+データを開く
-基本情報
登録情報 | データベース: EMDB / ID: EMD-18950 | |||||||||
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タイトル | 70S Escherichia coli ribosome with Paenilamicin B2 bound with A- and P-site tRNA. | |||||||||
マップデータ | Post processed masked map | |||||||||
試料 |
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キーワード | Ribosome / 70S / Paenilamicin / Antibiotic | |||||||||
機能・相同性 | 機能・相同性情報 negative regulation of cytoplasmic translational initiation / ornithine decarboxylase inhibitor activity / transcription antitermination factor activity, RNA binding / misfolded RNA binding / Group I intron splicing / RNA folding / transcriptional attenuation / endoribonuclease inhibitor activity / RNA-binding transcription regulator activity / positive regulation of ribosome biogenesis ...negative regulation of cytoplasmic translational initiation / ornithine decarboxylase inhibitor activity / transcription antitermination factor activity, RNA binding / misfolded RNA binding / Group I intron splicing / RNA folding / transcriptional attenuation / endoribonuclease inhibitor activity / RNA-binding transcription regulator activity / positive regulation of ribosome biogenesis / negative regulation of cytoplasmic translation / four-way junction DNA binding / DnaA-L2 complex / translation repressor activity / negative regulation of DNA-templated DNA replication initiation / negative regulation of translational initiation / regulation of mRNA stability / mRNA regulatory element binding translation repressor activity / ribosome assembly / assembly of large subunit precursor of preribosome / positive regulation of RNA splicing / transcription elongation factor complex / cytosolic ribosome assembly / regulation of DNA-templated transcription elongation / DNA endonuclease activity / response to reactive oxygen species / transcription antitermination / regulation of cell growth / translational initiation / DNA-templated transcription termination / maintenance of translational fidelity / response to radiation / mRNA 5'-UTR binding / ribosomal small subunit biogenesis / small ribosomal subunit rRNA binding / large ribosomal subunit / ribosome biogenesis / ribosome binding / regulation of translation / ribosomal small subunit assembly / small ribosomal subunit / 5S rRNA binding / large ribosomal subunit rRNA binding / transferase activity / cytosolic small ribosomal subunit / ribosomal large subunit assembly / cytoplasmic translation / cytosolic large ribosomal subunit / tRNA binding / molecular adaptor activity / negative regulation of translation / rRNA binding / ribosome / structural constituent of ribosome / translation / response to antibiotic / negative regulation of DNA-templated transcription / mRNA binding / DNA binding / RNA binding / zinc ion binding / membrane / cytosol / cytoplasm 類似検索 - 分子機能 | |||||||||
生物種 | Escherichia coli BW25113 (大腸菌) / Paenibacillus larvae (バクテリア) / synthetic construct (人工物) | |||||||||
手法 | 単粒子再構成法 / クライオ電子顕微鏡法 / 解像度: 2.2 Å | |||||||||
データ登録者 | Koller TO / Wilson DN | |||||||||
資金援助 | ドイツ, 1件
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引用 | ジャーナル: bioRxiv / 年: 2024 タイトル: Paenilamicins from the honey bee pathogen are context-specific translocation inhibitors of protein synthesis. 著者: Timm O Koller / Max J Berger / Martino Morici / Helge Paternoga / Timur Bulatov / Adriana Di Stasi / Tam Dang / Andi Mainz / Karoline Raulf / Caillan Crowe-McAuliffe / Marco Scocchi / Mario ...著者: Timm O Koller / Max J Berger / Martino Morici / Helge Paternoga / Timur Bulatov / Adriana Di Stasi / Tam Dang / Andi Mainz / Karoline Raulf / Caillan Crowe-McAuliffe / Marco Scocchi / Mario Mardirossian / Bertrand Beckert / Nora Vázquez-Laslop / Alexander Mankin / Roderich D Süssmuth / Daniel N Wilson / 要旨: The paenilamicins are a group of hybrid non-ribosomal peptide-polyketide compounds produced by the honey bee pathogen that display activity against Gram-positive pathogens, such as . While ...The paenilamicins are a group of hybrid non-ribosomal peptide-polyketide compounds produced by the honey bee pathogen that display activity against Gram-positive pathogens, such as . While paenilamicins have been shown to inhibit protein synthesis, their mechanism of action has remained unclear. Here, we have determined structures of the paenilamicin PamB2 stalled ribosomes, revealing a unique binding site on the small 30S subunit located between the A- and P-site tRNAs. In addition to providing a precise description of interactions of PamB2 with the ribosome, the structures also rationalize the resistance mechanisms utilized by . We could further demonstrate that PamB2 interferes with the translocation of mRNA and tRNAs through the ribosome during translation elongation, and that this inhibitory activity is influenced by the presence of modifications at position 37 of the A-site tRNA. Collectively, our study defines the paenilamicins as a new class of context-specific translocation inhibitors. | |||||||||
履歴 |
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-構造の表示
添付画像 |
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-ダウンロードとリンク
-EMDBアーカイブ
マップデータ | emd_18950.map.gz | 78.3 MB | EMDBマップデータ形式 | |
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ヘッダ (付随情報) | emd-18950-v30.xml emd-18950.xml | 85.7 KB 85.7 KB | 表示 表示 | EMDBヘッダ |
FSC (解像度算出) | emd_18950_fsc.xml | 21 KB | 表示 | FSCデータファイル |
画像 | emd_18950.png | 96.4 KB | ||
Filedesc metadata | emd-18950.cif.gz | 15.2 KB | ||
その他 | emd_18950_additional_1.map.gz emd_18950_additional_2.map.gz emd_18950_half_map_1.map.gz emd_18950_half_map_2.map.gz | 671.2 MB 771.7 MB 671.4 MB 671.4 MB | ||
アーカイブディレクトリ | http://ftp.pdbj.org/pub/emdb/structures/EMD-18950 ftp://ftp.pdbj.org/pub/emdb/structures/EMD-18950 | HTTPS FTP |
-検証レポート
文書・要旨 | emd_18950_validation.pdf.gz | 904.7 KB | 表示 | EMDB検証レポート |
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文書・詳細版 | emd_18950_full_validation.pdf.gz | 904.3 KB | 表示 | |
XML形式データ | emd_18950_validation.xml.gz | 28 KB | 表示 | |
CIF形式データ | emd_18950_validation.cif.gz | 37.6 KB | 表示 | |
アーカイブディレクトリ | https://ftp.pdbj.org/pub/emdb/validation_reports/EMD-18950 ftp://ftp.pdbj.org/pub/emdb/validation_reports/EMD-18950 | HTTPS FTP |
-関連構造データ
-リンク
EMDBのページ | EMDB (EBI/PDBe) / EMDataResource |
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「今月の分子」の関連する項目 |
-マップ
ファイル | ダウンロード / ファイル: emd_18950.map.gz / 形式: CCP4 / 大きさ: 824 MB / タイプ: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) | ||||||||||||||||||||||||||||||||||||
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注釈 | Post processed masked map | ||||||||||||||||||||||||||||||||||||
投影像・断面図 | 画像のコントロール
画像は Spider により作成 | ||||||||||||||||||||||||||||||||||||
ボクセルのサイズ | X=Y=Z: 0.8 Å | ||||||||||||||||||||||||||||||||||||
密度 |
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対称性 | 空間群: 1 | ||||||||||||||||||||||||||||||||||||
詳細 | EMDB XML:
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-添付データ
-追加マップ: 3D refined map
ファイル | emd_18950_additional_1.map | ||||||||||||
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注釈 | 3D refined map | ||||||||||||
投影像・断面図 |
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密度ヒストグラム |
-追加マップ: Post processed map
ファイル | emd_18950_additional_2.map | ||||||||||||
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注釈 | Post processed map | ||||||||||||
投影像・断面図 |
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密度ヒストグラム |
-ハーフマップ: Half map 2
ファイル | emd_18950_half_map_1.map | ||||||||||||
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注釈 | Half map 2 | ||||||||||||
投影像・断面図 |
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密度ヒストグラム |
-ハーフマップ: Half map 1
ファイル | emd_18950_half_map_2.map | ||||||||||||
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注釈 | Half map 1 | ||||||||||||
投影像・断面図 |
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密度ヒストグラム |
-試料の構成要素
+全体 : Paenilamicin B2 bound to the 70S Escherichia coli ribosome
+超分子 #1: Paenilamicin B2 bound to the 70S Escherichia coli ribosome
+超分子 #2: 70S Escherichia coli ribosome
+超分子 #3: Paenilamicin B2
+分子 #1: Large ribosomal subunit protein bL33
+分子 #2: Large ribosomal subunit protein bL34
+分子 #3: Large ribosomal subunit protein bL35
+分子 #4: Large ribosomal subunit protein bL36A
+分子 #5: Large ribosomal subunit protein bL31A
+分子 #8: 30S ribosomal protein S2
+分子 #9: Small ribosomal subunit protein uS3
+分子 #10: Small ribosomal subunit protein uS4
+分子 #11: Small ribosomal subunit protein uS5
+分子 #12: 30S ribosomal protein S6, fully modified isoform
+分子 #13: 30S ribosomal protein S7
+分子 #14: Small ribosomal subunit protein uS8
+分子 #15: Small ribosomal subunit protein uS9
+分子 #16: Small ribosomal subunit protein uS10
+分子 #17: Small ribosomal subunit protein uS11
+分子 #18: Small ribosomal subunit protein uS12
+分子 #19: Small ribosomal subunit protein uS13
+分子 #20: Small ribosomal subunit protein uS14
+分子 #21: Small ribosomal subunit protein uS15
+分子 #22: 30S ribosomal protein S16
+分子 #23: Small ribosomal subunit protein uS17
+分子 #24: Small ribosomal subunit protein bS18
+分子 #25: Small ribosomal subunit protein uS19
+分子 #26: 30S ribosomal protein S20
+分子 #27: Small ribosomal subunit protein bS21
+分子 #33: Large ribosomal subunit protein uL2
+分子 #34: Large ribosomal subunit protein uL3
+分子 #35: Large ribosomal subunit protein uL4
+分子 #36: Large ribosomal subunit protein uL5
+分子 #37: Large ribosomal subunit protein uL6
+分子 #38: Large ribosomal subunit protein bL9
+分子 #39: Large ribosomal subunit protein uL13
+分子 #40: Large ribosomal subunit protein uL14
+分子 #41: 50S ribosomal protein L15
+分子 #42: Large ribosomal subunit protein uL16
+分子 #43: Large ribosomal subunit protein bL17
+分子 #44: Large ribosomal subunit protein uL18
+分子 #45: Large ribosomal subunit protein bL19
+分子 #46: Large ribosomal subunit protein bL20
+分子 #47: Large ribosomal subunit protein bL21
+分子 #48: Large ribosomal subunit protein uL22
+分子 #49: Large ribosomal subunit protein uL23
+分子 #50: Large ribosomal subunit protein uL24
+分子 #51: 50S ribosomal protein L25
+分子 #52: Large ribosomal subunit protein bL27
+分子 #53: Large ribosomal subunit protein bL28
+分子 #54: Large ribosomal subunit protein uL29
+分子 #55: Large ribosomal subunit protein uL30
+分子 #56: Large ribosomal subunit protein bL32
+分子 #57: Paenilamicin B2
+分子 #6: N-Formylmethionine tRNA
+分子 #7: 16S ribosomal RNA
+分子 #28: mRNA
+分子 #29: Isoeucine-tRNA
+分子 #30: Leucine-tRNA
+分子 #31: 23S ribosomal RNA
+分子 #32: 5S ribosomal RNA
+分子 #58: ZINC ION
+分子 #59: MAGNESIUM ION
+分子 #60: POTASSIUM ION
+分子 #61: water
-実験情報
-構造解析
手法 | クライオ電子顕微鏡法 |
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解析 | 単粒子再構成法 |
試料の集合状態 | particle |
-試料調製
緩衝液 | pH: 7.4 |
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凍結 | 凍結剤: ETHANE-PROPANE |
詳細 | 8 OD/mL of Ribosome |
-電子顕微鏡法
顕微鏡 | FEI TITAN KRIOS |
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撮影 | フィルム・検出器のモデル: FEI FALCON IV (4k x 4k) 平均電子線量: 60.0 e/Å2 |
電子線 | 加速電圧: 300 kV / 電子線源: FIELD EMISSION GUN |
電子光学系 | 照射モード: FLOOD BEAM / 撮影モード: BRIGHT FIELD / 最大 デフォーカス(公称値): 0.9 µm / 最小 デフォーカス(公称値): 0.4 µm |
実験機器 | モデル: Titan Krios / 画像提供: FEI Company |