- EMDB-17784: Structure of the human nuclear cap-binding complex bound to PHAX ... -
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基本情報
登録情報
データベース: EMDB / ID: EMD-17784
タイトル
Structure of the human nuclear cap-binding complex bound to PHAX and m7G-capped RNA
マップデータ
試料
複合体: Structure of the human nuclear cap-binding complex bound to PHAX and m7G-capped RNA
タンパク質・ペプチド: Nuclear cap-binding protein subunit 1
タンパク質・ペプチド: Nuclear cap-binding protein subunit 2
タンパク質・ペプチド: Phosphorylated adapter RNA export protein
リガンド: 7N-METHYL-8-HYDROGUANOSINE-5'-TRIPHOSPHATE
キーワード
Nuclear cap-binding complex / PHAX / Pol II transcript metabolism / RNA BINDING PROTEIN
機能・相同性
機能・相同性情報
mRNA cap binding complex binding / snRNA export from nucleus / nuclear cap binding complex / mRNA metabolic process / RNA cap binding complex / histone mRNA metabolic process / RNA stabilization / positive regulation of mRNA 3'-end processing / positive regulation of RNA export from nucleus / cap-dependent translational initiation ...mRNA cap binding complex binding / snRNA export from nucleus / nuclear cap binding complex / mRNA metabolic process / RNA cap binding complex / histone mRNA metabolic process / RNA stabilization / positive regulation of mRNA 3'-end processing / positive regulation of RNA export from nucleus / cap-dependent translational initiation / Processing of Intronless Pre-mRNAs / snRNA binding / positive regulation of RNA binding / RNA cap binding / alternative mRNA splicing, via spliceosome / miRNA-mediated post-transcriptional gene silencing / SLBP independent Processing of Histone Pre-mRNAs / primary miRNA processing / regulation of mRNA processing / SLBP Dependent Processing of Replication-Dependent Histone Pre-mRNAs / regulatory ncRNA-mediated post-transcriptional gene silencing / mRNA 3'-end processing / Transport of the SLBP independent Mature mRNA / Transport of the SLBP Dependant Mature mRNA / RNA 7-methylguanosine cap binding / mRNA 3'-end processing / Transport of Mature mRNA Derived from an Intronless Transcript / mRNA cis splicing, via spliceosome / RNA catabolic process / Transport of Mature mRNA derived from an Intron-Containing Transcript / Abortive elongation of HIV-1 transcript in the absence of Tat / regulation of translational initiation / nuclear-transcribed mRNA catabolic process, nonsense-mediated decay / positive regulation of mRNA splicing, via spliceosome / RNA Polymerase II Transcription Termination / FGFR2 alternative splicing / Signaling by FGFR2 IIIa TM / Formation of the Early Elongation Complex / Formation of the HIV-1 Early Elongation Complex / mRNA Capping / spliceosomal complex assembly / mRNA Splicing - Minor Pathway / Processing of Capped Intron-Containing Pre-mRNA / RNA polymerase II transcribes snRNA genes / Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC) / 7-methylguanosine mRNA capping / toxic substance binding / Formation of HIV-1 elongation complex containing HIV-1 Tat / Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC) / Formation of HIV elongation complex in the absence of HIV Tat / mRNA export from nucleus / Formation of RNA Pol II elongation complex / Cajal body / RNA Polymerase II Pre-transcription Events / mRNA Splicing - Major Pathway / RNA splicing / positive regulation of transcription elongation by RNA polymerase II / mRNA transcription by RNA polymerase II / Regulation of expression of SLITs and ROBOs / mRNA splicing, via spliceosome / protein transport / snRNP Assembly / positive regulation of cell growth / defense response to virus / molecular adaptor activity / ribonucleoprotein complex / mRNA binding / neuronal cell body / mitochondrion / DNA binding / RNA binding / nucleoplasm / nucleus / cytoplasm / cytosol 類似検索 - 分子機能
ジャーナル: Cell Rep / 年: 2024 タイトル: Structural basis for competitive binding of productive and degradative co-transcriptional effectors to the nuclear cap-binding complex. 著者: Etienne Dubiez / Erika Pellegrini / Maja Finderup Brask / William Garland / Anne-Emmanuelle Foucher / Karine Huard / Torben Heick Jensen / Stephen Cusack / Jan Kadlec / 要旨: The nuclear cap-binding complex (CBC) coordinates co-transcriptional maturation, transport, or degradation of nascent RNA polymerase II (Pol II) transcripts. CBC with its partner ARS2 forms mutually ...The nuclear cap-binding complex (CBC) coordinates co-transcriptional maturation, transport, or degradation of nascent RNA polymerase II (Pol II) transcripts. CBC with its partner ARS2 forms mutually exclusive complexes with diverse "effectors" that promote either productive or destructive outcomes. Combining AlphaFold predictions with structural and biochemical validation, we show how effectors NCBP3, NELF-E, ARS2, PHAX, and ZC3H18 form competing binary complexes with CBC and how PHAX, NCBP3, ZC3H18, and other effectors compete for binding to ARS2. In ternary CBC-ARS2 complexes with PHAX, NCBP3, or ZC3H18, ARS2 is responsible for the initial effector recruitment but inhibits their direct binding to the CBC. We show that in vivo ZC3H18 binding to both CBC and ARS2 is required for nuclear RNA degradation. We propose that recruitment of PHAX to CBC-ARS2 can lead, with appropriate cues, to competitive displacement of ARS2 and ZC3H18 from the CBC, thus promoting a productive rather than a degradative RNA fate.
全体 : Structure of the human nuclear cap-binding complex bound to PHAX ...
全体
名称: Structure of the human nuclear cap-binding complex bound to PHAX and m7G-capped RNA
要素
複合体: Structure of the human nuclear cap-binding complex bound to PHAX and m7G-capped RNA
タンパク質・ペプチド: Nuclear cap-binding protein subunit 1
タンパク質・ペプチド: Nuclear cap-binding protein subunit 2
タンパク質・ペプチド: Phosphorylated adapter RNA export protein
リガンド: 7N-METHYL-8-HYDROGUANOSINE-5'-TRIPHOSPHATE
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超分子 #1: Structure of the human nuclear cap-binding complex bound to PHAX ...
超分子
名称: Structure of the human nuclear cap-binding complex bound to PHAX and m7G-capped RNA タイプ: complex / ID: 1 / 親要素: 0 / 含まれる分子: #1-#3
由来(天然)
生物種: Homo sapiens (ヒト)
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分子 #1: Nuclear cap-binding protein subunit 1
分子
名称: Nuclear cap-binding protein subunit 1 / タイプ: protein_or_peptide / ID: 1 詳細: CBP80 construct has the N-terminal 20 residues (containing the NLS) deleted and replaced by a methionine コピー数: 1 / 光学異性体: LEVO