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Open data
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Basic information
Entry | Database: EMDB / ID: EMD-21445 | |||||||||
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Title | Cryo-EM structure of Arabidopsis thaliana MSL1 A320V | |||||||||
![]() | Arabidopsis thaliana MSL1 A320V | |||||||||
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![]() | ion channel / TRANSPORT PROTEIN | |||||||||
Function / homology | ![]() mechanosensitive monoatomic ion channel activity / chloroplast envelope / chloroplast / cellular response to oxidative stress / mitochondrial inner membrane / mitochondrion Similarity search - Function | |||||||||
Biological species | ![]() ![]() | |||||||||
Method | single particle reconstruction / cryo EM / Resolution: 2.96 Å | |||||||||
![]() | Deng Z / Zhang J / Yuan P | |||||||||
![]() | ![]() Title: Structural mechanism for gating of a eukaryotic mechanosensitive channel of small conductance. Authors: Zengqin Deng / Grigory Maksaev / Angela M Schlegel / Jingying Zhang / Michael Rau / James A J Fitzpatrick / Elizabeth S Haswell / Peng Yuan / ![]() Abstract: Mechanosensitive ion channels transduce physical force into electrochemical signaling that underlies an array of fundamental physiological processes, including hearing, touch, proprioception, ...Mechanosensitive ion channels transduce physical force into electrochemical signaling that underlies an array of fundamental physiological processes, including hearing, touch, proprioception, osmoregulation, and morphogenesis. The mechanosensitive channels of small conductance (MscS) constitute a remarkably diverse superfamily of channels critical for management of osmotic pressure. Here, we present cryo-electron microscopy structures of a MscS homolog from Arabidopsis thaliana, MSL1, presumably in both the closed and open states. The heptameric MSL1 channel contains an unusual bowl-shaped transmembrane region, which is reminiscent of the evolutionarily and architecturally unrelated mechanosensitive Piezo channels. Upon channel opening, the curved transmembrane domain of MSL1 flattens and expands. Our structures, in combination with functional analyses, delineate a structural mechanism by which mechanosensitive channels open under increased membrane tension. Further, the shared structural feature between unrelated channels suggests the possibility of a unified mechanical gating mechanism stemming from membrane deformation induced by a non-planar transmembrane domain. | |||||||||
History |
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Structure visualization
Movie |
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Structure viewer | EM map: ![]() ![]() ![]() |
Supplemental images |
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Downloads & links
-EMDB archive
Map data | ![]() | 49 MB | ![]() | |
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Header (meta data) | ![]() ![]() | 10.2 KB 10.2 KB | Display Display | ![]() |
Images | ![]() | 161.2 KB | ||
Filedesc metadata | ![]() | 5.1 KB | ||
Archive directory | ![]() ![]() | HTTPS FTP |
-Validation report
Summary document | ![]() | 481.3 KB | Display | ![]() |
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Full document | ![]() | 480.9 KB | Display | |
Data in XML | ![]() | 6.1 KB | Display | |
Data in CIF | ![]() | 7 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 6vxnMC ![]() 6vxmC ![]() 6vxpC C: citing same article ( M: atomic model generated by this map |
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Similar structure data |
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Links
EMDB pages | ![]() ![]() |
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Related items in Molecule of the Month |
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Map
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Annotation | Arabidopsis thaliana MSL1 A320V | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Voxel size | X=Y=Z: 1.1 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Details | EMDB XML:
CCP4 map header:
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-Supplemental data
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Sample components
-Entire : MSL1 A320V
Entire | Name: MSL1 A320V |
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Components |
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-Supramolecule #1: MSL1 A320V
Supramolecule | Name: MSL1 A320V / type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1 |
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Source (natural) | Organism: ![]() ![]() |
-Macromolecule #1: Mechanosensitive ion channel protein 1, mitochondrial
Macromolecule | Name: Mechanosensitive ion channel protein 1, mitochondrial / type: protein_or_peptide / ID: 1 / Number of copies: 7 / Enantiomer: LEVO |
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Source (natural) | Organism: ![]() ![]() |
Molecular weight | Theoretical: 46.604586 KDa |
Recombinant expression | Organism: ![]() |
Sequence | String: MSSKSDDFGS IVASGVTGSG DGNGNGNDWV EKAKDVLQTS VDAVTETAKK TKDVSDEMIP HVQQFLDSNP YLKDVIVPVS LTMTGTLFA WVVMPRILRR FHTYAMQSSA KLLPVGFSNE DVPYEKSFWG ALEDPARYLV TFIAFAQIAA MVAPTTIAAQ Y FSPTVKGA ...String: MSSKSDDFGS IVASGVTGSG DGNGNGNDWV EKAKDVLQTS VDAVTETAKK TKDVSDEMIP HVQQFLDSNP YLKDVIVPVS LTMTGTLFA WVVMPRILRR FHTYAMQSSA KLLPVGFSNE DVPYEKSFWG ALEDPARYLV TFIAFAQIAA MVAPTTIAAQ Y FSPTVKGA VILSLVWFLY RWKTNVITRM LSAKSFGGLD REKVLTLDKV SSVGLFAIGL MASAEACGVA VQSILTVGGV GG VVTAFAA RDILGNVLSG LSMQFSRPFS MGDTIKAGSV EGQVIEMGLT TTSLLNAEKF PVLVPNSLFS SQVIVNKSRA QWR AIASKI PLQIDDLDMI PQISNEIKEM LRSNTKVFLG KEAPHCYLSR VEKSFAELTI GCNLIRMGKE ELYNTQQEVL LEAV KIIKK HGVSLGTTWD NSTLSNSLEV LFQ UniProtKB: Mechanosensitive ion channel protein 1, mitochondrial |
-Macromolecule #2: DODECANE
Macromolecule | Name: DODECANE / type: ligand / ID: 2 / Number of copies: 7 / Formula: D12 |
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Molecular weight | Theoretical: 170.335 Da |
Chemical component information | ![]() ChemComp-D12: |
-Experimental details
-Structure determination
Method | cryo EM |
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![]() | single particle reconstruction |
Aggregation state | particle |
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Sample preparation
Concentration | 1 mg/mL |
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Buffer | pH: 8 |
Grid | Details: unspecified |
Vitrification | Cryogen name: ETHANE |
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Electron microscopy
Microscope | FEI TITAN KRIOS |
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Image recording | #0 - Image recording ID: 1 / #0 - Film or detector model: GATAN K2 SUMMIT (4k x 4k) / #0 - Average electron dose: 62.0 e/Å2 / #1 - Image recording ID: 2 / #1 - Film or detector model: GATAN K2 SUMMIT (4k x 4k) / #1 - Average electron dose: 62.0 e/Å2 |
Electron beam | Acceleration voltage: 300 kV / Electron source: ![]() |
Electron optics | Illumination mode: OTHER / Imaging mode: OTHER |
Experimental equipment | ![]() Model: Titan Krios / Image courtesy: FEI Company |