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Yorodumi- EMDB-43914: Subtomogram average of the HN-F complex on the viral surface of a... -
+Open data
-Basic information
Entry | Database: EMDB / ID: EMD-43914 | ||||||||||||||||||
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Title | Subtomogram average of the HN-F complex on the viral surface of an HPIV3 escape variant (EV1). | ||||||||||||||||||
Map data | Subtomogram average of the HN-F complex on the viral surface of an HPIV3 escape variant (EV1) | ||||||||||||||||||
Sample |
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Keywords | virus / glycoproteins / fusion protein / Hemagglutinin-neuraminidase / viral evolution / tomography / fusion complex / HPIV3 / paraflu / VIRAL PROTEIN | ||||||||||||||||||
Biological species | Human respirovirus 3 | ||||||||||||||||||
Method | subtomogram averaging / cryo EM / Resolution: 12.2 Å | ||||||||||||||||||
Authors | Marcink TC / Moscona A | ||||||||||||||||||
Funding support | United States, 5 items
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Citation | Journal: To Be Published Title: Hats off: How a Paramyxovirus fusion/entry complex adapts to escape a neutralizing antibody. Authors: Marcink TC / Moscona A | ||||||||||||||||||
History |
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-Structure visualization
Supplemental images |
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-Downloads & links
-EMDB archive
Map data | emd_43914.map.gz | 5.9 MB | EMDB map data format | |
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Header (meta data) | emd-43914-v30.xml emd-43914.xml | 13.9 KB 13.9 KB | Display Display | EMDB header |
FSC (resolution estimation) | emd_43914_fsc.xml | 7.4 KB | Display | FSC data file |
Images | emd_43914.png | 46.5 KB | ||
Filedesc metadata | emd-43914.cif.gz | 4.2 KB | ||
Others | emd_43914_half_map_1.map.gz emd_43914_half_map_2.map.gz | 6 MB 6 MB | ||
Archive directory | http://ftp.pdbj.org/pub/emdb/structures/EMD-43914 ftp://ftp.pdbj.org/pub/emdb/structures/EMD-43914 | HTTPS FTP |
-Validation report
Summary document | emd_43914_validation.pdf.gz | 886.6 KB | Display | EMDB validaton report |
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Full document | emd_43914_full_validation.pdf.gz | 886.2 KB | Display | |
Data in XML | emd_43914_validation.xml.gz | 11.6 KB | Display | |
Data in CIF | emd_43914_validation.cif.gz | 15.5 KB | Display | |
Arichive directory | https://ftp.pdbj.org/pub/emdb/validation_reports/EMD-43914 ftp://ftp.pdbj.org/pub/emdb/validation_reports/EMD-43914 | HTTPS FTP |
-Related structure data
-Links
EMDB pages | EMDB (EBI/PDBe) / EMDataResource |
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-Map
File | Download / File: emd_43914.map.gz / Format: CCP4 / Size: 8 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) | ||||||||||||||||||||||||||||||||||||
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Annotation | Subtomogram average of the HN-F complex on the viral surface of an HPIV3 escape variant (EV1) | ||||||||||||||||||||||||||||||||||||
Projections & slices | Image control
Images are generated by Spider. | ||||||||||||||||||||||||||||||||||||
Voxel size | X=Y=Z: 3.422 Å | ||||||||||||||||||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||||||||||||||||||
Details | EMDB XML:
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-Supplemental data
-Sample components
-Entire : Human respirovirus 3
Entire | Name: Human respirovirus 3 |
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Components |
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-Supramolecule #1: Human respirovirus 3
Supramolecule | Name: Human respirovirus 3 / type: virus / ID: 1 / Parent: 0 / NCBI-ID: 11216 / Sci species name: Human respirovirus 3 / Virus type: VIRION / Virus isolate: STRAIN / Virus enveloped: Yes / Virus empty: No |
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-Experimental details
-Structure determination
Method | cryo EM |
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Processing | subtomogram averaging |
Aggregation state | cell |
-Sample preparation
Buffer | pH: 7.4 |
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Grid | Model: PELCO Ultrathin Carbon with Lacey Carbon / Material: GOLD / Mesh: 300 / Support film - Material: CARBON / Support film - topology: LACEY / Support film - Film thickness: 5 |
Vitrification | Cryogen name: ETHANE / Chamber humidity: 100 % / Chamber temperature: 277.15 K |
-Electron microscopy
Microscope | FEI TITAN |
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Image recording | Film or detector model: GATAN K3 (6k x 4k) / Average electron dose: 3.0 e/Å2 |
Electron beam | Acceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN |
Electron optics | Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Nominal defocus max: 3.5 µm / Nominal defocus min: 3.5 µm |