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Open data
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Basic information
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Title | Small subunit of yeast mitochondrial ribosome. | |||||||||
![]() | Oversampled composite map of local-mask refined maps with sharpening and local resolution filtering | |||||||||
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![]() | mitochondria / RIBOSOME | |||||||||
Function / homology | ![]() Branched-chain amino acid catabolism / 3-hydroxyisobutyryl-CoA hydrolase / 3-hydroxyisobutyryl-CoA hydrolase activity / mitochondrial translational initiation / valine catabolic process / Mitochondrial protein degradation / mitochondrial ribosome assembly / mitochondrial small ribosomal subunit / mitochondrial ribosome / sporulation resulting in formation of a cellular spore ...Branched-chain amino acid catabolism / 3-hydroxyisobutyryl-CoA hydrolase / 3-hydroxyisobutyryl-CoA hydrolase activity / mitochondrial translational initiation / valine catabolic process / Mitochondrial protein degradation / mitochondrial ribosome assembly / mitochondrial small ribosomal subunit / mitochondrial ribosome / sporulation resulting in formation of a cellular spore / mitochondrial translation / superoxide dismutase activity / mRNA processing / peroxisome / ribosomal small subunit biogenesis / ribosomal small subunit assembly / small ribosomal subunit / small ribosomal subunit rRNA binding / rRNA binding / mitochondrial inner membrane / ribosome / structural constituent of ribosome / hydrolase activity / translation / mRNA binding / GTP binding / mitochondrion / RNA binding / ATP binding / metal ion binding / cytoplasm Similarity search - Function | |||||||||
Biological species | ![]() ![]() | |||||||||
Method | single particle reconstruction / cryo EM / Resolution: 2.32 Å | |||||||||
![]() | Itoh Y / Chicherin I / Kamenski P / Amunts A | |||||||||
Funding support | European Union, ![]()
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![]() | ![]() Title: METTL17 is an Fe-S cluster checkpoint for mitochondrial translation. Authors: Tslil Ast / Yuzuru Itoh / Shayan Sadre / Jason G McCoy / Gil Namkoong / Jordan C Wengrod / Ivan Chicherin / Pallavi R Joshi / Piotr Kamenski / Daniel L M Suess / Alexey Amunts / Vamsi K Mootha / ![]() ![]() ![]() Abstract: Friedreich's ataxia (FA) is a debilitating, multisystemic disease caused by the depletion of frataxin (FXN), a mitochondrial iron-sulfur (Fe-S) cluster biogenesis factor. To understand the cellular ...Friedreich's ataxia (FA) is a debilitating, multisystemic disease caused by the depletion of frataxin (FXN), a mitochondrial iron-sulfur (Fe-S) cluster biogenesis factor. To understand the cellular pathogenesis of FA, we performed quantitative proteomics in FXN-deficient human cells. Nearly every annotated Fe-S cluster-containing protein was depleted, indicating that as a rule, cluster binding confers stability to Fe-S proteins. We also observed depletion of a small mitoribosomal assembly factor METTL17 and evidence of impaired mitochondrial translation. Using comparative sequence analysis, mutagenesis, biochemistry, and cryoelectron microscopy, we show that METTL17 binds to the mitoribosomal small subunit during late assembly and harbors a previously unrecognized [FeS] cluster required for its stability. METTL17 overexpression rescued the mitochondrial translation and bioenergetic defects, but not the cellular growth, of FXN-depleted cells. These findings suggest that METTL17 acts as an Fe-S cluster checkpoint, promoting translation of Fe-S cluster-rich oxidative phosphorylation (OXPHOS) proteins only when Fe-S cofactors are replete. | |||||||||
History |
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Structure visualization
Supplemental images |
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Downloads & links
-EMDB archive
Map data | ![]() | 1.3 GB | ![]() | |
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Header (meta data) | ![]() ![]() | 58.1 KB 58.1 KB | Display Display | ![]() |
Images | ![]() | 134.8 KB | ||
Filedesc metadata | ![]() | 13.8 KB | ||
Others | ![]() | 382.1 MB | ||
Archive directory | ![]() ![]() | HTTPS FTP |
-Validation report
Summary document | ![]() | 438.9 KB | Display | ![]() |
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Full document | ![]() | 438.5 KB | Display | |
Data in XML | ![]() | 9.2 KB | Display | |
Data in CIF | ![]() | 10.7 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 8om4MC ![]() 8om2C ![]() 8om3C C: citing same article ( M: atomic model generated by this map |
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Similar structure data | Similarity search - Function & homology ![]() |
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Links
EMDB pages | ![]() ![]() |
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Related items in Molecule of the Month |
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Map
File | ![]() | ||||||||||||||||||||
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Annotation | Oversampled composite map of local-mask refined maps with sharpening and local resolution filtering | ||||||||||||||||||||
Voxel size | X=Y=Z: 0.55333 Å | ||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||
Details | EMDB XML:
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-Supplemental data
-Additional map: Composite map of local-mask refined maps without sharpening
File | emd_16968_additional_1.map | ||||||||||||
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Annotation | Composite map of local-mask refined maps without sharpening | ||||||||||||
Projections & Slices |
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Density Histograms |
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Sample components
+Entire : Small subunit of mitochondrial ribosome
+Supramolecule #1: Small subunit of mitochondrial ribosome
+Macromolecule #1: 37S ribosomal protein MRP51, mitochondrial
+Macromolecule #2: 37S ribosomal protein MRP4, mitochondrial
+Macromolecule #3: Ribosomal protein VAR1, mitochondrial
+Macromolecule #4: 37S ribosomal protein NAM9, mitochondrial
+Macromolecule #5: 37S ribosomal protein S5, mitochondrial
+Macromolecule #6: 37S ribosomal protein MRP17, mitochondrial
+Macromolecule #7: 37S ribosomal protein S7, mitochondrial
+Macromolecule #8: 37S ribosomal protein S8, mitochondrial
+Macromolecule #9: 37S ribosomal protein S9, mitochondrial
+Macromolecule #10: 37S ribosomal protein S10, mitochondrial
+Macromolecule #11: 37S ribosomal protein S18, mitochondrial
+Macromolecule #12: 37S ribosomal protein S12, mitochondrial
+Macromolecule #13: 37S ribosomal protein SWS2, mitochondrial
+Macromolecule #14: 37S ribosomal protein MRP2, mitochondrial
+Macromolecule #15: 37S ribosomal protein S28, mitochondrial
+Macromolecule #16: 37S ribosomal protein S16, mitochondrial
+Macromolecule #17: 37S ribosomal protein S17, mitochondrial
+Macromolecule #18: 37S ribosomal protein RSM18, mitochondrial
+Macromolecule #19: 37S ribosomal protein S19, mitochondrial
+Macromolecule #20: 37S ribosomal protein MRP21, mitochondrial
+Macromolecule #21: 37S ribosomal protein S25, mitochondrial
+Macromolecule #22: 37S ribosomal protein PET123, mitochondrial
+Macromolecule #23: 37S ribosomal protein S23, mitochondrial
+Macromolecule #24: Mitochondrial 37S ribosomal protein S27
+Macromolecule #25: 37S ribosomal protein S24, mitochondrial
+Macromolecule #26: 37S ribosomal protein MRP10, mitochondrial
+Macromolecule #27: Mitochondrial mRNA-processing protein COX24
+Macromolecule #28: Protein FYV4, mitochondrial
+Macromolecule #29: 37S ribosomal protein S26, mitochondrial
+Macromolecule #30: 37S ribosomal protein MRP1, mitochondrial
+Macromolecule #31: 37S ribosomal protein MRP13, mitochondrial
+Macromolecule #32: 37S ribosomal protein S35, mitochondrial
+Macromolecule #33: 3-hydroxyisobutyryl-CoA hydrolase, mitochondrial
+Macromolecule #34: 15S mitochondrial rRNA
+Macromolecule #35: MAGNESIUM ION
+Macromolecule #36: POTASSIUM ION
+Macromolecule #37: DODECYL-BETA-D-MALTOSIDE
+Macromolecule #38: ADENOSINE-5'-TRIPHOSPHATE
+Macromolecule #39: water
-Experimental details
-Structure determination
Method | cryo EM |
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![]() | single particle reconstruction |
Aggregation state | particle |
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Sample preparation
Buffer | pH: 7.5 Component:
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Grid | Model: Quantifoil R2/1 / Material: COPPER / Support film - Material: CARBON / Support film - topology: CONTINUOUS / Support film - Film thickness: 3 / Pretreatment - Type: GLOW DISCHARGE / Pretreatment - Time: 30 sec. | ||||||||||||||||||
Vitrification | Cryogen name: ETHANE / Chamber humidity: 100 % / Chamber temperature: 277 K / Instrument: FEI VITROBOT MARK IV |
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Electron microscopy
Microscope | FEI TITAN KRIOS |
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Specialist optics | Energy filter - Name: GIF Quantum LS / Energy filter - Slit width: 40 eV |
Image recording | Film or detector model: GATAN K3 (6k x 4k) / Average electron dose: 32.0 e/Å2 |
Electron beam | Acceleration voltage: 300 kV / Electron source: ![]() |
Electron optics | C2 aperture diameter: 70.0 µm / Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Cs: 2.7 mm / Nominal defocus max: 2.8000000000000003 µm / Nominal defocus min: 0.5 µm / Nominal magnification: 165000 |
Sample stage | Specimen holder model: FEI TITAN KRIOS AUTOGRID HOLDER / Cooling holder cryogen: NITROGEN |
Experimental equipment | ![]() Model: Titan Krios / Image courtesy: FEI Company |