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- EMDB-29761: Cryo-Electron Tomography of a Cryo-Lamella Milled Inside a Zebra ... -
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Open data
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Basic information
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Title | Cryo-Electron Tomography of a Cryo-Lamella Milled Inside a Zebra Fish Muscle Tissue Biopsy | ||||||||||||
![]() | Cryo-Electron Tomography of Zebra Fish Muscle Tissue Biopsy | ||||||||||||
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![]() | Thin filament / thick filament / MOTOR PROTEIN | ||||||||||||
Biological species | ![]() ![]() | ||||||||||||
Method | electron tomography | ||||||||||||
![]() | Trepout S / Ramm G | ||||||||||||
Funding support | ![]() ![]()
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![]() | ![]() Title: Cryo-Electron Tomography of a Cryo-Lamella Milled Inside a Zebra Fish Muscle Tissue Biopsy Authors: Trepout S / Ramm G | ||||||||||||
History |
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Structure visualization
Supplemental images |
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Downloads & links
-EMDB archive
Map data | ![]() | 3.7 GB | ![]() | |
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Header (meta data) | ![]() ![]() | 9 KB 9 KB | Display Display | ![]() |
Images | ![]() | 250.3 KB | ||
Filedesc metadata | ![]() | 3.6 KB | ||
Archive directory | ![]() ![]() | HTTPS FTP |
-Validation report
Summary document | ![]() | 386.8 KB | Display | ![]() |
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Full document | ![]() | 386.4 KB | Display | |
Data in XML | ![]() | 5.1 KB | Display | |
Data in CIF | ![]() | 5.6 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
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Links
EMDB pages | ![]() ![]() |
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Map
File | ![]() | ||||||||||||||||||||
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Annotation | Cryo-Electron Tomography of Zebra Fish Muscle Tissue Biopsy | ||||||||||||||||||||
Voxel size | X=Y=Z: 14.66 Å | ||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||
Details | EMDB XML:
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-Supplemental data
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Sample components
-Entire : Biopsy of Zebra Fish Muscle
Entire | Name: Biopsy of Zebra Fish Muscle |
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Components |
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-Supramolecule #1: Biopsy of Zebra Fish Muscle
Supramolecule | Name: Biopsy of Zebra Fish Muscle / type: tissue / ID: 1 / Parent: 0 Details: Cryo-lamella cut inside the biopsy using the bbq method. |
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Source (natural) | Organism: ![]() ![]() |
-Experimental details
-Structure determination
![]() | electron tomography |
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Aggregation state | tissue |
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Sample preparation
Buffer | pH: 6.9 |
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High pressure freezing | Instrument: OTHER Details: The value given for _em_high_pressure_freezing.instrument is HPF Wohlwend Compact 03. This is not in a list of allowed values {'EMS-002 RAPID IMMERSION FREEZER', 'BAL-TEC HPM 010', 'LEICA EM ...Details: The value given for _em_high_pressure_freezing.instrument is HPF Wohlwend Compact 03. This is not in a list of allowed values {'EMS-002 RAPID IMMERSION FREEZER', 'BAL-TEC HPM 010', 'LEICA EM PACT2', 'OTHER', 'LEICA EM PACT', 'LEICA EM HPM100'} so OTHER is written into the XML file. |
Sectioning | Focused ion beam - Instrument: OTHER / Focused ion beam - Ion: OTHER / Focused ion beam - Voltage: 30 / Focused ion beam - Current: 1 / Focused ion beam - Duration: 1800 / Focused ion beam - Temperature: 100 K / Focused ion beam - Initial thickness: 100000 / Focused ion beam - Final thickness: 250 Focused ion beam - Details: The value given for _em_focused_ion_beam.instrument is FEI Helios G4 UX. This is not in a list of allowed values {'DB235', 'OTHER'} so OTHER is written into the XML file. |
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Electron microscopy
Microscope | FEI TITAN KRIOS |
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Specialist optics | Energy filter - Name: GIF Bioquantum / Energy filter - Slit width: 20 eV |
Image recording | Film or detector model: GATAN K2 QUANTUM (4k x 4k) / Detector mode: COUNTING / Average electron dose: 1.5 e/Å2 |
Electron beam | Acceleration voltage: 300 kV / Electron source: ![]() |
Electron optics | Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Nominal defocus max: 4.0 µm / Nominal defocus min: 4.0 µm / Nominal magnification: 19500 |
Sample stage | Specimen holder model: FEI TITAN KRIOS AUTOGRID HOLDER / Cooling holder cryogen: NITROGEN |
Experimental equipment | ![]() Model: Titan Krios / Image courtesy: FEI Company |
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Image processing
Final reconstruction | Algorithm: BACK PROJECTION / Software - Name: ![]() |
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