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データを開く
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基本情報
登録情報 | ![]() | |||||||||
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タイトル | EIF6-bound large subunit of the human ribosome | |||||||||
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![]() | Ribosome / EIF6 / human / SDS / TRANSLATION | |||||||||
機能・相同性 | ![]() lamin filament / regulation of fatty acid biosynthetic process / regulation of megakaryocyte differentiation / miRNA-mediated post-transcriptional gene silencing / miRNA-mediated gene silencing by inhibition of translation / exit from mitosis / eukaryotic 80S initiation complex / negative regulation of protein neddylation / optic nerve development / regulation of translation involved in cellular response to UV ...lamin filament / regulation of fatty acid biosynthetic process / regulation of megakaryocyte differentiation / miRNA-mediated post-transcriptional gene silencing / miRNA-mediated gene silencing by inhibition of translation / exit from mitosis / eukaryotic 80S initiation complex / negative regulation of protein neddylation / optic nerve development / regulation of translation involved in cellular response to UV / axial mesoderm development / negative regulation of formation of translation preinitiation complex / regulation of G1 to G0 transition / ribosomal protein import into nucleus / 90S preribosome assembly / protein-DNA complex disassembly / positive regulation of intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator / retinal ganglion cell axon guidance / GAIT complex / positive regulation of DNA damage response, signal transduction by p53 class mediator / TORC2 complex binding / G1 to G0 transition / regulation of glycolytic process / middle ear morphogenesis / regulation of reactive oxygen species metabolic process / cytoplasmic side of rough endoplasmic reticulum membrane / maturation of 5.8S rRNA / negative regulation of ubiquitin protein ligase activity / homeostatic process / macrophage chemotaxis / lung morphogenesis / male meiosis I / positive regulation of natural killer cell proliferation / ribosomal large subunit binding / Protein hydroxylation / Peptide chain elongation / Selenocysteine synthesis / Formation of a pool of free 40S subunits / Eukaryotic Translation Termination / ubiquitin ligase inhibitor activity / cellular response to actinomycin D / blastocyst development / Response of EIF2AK4 (GCN2) to amino acid deficiency / SRP-dependent cotranslational protein targeting to membrane / negative regulation of ubiquitin-dependent protein catabolic process / positive regulation of signal transduction by p53 class mediator / Viral mRNA Translation / Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC) / protein localization to nucleus / GTP hydrolysis and joining of the 60S ribosomal subunit / L13a-mediated translational silencing of Ceruloplasmin expression / positive regulation of protein binding / Major pathway of rRNA processing in the nucleolus and cytosol / ribosomal subunit export from nucleus / protein targeting / protein-RNA complex assembly / Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC) / maturation of LSU-rRNA / rough endoplasmic reticulum / MDM2/MDM4 family protein binding / negative regulation of proteasomal ubiquitin-dependent protein catabolic process / translation initiation factor activity / embryo implantation / cytosolic ribosome / ossification / assembly of large subunit precursor of preribosome / positive regulation of translation / maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / regulation of signal transduction by p53 class mediator / ribosomal large subunit biogenesis / cytosolic ribosome assembly / mRNA 3'-UTR binding / skeletal system development / DNA damage response, signal transduction by p53 class mediator / sensory perception of sound / cellular response to gamma radiation / response to insulin / bone development / mRNA 5'-UTR binding / cellular response to type II interferon / Regulation of expression of SLITs and ROBOs / transcription coactivator binding / cytoplasmic ribonucleoprotein granule / rRNA processing / antimicrobial humoral immune response mediated by antimicrobial peptide / cellular response to UV / large ribosomal subunit / heparin binding / regulation of translation / ribosome binding / retina development in camera-type eye / cell body / 5S rRNA binding / ribosomal large subunit assembly / large ribosomal subunit rRNA binding / spermatogenesis / response to lipopolysaccharide / defense response to Gram-negative bacterium / killing of cells of another organism / cytosolic large ribosomal subunit 類似検索 - 分子機能 | |||||||||
生物種 | ![]() | |||||||||
手法 | 単粒子再構成法 / クライオ電子顕微鏡法 / 解像度: 2.4 Å | |||||||||
![]() | Faille A / Warren AJ | |||||||||
資金援助 | ![]()
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![]() | ![]() タイトル: Structure of human EIF6-bound large ribosomal subunit 著者: Faille A / Warren AJ | |||||||||
履歴 |
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構造の表示
添付画像 |
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ダウンロードとリンク
-EMDBアーカイブ
マップデータ | ![]() | 403.2 MB | ![]() | |
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ヘッダ (付随情報) | ![]() ![]() | 57.1 KB 57.1 KB | 表示 表示 | ![]() |
FSC (解像度算出) | ![]() | 18 KB | 表示 | ![]() |
画像 | ![]() | 70 KB | ||
Filedesc metadata | ![]() | 13.7 KB | ||
アーカイブディレクトリ | ![]() ![]() | HTTPS FTP |
-検証レポート
文書・要旨 | ![]() | 549.3 KB | 表示 | ![]() |
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文書・詳細版 | ![]() | 548.8 KB | 表示 | |
XML形式データ | ![]() | 16.2 KB | 表示 | |
CIF形式データ | ![]() | 22.4 KB | 表示 | |
アーカイブディレクトリ | ![]() ![]() | HTTPS FTP |
-関連構造データ
関連構造データ | ![]() 7ow7MC M: このマップから作成された原子モデル C: 同じ文献を引用 ( |
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類似構造データ | 類似検索 - 機能・相同性 ![]() |
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リンク
EMDBのページ | ![]() ![]() |
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「今月の分子」の関連する項目 |
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マップ
ファイル | ![]() | ||||||||||||||||||||||||||||||||||||
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投影像・断面図 | 画像のコントロール
画像は Spider により作成 | ||||||||||||||||||||||||||||||||||||
ボクセルのサイズ | X=Y=Z: 0.83 Å | ||||||||||||||||||||||||||||||||||||
密度 |
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対称性 | 空間群: 1 | ||||||||||||||||||||||||||||||||||||
詳細 | EMDB XML:
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-添付データ
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試料の構成要素
+全体 : Human large ribosomal subunit
+超分子 #1: Human large ribosomal subunit
+分子 #1: 28S rRNA
+分子 #2: 5S rRNA
+分子 #3: 5.8S ribosomal RNA
+分子 #4: 60S ribosomal protein L8
+分子 #5: 60S ribosomal protein L3 isoform a
+分子 #6: 60S ribosomal protein L4
+分子 #7: 60S ribosomal protein L5
+分子 #8: 60S ribosomal protein L6
+分子 #9: 60S ribosomal protein L7
+分子 #10: 60S ribosomal protein L7a
+分子 #11: 60S ribosomal protein L9
+分子 #12: 60S ribosomal protein L10-like
+分子 #13: 60S ribosomal protein L11
+分子 #14: 60S ribosomal protein L13
+分子 #15: 60S ribosomal protein L14
+分子 #16: 60S ribosomal protein L15
+分子 #17: 60S ribosomal protein L13a
+分子 #18: 60S ribosomal protein L17
+分子 #19: 60S ribosomal protein L18
+分子 #20: 60S ribosomal protein L19
+分子 #21: 60S ribosomal protein L18a
+分子 #22: 60S ribosomal protein L21
+分子 #23: 60S ribosomal protein L23a
+分子 #24: 60S ribosomal protein L26
+分子 #25: 60S ribosomal protein L27
+分子 #26: 60S ribosomal protein L27a
+分子 #27: 60S ribosomal protein L29
+分子 #28: 60S ribosomal protein L30
+分子 #29: 60S ribosomal protein L31
+分子 #30: 60S ribosomal protein L32
+分子 #31: 60S ribosomal protein L35a
+分子 #32: 60S ribosomal protein L34
+分子 #33: 60S ribosomal protein L35
+分子 #34: 60S ribosomal protein L36
+分子 #35: 60S ribosomal protein L37
+分子 #36: 60S ribosomal protein L38
+分子 #37: 60S ribosomal protein L39
+分子 #38: 60S ribosomal protein L36a
+分子 #39: 60S ribosomal protein L37a
+分子 #40: 60S ribosomal protein L28
+分子 #41: Eukaryotic translation initiation factor 6
+分子 #42: 60S ribosomal protein L23
+分子 #43: 60S ribosomal protein L24
+分子 #44: MAGNESIUM ION
+分子 #45: ZINC ION
+分子 #46: POTASSIUM ION
+分子 #47: SODIUM ION
+分子 #48: BROMIDE ION
+分子 #49: water
-実験情報
-構造解析
手法 | クライオ電子顕微鏡法 |
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![]() | 単粒子再構成法 |
試料の集合状態 | particle |
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試料調製
緩衝液 | pH: 7.4 |
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凍結 | 凍結剤: ETHANE |
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電子顕微鏡法
顕微鏡 | FEI TITAN KRIOS |
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撮影 | フィルム・検出器のモデル: GATAN K3 BIOQUANTUM (6k x 4k) 平均電子線量: 40.0 e/Å2 |
電子線 | 加速電圧: 300 kV / 電子線源: ![]() |
電子光学系 | 照射モード: FLOOD BEAM / 撮影モード: BRIGHT FIELD |
実験機器 | ![]() モデル: Titan Krios / 画像提供: FEI Company |