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- EMDB-72382: Human antibody Fab MPV499 bound to hMPV DsCav-ES2 -

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Basic information

Entry
Database: EMDB / ID: EMD-72382
TitleHuman antibody Fab MPV499 bound to hMPV DsCav-ES2
Map data
Sample
  • Complex: Human antibody Fab MPV499 bound to hMPV DsCav-ES2
    • Protein or peptide: MPV499 bound to hMPV DsCav-Es2 F protein
Keywordsantibody / human metapneumovirus / ANTIVIRAL PROTEIN
Biological speciesHomo sapiens (human)
Methodsingle particle reconstruction / cryo EM / Resolution: 6.57 Å
AuthorsEsfahani BG / Mousa J / Khalil AM
Funding support United States, 1 items
OrganizationGrant numberCountry
National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)R01 AI143865 United States
CitationJournal: To Be Published
Title: Structural Basis for Childhood Antibody Recognition of The Human Metapneumovirus Fusion Protein
Authors: Esfahani BG / Khalil AM / Mousa J
History
DepositionAug 28, 2025-
Header (metadata) releaseSep 10, 2025-
Map releaseSep 10, 2025-
UpdateSep 10, 2025-
Current statusSep 10, 2025Processing site: RCSB / Status: Released

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Structure visualization

Supplemental images

emd_72382.png

Downloads & links

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Map

FileDownload / File: emd_72382.map.gz / Format: CCP4 / Size: 149.9 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Projections & slices

Image control

Size
Brightness
Contrast
Others
AxesZ (Sec.)Y (Row.)X (Col.)
0.79 Å/pix.
x 340 pix.
= 268.6 Å		0.79 Å/pix.
x 340 pix.
= 268.6 Å		0.79 Å/pix.
x 340 pix.
= 268.6 Å

Surface

Projections

Slices (1/3)

Slices (1/2)

Slices (2/3)

Images are generated by Spider.

Voxel sizeX=Y=Z: 0.79 Å
Density

Histogram

Histogram (log scale)
Contour LevelBy AUTHOR: 0.2
Minimum - Maximum-0.2993481 - 0.6349319
Average (Standard dev.)0.0000016015553 (±0.021640755)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions340340340
Spacing340340340
CellA=B=C: 268.6 Å
α=β=γ: 90.0 °

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Supplemental data

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Additional map: #1

Fileemd_72382_additional_1.map
Projections & Slices
AxesZYX

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Slices (1/2)
Density Histograms

Histogram

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Half map: #1

Fileemd_72382_half_map_1.map
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Half map: #2

Fileemd_72382_half_map_2.map
Projections & Slices
AxesZYX

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Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Sample components

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Entire : Human antibody Fab MPV499 bound to hMPV DsCav-ES2

EntireName: Human antibody Fab MPV499 bound to hMPV DsCav-ES2
Components
  • Complex: Human antibody Fab MPV499 bound to hMPV DsCav-ES2
    • Protein or peptide: MPV499 bound to hMPV DsCav-Es2 F protein

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Supramolecule #1: Human antibody Fab MPV499 bound to hMPV DsCav-ES2

SupramoleculeName: Human antibody Fab MPV499 bound to hMPV DsCav-ES2 / type: complex / ID: 1 / Parent: 0 / Macromolecule list: all
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 90 KDa

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Macromolecule #1: MPV499 bound to hMPV DsCav-Es2 F protein

MacromoleculeName: MPV499 bound to hMPV DsCav-Es2 F protein / type: protein_or_peptide / ID: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Recombinant expressionOrganism: Homo sapiens (human)
SequenceString: QVQLQESGPG LVKPSQTLSL TCTVSGGSIS SGDYNWNWIR QHAGKGLEWI GYINYSGSTD YNPSLKSRVT ISVDTPKNQF SLKLTSVTAA DTAVYYCARG VDFWSGYCDY WGQGSLVTVS S PXXXXSLX VSPGERATLS CRASQSVGNN LAWYRQKPGQ APRLLIYGAS ...String:
QVQLQESGPG LVKPSQTLSL TCTVSGGSIS SGDYNWNWIR QHAGKGLEWI GYINYSGSTD YNPSLKSRVT ISVDTPKNQF SLKLTSVTAA DTAVYYCARG VDFWSGYCDY WGQGSLVTVS S PXXXXSLX VSPGERATLS CRASQSVGNN LAWYRQKPGQ APRLLIYGAS TRATGIPARF SGSGSGTEFT LTISSLQXED FAVYYCQQYD NWPPYTFGQG TKLEIK

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

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Sample preparation

BufferpH: 7.4
VitrificationCryogen name: ETHANE

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Electron microscopy

MicroscopeTFS KRIOS
Image recordingFilm or detector model: DIRECT ELECTRON APOLLO (4k x 4k) / Average electron dose: 60.0 e/Å2
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Nominal defocus max: 2.5 µm / Nominal defocus min: 1.2 µm
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

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Image processing

CTF correctionType: NONE
Startup modelType of model: EMDB MAP
EMDB ID:

5995

Final reconstructionResolution.type: BY AUTHOR / Resolution: 6.57 Å / Resolution method: FSC 0.143 CUT-OFF / Software - Name: cryoSPARC / Number images used: 22000
Initial angle assignmentType: NOT APPLICABLE
Final angle assignmentType: NOT APPLICABLE

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