+データを開く
-基本情報
登録情報 | データベース: EMDB / ID: EMD-21031 | |||||||||
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タイトル | Cryo-EM structure of the Acinetobacter baumannii Ribosome: 70S with E-site tRNA | |||||||||
マップデータ | Composite EM map of 70S E-site occupied ribosome from A. baumannii created by combining three separate focused refinements (50S, 30S core and 30S head) | |||||||||
試料 |
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機能・相同性 | 機能・相同性情報 ribosomal small subunit biogenesis / small ribosomal subunit rRNA binding / large ribosomal subunit / ribosomal small subunit assembly / large ribosomal subunit rRNA binding / small ribosomal subunit / 5S rRNA binding / transferase activity / cytosolic small ribosomal subunit / ribosomal large subunit assembly ...ribosomal small subunit biogenesis / small ribosomal subunit rRNA binding / large ribosomal subunit / ribosomal small subunit assembly / large ribosomal subunit rRNA binding / small ribosomal subunit / 5S rRNA binding / transferase activity / cytosolic small ribosomal subunit / ribosomal large subunit assembly / cytoplasmic translation / cytosolic large ribosomal subunit / tRNA binding / negative regulation of translation / rRNA binding / ribosome / structural constituent of ribosome / translation / ribonucleoprotein complex / mRNA binding / RNA binding / cytosol / cytoplasm 類似検索 - 分子機能 | |||||||||
生物種 | Acinetobacter baumannii AB0057 (バクテリア) / Acinetobacter sp. CIP 51.11 (バクテリア) / Acinetobacter beijerinckii ANC 3835 (バクテリア) / Acinetobacter baumannii (strain AB0057) (バクテリア) / Acinetobacter sp. ANC 4470 (バクテリア) / Acinetobacter sp. NIPH 899 (バクテリア) / Acinetobacter baumannii (バクテリア) / Acinetobacter sp. 809848 (バクテリア) / Acinetobacter rudis CIP 110305 (バクテリア) / Acinetobacter sp. CIP 102082 (バクテリア) / Acinetobacter sp. ANC 5600 (バクテリア) / Acinetobacter sp. 263903-1 (バクテリア) / Acinetobacter venetianus (バクテリア) | |||||||||
手法 | 単粒子再構成法 / クライオ電子顕微鏡法 / 解像度: 2.82 Å | |||||||||
データ登録者 | Morgan CE / Yu EW | |||||||||
資金援助 | 米国, 1件
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引用 | ジャーナル: mBio / 年: 2020 タイトル: Cryo-electron Microscopy Structure of the Acinetobacter baumannii 70S Ribosome and Implications for New Antibiotic Development. 著者: Christopher E Morgan / Wei Huang / Susan D Rudin / Derek J Taylor / James E Kirby / Robert A Bonomo / Edward W Yu / 要旨: Antimicrobial resistance is a major health threat as it limits treatment options for infection. At the forefront of this serious issue is , a Gram-negative opportunistic pathogen that exhibits the ...Antimicrobial resistance is a major health threat as it limits treatment options for infection. At the forefront of this serious issue is , a Gram-negative opportunistic pathogen that exhibits the remarkable ability to resist antibiotics through multiple mechanisms. As bacterial ribosomes represent a target for multiple distinct classes of existing antimicrobial agents, we here use single-particle cryo-electron microscopy (cryo-EM) to elucidate five different structural states of the ribosome, including the 70S, 50S, and 30S forms. We also determined interparticle motions of the 70S ribosome in different tRNA bound states using three-dimensional (3D) variability analysis. Together, our structural data further our understanding of the ribosome from and other Gram-negative pathogens and will enable structure-based drug discovery to combat antibiotic-resistant bacterial infections. is a severe nosocomial threat largely due to its intrinsic antibiotic resistance and remarkable ability to acquire new resistance determinants. The bacterial ribosome serves as a major target for modern antibiotics and the design of new therapeutics. Here, we present cryo-EM structures of the 70S ribosome, revealing several unique species-specific structural features that may facilitate future drug development to combat this recalcitrant bacterial pathogen. | |||||||||
履歴 |
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-構造の表示
ムービー |
ムービービューア |
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構造ビューア | EMマップ: SurfViewMolmilJmol/JSmol |
添付画像 |
-ダウンロードとリンク
-EMDBアーカイブ
マップデータ | emd_21031.map.gz | 14.5 MB | EMDBマップデータ形式 | |
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ヘッダ (付随情報) | emd-21031-v30.xml emd-21031.xml | 71.7 KB 71.7 KB | 表示 表示 | EMDBヘッダ |
画像 | emd_21031.png | 214.6 KB | ||
その他 | emd_21031_additional_1.map.gz emd_21031_additional_2.map.gz emd_21031_additional_3.map.gz emd_21031_additional_4.map.gz | 16.2 MB 483.8 MB 484 MB 483.5 MB | ||
アーカイブディレクトリ | http://ftp.pdbj.org/pub/emdb/structures/EMD-21031 ftp://ftp.pdbj.org/pub/emdb/structures/EMD-21031 | HTTPS FTP |
-検証レポート
文書・要旨 | emd_21031_validation.pdf.gz | 354.7 KB | 表示 | EMDB検証レポート |
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文書・詳細版 | emd_21031_full_validation.pdf.gz | 354.3 KB | 表示 | |
XML形式データ | emd_21031_validation.xml.gz | 8 KB | 表示 | |
CIF形式データ | emd_21031_validation.cif.gz | 9.2 KB | 表示 | |
アーカイブディレクトリ | https://ftp.pdbj.org/pub/emdb/validation_reports/EMD-21031 ftp://ftp.pdbj.org/pub/emdb/validation_reports/EMD-21031 | HTTPS FTP |
-関連構造データ
-リンク
EMDBのページ | EMDB (EBI/PDBe) / EMDataResource |
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「今月の分子」の関連する項目 |
-マップ
ファイル | ダウンロード / ファイル: emd_21031.map.gz / 形式: CCP4 / 大きさ: 512 MB / タイプ: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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注釈 | Composite EM map of 70S E-site occupied ribosome from A. baumannii created by combining three separate focused refinements (50S, 30S core and 30S head) | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
投影像・断面図 | 画像のコントロール
画像は Spider により作成 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
ボクセルのサイズ | X=Y=Z: 1.064 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
密度 |
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対称性 | 空間群: 1 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
詳細 | EMDB XML:
CCP4マップ ヘッダ情報:
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-添付データ
-追加マップ: Composite EM map of 70S E-site occupied ribosome...
ファイル | emd_21031_additional_1.map | ||||||||||||
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注釈 | Composite EM map of 70S E-site occupied ribosome from A. baumannii created by combining three separate focused refinements (50S, 30S core and 30S head), local resolution | ||||||||||||
投影像・断面図 |
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密度ヒストグラム |
-追加マップ: Focused refinement of 30S head of 70S E-site...
ファイル | emd_21031_additional_2.map | ||||||||||||
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注釈 | Focused refinement of 30S head of 70S E-site occupied ribosome from A. baumannii | ||||||||||||
投影像・断面図 |
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密度ヒストグラム |
-追加マップ: Focused refinement of 30S core of 70S E-site...
ファイル | emd_21031_additional_3.map | ||||||||||||
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注釈 | Focused refinement of 30S core of 70S E-site occupied ribosome from A. baumannii | ||||||||||||
投影像・断面図 |
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密度ヒストグラム |
-追加マップ: Focused refinement of 50S large subunit of 70S...
ファイル | emd_21031_additional_4.map | ||||||||||||
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注釈 | Focused refinement of 50S large subunit of 70S E-site occupied ribosome from A. baumannii | ||||||||||||
投影像・断面図 |
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密度ヒストグラム |
-試料の構成要素
+全体 : Acinetobacter baumannii 70S ribosome with E-site tRNA bound
+超分子 #1: Acinetobacter baumannii 70S ribosome with E-site tRNA bound
+分子 #1: 50S ribosomal protein L33
+分子 #2: 50S ribosomal protein L34
+分子 #3: 50S ribosomal protein L35
+分子 #4: 50S ribosomal protein L36
+分子 #7: 50S ribosomal protein L2
+分子 #8: 50S ribosomal protein L3
+分子 #9: 50S ribosomal protein L4
+分子 #10: 50S ribosomal protein L5
+分子 #11: 50S ribosomal protein L6
+分子 #12: 50S ribosomal protein L9
+分子 #13: 50S ribosomal protein L13
+分子 #14: 50S ribosomal protein L14
+分子 #15: 50S ribosomal protein L15
+分子 #16: 50S ribosomal protein L16
+分子 #17: 50S ribosomal protein L17
+分子 #18: 50S ribosomal protein L18
+分子 #19: 50S ribosomal protein L19
+分子 #20: 50S ribosomal protein L20
+分子 #21: 50S ribosomal protein L21
+分子 #22: 50S ribosomal protein L22
+分子 #23: 50S ribosomal protein L23
+分子 #24: 50S ribosomal protein L24
+分子 #25: 50S ribosomal protein L25
+分子 #26: 50S ribosomal protein L27
+分子 #27: 50S ribosomal protein L28
+分子 #28: 50S ribosomal protein L29
+分子 #29: 50S ribosomal protein L30
+分子 #30: 50S ribosomal protein L32
+分子 #32: 30S ribosomal protein S2
+分子 #33: 30S ribosomal protein S3
+分子 #34: 30S ribosomal protein S4
+分子 #35: 30S ribosomal protein S5
+分子 #36: 30S ribosomal protein S6
+分子 #37: 30S ribosomal protein S7
+分子 #38: 30S ribosomal protein S8
+分子 #39: 30S ribosomal protein S9
+分子 #40: 30S ribosomal protein S10
+分子 #41: 30S ribosomal protein S11
+分子 #42: 30S ribosomal protein S12
+分子 #43: 30S ribosomal protein S13
+分子 #44: 30S ribosomal protein S14
+分子 #45: 30S ribosomal protein S15
+分子 #46: 30S ribosomal protein S16
+分子 #47: 30S ribosomal protein S17
+分子 #48: 30S ribosomal protein S18
+分子 #49: 30S ribosomal protein S19
+分子 #50: 30S ribosomal protein S20
+分子 #51: 30S ribosomal protein S21
+分子 #5: 23s ribosomal RNA
+分子 #6: 5s ribosomal RNA
+分子 #31: 16s Ribosomal RNA
+分子 #52: tRNA-met
+分子 #53: mRNA
+分子 #54: ZINC ION
+分子 #55: MAGNESIUM ION
+分子 #56: SODIUM ION
+分子 #57: water
-実験情報
-構造解析
手法 | クライオ電子顕微鏡法 |
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解析 | 単粒子再構成法 |
試料の集合状態 | particle |
-試料調製
緩衝液 | pH: 7.6 |
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凍結 | 凍結剤: ETHANE |
-電子顕微鏡法
顕微鏡 | FEI TITAN KRIOS |
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撮影 | フィルム・検出器のモデル: GATAN K2 SUMMIT (4k x 4k) 検出モード: SUPER-RESOLUTION / 平均電子線量: 40.0 e/Å2 |
電子線 | 加速電圧: 300 kV / 電子線源: FIELD EMISSION GUN |
電子光学系 | 照射モード: FLOOD BEAM / 撮影モード: BRIGHT FIELD |
実験機器 | モデル: Titan Krios / 画像提供: FEI Company |