National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)
R35GM142553
United States
National Institutes of Health/National Institute of Diabetes and Digestive and Kidney Disease (NIH/NIDDK)
R01DK125263
United States
National Institutes of Health/National Cancer Institute (NIH/NCI)
R37CA248565
United States
Jane Coffin Childs (JCC) Fund
United States
Swiss National Science Foundation
P2BSP3_188112
Switzerland
Swiss National Science Foundation
P400PB_199252
Switzerland
Citation
Journal: EMBO J / Year: 2024 Title: In situ architecture of Opa1-dependent mitochondrial cristae remodeling. Authors: Michelle Y Fry / Paula P Navarro / Pusparanee Hakim / Virly Y Ananda / Xingping Qin / Juan C Landoni / Sneha Rath / Zintis Inde / Camila Makhlouta Lugo / Bridget E Luce / Yifan Ge / Julie L ...Authors: Michelle Y Fry / Paula P Navarro / Pusparanee Hakim / Virly Y Ananda / Xingping Qin / Juan C Landoni / Sneha Rath / Zintis Inde / Camila Makhlouta Lugo / Bridget E Luce / Yifan Ge / Julie L McDonald / Ilzat Ali / Leillani L Ha / Benjamin P Kleinstiver / David C Chan / Kristopher A Sarosiek / Luke H Chao / Abstract: Cristae membrane state plays a central role in regulating mitochondrial function and cellular metabolism. The protein Optic atrophy 1 (Opa1) is an important crista remodeler that exists as two forms ...Cristae membrane state plays a central role in regulating mitochondrial function and cellular metabolism. The protein Optic atrophy 1 (Opa1) is an important crista remodeler that exists as two forms in the mitochondrion, a membrane-anchored long form (l-Opa1) and a processed short form (s-Opa1). The mechanisms for how Opa1 influences cristae shape have remained unclear due to lack of native three-dimensional views of cristae. We perform in situ cryo-electron tomography of cryo-focused ion beam milled mouse embryonic fibroblasts with defined Opa1 states to understand how each form of Opa1 influences cristae architecture. In our tomograms, we observe a variety of cristae shapes with distinct trends dependent on s-Opa1:l-Opa1 balance. Increased l-Opa1 levels promote cristae stacking and elongated mitochondria, while increased s-Opa1 levels correlated with irregular cristae packing and round mitochondria shape. Functional assays indicate a role for l-Opa1 in wild-type apoptotic and calcium handling responses, and show a compromised respiratory function under Opa1 imbalance. In summary, we provide three-dimensional visualization of cristae architecture to reveal relationships between mitochondrial ultrastructure and cellular function dependent on Opa1-mediated membrane remodeling.
Download / File: emd_43051.map.gz / Format: CCP4 / Size: 280.7 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Voxel size
X=Y=Z: 22.06 Å
Density
Minimum - Maximum
-34.659660000000002 - 80.008020000000002
Average (Standard dev.)
13.993497 (±2.6963806)
Symmetry
Space group: 1
Details
EMDB XML:
Map geometry
Axis order
X
Y
Z
Origin
-104
104
-100
Dimensions
720
511
200
Spacing
511
720
200
Cell
A: 11272.66 Å / B: 15883.199 Å / C: 4412.0 Å α=β=γ: 90.0 °
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Supplemental data
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Sample components
-
Entire : mitochondria in mouse embryonic fibroblasts with OMA1 deletion an...
Entire
Name: mitochondria in mouse embryonic fibroblasts with OMA1 deletion and OPA1-exon5b deletion
Components
Cell: mitochondria in mouse embryonic fibroblasts with OMA1 deletion and OPA1-exon5b deletion
-
Supramolecule #1: mitochondria in mouse embryonic fibroblasts with OMA1 deletion an...
Supramolecule
Name: mitochondria in mouse embryonic fibroblasts with OMA1 deletion and OPA1-exon5b deletion type: cell / ID: 1 / Parent: 0
Source (natural)
Organism: Mus musculus (house mouse)
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Experimental details
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Structure determination
Method
cryo EM
Processing
electron tomography
Aggregation state
cell
-
Sample preparation
Buffer
pH: 7.5
Grid
Model: Quantifoil R2/2 / Material: GOLD / Mesh: 200 / Support film - Material: CARBON / Support film - topology: HOLEY
Vitrification
Cryogen name: ETHANE / Chamber humidity: 100 % / Chamber temperature: 277.15 K / Instrument: FEI VITROBOT MARK IV
Sectioning
Focused ion beam - Instrument: OTHER / Focused ion beam - Ion: OTHER / Focused ion beam - Voltage: 5 / Focused ion beam - Current: 0.01 / Focused ion beam - Duration: 600 / Focused ion beam - Temperature: 88 K / Focused ion beam - Initial thickness: 1000 / Focused ion beam - Final thickness: 200 Focused ion beam - Details: The value given for _em_focused_ion_beam.instrument is TFS Aquilos2. This is not in a list of allowed values {'DB235', 'OTHER'} so OTHER is written into the XML file.
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Electron microscopy
Microscope
FEI TITAN KRIOS
Image recording
Film or detector model: GATAN K3 BIOQUANTUM (6k x 4k) / Average electron dose: 2.81 e/Å2
Electron beam
Acceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
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