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Open data
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Basic information
Entry | ![]() | ||||||||||||||||||
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Title | Reconstituted chromatin condensed by the PRC1-CBX8 complex | ||||||||||||||||||
![]() | Cryo-tomogram of reconstituted chromatin with a repressive modifier | ||||||||||||||||||
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![]() | Chromatin structure / Polycomb / transcriptional regulation / repressive chromatin modifier / GENE REGULATION | ||||||||||||||||||
Biological species | ![]() | ||||||||||||||||||
Method | electron tomography / cryo EM | ||||||||||||||||||
![]() | Uckelmann M / Taveneau C / Levina V / de Marco A / Davidovich C | ||||||||||||||||||
Funding support | ![]()
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![]() | ![]() Title: The three-dimensional structure of chromatin condensed by PRC1 is porous and accessible Authors: Uckelmann M / Levina V / Taveneau C / Ng XH / Pandey V / Zhang Q / Li M / Das PP / de Marco A / Davidovich C | ||||||||||||||||||
History |
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Structure visualization
Supplemental images |
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Downloads & links
-EMDB archive
Map data | ![]() | 674.7 MB | ![]() | |
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Header (meta data) | ![]() ![]() | 11.1 KB 11.1 KB | Display Display | ![]() |
Images | ![]() | 63.1 KB | ||
Filedesc metadata | ![]() | 4.3 KB | ||
Archive directory | ![]() ![]() | HTTPS FTP |
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Links
EMDB pages | ![]() ![]() |
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Map
File | ![]() | ||||||||||||||||||||
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Annotation | Cryo-tomogram of reconstituted chromatin with a repressive modifier | ||||||||||||||||||||
Voxel size | X=Y=Z: 5.28 Å | ||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||
Details | EMDB XML:
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-Supplemental data
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Sample components
-Entire : Reconstituted chromatin condensed by PRC1-CBX8
Entire | Name: Reconstituted chromatin condensed by PRC1-CBX8 |
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Components |
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-Supramolecule #1: Reconstituted chromatin condensed by PRC1-CBX8
Supramolecule | Name: Reconstituted chromatin condensed by PRC1-CBX8 / type: complex / ID: 1 / Parent: 0 |
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Source (natural) | Organism: ![]() |
-Supramolecule #2: PRC1-CBX8
Supramolecule | Name: PRC1-CBX8 / type: complex / ID: 2 / Parent: 1 Details: Three-protein complex of RING1b, BMI1, and CBX8-MBP |
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Source (natural) | Organism: ![]() |
-Experimental details
-Structure determination
Method | cryo EM |
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![]() | electron tomography |
Aggregation state | 3D array |
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Sample preparation
Buffer | pH: 7.5 Component:
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Grid | Model: Quantifoil R1.2/1.3 / Material: COPPER / Mesh: 200 / Pretreatment - Type: GLOW DISCHARGE / Pretreatment - Time: 30 sec. / Pretreatment - Atmosphere: AIR / Details: 30 seconds, 25 mAmp, 0.24 mBar | ||||||||||||||||||
Vitrification | Cryogen name: ETHANE / Chamber humidity: 100 % / Chamber temperature: 277.15 K / Instrument: FEI VITROBOT MARK IV | ||||||||||||||||||
Sectioning | Other: NO SECTIONING | ||||||||||||||||||
Fiducial marker | Diameter: 5 nm |
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Electron microscopy
Microscope | FEI TITAN KRIOS |
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Image recording | Film or detector model: GATAN K2 SUMMIT (4k x 4k) / Number real images: 41 / Average electron dose: 3.52 e/Å2 |
Electron beam | Acceleration voltage: 300 kV / Electron source: ![]() |
Electron optics | Illumination mode: SPOT SCAN / Imaging mode: BRIGHT FIELD / Cs: 2.7 mm / Nominal defocus max: 3.5 µm / Nominal defocus min: 3.5 µm |
Experimental equipment | ![]() Model: Titan Krios / Image courtesy: FEI Company |
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Image processing
Final reconstruction | Algorithm: BACK PROJECTION / Software - Name: ![]() |
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