Journal: Biophys Chem / Year: 2023 Title: Inside and outside of virus-like particles HBc and HBc/4M2e: A comprehensive study of the structure. Authors: V V Egorov / A V Shvetsov / E B Pichkur / A A Shaldzhyan / Ya A Zabrodskaya / D S Vinogradova / P A Nekrasov / A N Gorshkov / Yu P Garmay / A A Kovaleva / L A Stepanova / L M Tsybalova / T A ...Authors: V V Egorov / A V Shvetsov / E B Pichkur / A A Shaldzhyan / Ya A Zabrodskaya / D S Vinogradova / P A Nekrasov / A N Gorshkov / Yu P Garmay / A A Kovaleva / L A Stepanova / L M Tsybalova / T A Shtam / A G Myasnikov / A L Konevega / Abstract: Hepatitis B virus core antigen (HBc) with the insertion of four external domains of the influenza A M2 protein (HBc/4M2e) form virus-like particles whose structure was studied using a combination of ...Hepatitis B virus core antigen (HBc) with the insertion of four external domains of the influenza A M2 protein (HBc/4M2e) form virus-like particles whose structure was studied using a combination of molecular modeling and cryo-electron microscopy (cryo-EM). It was also shown that self-assembling of the particles occurs inside bacterial cells, but despite the big inner volume of the core shell particle, purified HBc/4M2e contain an insignificant amount of bacterial proteins. It was shown that a fragment of the M2e corresponding to 4M2e insertion is prone to formation of amyloid-like fibrils. However, as the part of the immunodominant loop, M2e insertion does not show a tendency to intermolecular interaction. A full-atomic HBc-4M2e model with the resolution of about 3 Å (3.13 Å for particles of Т = 4 symmetry, 3.7 Å for particles of Т = 3 symmetry) was obtained by molecular modeling methods based on cryo-EM data.
Entire : Hepatitis B virus core antigen (HBc) with the insertion of four e...
Entire
Name: Hepatitis B virus core antigen (HBc) with the insertion of four external domains of the influenza A M2 protein (T=3)
Components
Complex: Hepatitis B virus core antigen (HBc) with the insertion of four external domains of the influenza A M2 protein (T=3)
Protein or peptide: Core protein,Matrix protein 2,External core antigen
-
Supramolecule #1: Hepatitis B virus core antigen (HBc) with the insertion of four e...
Supramolecule
Name: Hepatitis B virus core antigen (HBc) with the insertion of four external domains of the influenza A M2 protein (T=3) type: complex / ID: 1 / Chimera: Yes / Parent: 0 / Macromolecule list: all
Source (natural)
Organism: Hepatitis B virus
Molecular weight
Theoretical: 30.84 kDa/nm
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Macromolecule #1: Core protein,Matrix protein 2,External core antigen
Macromolecule
Name: Core protein,Matrix protein 2,External core antigen / type: protein_or_peptide / ID: 1 / Number of copies: 3 / Enantiomer: LEVO
Source (natural)
Organism: Hepatitis B virus / Strain: isolate France/Tiollais/1979
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