDYSEMALN VTESFDAWNN TVTEQAIED VWQLFETSIK PCVKLSPLCI TMRCNKSETD RWGLTKSITT TASTTSTTAS AKVDMVNETS SCIAQDNCTG L EQEQMISC ...String: MGCLGNQLLI AILLLSVYGI YCTLYVTVFY GVPAWRNATI PLFCATKNRD TWGTTQCLPD NGDYSEMALN VTESFDAWNN TVTEQAIED VWQLFETSIK PCVKLSPLCI TMRCNKSETD RWGLTKSITT TASTTSTTAS AKVDMVNETS SCIAQDNCTG L EQEQMISC KFNMTGLKRD KSKEYNETWY SADLVCEQGN NTGNESRCYM NHCNTSVIQE SCDKHYWDAI RFRYCAPPGY AL LRCNDTN YSGFMPKCSK VVVSSCTRMM ETQTSTWFGF NGTRAENRTY IYWHGRDNRT IISLNKYYNL TMKCRRPGNK TVL PVTIMS GLVFHSQPIN DRPKQAWCWF GGKWKDAIKE VKQTIVKHPR YTGTNNTDKI NLTAPGGGDP EVTFMWTNCR GEFL YCKMN WFLNWVEDRN TANQKPKEQH KRNYVPCHIR QIINTWHKVG KNVYLPPREG DLTCNSTVTS LIANIDWIDG NQTNI TMSA EVAELYRLEL GDYKLVEITP IGLAPTSCKR YTTGGTSRRR RRR |
-Macromolecule #2: Envelope glycoprotein gp41
Macromolecule | Name: Envelope glycoprotein gp41 / type: protein_or_peptide / ID: 2 / Number of copies: 1 / Enantiomer: LEVO |
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Source (natural) | Organism: ![]() |
Molecular weight | Theoretical: 18.1446 KDa |
Recombinant expression | Organism: ![]() |
Sequence | String: GVFVLGFLGF LATAGSAMGA ASLTLTAQSR TLLAGIVQQQ QQLLDVPKRQ QELLRLTVWG TKNLQTRVTA IEKYLKDQAQ LNAWGCAFR QVCCTTVPWP NASLIPKWNN ETWQEWERKV DFLEENITAL LEEAQIQQEK NMYELQKLNG SGHHHHHHHH |
-Macromolecule #3: K11 Fab heavy chain
Macromolecule | Name: K11 Fab heavy chain / type: protein_or_peptide / ID: 3 / Number of copies: 1 / Enantiomer: LEVO |
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Source (natural) | Organism: ![]() ![]() |
Molecular weight | Theoretical: 53.29077 KDa |
Recombinant expression | Organism: ![]() |
Sequence | String: DWTWRILFLV AAATGAHSQV QLQESGPGVV RPSQTLSLTC AVSGDTVSSC CFFWTWIRQP PGKGLEWIGN ISYDNDNTNY NPSLKTRIS ISKDMSKNQF SLKLNSLTAT DTAIYYCARE SPSRGNFCYA YLYGNCPLHF DLWGQGVLVT VSSASTKGPS V FPLAPSSR ...String: DWTWRILFLV AAATGAHSQV QLQESGPGVV RPSQTLSLTC AVSGDTVSSC CFFWTWIRQP PGKGLEWIGN ISYDNDNTNY NPSLKTRIS ISKDMSKNQF SLKLNSLTAT DTAIYYCARE SPSRGNFCYA YLYGNCPLHF DLWGQGVLVT VSSASTKGPS V FPLAPSSR STSESTAALG CLVKDYFPEP VTVSWNSGSL TSGVHTFPAV LQSSGLYSLS SVVTVPSSSL GTQTYVCNVN HK PSNTKVD KRVEIKTCGG GSKPPTCPPC TSPELLGGPS VFLFPPKPKD TLMISRTPEV TCVVVDVSQE DPDVKFNWYV NGA EVHHAQ TKPRETQYNS TYRVVSVLTV THQDWLNGKE YTCKVSNKAL PAPIQKTISK DKGQPREPQV YTLPPSREEL TKNQ VSLTC LVKGFYPSDI VVEWESSGQP ENTYKTTPPV LDSDGSYFLY SKLTVDKSRW QQGNVFSCSV MHEALHNHYT QKSLS LSPG K |
-Macromolecule #4: K11 Fab light chain
Macromolecule | Name: K11 Fab light chain / type: protein_or_peptide / ID: 4 / Number of copies: 1 / Enantiomer: LEVO |
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Source (natural) | Organism: ![]() ![]() |
Molecular weight | Theoretical: 25.29315 KDa |
Recombinant expression | Organism: ![]() |
Sequence | String: ETDTLLLWVL LLWVPGSTGD ILLTQSPSSL SGSVGDRVTI TCRASQGINS YLNWYQQKPG KAPKLLIYFA NRLQSGVPSR FSGSGSGTE FTLTISSLQS EDGATYYCQQ YDTFPTFGPG TKLDIKRTVA APSVFIFPPS EDQVKSGTVS VVCLLNNFYP R EASVKWKV ...String: ETDTLLLWVL LLWVPGSTGD ILLTQSPSSL SGSVGDRVTI TCRASQGINS YLNWYQQKPG KAPKLLIYFA NRLQSGVPSR FSGSGSGTE FTLTISSLQS EDGATYYCQQ YDTFPTFGPG TKLDIKRTVA APSVFIFPPS EDQVKSGTVS VVCLLNNFYP R EASVKWKV DGALKTGNSQ ESVTEQDSKD NTYSLSSTLT LSSTEYQSHK VYACEVTHQG LSSPVTKSFN RGEC |
-Macromolecule #11: 2-acetamido-2-deoxy-beta-D-glucopyranose
Macromolecule | Name: 2-acetamido-2-deoxy-beta-D-glucopyranose / type: ligand / ID: 11 / Number of copies: 13 / Formula: NAG |
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Molecular weight | Theoretical: 221.208 Da |
Chemical component information | ![]() ChemComp-NAG: |
-Experimental details
-Structure determination
Method | cryo EM |
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![]() | single particle reconstruction |
Aggregation state | particle |
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Sample preparation
Concentration | 1 mg/mL |
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Buffer | pH: 7.4 |
Grid | Model: C-flat-1.2/1.3 / Pretreatment - Type: GLOW DISCHARGE |
Vitrification | Cryogen name: ETHANE |
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Electron microscopy
Microscope | FEI TALOS ARCTICA |
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Image recording | Film or detector model: GATAN K2 SUMMIT (4k x 4k) / Detector mode: COUNTING / Average electron dose: 50.0 e/Å2 |
Electron beam | Acceleration voltage: 200 kV / Electron source: ![]() |
Electron optics | Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD |
Experimental equipment | ![]() Model: Talos Arctica / Image courtesy: FEI Company |