EMDB-52843: Cryo-EM structure of the kinetoplastid trans-spliceosome activate...

Basic information

Entry

Database: EMDB / ID: EMD-52843

• Title: Cryo-EM structure of the kinetoplastid trans-spliceosome activated for catalytic step II (C* complex)
• Map data: Composite map

Sample

• Complex: Leishmania tarentolae trans-spliceosome activated for catalytic step II (C* complex)
• Protein or peptide: x 53 types
• RNA: x 6 types
• Ligand: x 8 types

• Keywords: Trans-spliceosome / Spliceosome / SPLICING

Function / homology

Function:

U2-type post-spliceosomal complex / spliceosomal complex disassembly / exon-exon junction complex / pre-mRNA 3'-splice site binding / Lsm1-7-Pat1 complex / U6 snRNP / generation of catalytic spliceosome for first transesterification step / U12-type spliceosomal complex / pICln-Sm protein complex / snRNP binding / small nuclear ribonucleoprotein complex / SMN-Sm protein complex / spliceosomal tri-snRNP complex / P granule / post-mRNA release spliceosomal complex / U2-type catalytic step 2 spliceosome / U1 snRNP / U2 snRNP / U4 snRNP / U2-type prespliceosome / U2-type catalytic step 1 spliceosome / generation of catalytic spliceosome for second transesterification step / spliceosomal complex assembly / pre-mRNA intronic binding / precatalytic spliceosome / Prp19 complex / U2 snRNA binding / U5 snRNA binding / spliceosomal tri-snRNP complex assembly / U5 snRNP / U1 snRNA binding / RNA splicing / U6 snRNA binding / mRNA cis splicing, via spliceosome / nuclear-transcribed mRNA catabolic process / U4/U6 x U5 tri-snRNP complex / RNA processing / spliceosomal snRNP assembly / catalytic step 2 spliceosome / protein K63-linked ubiquitination / mRNA processing / spliceosomal complex / peptidylprolyl isomerase / peptidyl-prolyl cis-trans isomerase activity / mRNA splicing, via spliceosome / RING-type E3 ubiquitin transferase / regulation of gene expression / metallopeptidase activity / ubiquitin protein ligase activity / nucleic acid binding / RNA helicase activity / RNA helicase / mRNA binding / chromatin binding / GTPase activity / GTP binding / RNA binding / ATP binding / nucleus / cytosol / cytoplasm

Domain/homology:

Zinc knuckle / Pre-mRNA-splicing factor Cwc21 C-terminal / NF-kappa-B-activating protein, C-terminal / NF-kappa-B-activating protein / NF-kappa-B-activating protein C-terminal domain / Cactin, C-terminal / Cactus-binding C-terminus of cactin protein / Prp18 / Pre-mRNA-splicing factor 18 / Prp18 domain / Pre-mRNA-splicing factor SLU7 / Mago nashi protein / Mago nashi superfamily / Mago nashi protein / cwf21 / Pre-mRNA-splicing factor Isy1 / Pre-mRNA-splicing factor Isy1 superfamily / Isy1-like splicing family / Sm-like protein Lsm4 / U6 snRNA-associated Sm-like protein Lsm1/8 / Helix hairpin bin domain superfamily / : / Cactus-binding C-terminus of cactin protein / mRNA splicing factor Cwf21 domain / Pre-mRNA-processing factor 17 / Pre-mRNA-splicing factor 19 / Pre-mRNA-processing factor 19 / : / : / Prp19/Pso4-like / Cyclophilin-type peptidyl-prolyl cis-trans isomerase, cyclophilin A-like / Myb-like domain profile. / : / Pre-mRNA-splicing factor Syf1/CRNKL1 C-terminal HAT repeat / U-box domain / WD repeat Prp46/PLRG1-like / G10 protein / : / Pre-mRNA-splicing factor BUD31 / Initiation factor eIF-4 gamma, MA3 / : / : / MA3 domain / Pre-mRNA-splicing factor Syf1/CNRKL1 N-terminal HAT repeat / MI domain profile. / Domain in DAP-5, eIF4G, MA-3 and other proteins. / MIF4G domain / U2 small nuclear ribonucleoprotein A' / Middle domain of eukaryotic initiation factor 4G (eIF4G) / MIF4G-like, type 3 / Brr2, N-terminal helicase PWI domain / N-terminal helicase PWI domain / : / Winged Helix-turn-helix domain / Sec63 Brl domain / U-box domain profile. / Leucine-rich repeat / : / Pre-mRNA-splicing factor Syf1-like / Snu114, GTP-binding domain / Modified RING finger domain / U-box domain / Sec63 domain / Sec63 Brl domain / Small nuclear ribonucleoprotein Sm D2 / Small nuclear ribonucleoprotein Sm D3 / Small nuclear ribonucleoprotein E / Small nuclear ribonucleoprotein F / : / Nucleolar protein 10-like, N-terminal domain / : / Helicase associated domain (HA2), ratchet-like / DEAD-box helicase, OB fold / Oligonucleotide/oligosaccharide-binding (OB)-fold / Helicase associated domain (HA2), winged-helix / Helicase-associated domain / Sm-like protein Lsm7/SmG / Helicase associated domain (HA2) Add an annotation / Like-Sm (LSM) domain containing protein, LSm4/SmD1/SmD3 / Sm-like protein Lsm6/SmF / LSM domain / LSM domain, eukaryotic/archaea-type / snRNP Sm proteins / Myb-type HTH DNA-binding domain profile. / DEAD-box subfamily ATP-dependent helicases signature. / HAT (Half-A-TPR) repeat / HAT (Half-A-TPR) repeats / ATP-dependent RNA helicase DEAD-box, conserved site / RNA helicase, DEAD-box type, Q motif / Myb domain / : / Sm domain profile. / DEAD-box RNA helicase Q motif profile. / Myb-like DNA-binding domain / Elongation Factor G, domain II / Elongation Factor G, domain III / DNA/RNA helicase, ATP-dependent, DEAH-box type, conserved site / DEAH-box subfamily ATP-dependent helicases signature. / LSM domain superfamily / Translation elongation factor EFG/EF2, domain IV

Component:

CWF21 domain-containing protein / Sm domain-containing protein / Sm domain-containing protein / Pre-mRNA-splicing factor SLU7 / G10 protein / Uncharacterized protein / Cell division control protein / ATP-dependent RNA helicase, putative / Uncharacterized protein / LSM domain-containing protein / Uncharacterized protein / Pre-mRNA-splicing factor 18 / Pre-mRNA-splicing factor Syf1-like N-terminal HAT-repeats domain-containing protein / Small nuclear ribonucleoprotein, putative / peptidylprolyl isomerase / Lsm7p protein, putative / Sm domain-containing protein / Guanine nucleotide-binding protein subunit beta-like protein / Guanine nucleotide-binding protein subunit beta-like protein / RRM domain-containing protein / Pre-mRNA-processing factor 19 / Small nuclear ribonucleoprotein E / Mago nashi-like protein,putative / U2 small nuclear ribonucleoprotein 40K, putative / Lsm5p, putative / RRM domain-containing protein / Uncharacterized protein / Sm protein G / U6 snRNA-associated Sm-like protein LSm4 / Sm protein B / Small nuclear ribonucleoprotein Sm D2 / LSM domain-containing protein / Small nuclear ribonucleoprotein component-like protein / Probable eukaryotic initiation factor 4A / Uncharacterized protein / Splicing factor Cactin C-terminal domain-containing protein / Zinc knuckle / Prp8 protein homologue, putative / Small nuclear ribonucleoprotein Sm D3 / Sm protein F / Pre-mRNA-splicing factor Syf1/CRNKL1-like C-terminal HAT-repeats domain-containing protein / RNA helicase / Nucleolar protein 10-like N-terminal domain-containing protein / Unspecified product / Uncharacterized protein / MI domain-containing protein / NF-kappa-B-activating protein C-terminal domain-containing protein / Small nuclear ribonucleaoprotein, putative

• Biological species: Leishmania tarentolae (eukaryote)
• Method: single particle reconstruction / cryo EM / Resolution: 2.7 Å
• Authors: Nadenoen T / Vanden Broeck A

Funding support

Belgium, European Union, 2 items

Organization	Grant number	Country
Fonds National de la Recherche Scientifique (FNRS)	MISU F.6005.25	Belgium
European Research Council (ERC)	TranSplice 101162011	European Union

• Citation: Journal: Nat Commun / Year: 2026; Title: Structural basis of step II spliced leader RNA trans-splicing in trypanosomatid parasites.; Authors: Théo Nadenoen / Franco Agustín Biglione / Marylène Vandevenne / Arnaud Vanden Broeck / ; Abstract: Messenger RNA (mRNA) synthesis is fundamental to eukaryotic gene expression. In kinetoplastid parasites, including the human pathogens Trypanosoma and Leishmania, mRNAs are produced through a mechanism called Spliced Leader (SL) RNA trans-splicing. In this process, a short SL exon from a small noncoding RNA is joined to the 5' end of every mRNA, ensuring transcript stability and translation. Despite decades of study, the structural and mechanistic basis of SL trans-splicing remains elusive. Here, we report cryogenic electron microscopy structures of step II SL trans-splicing machineries from Leishmania tarentolae. These structures reveal the molecular mechanism of SL exon ligation and uncover lineage-specific adaptations that remodel the trypanosomatid trans-spliceosome for SL trans-splicing while preserving fundamental spliceosomal chemistry. Altogether, our results establish a mechanistic framework for SL trans-splicing and illuminate the evolutionary diversification of RNA processing in deeply diverged eukaryotes.

History

Deposition	Feb 17, 2025	-
Header (metadata) release	Sep 2, 2026	-
Map release	Sep 2, 2026	-
Update	Oct 7, 2026	-
Current status	Oct 7, 2026	Processing site: PDBe / Status: Released

Map

• File: Download / File: emd_52843.map.gz / Format: CCP4 / Size: 244.1 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
• Annotation: Composite map
• Voxel size: X=Y=Z: 1.065 Å

Density

Contour Level	By AUTHOR: 0.7
Minimum - Maximum	0.0 - 9.610611
Average (Standard dev.)	0.12035669 (±0.21018909)

• Symmetry: Space group: 1

Details

EMDB XML:

Map geometry	Axis order X Y Z Origin 0 0 0 Dimensions 400 400 400 Spacing 400 400 400
Cell	A=B=C: 426.00003 Å α=β=γ: 90.0 °

Supplemental data

Sample components

Entire : Leishmania tarentolae trans-spliceosome activated for catalytic s...

• Entire: Name: Leishmania tarentolae trans-spliceosome activated for catalytic step II (C* complex)

Components

• Complex: Leishmania tarentolae trans-spliceosome activated for catalytic step II (C* complex)
• Protein or peptide: Probable eukaryotic initiation factor 4A
• Protein or peptide: RRM domain-containing protein
• Protein or peptide: Mago nashi-like protein,putative
• Protein or peptide: Prp8 protein homologue, putative
• Protein or peptide: ATP-dependent RNA helicase, putative
• Protein or peptide: Small nuclear ribonucleoprotein component-like protein
• Protein or peptide: PRKR-interacting protein 1
• Protein or peptide: Splicing factor Cactin C-terminal domain-containing protein
• Protein or peptide: RNA-binding motif protein
• Protein or peptide: Splicing regulator SDE2-like protein
• Protein or peptide: Guanine nucleotide-binding protein subunit beta-like protein
• Protein or peptide: G10 protein
• Protein or peptide: Guanine nucleotide-binding protein subunit beta-like protein
• Protein or peptide: Spliceosome-associated CWC15-like protein
• Protein or peptide: Cell division control protein
• Protein or peptide: CWF21 domain-containing protein
• Protein or peptide: Crooked neck-like protein 1
• Protein or peptide: Pre-mRNA-splicing factor SYF1
• Protein or peptide: RNA helicase
• Protein or peptide: U2 small nuclear ribonucleoprotein 40K, putative
• Protein or peptide: RRM domain-containing protein
• Protein or peptide: NF-kappa-B-activating protein C-terminal domain-containing protein
• Protein or peptide: MI domain-containing protein
• Protein or peptide: Pre-mRNA-splicing factor SLU7
• Protein or peptide: Pre-mRNA-splicing factor 18
• Protein or peptide: Uncharacterized protein
• Protein or peptide: PRP19-related complex protein 3
• Protein or peptide: Unspecified product
• Protein or peptide: SNW domain-containing protein 1
• Protein or peptide: peptidylprolyl isomerase
• Protein or peptide: Guanine nucleotide-binding protein subunit beta-like protein
• Protein or peptide: Pre-mRNA-splicing factor ISY1 homolog
• Protein or peptide: Zinc knuckle
• Protein or peptide: Pre-mRNA-splicing factor SPF27
• Protein or peptide: Pre-mRNA-processing factor 19
• Protein or peptide: Probable RNA helicase
• Protein or peptide: Unidentified protein
• Protein or peptide: LSM domain-containing protein
• Protein or peptide: Sm domain-containing protein
• Protein or peptide: U6 snRNA-associated Sm-like protein LSm4
• Protein or peptide: LSM domain-containing protein
• Protein or peptide: Sm domain-containing protein
• Protein or peptide: Lsm7p protein, putative
• Protein or peptide: Sm domain-containing protein
• Protein or peptide: Small nuclear ribonucleoprotein, putative
• Protein or peptide: Small nuclear ribonucleoprotein Sm D2
• Protein or peptide: Lsm5p, putative
• Protein or peptide: Small nuclear ribonucleaoprotein, putative
• Protein or peptide: Small nuclear ribonucleoprotein E
• Protein or peptide: Sm protein F
• Protein or peptide: Sm protein G
• Protein or peptide: Small nuclear ribonucleoprotein Sm D3
• Protein or peptide: Sm protein B
• RNA: Y-branched pre-mRNA
• RNA: SL RNA exon
• RNA: SL RNA outron
• RNA: U2 snRNA
• RNA: U5 snRNA
• RNA: U6 snRNA
• Ligand: MAGNESIUM ION
• Ligand: ADENOSINE-5'-DIPHOSPHATE
• Ligand: POTASSIUM ION
• Ligand: INOSITOL HEXAKISPHOSPHATE
• Ligand: GUANOSINE-5'-TRIPHOSPHATE
• Ligand: ZINC ION
• Ligand: 7-METHYL-GUANOSINE-5'-TRIPHOSPHATE-5'-GUANOSINE
• Ligand: 5'-O-[(S)-hydroxy{[(R)-hydroxy{[(S)-hydroxy(methoxy)phosphoryl]oxy}phosphoryl]oxy}phosphoryl]guanosine

Supramolecule #1: Leishmania tarentolae trans-spliceosome activated for catalytic s...

• Supramolecule: Name: Leishmania tarentolae trans-spliceosome activated for catalytic step II (C* complex); type: complex / ID: 1 / Parent: 0
• Source (natural): Organism: Leishmania tarentolae (eukaryote)

Macromolecule #1: Probable eukaryotic initiation factor 4A

• Macromolecule: Name: Probable eukaryotic initiation factor 4A / type: protein_or_peptide / ID: 1 / Number of copies: 1 / Enantiomer: LEVO / EC number: RNA helicase
• Source (natural): Organism: Leishmania tarentolae (eukaryote)
• Molecular weight: Theoretical: 44.002844 KDa

Sequence

String:

METEQVDNIQ ANVLAIPTFE AMGLKEDLLK GMYSFGYKQP TAIQKRFIMP FLKGRDVIAQ ASSGTGKTSA FCVCLLQACD PHTRETQAL ILSPTRELAV QTQDLCNNIG HHMGLTAYAC IGGKSTEEDI RRLDSGVHIV SGTPGRVFDM IRRKSLRVNG L KTLVLDEA DEMLGKGFKA QIHDIYRMVP PLQIILVSAT LPADVLEMTE KFMTEPASIL VKRDEITVDS VKQYFVAVDE EK NKFDVLM ELYDSLTIAH AVVFCNTRKK VEQLAKKMTR EKFAVTAMHG DMPQAERDEI MRQFRHGHSR VLITTDLWAR GID VERVSL VLSYDLPLAR EQYIHRIGRT GRMGRTGLAI TFVRHDELRL LRDIEQFYAT QIEELPANIG EQV

UniProtKB: Probable eukaryotic initiation factor 4A

Macromolecule #2: RRM domain-containing protein

• Macromolecule: Name: RRM domain-containing protein / type: protein_or_peptide / ID: 2 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Leishmania tarentolae (eukaryote)
• Molecular weight: Theoretical: 17.154041 KDa

Sequence

String:

MEATAAPGTG LRQLRRVRRS RTQYERLQRP DDIQPGYPIP SIEGWVLFFS NLPETTTQED IANYLVSFKE GDPDYFGAIT DIKIPLNSD CFCAGYALVE LEDRAGYERA IAELNGTTFP SGDKPLVVAP TFLGEEEEQD PQPEVVPGEK RVRD

UniProtKB: RRM domain-containing protein

Macromolecule #3: Mago nashi-like protein,putative

• Macromolecule: Name: Mago nashi-like protein,putative / type: protein_or_peptide / ID: 3 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Leishmania tarentolae (eukaryote)
• Molecular weight: Theoretical: 18.684203 KDa

Sequence

String:

MAEQEDLAVA LPAETMESQL YYVRYYSGHT GRYGNEFLEF EISENGSLKY VNNSNYRNDF IIKKQARVSP VVLEEVKKLI VQYSVCESD DERWPAPDRN GRQELEIHFG NTHISLVTNK LTSMADIPKS SEGVSLMRFY SFVRDVKALI FALVSIHFKI K PI

UniProtKB: Mago nashi-like protein,putative

Macromolecule #4: Prp8 protein homologue, putative

• Macromolecule: Name: Prp8 protein homologue, putative / type: protein_or_peptide / ID: 4 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Leishmania tarentolae (eukaryote)
• Molecular weight: Theoretical: 278.149188 KDa

Sequence

String:

MSVSVPPGFE DDDDTVAVDP ALLAEMEEEE QKESRKQQIL TWQRMNTERY GFRAAYRAAV GEKDLMPPEF IRKVVKDNGD LGGKRFKSE RKLCVGMLPY IPLALFKLLE NMPMPWEEAR YVTVVYHVGG VLTIVDDTPT VAEPLYVSQW GSVWTKMRAN K VELLQEGG GFRRAVHKGD ENEPPLDFAD YLMDRVPPPP VLDDLDEEDN AGIVGWFYDP FPRLVAPNQI RGSRRPNGYY FT LDVMEAL YRNASPILPT LDDRNYFYLW DLKSFYAAKA MHLSIPRGPK FEAPPSICTK KDEDDDWTEF NDLRRIIHRD NPR KPRFTM LTERQIAFPF LYGSVVDGVE LAPYHYPATV RVENDDPELP CFTFHPSLNA IKAVEKKYTD VPHSQAALCS STWH RADCQ CLENAADVSL ELPDDFEPFL SALPLEQPNT KTALALLFAP PPYNSFEGGM KRRIDVPLCD KWYQDPPDLL TTDDR DKIL RSYTQLLKHH VKRSLQHRVR LRSSAAAAAA RAEGAVTMDM NAMQEKSRLE KMAEMRYFSK TRMEWLEAAL QVMRQG HNM LVQLINMKCL PYVHIDYNFE AKPTRTLTTK EIKKSRLGPA FHMVRELLGF MKRLVDMHVM YRLGSIDALQ LADATQY LF SHVGVLTGVY RYKLRAMRQI KRSRDLKHML YSKFNVGGVP SGPGNGFWGP AWRVWVFFMR GMTPLLQRYL SNLTDRVL H GRLQKGKTAG KKITRQRIEP DKDVNIKEAF RRELREMLPD NVKASVIVTM DQHMNEAFRH WRAGLPWSVP GLAKPLTDL VCKYVKLRSE EYIRTTQLQR RRISEGDTVD KQAFMKNLGR LTRLKLMDEQ RRQRRYMTGE DASTIMAPTE ATEIYRMMAN WLNDRGFKK ISFPDPSKTA ELSLLQLSLN RLRDQHNIAN RLTANQREEQ ARIEESFNAP HEALSRIVDA LAHQRRFKNV E VEYMDNFS HLYPLYTVVP QDKMVDSFLD QYLWYKAMNV QRLFPNWVKP SDVEPLPQLV YKWCEGINNI PDIWDVSHDE SV VLLHSNL QDAFYDNVDW NFFRPMLELI MDKSLVDYIV SRHDVVVEFK DMSYHQYKGL IRGFMFSSFL AQYWGLVMDV LLL GTQRSK EIAGTALKPN PFMSFLRDPY LATSHPIRAY CRYKNEVYIV LRYTKTEADE VRRRYLEETK SDPEMRAINA SVYG FKNAK EWPRDARMRL FLSDVNLARA VLWEFRSRLP PSMATINDAN SFVSVYSKDN PNLLFDMAGF SVRLLPVVRT EADIL ESES MWSLRNHTTK DISCRAFLQV TKEHINIIRN KARRTVMMVG SSTFHSIAAK WNALITEIVP YYREAILGTE ELQAIL ARA EHRMQARVMM ALNSRAKSRF PPAMFYAPSD LGGLGMLSVG HSLIPAKDTI YSKTTSTGVQ FFYAGLTNEE GIPIPNV LQ YYTPWETEVK ESEKAWLEFR TREREAKARG GRVSLDDIED IIDKGIPRIR VRFSRHAPLF HFDTGFRARM EFQRYLAG K YLKNWWFHME HDGNICGGVM ERYRADMNNA LGGVEAILEH SLFKGTGFPS WEGIEFDRSG GFENSKKDTK LAKQQRAGL SKVPNQRFAL WWSPTINRSD IQAGFESKVE TTGVFMCGKL ETIKKSLIKI FSGSLWEKSH GAVVNDLASK LKDSLMDLGA ASITLQQQH PQKSYTFTSS AADVILVSAA RWSVSSKPTS LSDEVGDVYQ HNTTSKFWID VQLRWGNYDS HNIAEYARKK F YEYSTARM YPFPAGIVVA IDLAYNCHSA FGYWIPGMKP FVTKLMPAIM KNNITLNTLR DRMKRDLGLF SSAPTEASLS DT NIAELFS SGMRTWIVDD SATYVTSEQP TPDGGKKFKS ENGAVLVFEP VSGSLKMSVV HRSVFSGQKR RSKLAREKAA EEI ASWLRS CPPSERPSKI IVTRSRFRQT LHNMLVLDYP NIIIGQSDLN LPLPMVLRHS RLVELRIAAM ESKGWEFCIY DDWQ QSLQF QPITCFNLLN LVLRGYHINL QRTRQILVPD LHVEVSVSHF WPTYATRQEW EQVSIRLQEM IIADAARRMN VSPKD FTEK EKEGILLGKR MANVEIQQEE MKEIEAMRRT KLTETQTVNV VTSSGDVVKR KVKAAFDFGA TALNNNWRPR SLADAA FLD ENTPITFDAA GATGASDQLI FSEDVIQKLL ACCDVKVQCC AYMFGHALPD SPNIKEVLCV MIPPQFGTAV EARTPPR IP FGAAALQEAN LSFLGLMRIG ESEAQLTSHD LALQARALIT NEGMVPQGFV TAVLEMSEEG VVVRCYSTTA DGIAWAQS E YEGLLKKTPA ATDPSFSSPC RGSLSSEARG FFLVPADRVW NYFFKGALWR ENTEYDVVVD VPLPFFDALH RPDHFLNFT RIGDGSELVD EADPNDVFEN ESGLRGE

UniProtKB: Prp8 protein homologue, putative

Macromolecule #5: ATP-dependent RNA helicase, putative

• Macromolecule: Name: ATP-dependent RNA helicase, putative / type: protein_or_peptide / ID: 5 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Leishmania tarentolae (eukaryote)
• Molecular weight: Theoretical: 261.270281 KDa

Sequence

String:

MHFVNRLGVS SLATHYVPHS DLTKSHYERL LLDVRRYLPP GVEDDVEEIA EQVLDIVCST ASGASSSAGT LNEQHQSLER LLDTRLSRD ARDHLLQYGK LITDYVSSDK AEEANGKYGL PASSSGALGV QDLNFAGPSS SDSDSDNSDD AARSDAEERR N VELLTGGA SSSAAERKER RRKRKALMKA DQNLTQYAFA EEGGIADTVE EAQAWRDAAP PGADGTAGTE MSGDEYDEDG AL TRGAVDT PRIPFEEVSC NAQFLKDTVR RLFPAHDADT CHHLAQRVLN FLSEKEEDDF TLETQLTTCL GGYEDEAVMD WIG DVVQSR WSVVYGLRYA QCATQKERHA VMDSMQHHAL DDPRVEHLYQ SITGKEVDPL NTEQSQQRRE AAAAGGDEEA ASKF ASTAS RQPLLRRVNL QACAFQDERA PHLHVRATVP QGTQRLTYET HDEVLLPPTS SYNTSNPFIQ VGTSFPDWAM PAFAG VEEL NAMQSKVYDC AFHSDENMLV SAPTGAGKTN VAMMAMLRTI AASRNPKTGV IDGHNLKMVY VAPMKALVHE VVRTFS KRL ESLGLTVCEL SGDATMTQQQ MATTQLIVTT PEKWDIVTRK SVELGVASLL KLLIIDEVHL LHNERGPVLE AIVARTM LQ QQLRGEGGIR LVGLSATLPN HADVAAFLQV NRQRGLFVFD NSYRPIPLEQ TYCAIKKIKG MAQSAVMNLV AYDKVLQT V QAEEQVMIFV HSRRETEHTA RYLQKRAAEE RRGYYFVRPD SDSHKALLEA SSGAGGAVLR RSLQQLLPDG FGIHHAGLS RDERNTVEQL FADGHIKVLV CTSTLAWGVN LPANRVIIKG TRVFNGSKGK SELLSALDVL QMFGRAGRAG YGAAVGRATI ITSPDDLHY YLSVLNQQLP IESQMMRRVV DMLNAEITLG HVETVEEGVQ WLQRSYLYVR MRQVPEVYGI RPSASDPLLL H HLANVVHT ACEELKESKM VDYDARSRKV AGTAYGCIAS YCYITVTSMA TYLGLMSNAM QDVELFRVFA SSSEFVNIGV RA EEQAQLK ELLESAPVAV RESRYTPMAK INILLQCYIS QKGLEGLPLM SEMVYVKDSA QRILRALYEI CLVREYGRTA RQF LELYLM TVHRQWAVQS PLRQVRSYLP SKHFDAILPA LERVRVPWEE VRRWSVEDLA EKLSDDRRAQ SAYEAIHAVP HYAV DAAVR PLTRGMLYVD IDILPDFDYV ESLHGRSVCE VVLLIEHTNG RLLHHESLQI PLANVLEKAT YACPPVVVPM VKPAP THLF VRVVSPHWLA ATAFTSVCLL NTLLPPVAAP LREVDQRPPS QDANITSVAE RLAPFQLHTV GEMLFPFQDF TALQSD LID PIFLDHPHNL LVGVPPGGGK TVIAELFVLQ FLLEEALKEK ERASEMQQQN QQEEEVSAAQ STVLLPGKLL YLTSNRD VV HRRALDWRYK FGEVLKQRVV ELAGGEDDGT DTDNAGAVNN AVEVASAAIV LTTGENLIRL VRRGDPALAG VTHIVVDH L HLLRAPEGQA VEECMARLNS EPFLVRRGAG RARVLGLTYP LISTAELGRW LKVSVSHQYN YGVSYRQLRV RMVGMELPG PRSRYESGVI AALKLLRRPS YAAVPTVVFV PTARHARDVA QRILLRCRDN YIAETTAQAT DDPRLAVFLA AGVAYMHKGT SELDALAIQ ELVDTPAVYP TTQTVLPLRL VCAFDAAWRL PAALFTNAIV CCGERLTAFE SDDGERGMSY QDCSAVELMQ M ASRAMNEA VLCTRTPRVW VLGKLLNEPL PLESSLRYAD DFRDTVNTAI AQTRAHNRID VLRVLSSHYF LYHVKSNLHF YG VPTASDV SLYASSFASH VVTSLKELKC IEELQLPRHH VKGDDDANEN GGENSAETGE VQEDEDPHAP LRPTARGMAL AHH CIAVST VENLVRVLPV SLKTEKAALY DSTTYLWQIL SHHCVELTPA HLGDAARVTE AAEYRALHSI ARALPESIGV RYID LDFGA TGTKIYVLVL AYCSRLFPAI YFHAAGSASS VEEDNAATAA FDKHFGHSLY HAAVPPATRD ALCSAVSDEV AQRFV QDLR SILPTVRRVV TAVVELLDGD TQAWAVARLI RFVACLQQQE WDSDPPVAQL PCWRASRWAS VEARQAIQSS CTLQDL QTN RAEAASKLEP ALRGVLTTDD AALVVPRVLA SAAQDAAGVP QVVSISAKGR VEEMNGVLAM VVSVTAQLRW SPPATLK LP EGSNGAECTP QTDFIEDAAP ALKWWVRCVI LHRPSATLRA GPSESTRQLA LRVSTAKGVA GAEATSLYEV SSDLFFSL A RLEAEDLDAV EMCAVLTSMA YCGVEATAAV LFEADGEEEA EDEGNT

UniProtKB: ATP-dependent RNA helicase, putative

Macromolecule #6: Small nuclear ribonucleoprotein component-like protein

• Macromolecule: Name: Small nuclear ribonucleoprotein component-like protein; type: protein_or_peptide / ID: 6 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Leishmania tarentolae (eukaryote)
• Molecular weight: Theoretical: 110.616109 KDa

Sequence

String:

MDFGYDEYGN RTSAGNGDGR RGEEGHAEGR SAQGSRATSP SNSHGAEEDH QRRVLDSSDS ESRSDFSDDV ELVIGEEGTQ ALHQPLVEQ SELQSQGRRG MGWSREAQFL LENRYAGRDG KASSELLLLA GNRRHQRAQD ASASRSYLDI LSTLPDRMRN V VVAGSLHH GKTSVVELLL NERSYHKRQD EVDREMTLKS HVLTTITGGA VLQPTSRQVT VIDTPGHPDL IGETASGMRL AD AVLFCVD AAESLSDHSE RLLRHAIVNE QLPIVLVITK VDRLMIDIKL PPLDAYRKLR MVVDAVNNVI AGCGTTYDTF LVS PERGTV CFSSAKLGLF FSLETFAMKY AVVYPSISAG ALATKLWGQV TYEKKEFKKI VNFRQRPTFV QFVLEPLYKI VAHS VTGEA EQTLSPNLAK LPRSPLAALQ EAMRYFCGAP AGETLDAILS VLPTPLSRSQ WLSEKYGAGA LCSMTRDAVA AAVER GEAG AAAATADDLV IAVASLQRVF DAQDTLAAVV RVVQGTLRVN SSSTSKNTSV PSSRHGDPSR LRLIAFDEFS SDADPY YEV EVQGLYLRTV EDGFVPVQRA TAGQTVYATG LPARSGSHIV LVGGAAVAAV LAEDEGAPSP PEAFGMTSEW VVSIKVR SL GFPHPLLHVS MEVRDPGKAN SVQEGLGVLL RTSPGLDVHK EETGEYTISG FGELQLDTAL HELRHGLCPN VPVGISQP F VTFAETVHDT EGLLAMTGTR NNSVGFVSGA LPRAFTQAIE YEQLRLFSTT LDEDRQPRKL WTVLRRDYGF DALDAQHVL AAGPDGTKGP SILIDDTLEE EAHYPLKAAH QRAIVSAFRS TMAAGPLVGE MVRGVAAKLI FANIDASTRD AVVLSNARTA LRHSLFGAR PRLMEPVMAV EILCAPECVS QLGDILQQRR GTMLGEEFIS ATTLIRARGL VPAMDSFGVE TQIRMLTHGQ A FPLFRFHQ WDVVPGDPFD ANIHVGPLEP ARGHQLARDF VLKTRFRKGL PQNMLTDL

UniProtKB: Small nuclear ribonucleoprotein component-like protein

Macromolecule #7: PRKR-interacting protein 1

• Macromolecule: Name: PRKR-interacting protein 1 / type: protein_or_peptide / ID: 7 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Leishmania tarentolae (eukaryote)
• Molecular weight: Theoretical: 18.26634 KDa

Sequence

String:

MSIPSSSGKS HGGNAPPPLT PEEKAARDAR LQREHESYIR GIATMRSLPR LRSTIAGAGG KTNTNTRIAE EREEVRQQHL RAAAASAAE LQQRRQAHQE KVQKLESTQA KKRKRRAAKK LKKQQRLEEK LRSRLDSHSD SSSACSAFSS SVSASSSNES G RDDVDA

UniProtKB: Uncharacterized protein

Macromolecule #8: Splicing factor Cactin C-terminal domain-containing protein

• Macromolecule: Name: Splicing factor Cactin C-terminal domain-containing protein; type: protein_or_peptide / ID: 8 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Leishmania tarentolae (eukaryote)
• Molecular weight: Theoretical: 78.806461 KDa

Sequence

String:

MDWDRPSGKR RRTDEDEACE ALGGLLRQTA SRAMERVRLQ RQQRGWESQL TILSSSAAAA VVRSVKPMDV LVIRFCLYYL DCSTVAAAN HFVRLAQEVV TSSIERVLVP QLFSRDGSSL PALCEGLVTE HTAEEIQVTV KEVSEFVEAR GALGAWIADA T ATRGDRDD DDYDDYDDAD NYGGGYDDYS RSRDRDDGYS RLTNEEGEGD GEGDHGSPAS PHKDTNDFAL AITLYQACEF WA RVQAFLN SLSLHSAHLH VFAMPPRYER ELLLLLYTVT RGGGDHDEAS VEAVALAGGS ASRVGKGNNA GITPAEGASV SLS ANAVAA KVKKKEAIKQ ESMWDSDDEV AAPVAGENCT DVEGAENTDV VEGPRQPQQL YRASALLADV LALSRCPDVP TASS SPPAG TTSEVALISS PLSAAAPRIS AEATQRLQGL SPKFYKMVYA RLGVMVARDR LHRFDRVSAI TPPEFTVQQR NLQEQ LLQQ EQQQQYKADL MSAALGIPGQ PEAGLPAAYV PDDTYYEKLL HQQHDTIDED EEVYKGEVMD YYLSHPNRPG AATESS SSY SRARSHHRGS SARNGEAEPL MKPHRFCKVK MGFTWTQYNR THYDSRTNPP PRTEMWYEFT LFYPALAHTK RDMRHIF RI EDTPEGPNDQ YCLLVFSVGP PYADVAYRIR KKQWDTRRGG VRISFDQYGR YKLFFRFTNS NYRR

UniProtKB: Splicing factor Cactin C-terminal domain-containing protein

Macromolecule #9: RNA-binding motif protein

• Macromolecule: Name: RNA-binding motif protein / type: protein_or_peptide / ID: 9 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Leishmania tarentolae (eukaryote)
• Molecular weight: Theoretical: 8.723855 KDa

Sequence

String:

MVTVIRIRNL PATYSAEKLL RLCACFGEVT AFTAVGEEGA GGHSAATADV SFAEKSDALA AAQNMEGMEF DGVFLQVFVR QE

Macromolecule #10: Splicing regulator SDE2-like protein

• Macromolecule: Name: Splicing regulator SDE2-like protein / type: protein_or_peptide / ID: 10 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Leishmania tarentolae (eukaryote)
• Molecular weight: Theoretical: 17.569488 KDa

Sequence

String:

MPAGLPRALC CTLLPVQKVI SLTVLQATKD AILEALSKQT DSSPQTFELY VKENNKLVAL RENISSYDSI FALVRCRGGG KGGFRKQLE KKGRGFARAK MKEKRNSTKA APEEEKSQQK KCDTVAVKKR LAKAEPSTTR TLVKQGLAFV INSFQRGNHA

Macromolecule #11: Guanine nucleotide-binding protein subunit beta-like protein

• Macromolecule: Name: Guanine nucleotide-binding protein subunit beta-like protein; type: protein_or_peptide / ID: 11 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Leishmania tarentolae (eukaryote)
• Molecular weight: Theoretical: 55.427645 KDa

Sequence

String:

MNQTSMEKLL AANAAAAQEL RGVGVLNVPA FDVDALLRTP KSNKNDGSAM MSEAMRCRAN VLWRALYPST APPTTAAVAT CSNADGEAD ALPHTDPSRC DVVRQGILTV VSSTSRDAPS KGMAPCSSGV LTHKRGRDRD DVLSVDPEQL KARSALVVSR A AAEEAEAR KYAAPPKWKL SKVLVGHQGW VWATAVEPGN KWFATGSFDA IIKVWDLETG ALKMNLTGHK EAVRAIALSK LS PYMFSGS DDHSVKCWDL ERNEVVREFF GHKSAVHCVA AHPSLDVVIS GSRDKTVRVF DLRSRAVVHT MLGHTDSVMS LVL QQEEPQ VISGGSDGFI YLWDLASGKA IQRLTRHKKP VRGLAFTAAG DALVSCGADE VRVWKLPSGD FVTNASTRVV HDPQ SRAAT KASTSGTDEQ EEYSYRWSCC AMSPRNVLAV GSQDGELAFY DWNIPQPRRV AGRHYAPYQW TKTKSLPGTL RGEGG INAI TYDVSGTRLI TGESDKSVKI WRMRD

UniProtKB: Nucleolar protein 10-like N-terminal domain-containing protein

Macromolecule #12: G10 protein

• Macromolecule: Name: G10 protein / type: protein_or_peptide / ID: 12 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Leishmania tarentolae (eukaryote)
• Molecular weight: Theoretical: 35.590562 KDa

Sequence

String:

MPAPPPSSPS HLAHTSLPPP FPPLLFHLTV ACPNGLPIQH TRRPVEKQKR STMPLIRPGM PKPPKGFDVV LAKLEEYDEQ MKLATQEES KGVVGVTTRP RHNDRSGGCG ARKRPRDDNN AEDGAMVPPA EASSTIAATE SEVGASTAGE DNVEGPEDKP M PPLWRMAA INRARTRYVF LAYYKQHIIS KEVYDYCVDM RLIDGGLARR WRLPGYERLC CTACGVPGAA SLAANITSKY AL RDRQERR LSTTASQQRI HDTATCICRV PAAQRKSKYF VACAVCGCHG CCSADAVKGV TEGDNGEQMS KTREEPPMSA PGQ GETSKQ

UniProtKB: G10 protein

Macromolecule #13: Guanine nucleotide-binding protein subunit beta-like protein

• Macromolecule: Name: Guanine nucleotide-binding protein subunit beta-like protein; type: protein_or_peptide / ID: 13 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Leishmania tarentolae (eukaryote)
• Molecular weight: Theoretical: 33.586711 KDa

Sequence

String:

MALTVTSSSS PPGGAEAVYW NLHGHSEDVL SAAAHGRVCA TGDVDGAILL WRFSGEVPEH VCSMNTGGPA AVDAAWSGAA TLLVAQGDG CATIWDVEKG ARLRIISRYA VRGRCAWPVV NCIEATARDS FVFGGDDGYL VTADKRSDKV VVGAHMGVPL T SATTAGFS LFAGDVCGTL HWFDTRAGSR EMERIVCGST GITSIMADPL HDRVVTYSMS GSVQVIDTQP FALSSADRLL GA RELGVNE RQALLRGAWG PRGDTVVMPN GKGSVVSVPA NDIMGGATRT VHAGRSGSAM NVSVCVEDEY VVCSGDGGEV AAY RW

UniProtKB: Guanine nucleotide-binding protein subunit beta-like protein

Macromolecule #14: Spliceosome-associated CWC15-like protein

• Macromolecule: Name: Spliceosome-associated CWC15-like protein / type: protein_or_peptide / ID: 14 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Leishmania tarentolae (eukaryote)
• Molecular weight: Theoretical: 22.580836 KDa

Sequence

String:

MPLMSGIIHK ETLPGQSAIK RRRLHTQATT NPCEEGENGD DNEKHVADVL KCVAGENDAI SSSVAQCSPL HSADKSSAKA AHVRSSSSD DDEQIERENA RLAKLREQRS RRQREQQSTG DASPASSAQP SANMPDGVST ASCAASKAQE GAVHTNSYDH D VLFRNPAW CNPSKAATPA EKRKQKWDSV LNRTQDSAAF GHFMKNFFK

UniProtKB: Uncharacterized protein

Macromolecule #15: Cell division control protein

• Macromolecule: Name: Cell division control protein / type: protein_or_peptide / ID: 15 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Leishmania tarentolae (eukaryote)
• Molecular weight: Theoretical: 89.110742 KDa

Sequence

String:

MSRLHFKGGA WTNAEDEVMK AALAQYGLRD WERVASMLTK KTAMQCRERW ENYLDPRLNI HEAWSLEEEE KLVELQALFP HKWRLISEQ LTRRTPNHYI RPAWLCEQRY LELKDEQEYY LKQQQHQLKD APGGAAGEKQ SLESFMAERR RRRIARKTHE E RASRADTV SGERSEREMI DMATSRLANQ DQKKGLRKER QQQLAEAAFL AKLESNREGI ESGTLSLRQT RKMEKALEED RQ KPNLVDE IEEAGEEDDT GSEGRDSGEN DEQFEAIDMY EDQKQAGLVK KQRILMKDLT EQQRLIQQRQ INSAADADGD EEA SVSPSG GSRARTALTG VNQDLLKQLT SAPTSASPSL LECNALGTRA ITAAGKPVTG ANSIAAAAAA EAKEELKSTL DNLF ATLPS ASRAAPAPTL TSPSAELQSP FPLPPLKRPR QASRPASVAE GEDGNASHSR EVAQVQVPEV PSRSAGRDSI ETKRT DNNI PEDLFGILDI PAPRKIAKTE ATATAATDSS NGASRSEEPR APIHANADSS DPGASRTSAS NAQYGISFTE SEAEMY MQD ARRWVSVECE RETAASATGT TSDDEPPTPA EIYAAQRLVE QELCSAGCTA VDGTSMANGR GTTRTTQLQH SSSIAEA VR AHTSASGLQA YGAHVNVQQA LHQLSQRVAT QVREANAILR PLRQEWEQVM STAMLSPQDR HALHGFLRDD GQLKGSPS V GDNAEEDEGG GVAPPSVAAY VCERIAGQLQ RARLASRFTK ELRDREVLKM RRDLDEVEHD LQQLQSRERT LQDMYRHKR AQQL

UniProtKB: Cell division control protein

Macromolecule #16: CWF21 domain-containing protein

• Macromolecule: Name: CWF21 domain-containing protein / type: protein_or_peptide / ID: 16 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Leishmania tarentolae (eukaryote)
• Molecular weight: Theoretical: 21.499027 KDa

Sequence

String:

MYNGVEAAAV KGTGLSGYVQ RSRVNVSAAG RAPAEEGAEP VEGGASINPL EQRRAQGENR EMAARLESHH ALRDVRLRVM LYREEREAA GVDPVTIDRE CDELYNSLLV AAKRRLKAQQ KTENAKTAAK FAAAFGVKST ATMTTAGSGG VGSGSAFDRS V QEVEKRRS EEERRRHQEA QLVEHLKRMR REGSDAAAP

UniProtKB: CWF21 domain-containing protein

Macromolecule #17: Crooked neck-like protein 1

• Macromolecule: Name: Crooked neck-like protein 1 / type: protein_or_peptide / ID: 17 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Leishmania tarentolae (eukaryote)
• Molecular weight: Theoretical: 93.722797 KDa

Sequence

String:

MKRSRQDNRR LGPAAAAMRR RDVYRDGRQA EEQVSVQKLF QDAVAMQKFH SPNQSGAGAR VMIHSREELA LYRQKRRAEL EESVRRGFK AIGNWIRYAR WEAQQHDFDR MRAVMERAVP VHGENSNLWR DYAELEESNG FTEHARQVWS RGVTALPSSV D LWVKYLAM EQAAGQDQRV RDVFHRWLAG DAVPPCAYAL AALYEAQQRR QAGCRDILRR CVERFNTPES WLLYGATEQQ VF GDHERAV KVLETAMQAL PDEDIWGLDE CRVPLALAEA HIAARNIVQA RNIFHNALEH VADRPLLLEK VLASYSRFER LHG DGVHSE QAARLCTIQL YEERLRKNKN DFDAYLTLYS LYRDQEGDSQ PCARGQNNTV PETVVGGSAA MMSTALVTSP AAAA SRSRQ VLERALQIPA RDPYGAQQRA VLVMEYARAC EADSELDLAR RTLAQEIKNF PFDAALCPRL WVEAAKIEER HGDTT QARR LLRAGSQVTK DVCLFEEAIQ FEERQRETVI EAMHAAAQET TPPSARELEE ACAPYVVELR TDYQNAIQAF PFSVQW WLR YAKLEEQQQE ILRADALYGA CIRTFNAEAE RVRSFSRRYD LLGHVDAAWA HRIHLHTRRA RALQRQLLRG KVSATEA ET ARAELATRQE ELSRLYTTLL QDVWNAYKHE ALAWFSRYFE TGEGSGTGVL AGAAPKTLPS SLTPATARWS EAVEAVAG Y MERMGASKTG QGAPEGATAA LRGILKSMVE AERHAIRRSL GWTEQTTQFD AVQRAREWGE LLLSPLLEEW SKFEVTHGG SLEAVVASME KPVKRRTRLF KTRP

UniProtKB: Pre-mRNA-splicing factor Syf1/CRNKL1-like C-terminal HAT-repeats domain-containing protein

Macromolecule #18: Pre-mRNA-splicing factor SYF1

• Macromolecule: Name: Pre-mRNA-splicing factor SYF1 / type: protein_or_peptide / ID: 18 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Leishmania tarentolae (eukaryote)
• Molecular weight: Theoretical: 89.299875 KDa

Sequence

String:

MNGDPRVNAL EWEVVKEATT SPKTWAELAR ITAAAPAADP VERANAVTLV YERALRAFSS SYKLWTLYIS YRQHETSKLC GTNEWFQAV REVYERALEE LPKMPMLWVG YMQFVVGSEV PRVTMTRHIL ARALSSLPAT QHHHLWKVAK RWCAMPFVPS A TVRAVWRL YLSFQRSLHA KREYFQVLVQ KGDFNGFLQE CVHLGLPNKR NTGAAEENDL LLSDVSFWET VQTALQGKGW RF TGGIGPL RDLVSLGKQR CASPVELSMA FAVFLYGQGH MQEGRQELRQ LLEEAPEAHT FMSLYHLAVE VEDQLVESFA VDP DVRHLD DDAYKGIVQH LFGDGDPLSH LERLAREFPL RLNQAQLRNT PHNAALWLKR VELVQENVYA GLSTFDDLIA LYRQ AIQQC TAGMSRVDGA AAQLFYSCAQ ALIRGGRLDS AISVLSEGAW CVPFSSAALN VQLVGLLVEV QLMSSTSPLS VRDAL AERL TSGARNGALK RNRNGLLQSS VALPHVIHHP HTWIIWCDVC AAYDSSNTAA WRHLVESLVS SPAFSAEAAC YVAHKA YVC GQESTAAMML ERAVAATGQN PTAQLFVLEQ YLSFLYVRYG QRVPLHQFRE VFSLVQQVTP VAIRIVPSAV IDLHVSC AY MEATIGLYGT AVRMMQEISF VATAALRGRD EHLRVLQAIL EHSITFTEQR RGYDSVRTYC GALVQRLQNT LLLQRVAL H WAAIEKRSGN TAQAHLILDA CGDSQDPSTE HGEVYWRLWE SLCTSVEEFE KVARRRQQAL IRFSKRENAK RAE

UniProtKB: Pre-mRNA-splicing factor Syf1-like N-terminal HAT-repeats domain-containing protein

Macromolecule #19: RNA helicase

• Macromolecule: Name: RNA helicase / type: protein_or_peptide / ID: 19 / Number of copies: 1 / Enantiomer: LEVO / EC number: RNA helicase
• Source (natural): Organism: Leishmania tarentolae (eukaryote)
• Molecular weight: Theoretical: 123.210883 KDa

Sequence

String:

MNAVGSTVGL VDSDDDAIAE QQNMSFLMKL QELYCKTVLY DQLEQMMGHP ATQEESDGYL DDVEGLVKLY YSEMEAAKKM VPKGEEPST AAQREVTEQH ILHSLSVAIN GGAEADEEPD VPISSIQKLY HVLHGCLTTD TVETGLAVGS LLLADDAKRS A EEAARLAY QQQQRHMHGG VDTASMNTST VERKNAMKVS PEEMFRLAQH VRSGLADEAE LLKLRSEDNE ENGDGAQDVE LN DEEPRFL RNMGFSSMLH RRINYRAPLP SQQLRDARTP QHRQAILDRL AQRQQGDTSK LNSMEAVAQM QEKINMDRRV IAR KAQRAQ QQSREYDFGA AISGREGKLV LDEGRYGRRG EALESEEYSV QPTLLHSELN ALPEEGGFRP QDLPTKLAPW MKHS FGNKP RFGLPETMQT IQEQRVSLPI YAKKEALLNF VDAHRVTVLV GETGSGKTTQ IPQYLAEHGY ADRGMIACTQ PRRVA AETL AMRVAEEYGC RLGEEVGYTV RFRDVTSSLT KIKYMTDGML LREALLDDSF QRYSVIILDE AHERSISTDL LFAIVR QAL RKNEVLKVMV TSATLETEKF CAYFGASEPF RIEGRTFPVE TYYLTDPTTD YVRTALQTVM MIHLQEPPGD VLVFFTG QE EIELGGEQLF RWMEMLRRQV STPLPDLMVL PLTATMPQEV QSKVFEPTPP GCRKVVLATN VAETSITITN LYYVVDSG F CKQNIFDAKH GIDQLKVMPV SQAQAKQRSG RAGRIGPGKC YRMYTEKQFT TDMVPETVPD IMRTSLFHVT LQLKAMGLD LLSLELMDCP PKGAIVSALE KLRYLEALDD DGLLTPLGSR MAQLSIDPSQ SKTLLTAVDL GCSEPVLTIV SMLAVQKRGV FYRPRDQQD ASDAARRQFM QPEGDQLTLM AVYDAWVENG MSEDWCKHNF LKHRMLVEAR DTRDQLKEML ARRNQHISHE N DTNLDEVR KSITAGYFFN AARRVDSHTR SYVTLSDRRE VYVHPSSVLI DDPPKYVLYD DLRMTKREYM TELLAIEPKW LV ELAPAFY AKPKEGRLTK EQAAERFTPI LKSWETGSSW RISRLKKQRR

UniProtKB: RNA helicase

Macromolecule #20: U2 small nuclear ribonucleoprotein 40K, putative

• Macromolecule: Name: U2 small nuclear ribonucleoprotein 40K, putative / type: protein_or_peptide / ID: 20 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Leishmania tarentolae (eukaryote)
• Molecular weight: Theoretical: 34.821762 KDa

Sequence

String:

MRLTIDLVRL APQFTNTMLQ REIDLRGLRI SSLDEHVLVL LENNFDAVNL SSNALTALEY FPTKSAVGNS DKDVRMSRVT TLIVHRNEI QRVSVASCVS ALPNVVHFLA DRNRLACVRD LYFLKQWKKL EVVSLEDNPV WESNSNNFDS DKLRAFLVFL C PRLKLINY QRVTKADRER AQKAKEEFKK LVQSWEVPLT PCAATPLQLP CNVEGKKMRK RGRDGRTAAA AAVASNVITP DE PKNGETS ATSVSVNVNA DDDTALQVRL DQLEERLLSD DVTAEEIAQL EEEMTEISER MIAARKRKRH

UniProtKB: U2 small nuclear ribonucleoprotein 40K, putative

Macromolecule #21: RRM domain-containing protein

• Macromolecule: Name: RRM domain-containing protein / type: protein_or_peptide / ID: 21 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Leishmania tarentolae (eukaryote)
• Molecular weight: Theoretical: 13.295335 KDa

Sequence

String:

MDEPKQTLYI RGLPDKSSAN ELRRALYLYC TQFGPVKAVL YSKSKNFYGQ AFVVFSDVGT ATTARRSLHD RMFYGRQIQA FYAHKQAFC VAPAERRRRD LAREKKLLNT IQKKE

UniProtKB: RRM domain-containing protein

Macromolecule #22: NF-kappa-B-activating protein C-terminal domain-containing protein

• Macromolecule: Name: NF-kappa-B-activating protein C-terminal domain-containing protein; type: protein_or_peptide / ID: 22 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Leishmania tarentolae (eukaryote)
• Molecular weight: Theoretical: 43.596113 KDa

Sequence

String:

MSNSVWALAV STTSPSDLPA GDEVEQIVCR SLEENAYSVQ GFWLWQSNFI QSRRRREQRR RRQRNDVASP TKEGSGESAP TRSEAEEAS LQRADEIRGY VSAVLHATGY PYPFASTADD AREGADDPAP STVTDAETAV TSDGLTPASL CSTITDSAAV S GEPPLKVN RGEGTGATAR IPARPASALA KFVAKARAQH CDLVEHAPVV VATVPARWAE LDEPGTQPGM GRKRIQFSDE PP LVKTPQL SSRGHIRRDN GDNDDDDSFT VQLPAHMGLQ SSSNKDKSDG HSMDAGAGGA GGAEAVAAKH PSQMTRAEFL SQF KRAPRR GEVGQTAEEI AAAEKLGYVM SGSRSVASRM YVDRIQRQLH EHEAAKLQQQ FRKVEDERMD DQLVLGLAQF INSK AKS

UniProtKB: NF-kappa-B-activating protein C-terminal domain-containing protein

Macromolecule #23: MI domain-containing protein

• Macromolecule: Name: MI domain-containing protein / type: protein_or_peptide / ID: 23 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Leishmania tarentolae (eukaryote)
• Molecular weight: Theoretical: 65.686367 KDa

Sequence

String:

MSRYVPPHRR QAVASSSEEL SSTSSVAFQQ EAWRALSRSI TGVVNRLNKD NLEQSAVELF RENLIRGRGL LARSMMRTQQ VDPDLTPVL AALTSRINKD LPTVVELLCR RLVVQWNRAY LRKDWRYVEN ASRYLGWLFL LNVVDVDIIY QLLLKHLMSE K RCDEDIDQ AAKLFRETFK VMSLKERRSF HEQILTPFRD LLAMDDEELR LSTRSQAVLE SCLKEVQEWE RRKEKEEAIP DH LILFELE TQQRHEMDLD AKYSTEDTLD RYAFDAEYDT HEEQYEAVRK AILGEDWEVE LLQQVAEAEE DEEKGGDEGD DEA ADGPLA EAGTDVSEAA SFPTGAALDT SKTLIDAEER KLRREVYLAM RSSIRADEAV HKILRQMQPQ TERTICFMVI EGCC EERAY RKMYSMAAER LCKSNARFQA FFVEAFHARY ESASDLTLKQ IEYTCKVYSH LLRTNSVYWS RCLSCLDIVE NNESQ RLMI QYLFKGLAEE IGMPRIMERF QKDEELRWHT QQLFPVNRGV EALKSAVNLY VAMGLPEMST GLRAALEEER AAHKRT RLD Y

UniProtKB: MI domain-containing protein

Macromolecule #24: Pre-mRNA-splicing factor SLU7

• Macromolecule: Name: Pre-mRNA-splicing factor SLU7 / type: protein_or_peptide / ID: 24 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Leishmania tarentolae (eukaryote)
• Molecular weight: Theoretical: 46.469113 KDa

Sequence

String:

MDPNADEPKK ASSVRQLLRQ QRELQEMRKF GLAPLAVDTE THQEISPNIP EFISRAPWYY GATGPTLVHQ RKQGNGATEA PDEVLDEKV DRVVVKGQAT RFTAGACKNC GSRTHKTNEC FQAKKKVGSV YTNRVTGSDI ETHQEPKTYS QKRDRFVGEV G IDLMRGVR QDQEDDSVDV AAPATARHRP EDVFGSRTAQ HGGVEVKELP KYLHNLDQQD SLFFDPKTGS MRANPNAEDS TK TFRGDLE RYRTGDYYNY VESQYRFLTG QSRSFVDFEF DDVMRRAREQ QQQQEQDNTG EPVAKVLTET PQDVLLRSLY GTS TPKFDS DVPPSSTKES KTPVPSSSPA STTDALTATA TATATATAHS SVQVASLSWV PTRNGHTAVY GSYFDPIHFR WGYA CCKKT DKDATGCNAT HA

UniProtKB: Pre-mRNA-splicing factor SLU7

Macromolecule #25: Pre-mRNA-splicing factor 18

• Macromolecule: Name: Pre-mRNA-splicing factor 18 / type: protein_or_peptide / ID: 25 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Leishmania tarentolae (eukaryote)
• Molecular weight: Theoretical: 59.023402 KDa

Sequence

String:

MSFEGLQGLL KKKKQEQRKG VSDGLAAAAV VDTNASAHTH AIASSVATTH KSATPTTPAH ATCSGGSRSG GSPSTAYDPY AAALQSVQT LQQQQEQQGA LSNTTVGQKR PREEMVQEST LVGALHTPNG SSAAEESDNL DIKSGALVST PPNASLGTPA A PSMKSDDD VLAQREMGVL ALQMCLTKLA HYWAAERVST KSLSAAAHLA TATAPASAVL YPQHQLSSAE ALLESGDSRT PS ASLLSMP RALHKYLLDR FLAPNSAAPH ATGLPEMMVV DREFELLAQL KAEMEAELHN EATDSVNTAD TKMHTVSEEQ RQL AELLHA LWYLVALLWL HSLDPHQRNG QTCAQSATMP REGTLAPAQQ GWSHAVYLGL QNALPADVRL LQGRAIAEEV DRWR ALART RQDALELLSY VCSDYHTSAA ASLSTTATDF IIPSDLRKNL HTMVVRHLQQ ERNFMAVRQD YVDITMGTAN WKLGL FSGG EVHMRRSMER VERNRIAHLL NNEHATKLLQ AVRELTIFVE RNALAGASTF FCSACSACTM AV

UniProtKB: Pre-mRNA-splicing factor 18

Macromolecule #26: Uncharacterized protein

• Macromolecule: Name: Uncharacterized protein / type: protein_or_peptide / ID: 26 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Leishmania tarentolae (eukaryote)
• Molecular weight: Theoretical: 27.783264 KDa

Sequence

String:

MSDERRGPPN SHGHLASNVR HSHHSSSQFD QPQQRPYGGA SCNSSAASFN ASYPRRGGAT EERGRGQRPG IHNEQEGRAG QRVWSGERR PFHAGRPNSG PTSLMEDAPR TKLSSYVTPH DAVQDQPAQH GATVVRSGSS ATEGGSILFF SSVQKKGGAF T ASERETYA QLHRSRAIPN VNRGRNAGNV MGSGVSAKSV ARQYADLERD REAIAIEKAK TAVVGSKRSR FALDDSLSED DG EEVADAE VMGKRPKTSS N

UniProtKB: Uncharacterized protein

Macromolecule #27: PRP19-related complex protein 3

• Macromolecule: Name: PRP19-related complex protein 3 / type: protein_or_peptide / ID: 27 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Leishmania tarentolae (eukaryote)
• Molecular weight: Theoretical: 45.326121 KDa

Sequence

String:

MASTSASGPS SSTPLPRQWG SDLLLRKVPF YAQARQYGAA DPDDPQSEGN TLSYGPSSYP LLAAVRSCYE MLNRADVATE TSNLGATTA VVDRSPFSGI DRAAYCLVPP QRKWTSNEGL PADSTSPSSE QSTTLVHPAA AAALLDDTIG PATLTEVLEP W FGIIEDRF CADEHQEQVE ELARSGKQNT RTKALNQSHS GAAGVLKDGD MVKKDSLRVV DAEVSRMLRR KRICERDEET HG AVYTRLE RSILHYSAAT QEEKEVLHST RVATNDPSTE ALVRGEGMTA PSQSQETWGS RMLSLVTSTV AADTASGYPR RSD EEVNQQ QKVLAYLRRV RWIATQWNST TSKIAFKDAE ASVPLWSQMT EAQRVKAEMH HAQGQRRRFR RPRRDGDGGP TGNS VDADA ALRDSLGSA

UniProtKB: Uncharacterized protein

Macromolecule #28: Unspecified product

• Macromolecule: Name: Unspecified product / type: protein_or_peptide / ID: 28 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Leishmania tarentolae (eukaryote)
• Molecular weight: Theoretical: 18.754662 KDa

Sequence

String:

MSTRDQSEVA AAAASTPIPP PSSPSPLPAP SSVGTLPACC PEAFKELYRR GEVPRFGCYD PVYLRQALDT TSTFDSAMHD PEHASFFPP FSLLVRTDAD HSAASSSSLL ASRTADAPAV DEDADQDVAT PTKNRGQHVL GWEHITSAED MWNGPPLTTK V PPASDYER ILLDRAA

UniProtKB: Unspecified product

Macromolecule #29: SNW domain-containing protein 1

• Macromolecule: Name: SNW domain-containing protein 1 / type: protein_or_peptide / ID: 29 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Leishmania tarentolae (eukaryote)
• Molecular weight: Theoretical: 57.655543 KDa

Sequence

String:

MSLQFHDGGS FADCPYIQYP HGMGTQRAPA MVLAAAAAAA VSQVEARVRR LPGTPGSAQV SKGSPLATAC PPSQPSASQA LTVVPHATS EKLYGSCALA TTEVPSTASE TAEEATARTA QMLQETLTAA RQTTEVFDSH SLVVQSRLRA ASNKPNRQAL T LTITAAGG DATGVDAAVS DPGPIVR(SEP)PT KKTSLITEAL RLRPGGGNHN KRQRAGDGGS ASGDLAASTS SSSMMLAA V SEGSTGGRGA AAASASSNTV VVPFCVSSWQ NRKKLIVPVE QRLAQQQDDR LDADGGIGDA VMDLAVAMQQ ASKEVAAEL AEKERAKAEE EERRQAALEA AEAEKARQLL EQKAAELAAH AQRRETREQR MERIRLEREL REQEKLAKQQ QRLRERAATR LNITVEDLD ADEQLLRSVE EASVYANSGS GAMASVAPTT ASSSKEKQEA FGGSSRQVPG NDLEDDGDGT LGGSRKAAVR G GVFASAVI SNEGIQREMQ ALAEVEQQQQ QQHHDGIRLR EDDEASAAGD DDDDDGGSFQ LNKFKKKQRL

Macromolecule #30: peptidylprolyl isomerase

• Macromolecule: Name: peptidylprolyl isomerase / type: protein_or_peptide / ID: 30 / Number of copies: 1 / Enantiomer: LEVO / EC number: peptidylprolyl isomerase
• Source (natural): Organism: Leishmania tarentolae (eukaryote)
• Molecular weight: Theoretical: 20.554562 KDa

Sequence

String:

MERAARVVQL QSTAGPFSIE LYDNFCADSF WQLARSGQLR RLTFRQLLGG FALLGEVDSV QGHLATVSEV DTAAQSPDNV PLLHVGAGL LSCRPTVGAV TASCFLITLS PQPQLDKTHV VFGRVYSGIH TLEQMSHMQV DADFILYSPV AVMKCSSAVL V RGTAPPGT TAKAVPTSCA VKSLYPTSIL ETLE

UniProtKB: peptidylprolyl isomerase

Macromolecule #31: Guanine nucleotide-binding protein subunit beta-like protein

• Macromolecule: Name: Guanine nucleotide-binding protein subunit beta-like protein; type: protein_or_peptide / ID: 31 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Leishmania tarentolae (eukaryote)
• Molecular weight: Theoretical: 68.007312 KDa

Sequence

String:

MRLRRHACGA HLQYHHQLRL FFLCCTPTWA QVNATTRLFS PFPRHPLVGV LLGRMTSPRA HTLMCAPGAG SRRYNHFLCP SSLSDPVSK LALTKRPPLP WMFPTDRPPT PADIARLKAF RADGRAEAEP ATEVYLHTVM KPRNDRVAEL LALYTSPEDA R KAYPDPPP SLLEVSDGTL SANKEKASGA ATGTGGLTSG TIAQALKRAR RENATAALSV SSGAAAAVTR TARPVSSVDG SP TALFVDG VERVNYLGKS FLEPPSSVLE HEQSTKQKVC QPPRTMRGVF KVPEGYETAT KAASAPTSSS ALVDQSGNSG GEQ RAGKAS GVQQLRWAPP AYGHLLFSGD IGGECRLWNS STRQLLATFA AHTQPIKSLE VTTNAAIMST GSVDGTVAMW DVEA GACTH VLTNPERLPV VQHLHHPSDE AHLLLAAVDK KVVLYDVRVG CRKYQREYTG HMGTIFNLTL LSDGSKMLTT SEDRT LRTW DYRSPVQIKQ FADAAMHAIT HVLHHPTQPE FLAAQSLNNK VIVFRDDGGG RLRLLHEREF TGHTISGTRC QLSFSH DGR YLSSGDIGGK LYVWDWATKK LEKSFKAHAQ MLVSHLWHPI EPTKVVTAAW DGHIKSWV

UniProtKB: Guanine nucleotide-binding protein subunit beta-like protein

Macromolecule #32: Pre-mRNA-splicing factor ISY1 homolog

• Macromolecule: Name: Pre-mRNA-splicing factor ISY1 homolog / type: protein_or_peptide / ID: 32 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Leishmania tarentolae (eukaryote)
• Molecular weight: Theoretical: 31.012246 KDa

Sequence

String:

MQDRLREIEG TLARAKERKN TILYKLSKRR QEEERLAKLG VAKLPTIPNE VNDEKVIKYV LFKLKQEIGD KTAQLRDQKL LSIDRDGEA VIRAKNDEVN KLLSRKYQWE SRLAFLAGEP MVPRARKKIF FGCAKELPEA ATTQKRQRVE GTKEPGEDEV A ESSDEEEL VGDEAETEEP LTRNNEYLEG VKWLGSSAAD TDLVRFEREA EAKMRAKGDT ALSRSGTKTL ILSYRKEGEI VI PSEEHFK QELVNNRKRE LKERLNALRE KS

UniProtKB: Uncharacterized protein

Macromolecule #33: Zinc knuckle

• Macromolecule: Name: Zinc knuckle / type: protein_or_peptide / ID: 33 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Leishmania tarentolae (eukaryote)
• Molecular weight: Theoretical: 16.757025 KDa

Sequence

String:

(ACE)MYGDKGPPK GYSVPRFDTN AGKRCQTCSN TEHWTFECPR KKSATLDAKK SASKTVKLSP SQMLRYGIKR KSANFV PEP TEREVFDAEL KELRNVLAAE VREEIKAHKA AAHKIGEDGK MHEAQALSAE PEKVQPSEPI TIKKERTEEG E

UniProtKB: Zinc knuckle

Macromolecule #34: Pre-mRNA-splicing factor SPF27

• Macromolecule: Name: Pre-mRNA-splicing factor SPF27 / type: protein_or_peptide / ID: 34 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Leishmania tarentolae (eukaryote)
• Molecular weight: Theoretical: 31.636879 KDa

Sequence

String:

MFDGVVLPFG DAPLGRASST AEEVRWMAFA RDLVLQELQS TPSTAQEWAD ATASSQVAQR GHYQTYLDFI RHMQERLPCD IGLGSPSPI SGAVRPTPGR YRCGPLDRVE VAEPPASLRC AATPSVSTRC APRLRIHEGP MNEDGEKEEP LLSSSSTSLT D GMTQMVAS QTSLDGIFFE GLLSQELNLA LWRRCSLPQE ALRCALEEVQ RLDRAACEED AVLRYLNTMR FTEQRAYKRR RA ELQSQLN KTREKVVALE RLVGVETAGL VRREVGTGER LRACMLE

UniProtKB: Uncharacterized protein

Macromolecule #35: Pre-mRNA-processing factor 19

• Macromolecule: Name: Pre-mRNA-processing factor 19 / type: protein_or_peptide / ID: 35 / Number of copies: 4 / Enantiomer: LEVO / EC number: RING-type E3 ubiquitin transferase
• Source (natural): Organism: Leishmania tarentolae (eukaryote)
• Molecular weight: Theoretical: 53.716508 KDa

Sequence

String:

MLRCNISQRV PTHPVVSTKS GLVFERSLIE KYLDEHGRCP VTGDPLHKED LMTVQGAAPD VSIVSSGGLG AASVPTLLER LQMEWEGVA LEQFSLRQQL TQLQLELAHA LQQYDAACRV IARLSKELDS LRGGKGVAAE EADKGPDAVV VPAAVVRRMD A AEKAARHA RKQRKDAAGS AASAPSKLTE EAQWMLTSSS SVSASSVRSA VRLAAAAENS SPGVFVSAIT GDHAIVRYSS DG FSEVARG IGHSAAVHTL VSQPGDILLS AAEDQTVRVW STKGAALTVM HTLRYASGVA AMSQRTVDGA YILSGAADGV LTF SDLESG VHVVETAPLT LDTASRALTC VELHPYSSLA AVAFQPTELQ LWDARELRAD TFISHVRGAA PRTHICSASF SADC VSLAA GLTDGSVYVW DLRQVSAPVA MLPPNTEAIP ATVRYAPDGR SLIVGGRGVV LYDCARLSVS SGAVDAVATA GLQTG GVCD VCWTTSGRDV LCGTVDGVIR LYSTAASAG

UniProtKB: Pre-mRNA-processing factor 19

Macromolecule #36: Probable RNA helicase

• Macromolecule: Name: Probable RNA helicase / type: protein_or_peptide / ID: 36 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Leishmania tarentolae (eukaryote)
• Molecular weight: Theoretical: 128.35375 KDa

Sequence

String:

MDFSFPHFTR ASTDMVYTQP PLKSLLELVI EAKGRLGLVP QRDVVRHHPS SSPFSHFSDS DEEGEGADRN ANKAEVRQDA VLTTRTSWV PPSYLLDYFF SGEFLSVFLT LHGISASRPV SMLVPPLFTV RDAQLHPLVE AGLCSVLQDA LHSSSRQHRS G GGGGWREG VRGGRGGGGD GGGEGSYMES NAAGTSGGGP FMPYWKGPRQ HHLQGGVLGA GEGNDNEAAE QSASGAAHPR CV YAQHRVD DPAFILPPQR FLFRGLQQVA LPALLLGDHV LMGGPRGIGK RTAALLSVGA NVLAFTSNVD SGTAEETDAP PAL VEGEGG AAADGASAAV TGPVPISPAD AEALQQEAVE DTRDAATSRP EPVSPPAPRA LIVLSSYPEV LSAAHWLRSV FGAE AFTPY TFEKEGTALR PLFEVDTAEQ QPAALDGGSK AASSSDAFGH VGAMNTFPET TGTRRFIGPP VSLPFITDTI GAARA PELL LVDPTQTDSA PLHSETTAPA LAELAALVMN VPLPAQAADT AQATAGERAS GGDSKLKRRR HREDEVDSDV EADEEH VET RSSRTHRRHH RHRSERDEVE SEDDEIRRGR HREKRSPHSR SRHRHHSRHG DRRDRREEEG SRGERNASGH RSHRRHH RR SYESDDSDSD RDKGKWPIED EDTHSPRHSR HQRGCRDHRH HSSGGSVSRR HRSRSDERRS RHGSSRHRHH RSRHHRDH H DEDQRSVSSS STATSSPSSP GFQLARPPSL MPTNVEAELP MMPGNHVAPP AADELRALAL DSIVPPAVAN ETSAPSEKP QEAAEEDRRA ELSDVLHQRI PLLITTYHAL GMALGMVQGE ASALPPLEHV RVALLANLER VLMPPLKMSF AHSWWLSLVN ALDVECQFV VTADRMGNEV RGFLDSTIIP DAGERFVHFD QRDASIWAMM NVQVCAVPVD VPVSAAPPNG GSEGRPRISG S DIDAAKVA HVFSTIAEHM LRTGGHATHG KDDVAFQADC GGRGACVAVV CSTRREQSLV VTQLQRLFQD QANDTQAAKG VR VTEKVEL FRAAEAEVLV LTDAQLADPR VLRDLHQCTE VDLILHFSLS RTVMAQLKKE EIIDLLAQRG RAILGGSKQF CAR RWWRGT AMTSAAGGGA AFGVPSNAVD FEPVKVECQL LLTEHNVHGR VGSCVMEALR EVSDS

Macromolecule #37: Unidentified protein

• Macromolecule: Name: Unidentified protein / type: protein_or_peptide / ID: 37 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Leishmania tarentolae (eukaryote)
• Molecular weight: Theoretical: 1.805216 KDa

Sequence

String:

(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)

Macromolecule #38: LSM domain-containing protein

• Macromolecule: Name: LSM domain-containing protein / type: protein_or_peptide / ID: 38 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Leishmania tarentolae (eukaryote)
• Molecular weight: Theoretical: 20.523877 KDa

Sequence

String:

MVFAGAVYSN ASHVCPHFTL APPIAAFPLL ARPGASHLQR RLGHFALPWP AAHKGAPTLT LERMSESLTT LPGVLARGPA LHALVRVEL TNGTMVVGRL LEMDAATMNI KVDAITHTAV RRRCGRTRKD APAARMAEDT IEVNPIALRC IQSIVVRGTC I RYLDFIHE TKDSGTSYGE VLVAAEQVRP SG

UniProtKB: LSM domain-containing protein

Macromolecule #39: Sm domain-containing protein

• Macromolecule: Name: Sm domain-containing protein / type: protein_or_peptide / ID: 39 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Leishmania tarentolae (eukaryote)
• Molecular weight: Theoretical: 14.153898 KDa

Sequence

String:

MDSPAGCDLG VGPSSSDAKV PGVGTSSSSA PADPFKYLAE HIHKRVSISL LPVDNDDNGV VVCGTLLSVD DLCNVMLQHW SSTDTMRLN RSVGNGLQQQ PRKRARLDIT GGEDAEESVR LIRGAQIVSI SLLPA

UniProtKB: Sm domain-containing protein

Macromolecule #40: U6 snRNA-associated Sm-like protein LSm4

• Macromolecule: Name: U6 snRNA-associated Sm-like protein LSm4 / type: protein_or_peptide / ID: 40 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Leishmania tarentolae (eukaryote)
• Molecular weight: Theoretical: 12.885872 KDa

Sequence

String:

MSFARRPITP IEILRNCRGK EVSIELADGE TVNGTVMRTD RAMNVVIKQC TRTGSDGESF WKSRECFVRG ASVKNVRMTD SALVAVPVS SKRKSAGGKN TDARKERYTA GRKRPPK

UniProtKB: U6 snRNA-associated Sm-like protein LSm4

Macromolecule #41: LSM domain-containing protein

• Macromolecule: Name: LSM domain-containing protein / type: protein_or_peptide / ID: 41 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Leishmania tarentolae (eukaryote)
• Molecular weight: Theoretical: 15.939741 KDa

Sequence

String:

MSANVNFSTG VQTTKAAPSA QASSPSAKSK SHMILPYEVL FNLVGRRVTV LLTKGSQELE GNLQSVDSDK GDMLLSDVVR YTWAPSRSS DEEGTAVEAA GNGQGTLGGA TEEEGCYTCF GGGQRRELSR CSQAMINSAF VALVTPTLFI PE

UniProtKB: LSM domain-containing protein

Macromolecule #42: Sm domain-containing protein

• Macromolecule: Name: Sm domain-containing protein / type: protein_or_peptide / ID: 42 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Leishmania tarentolae (eukaryote)
• Molecular weight: Theoretical: 8.616604 KDa

Sequence

String:

MSVSLESLLH QSVCCHLAHG EEYRGQLEAL DGAFDVVLSK CTRSRAAPGQ PAEPLIFLRG ENIVYIGFDE SSRGEDGATA

UniProtKB: Sm domain-containing protein

Macromolecule #43: Lsm7p protein, putative

• Macromolecule: Name: Lsm7p protein, putative / type: protein_or_peptide / ID: 43 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Leishmania tarentolae (eukaryote)
• Molecular weight: Theoretical: 9.968572 KDa

Sequence

String:

MSAEAAHVLK KKEGILSLEK YLDRKIVVSQ KAHEVHGVLK GFDNNVNLVL ADAQLWHRNT HIRTIGACVV RGGPVNVILS GDTTVISNP FI

UniProtKB: Lsm7p protein, putative

Macromolecule #44: Sm domain-containing protein

• Macromolecule: Name: Sm domain-containing protein / type: protein_or_peptide / ID: 44 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Leishmania tarentolae (eukaryote)
• Molecular weight: Theoretical: 14.105954 KDa

Sequence

String:

(ACE)MLSQYLRKR VSVVTVDGRN LVGVLHAADQ LLNIVLTSCV ERIGAPYDVD DNAAAEEVST VADEVSTAIN RNDTAR STL PPLEEVQLGV MMLRGADVVS IAVIDVYEEA GTNVKDWRGR DMPPVTAVGK SW

UniProtKB: Sm domain-containing protein

Macromolecule #46: Small nuclear ribonucleoprotein, putative

• Macromolecule: Name: Small nuclear ribonucleoprotein, putative / type: protein_or_peptide / ID: 46 / Number of copies: 3 / Enantiomer: LEVO
• Source (natural): Organism: Leishmania tarentolae (eukaryote)
• Molecular weight: Theoretical: 11.214814 KDa

Sequence

String:

MASVSPSETG GTLITFLQQL RGTLVEIELK NASIVSGEIA YVDANMNTYM SHAKITSKGR NPVEVEEYMV RGSTIRYIIM PESLNTYEI LKQASTKKNG ASK

UniProtKB: Small nuclear ribonucleoprotein, putative

Macromolecule #47: Small nuclear ribonucleoprotein Sm D2

• Macromolecule: Name: Small nuclear ribonucleoprotein Sm D2 / type: protein_or_peptide / ID: 47 / Number of copies: 3 / Enantiomer: LEVO
• Source (natural): Organism: Leishmania tarentolae (eukaryote)
• Molecular weight: Theoretical: 11.798734 KDa

Sequence

String:

MDSEQPKKVP RTETKTKELL RTTVADGPFN LLDTAMKEKK RVFIQCRNSK ALLAHVIAFD KHFNLVLKGV QEITESHGSE QKQRSIENV FLRGESVIFI VKLP

UniProtKB: Small nuclear ribonucleoprotein Sm D2

Macromolecule #48: Lsm5p, putative

• Macromolecule: Name: Lsm5p, putative / type: protein_or_peptide / ID: 48 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Leishmania tarentolae (eukaryote)
• Molecular weight: Theoretical: 13.888849 KDa

Sequence

String:

MTAAAASSAV VAVEKRNLVM YVGNRVKVTL DDASLLTGRL VSLSSCGNLI LTDVERQRIL KRRRNRDGLH ETSRECYAAV LFVRGSSVV SVGYDRGITT DKSVIDHIGG RESSTSRMVQ LANASPSTR

UniProtKB: Lsm5p, putative

Macromolecule #49: Small nuclear ribonucleaoprotein, putative

• Macromolecule: Name: Small nuclear ribonucleaoprotein, putative / type: protein_or_peptide / ID: 49 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Leishmania tarentolae (eukaryote)
• Molecular weight: Theoretical: 16.127685 KDa

Sequence

String:

MADTSNICKD IPSSLVFEAL HSRVTVETVE GDLYIGKLSA FDVTRGNIEL VDVRHQGKDS SYGFYERATV RGSKIRIILL PPEMRAAPS LQWYRESVQQ ELKKSLKHVP SLRPVTRAPR PARIKKAEPK NDKSKQLRRK LR

UniProtKB: Small nuclear ribonucleaoprotein, putative

Macromolecule #50: Small nuclear ribonucleoprotein E

• Macromolecule: Name: Small nuclear ribonucleoprotein E / type: protein_or_peptide / ID: 50 / Number of copies: 3 / Enantiomer: LEVO
• Source (natural): Organism: Leishmania tarentolae (eukaryote)
• Molecular weight: Theoretical: 9.926351 KDa

Sequence

String:

MSVHTRQMEN PTGVVHRFMQ DHQRVCVWLV HDPQTRLEGN LLGYDEFMNV VLGDTTETNL RNNKTYRLGK ILLRSDNVGV IHPIGV

UniProtKB: Small nuclear ribonucleoprotein E

Macromolecule #51: Sm protein F

• Macromolecule: Name: Sm protein F / type: protein_or_peptide / ID: 51 / Number of copies: 3 / Enantiomer: LEVO
• Source (natural): Organism: Leishmania tarentolae (eukaryote)
• Molecular weight: Theoretical: 8.533749 KDa

Sequence

String:

MEANVPGAFL HSLVGKKVFV KSKWGPVYEG NLVSCDTFMN LQLRNAVEHA KQDTELGEML LRNNNILYIR EEASAS

UniProtKB: Sm protein F

Macromolecule #52: Sm protein G

• Macromolecule: Name: Sm protein G / type: protein_or_peptide / ID: 52 / Number of copies: 3 / Enantiomer: LEVO
• Source (natural): Organism: Leishmania tarentolae (eukaryote)
• Molecular weight: Theoretical: 8.810194 KDa

Sequence

String:

MPPHKTLPNL NHFMEKRIVV KIQGGRSISG VLRGVDEHMS IVLHEAIDET RNAAANEEPK MALGTTVIRG SAIVEIVSAD V

UniProtKB: Sm protein G

Macromolecule #53: Small nuclear ribonucleoprotein Sm D3

• Macromolecule: Name: Small nuclear ribonucleoprotein Sm D3 / type: protein_or_peptide / ID: 53 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: Leishmania tarentolae (eukaryote)
• Molecular weight: Theoretical: 12.345234 KDa

Sequence

String:

MAESIPIKVL FDALHLKVSI EVATGEVYHG TLEDLQNNMN VLLKDAIKIV RGGKETRMES VFVRGSNIVF FQLPDALQTS PALLRAGEV VSKAKDTRGD GKGFGASRKR ARNS

UniProtKB: Small nuclear ribonucleoprotein Sm D3

Macromolecule #54: Sm protein B

• Macromolecule: Name: Sm protein B / type: protein_or_peptide / ID: 54 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: Leishmania tarentolae (eukaryote)
• Molecular weight: Theoretical: 11.87281 KDa

Sequence

String:

MGKYTMLHNI NKVLCVVLDD GRNITGKLLV FDKHMNVVLG DAVEERPQSK KMAEEGVSSK RQLGLILLRG EHVVSVTVMK DGESGGSGA AANFSSAPKS SKATAVKRKR GA

UniProtKB: Sm protein B

Macromolecule #45: Y-branched pre-mRNA

• Macromolecule: Name: Y-branched pre-mRNA / type: rna / ID: 45 / Number of copies: 1
• Source (natural): Organism: Leishmania tarentolae (eukaryote)
• Molecular weight: Theoretical: 13.70483 KDa

Sequence

String:

A(N)(N)(N)(N)(N)(N)(N)(N)(N) (N)(N)(N)(N)(N)(N)(N)(N)(N)(N) (N)(N)(N)(N)(N)(N)(N) (N)(N) (N)(N)(N)(N)(N)(N)(N)(N)(N)(N) (N)(N)(N)AG(N)(N)(N)(N)(N) (N)(N)(N)(N)(N)(N) (N)(N)(N) (N)(N)(N)(N)(N)(N)(N)(N)(N)(N)

Macromolecule #55: SL RNA exon

• Macromolecule: Name: SL RNA exon / type: rna / ID: 55 / Number of copies: 1
• Source (natural): Organism: Leishmania tarentolae (eukaryote)
• Molecular weight: Theoretical: 12.361283 KDa

Sequence

String:

AACUAACGCU AUAUAAGUAU CAGUUUC(PSU)GU ACUUUAUUG

Macromolecule #56: SL RNA outron

• Macromolecule: Name: SL RNA outron / type: rna / ID: 56 / Number of copies: 1
• Source (natural): Organism: Leishmania tarentolae (eukaryote)
• Molecular weight: Theoretical: 18.331889 KDa

Sequence

String:

GUAUGCGAAA CUUCCGGAAC CUGUCUUCCG GCAAGAUUUU GGAAGCGCGC AGGCGCU

GENBANK: GENBANK: X73121.1

Macromolecule #57: U2 snRNA

• Macromolecule: Name: U2 snRNA / type: rna / ID: 57 / Number of copies: 1
• Source (natural): Organism: Leishmania tarentolae (eukaryote)
• Molecular weight: Theoretical: 45.393723 KDa

Sequence

String:

AUAUCUUCUC GGCUAUUUAG CUAAGAUCAU GUUUAUAAAC UGUUCUUAUC AGAGUAACUC CUGAUACUGC CUUCGGGCAA AGGAAUAGA AAUUAUAACU CAAGGUUGUU UCCCUGGAGU UCCACGUUUC CAGGGGAGCA ACU

GENBANK: GENBANK: AY007788.1

Macromolecule #58: U5 snRNA

• Macromolecule: Name: U5 snRNA / type: rna / ID: 58 / Number of copies: 1
• Source (natural): Organism: Leishmania tarentolae (eukaryote)
• Molecular weight: Theoretical: 22.591439 KDa

Sequence

String:

AACCGCUGCU UCGACUUUUA CUAAGCAGCG CUCCCUACCC UACGCACCAA UUUUGAGGCC CAAAAGGGCA U

GENBANK: GENBANK: CP119860.1

Macromolecule #59: U6 snRNA

• Macromolecule: Name: U6 snRNA / type: rna / ID: 59 / Number of copies: 1
• Source (natural): Organism: Leishmania tarentolae (eukaryote)
• Molecular weight: Theoretical: 32.113098 KDa

Sequence

String:

GAGCCCCUUC GGGGGACAUC CACAAACUGG AAAUUCAACA GAGAAGAUUA GCACUCUCCC UGCGCAAGGC UGAUGUCAAU CUUCGAGAG AUAUAGCUUU U

GENBANK: GENBANK: AF305715.1

Macromolecule #60: MAGNESIUM ION

• Macromolecule: Name: MAGNESIUM ION / type: ligand / ID: 60 / Number of copies: 12 / Formula: MG
• Molecular weight: Theoretical: 24.305 Da

Macromolecule #61: ADENOSINE-5'-DIPHOSPHATE

• Macromolecule: Name: ADENOSINE-5'-DIPHOSPHATE / type: ligand / ID: 61 / Number of copies: 1 / Formula: ADP
• Molecular weight: Theoretical: 427.201 Da

Chemical component information

ChemComp-ADP:
ADENOSINE-5'-DIPHOSPHATE / ADP, energy-carrying molecule*YM

Macromolecule #62: POTASSIUM ION

• Macromolecule: Name: POTASSIUM ION / type: ligand / ID: 62 / Number of copies: 1 / Formula: K
• Molecular weight: Theoretical: 39.098 Da

Macromolecule #63: INOSITOL HEXAKISPHOSPHATE

• Macromolecule: Name: INOSITOL HEXAKISPHOSPHATE / type: ligand / ID: 63 / Number of copies: 1 / Formula: IHP
• Molecular weight: Theoretical: 660.035 Da

Chemical component information

ChemComp-IHP:
INOSITOL HEXAKISPHOSPHATE

Macromolecule #64: GUANOSINE-5'-TRIPHOSPHATE

• Macromolecule: Name: GUANOSINE-5'-TRIPHOSPHATE / type: ligand / ID: 64 / Number of copies: 1 / Formula: GTP
• Molecular weight: Theoretical: 523.18 Da

Chemical component information

ChemComp-GTP:
GUANOSINE-5'-TRIPHOSPHATE / GTP, energy-carrying molecule*YM

Macromolecule #65: ZINC ION

• Macromolecule: Name: ZINC ION / type: ligand / ID: 65 / Number of copies: 5 / Formula: ZN
• Molecular weight: Theoretical: 65.409 Da

Macromolecule #66: 7-METHYL-GUANOSINE-5'-TRIPHOSPHATE-5'-GUANOSINE

• Macromolecule: Name: 7-METHYL-GUANOSINE-5'-TRIPHOSPHATE-5'-GUANOSINE / type: ligand / ID: 66 / Number of copies: 1 / Formula: GTG
• Molecular weight: Theoretical: 803.44 Da

Chemical component information

ChemComp-GTG:
7-METHYL-GUANOSINE-5'-TRIPHOSPHATE-5'-GUANOSINE

Macromolecule #67: 5'-O-[(S)-hydroxy{[(R)-hydroxy{[(S)-hydroxy(methoxy)phosphoryl]ox...

• Macromolecule: Name: 5'-O-[(S)-hydroxy{[(R)-hydroxy{[(S)-hydroxy(methoxy)phosphoryl]oxy}phosphoryl]oxy}phosphoryl]guanosine; type: ligand / ID: 67 / Number of copies: 1 / Formula: G5J
• Molecular weight: Theoretical: 537.207 Da

Chemical component information

ChemComp-G5J:
5'-O-[(S)-hydroxy{[(R)-hydroxy{[(S)-hydroxy(methoxy)phosphoryl]oxy}phosphoryl]oxy}phosphoryl]guanosine

Experimental details

Structure determination

• Method: cryo EM
• Processing: single particle reconstruction
• Aggregation state: particle

Sample preparation

• Buffer: pH: 7.8
• Grid: Model: Quantifoil R3.5/1 / Material: GOLD / Support film - Material: CARBON / Support film - Film thickness: 2 / Pretreatment - Type: GLOW DISCHARGE / Pretreatment - Time: 30 sec.
• Vitrification: Cryogen name: ETHANE / Chamber humidity: 100 % / Chamber temperature: 283 K / Instrument: FEI VITROBOT MARK IV

Electron microscopy

• Microscope: TFS KRIOS
• Specialist optics: Energy filter - Slit width: 20 eV
• Image recording: Film or detector model: GATAN K3 BIOQUANTUM (6k x 4k) / Number grids imaged: 2 / Number real images: 31410 / Average exposure time: 2.0 sec. / Average electron dose: 50.0 e/Ų
• Electron beam: Acceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
• Electron optics: Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Nominal defocus max: 2.5 µm / Nominal defocus min: 0.5 µm / Nominal magnification: 64000
• Experimental equipment: Model: Titan Krios / Image courtesy: FEI Company

Image processing

• Particle selection: Number selected: 1214398
• CTF correction: Software - Name: cryoSPARC (ver. 4.6.0) / Type: PHASE FLIPPING AND AMPLITUDE CORRECTION
• Startup model: Type of model: OTHER / Details: ab initio model
• Final reconstruction: Resolution.type: BY AUTHOR / Resolution: 2.7 Å / Resolution method: FSC 0.143 CUT-OFF / Software - Name: cryoSPARC (ver. 4.6.0) / Number images used: 325589
• Initial angle assignment: Type: OTHER / Software - Name: cryoSPARC (ver. 4.6.0)
• Final angle assignment: Type: OTHER / Software - Name: cryoSPARC (ver. 4.6.0)
• Final 3D classification: Software: (Name: cryoSPARC (ver. 4.6.0), RELION (ver. 5.0))