ジャーナル: Proc Natl Acad Sci U S A / 年: 2014 タイトル: Neutralizing antibodies can initiate genome release from human enterovirus 71. 著者: Pavel Plevka / Pei-Yin Lim / Rushika Perera / Jane Cardosa / Ampa Suksatu / Richard J Kuhn / Michael G Rossmann / 要旨: Antibodies were prepared by immunizing mice with empty, immature particles of human enterovirus 71 (EV71), a picornavirus that causes severe neurological disease in young children. The capsid ...Antibodies were prepared by immunizing mice with empty, immature particles of human enterovirus 71 (EV71), a picornavirus that causes severe neurological disease in young children. The capsid structure of these empty particles is different from that of the mature virus and is similar to "A" particles encountered when picornaviruses recognize a potential host cell before genome release. The monoclonal antibody E18, generated by this immunization, induced a conformational change when incubated at temperatures between 4 °C and 37 °C with mature virus, transforming infectious virions into A particles. The resultant loss of genome that was observed by cryo-EM and a fluorescent SYBR Green dye assay inactivated the virus, establishing the mechanism by which the virus is inactivated and demonstrating that the E18 antibody has potential as an anti-EV71 therapy. The antibody-mediated virus neutralization by the induction of genome release has not been previously demonstrated. Furthermore, the present results indicate that antibodies with genome-release activity could also be produced for other picornaviruses by immunization with immature particles.
#20 - 2001年8月 ポリオウイルスとライノウイルス (Poliovirus and Rhinovirus) 類似性 (21)
#200 - 2016年8月 正二十面体型ウイルスの準対称性 (Quasisymmetry in Icosahedral Viruses) 類似性 (1)
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マップ
ファイル
ダウンロード / ファイル: emd_2436.map.gz / 形式: CCP4 / 大きさ: 29.8 MB / タイプ: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
注釈
Reconstruction of human enterovirus 71 with bound Fab fragments of antibody E19
ボクセルのサイズ
X=Y=Z: 2.48 Å
密度
表面レベル
登録者による: 1.0 / ムービー #1: 1
最小 - 最大
-3.7741611 - 4.40684891
平均 (標準偏差)
0.02253945 (±0.90394598)
対称性
空間群: 1
詳細
EMDB XML:
マップ形状
Axis order
X
Y
Z
Origin
-100
-100
-100
サイズ
200
200
200
Spacing
200
200
200
セル
A=B=C: 496.0 Å α=β=γ: 90.0 °
CCP4マップ ヘッダ情報:
mode
Image stored as Reals
Å/pix. X/Y/Z
2.48
2.48
2.48
M x/y/z
200
200
200
origin x/y/z
0.000
0.000
0.000
length x/y/z
496.000
496.000
496.000
α/β/γ
90.000
90.000
90.000
start NX/NY/NZ
0
0
-40
NX/NY/NZ
55
55
81
MAP C/R/S
1
2
3
start NC/NR/NS
-100
-100
-100
NC/NR/NS
200
200
200
D min/max/mean
-3.774
4.407
0.023
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添付データ
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試料の構成要素
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全体 : Reconstruction of human enterovirus 71 with bound Fab fragments o...
全体
名称: Reconstruction of human enterovirus 71 with bound Fab fragments of antibody E19
要素
試料: Reconstruction of human enterovirus 71 with bound Fab fragments of antibody E19
ウイルス: Human enterovirus 71 (エンテロウイルス)
タンパク質・ペプチド: EV19/5/C1-6/F1/C11
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超分子 #1000: Reconstruction of human enterovirus 71 with bound Fab fragments o...
超分子
名称: Reconstruction of human enterovirus 71 with bound Fab fragments of antibody E19 タイプ: sample / ID: 1000 / 集合状態: Icosahedral virus and 60 copies of bound Fab / Number unique components: 2
分子量
理論値: 6.8 MDa
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超分子 #1: Human enterovirus 71
超分子
名称: Human enterovirus 71 / タイプ: virus / ID: 1 / Name.synonym: EV71 / NCBI-ID: 39054 / 生物種: Human enterovirus 71 / ウイルスタイプ: VIRION / ウイルス・単離状態: SEROTYPE / ウイルス・エンベロープ: No / ウイルス・中空状態: Yes / Syn species name: EV71 / Sci species serotype: EV-174