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- EMDB-74497: Rabbit Psoas Skeletal Muscle Thick Filaments -

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Basic information

Entry
Database: EMDB / ID: EMD-74497
TitleRabbit Psoas Skeletal Muscle Thick Filaments
Map data
Sample
  • Organelle or cellular component: Rabbit Psoas Skeletal Muscle Thick Filaments
    • Organelle or cellular component: Myosin II
      • Organelle or cellular component: Titin
        • Organelle or cellular component: Fast myosin-binding protein C
Keywordsmyosin II / skeletal muscle / Fast myosin-binding protein C / Titin / MOTOR PROTEIN
Biological speciesOryctolagus cuniculus (rabbit)
Methodsingle particle reconstruction / cryo EM / Resolution: 9.94 Å
AuthorsTaylor K / Gholami Tilko P
Funding support United States, 4 items
OrganizationGrant numberCountry
National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)R35 GM139616 United States
National Institutes of Health/National Heart, Lung, and Blood Institute (NIH/NHLBI)HL128683 United States
National Institutes of Health/National Heart, Lung, and Blood Institute (NIH/NHLBI)HL160966 United States
National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)R24 GM145964 United States
CitationJournal: To Be Published
Title: Rabbit Psoas Skeletal Muscle Thick Filaments C-zone
Authors: Taylor K / Gholami Tilko P
History
DepositionDec 15, 2025-
Header (metadata) releaseSep 2, 2026-
Map releaseSep 2, 2026-
UpdateSep 2, 2026-
Current statusSep 2, 2026Processing site: RCSB / Status: Released

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Structure visualization

Supplemental images

emd_74497.png

Downloads & links

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Map

FileDownload / File: emd_74497.map.gz / Format: CCP4 / Size: 244.1 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Projections & slices

Image control

Size
Brightness
Contrast
Others
AxesZ (Sec.)Y (Row.)X (Col.)
2.6 Å/pix.
x 400 pix.
= 1038.24 Å		2.6 Å/pix.
x 400 pix.
= 1038.24 Å		2.6 Å/pix.
x 400 pix.
= 1038.24 Å

Surface

Projections

Slices (1/3)

Slices (1/2)

Slices (2/3)

Images are generated by Spider.

Voxel sizeX=Y=Z: 2.5956 Å
Density

Histogram

Histogram (log scale)
Contour LevelBy AUTHOR: 0.123
Minimum - Maximum-0.0010698145 - 1.3933698
Average (Standard dev.)0.0042392733 (±0.042700343)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions400400400
Spacing400400400
CellA=B=C: 1038.24 Å
α=β=γ: 90.0 °

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Supplemental data

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Half map: #1

Fileemd_74497_half_map_1.map
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Half map: #2

Fileemd_74497_half_map_2.map
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Sample components

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Entire : Rabbit Psoas Skeletal Muscle Thick Filaments

EntireName: Rabbit Psoas Skeletal Muscle Thick Filaments
Components
  • Organelle or cellular component: Rabbit Psoas Skeletal Muscle Thick Filaments
    • Organelle or cellular component: Myosin II
      • Organelle or cellular component: Titin
        • Organelle or cellular component: Fast myosin-binding protein C

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Supramolecule #1: Rabbit Psoas Skeletal Muscle Thick Filaments

SupramoleculeName: Rabbit Psoas Skeletal Muscle Thick Filaments / type: organelle_or_cellular_component / ID: 1 / Parent: 0 / Macromolecule list: #1
Source (natural)Organism: Oryctolagus cuniculus (rabbit) / Tissue: skeletal muscle / Organelle: Myosin filament / Location in cell: Myofibril

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Supramolecule #2: Myosin II

SupramoleculeName: Myosin II / type: organelle_or_cellular_component / ID: 2 / Parent: 1
Source (natural)Organism: Oryctolagus cuniculus (rabbit) / Tissue: skeletal muscle / Organelle: Myosin filament / Location in cell: Myofibril

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Supramolecule #3: Titin

SupramoleculeName: Titin / type: organelle_or_cellular_component / ID: 3 / Parent: 2
Source (natural)Organism: Oryctolagus cuniculus (rabbit) / Tissue: skeletal muscle / Organelle: Myosin filament / Location in cell: Myofibril

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Supramolecule #4: Fast myosin-binding protein C

SupramoleculeName: Fast myosin-binding protein C / type: organelle_or_cellular_component / ID: 4 / Parent: 3
Source (natural)Organism: Oryctolagus cuniculus (rabbit) / Tissue: skeletal muscle / Organelle: Myosin filament / Location in cell: Myofibril

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation statefilament

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Sample preparation

BufferpH: 6.8
VitrificationCryogen name: ETHANE / Chamber humidity: 90 % / Chamber temperature: 293 K

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Electron microscopy

MicroscopeTFS KRIOS
Image recordingFilm or detector model: FEI FALCON IV (4k x 4k) / Average electron dose: 48.87 e/Å2
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Nominal defocus max: 3.0 µm / Nominal defocus min: 1.0 µm
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

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Image processing

CTF correctionType: PHASE FLIPPING ONLY
Startup modelType of model: NONE / Details: ab initio
Final reconstructionResolution.type: BY AUTHOR / Resolution: 9.94 Å / Resolution method: FSC 0.143 CUT-OFF / Software - Name: cryoSPARC / Number images used: 43696
Initial angle assignmentType: NOT APPLICABLE
Final angle assignmentType: NOT APPLICABLE
FSC plot (resolution estimation)

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