- EMDB-12600: Structure of the Toxoplasma gondii kinase Ron13, kinase-dead mutant -
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基本情報
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データベース: EMDB / ID: EMD-12600
タイトル
Structure of the Toxoplasma gondii kinase Ron13, kinase-dead mutant
マップデータ
Ron13 cryo-EM map
試料
細胞器官・細胞要素: Secretory pathway kinase Ron13
タンパク質・ペプチド: Protein kinase domain-containing protein
機能・相同性
機能・相同性情報
membrane => GO:0016020 / protein kinase activity / ATP binding 類似検索 - 分子機能
Serine/threonine-protein kinase, active site / Serine/Threonine protein kinases active-site signature. / Serine/Threonine protein kinases, catalytic domain / Protein kinase, ATP binding site / Protein kinases ATP-binding region signature. / Protein kinase domain profile. / Protein kinase domain / Protein kinase-like domain superfamily 類似検索 - ドメイン・相同性
Protein kinase domain-containing protein 類似検索 - 構成要素
ジャーナル: Nat Commun / 年: 2021 タイトル: Structural insights into an atypical secretory pathway kinase crucial for Toxoplasma gondii invasion. 著者: Gaëlle Lentini / Rouaa Ben Chaabene / Oscar Vadas / Chandra Ramakrishnan / Budhaditya Mukherjee / Ved Mehta / Matteo Lunghi / Jonas Grossmann / Bohumil Maco / Rémy Visentin / Adrian B Hehl ...著者: Gaëlle Lentini / Rouaa Ben Chaabene / Oscar Vadas / Chandra Ramakrishnan / Budhaditya Mukherjee / Ved Mehta / Matteo Lunghi / Jonas Grossmann / Bohumil Maco / Rémy Visentin / Adrian B Hehl / Volodymyr M Korkhov / Dominique Soldati-Favre / 要旨: Active host cell invasion by the obligate intracellular apicomplexan parasites relies on the formation of a moving junction, which connects parasite and host cell plasma membranes during entry. ...Active host cell invasion by the obligate intracellular apicomplexan parasites relies on the formation of a moving junction, which connects parasite and host cell plasma membranes during entry. Invading Toxoplasma gondii tachyzoites secrete their rhoptry content and insert a complex of RON proteins on the cytoplasmic side of the host cell membrane providing an anchor to which the parasite tethers. Here we show that a rhoptry-resident kinase RON13 is a key virulence factor that plays a crucial role in host cell entry. Cryo-EM, kinase assays, phosphoproteomics and cellular analyses reveal that RON13 is a secretory pathway kinase of atypical structure that phosphorylates rhoptry proteins including the components of the RON complex. Ultimately, RON13 kinase activity controls host cell invasion by anchoring the moving junction at the parasite-host cell interface.