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基本情報
登録情報 | ![]() | ||||||||||||
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タイトル | Membrane-bound CHMP2A-CHMP3 filament (410 Angstrom diameter) | ||||||||||||
![]() | Membrane-bound CHMP2A-CHMP3 filament (410 Angstrom diameter) | ||||||||||||
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![]() | Cryo Electron Microscopy / Helical Reconstruction / Membrane-bound CHMP2A-CHMP3 filament / Negative-curvature membrane / CYTOSOLIC PROTEIN | ||||||||||||
機能・相同性 | ![]() negative regulation of centriole elongation / regulation of endosome size / membrane invagination / amphisome membrane / multivesicular body-lysosome fusion / vesicle fusion with vacuole / suppression of viral release by host / ESCRT III complex disassembly / late endosome to lysosome transport / ESCRT III complex ...negative regulation of centriole elongation / regulation of endosome size / membrane invagination / amphisome membrane / multivesicular body-lysosome fusion / vesicle fusion with vacuole / suppression of viral release by host / ESCRT III complex disassembly / late endosome to lysosome transport / ESCRT III complex / kinetochore microtubule / endosome transport via multivesicular body sorting pathway / regulation of centrosome duplication / nuclear membrane reassembly / Sealing of the nuclear envelope (NE) by ESCRT-III / midbody abscission / multivesicular body sorting pathway / membrane coat / membrane fission / plasma membrane repair / late endosome to vacuole transport / phosphatidylcholine binding / multivesicular body membrane / positive regulation of exosomal secretion / ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway / multivesicular body assembly / regulation of mitotic spindle assembly / Translation of Replicase and Assembly of the Replication Transcription Complex / exit from mitosis / Lysosome Vesicle Biogenesis / regulation of early endosome to late endosome transport / mitotic metaphase chromosome alignment / Macroautophagy / molecular function inhibitor activity / ubiquitin-specific protease binding / nucleus organization / viral budding via host ESCRT complex / positive regulation of cytokinesis / autophagosome membrane / autophagosome maturation / viral release from host cell / protein polymerization / Pyroptosis / nuclear pore / Endosomal Sorting Complex Required For Transport (ESCRT) / phosphatidylinositol-4,5-bisphosphate binding / multivesicular body / viral budding from plasma membrane / HCMV Late Events / macroautophagy / Late endosomal microautophagy / establishment of protein localization / Budding and maturation of HIV virion / protein homooligomerization / kinetochore / autophagy / protein transport / late endosome / nuclear envelope / Translation of Replicase and Assembly of the Replication Transcription Complex / midbody / cytoplasmic vesicle / early endosome / protein domain specific binding / lysosomal membrane / chromatin / apoptotic process / extracellular exosome / identical protein binding / membrane / plasma membrane / cytosol 類似検索 - 分子機能 | ||||||||||||
生物種 | ![]() | ||||||||||||
手法 | らせん対称体再構成法 / クライオ電子顕微鏡法 / 解像度: 3.6 Å | ||||||||||||
![]() | Azad K / Desfosses A / Effantin G / Schoehn G / Weissenhorn W | ||||||||||||
資金援助 | ![]()
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![]() | ![]() タイトル: Structural basis of CHMP2A-CHMP3 ESCRT-III polymer assembly and membrane cleavage. 著者: Kimi Azad / Delphine Guilligay / Cecile Boscheron / Sourav Maity / Nicola De Franceschi / Guidenn Sulbaran / Gregory Effantin / Haiyan Wang / Jean-Philippe Kleman / Patricia Bassereau / Guy ...著者: Kimi Azad / Delphine Guilligay / Cecile Boscheron / Sourav Maity / Nicola De Franceschi / Guidenn Sulbaran / Gregory Effantin / Haiyan Wang / Jean-Philippe Kleman / Patricia Bassereau / Guy Schoehn / Wouter H Roos / Ambroise Desfosses / Winfried Weissenhorn / ![]() ![]() 要旨: The endosomal sorting complex required for transport (ESCRT) is a highly conserved protein machinery that drives a divers set of physiological and pathological membrane remodeling processes. However, ...The endosomal sorting complex required for transport (ESCRT) is a highly conserved protein machinery that drives a divers set of physiological and pathological membrane remodeling processes. However, the structural basis of ESCRT-III polymers stabilizing, constricting and cleaving negatively curved membranes is yet unknown. Here we present cryo-EM structures of membrane-coated CHMP2A-CHMP3 filaments from Homo sapiens of two different diameters at 3.3 and 3.6 Å resolution. The structures reveal helical filaments assembled by CHMP2A-CHMP3 heterodimers in the open ESCRT-III conformation, which generates a partially positive charged membrane interaction surface, positions short N-terminal motifs for membrane interaction and the C-terminal VPS4 target sequence toward the tube interior. Inter-filament interactions are electrostatic, which may facilitate filament sliding upon VPS4-mediated polymer remodeling. Fluorescence microscopy as well as high-speed atomic force microscopy imaging corroborate that VPS4 can constrict and cleave CHMP2A-CHMP3 membrane tubes. We therefore conclude that CHMP2A-CHMP3-VPS4 act as a minimal membrane fission machinery. | ||||||||||||
履歴 |
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構造の表示
添付画像 |
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ダウンロードとリンク
-EMDBアーカイブ
マップデータ | ![]() | 91 MB | ![]() | |
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ヘッダ (付随情報) | ![]() ![]() | 18.7 KB 18.7 KB | 表示 表示 | ![]() |
FSC (解像度算出) | ![]() | 27 KB | 表示 | ![]() |
画像 | ![]() | 163.5 KB | ||
Filedesc metadata | ![]() | 6.2 KB | ||
その他 | ![]() ![]() | 1.4 GB 1.4 GB | ||
アーカイブディレクトリ | ![]() ![]() | HTTPS FTP |
-関連構造データ
関連構造データ | ![]() 7zchMC ![]() 7zcgC M: このマップから作成された原子モデル C: 同じ文献を引用 ( |
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類似構造データ | 類似検索 - 機能・相同性 ![]() |
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リンク
EMDBのページ | ![]() ![]() |
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「今月の分子」の関連する項目 |
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マップ
ファイル | ![]() | ||||||||||||||||||||
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注釈 | Membrane-bound CHMP2A-CHMP3 filament (410 Angstrom diameter) | ||||||||||||||||||||
ボクセルのサイズ | X=Y=Z: 1.052 Å | ||||||||||||||||||||
密度 |
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対称性 | 空間群: 1 | ||||||||||||||||||||
詳細 | EMDB XML:
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-添付データ
-ハーフマップ: Membrane-bound CHMP2A-CHMP3 filament (410 Angstrom diameter) half map...
ファイル | emd_14631_half_map_1.map | ||||||||||||
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注釈 | Membrane-bound CHMP2A-CHMP3 filament (410 Angstrom diameter) half map 1 | ||||||||||||
投影像・断面図 |
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密度ヒストグラム |
-ハーフマップ: Membrane-bound CHMP2A-CHMP3 filament (410 Angstrom diameter) half map...
ファイル | emd_14631_half_map_2.map | ||||||||||||
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注釈 | Membrane-bound CHMP2A-CHMP3 filament (410 Angstrom diameter) half map 2 | ||||||||||||
投影像・断面図 |
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密度ヒストグラム |
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試料の構成要素
-全体 : Membrane-bound CHMP2A-CHMP3 filament (410 Angstrom Diameter) cryo...
全体 | 名称: Membrane-bound CHMP2A-CHMP3 filament (410 Angstrom Diameter) cryo-EM map |
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要素 |
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-超分子 #1: Membrane-bound CHMP2A-CHMP3 filament (410 Angstrom Diameter) cryo...
超分子 | 名称: Membrane-bound CHMP2A-CHMP3 filament (410 Angstrom Diameter) cryo-EM map タイプ: complex / ID: 1 / 親要素: 0 / 含まれる分子: all |
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由来(天然) | 生物種: ![]() |
-分子 #1: Charged multivesicular body protein 2a
分子 | 名称: Charged multivesicular body protein 2a / タイプ: protein_or_peptide / ID: 1 / コピー数: 1 / 光学異性体: LEVO |
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由来(天然) | 生物種: ![]() |
分子量 | 理論値: 17.305488 KDa |
組換発現 | 生物種: ![]() ![]() |
配列 | 文字列: RKTPEELLRQ NQRALNRAMR ELDRERQKLE TQEKKIIADI KKMAKQGQMD AVRIMAKDLV RTRRYVRKFV LMRANIQAVS LKIQTLKSN NSMAQAMKGV TKAMGTMNRQ LKLPQIQKIM MEFERQAEIM DMKEEMMNDA IDDAMGDE UniProtKB: Charged multivesicular body protein 2a |
-分子 #2: Charged multivesicular body protein 3
分子 | 名称: Charged multivesicular body protein 3 / タイプ: protein_or_peptide / ID: 2 / コピー数: 1 / 光学異性体: LEVO |
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由来(天然) | 生物種: ![]() |
分子量 | 理論値: 18.429684 KDa |
組換発現 | 生物種: ![]() ![]() |
配列 | 文字列: PPKELVNEWS LKIRKEMRVV DRQIRDIQRE EEKVKRSVKD AAKKGQKDVC IVLAKEMIRS RKAVSKLYAS KAHMNSVLMG MKNQLAVLR VAGSLQKSTE VMKAMQSLVK IPEIQATMRE LSKEMMKAGI IEEMLEDTFE SMDDQEEMEE EAEMEIDRIL UniProtKB: Charged multivesicular body protein 3 |
-実験情報
-構造解析
手法 | クライオ電子顕微鏡法 |
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![]() | らせん対称体再構成法 |
試料の集合状態 | filament |
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試料調製
濃度 | 1 mg/mL | ||||||||||||
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緩衝液 | pH: 7.4 構成要素:
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グリッド | モデル: Quantifoil R1.2/1.3 / 材質: COPPER / 支持フィルム - 材質: CARBON / 支持フィルム - トポロジー: HOLEY / 前処理 - タイプ: GLOW DISCHARGE | ||||||||||||
凍結 | 凍結剤: ETHANE / チャンバー内湿度: 100 % / チャンバー内温度: 293.15 K / 装置: FEI VITROBOT MARK IV |
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電子顕微鏡法
顕微鏡 | FEI TITAN KRIOS |
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特殊光学系 | エネルギーフィルター - 名称: GIF Bioquantum / エネルギーフィルター - スリット幅: 20 eV |
撮影 | フィルム・検出器のモデル: GATAN K2 SUMMIT (4k x 4k) 実像数: 5028 / 平均露光時間: 5.0 sec. / 平均電子線量: 24.0 e/Å2 |
電子線 | 加速電圧: 300 kV / 電子線源: ![]() |
電子光学系 | 照射モード: FLOOD BEAM / 撮影モード: BRIGHT FIELD / 最大 デフォーカス(公称値): 1.5 µm / 最小 デフォーカス(公称値): 0.5 µm |
試料ステージ | ホルダー冷却材: NITROGEN |
実験機器 | ![]() モデル: Titan Krios / 画像提供: FEI Company |