ジャーナル: Biophys Chem / 年: 2023 タイトル: Inside and outside of virus-like particles HBc and HBc/4M2e: A comprehensive study of the structure. 著者: V V Egorov / A V Shvetsov / E B Pichkur / A A Shaldzhyan / Ya A Zabrodskaya / D S Vinogradova / P A Nekrasov / A N Gorshkov / Yu P Garmay / A A Kovaleva / L A Stepanova / L M Tsybalova / T A ...著者: V V Egorov / A V Shvetsov / E B Pichkur / A A Shaldzhyan / Ya A Zabrodskaya / D S Vinogradova / P A Nekrasov / A N Gorshkov / Yu P Garmay / A A Kovaleva / L A Stepanova / L M Tsybalova / T A Shtam / A G Myasnikov / A L Konevega / 要旨: Hepatitis B virus core antigen (HBc) with the insertion of four external domains of the influenza A M2 protein (HBc/4M2e) form virus-like particles whose structure was studied using a combination of ...Hepatitis B virus core antigen (HBc) with the insertion of four external domains of the influenza A M2 protein (HBc/4M2e) form virus-like particles whose structure was studied using a combination of molecular modeling and cryo-electron microscopy (cryo-EM). It was also shown that self-assembling of the particles occurs inside bacterial cells, but despite the big inner volume of the core shell particle, purified HBc/4M2e contain an insignificant amount of bacterial proteins. It was shown that a fragment of the M2e corresponding to 4M2e insertion is prone to formation of amyloid-like fibrils. However, as the part of the immunodominant loop, M2e insertion does not show a tendency to intermolecular interaction. A full-atomic HBc-4M2e model with the resolution of about 3 Å (3.13 Å for particles of Т = 4 symmetry, 3.7 Å for particles of Т = 3 symmetry) was obtained by molecular modeling methods based on cryo-EM data.
全体 : Hepatitis B virus core antigen (HBc) with the insertion of four e...
全体
名称: Hepatitis B virus core antigen (HBc) with the insertion of four external domains of the influenza A M2 protein (T=3)
要素
複合体: Hepatitis B virus core antigen (HBc) with the insertion of four external domains of the influenza A M2 protein (T=3)
タンパク質・ペプチド: Core protein,Matrix protein 2,External core antigen
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超分子 #1: Hepatitis B virus core antigen (HBc) with the insertion of four e...
超分子
名称: Hepatitis B virus core antigen (HBc) with the insertion of four external domains of the influenza A M2 protein (T=3) タイプ: complex / ID: 1 / キメラ: Yes / 親要素: 0 / 含まれる分子: all
由来(天然)
生物種: Hepatitis B virus (B 型肝炎ウイルス)
分子量
理論値: 30.84 kDa/nm
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分子 #1: Core protein,Matrix protein 2,External core antigen