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Open data
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Basic information
Entry | ![]() | |||||||||
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Title | Q. thermotolerans Engineered Encapsulin P3P4 | |||||||||
![]() | Sharp map from CryoSPARC refinement. | |||||||||
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![]() | Encapsulin / Nanocage / Compartment / Storage / Complex / VIRUS LIKE PARTICLE | |||||||||
Function / homology | Type 1 encapsulin shell protein / Encapsulating protein for peroxidase / encapsulin nanocompartment / iron ion transport / intracellular iron ion homeostasis / Type 1 encapsulin shell protein![]() | |||||||||
Biological species | ![]() ![]() | |||||||||
Method | single particle reconstruction / cryo EM / Resolution: 3.1 Å | |||||||||
![]() | Satler T / Jerala R | |||||||||
Funding support | ![]()
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![]() | ![]() Title: Structure of engineered encapsulin P3P4 Authors: Satler T / Jerala R | |||||||||
History |
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Structure visualization
Supplemental images |
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Downloads & links
-EMDB archive
Map data | ![]() | 567.6 MB | ![]() | |
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Header (meta data) | ![]() ![]() | 14.8 KB 14.8 KB | Display Display | ![]() |
FSC (resolution estimation) | ![]() | 17.8 KB | Display | ![]() |
Images | ![]() | 283 KB | ||
Masks | ![]() | 600.7 MB | ![]() | |
Filedesc metadata | ![]() | 4.1 KB | ||
Others | ![]() ![]() ![]() | 300.8 MB 557.8 MB 557.8 MB | ||
Archive directory | ![]() ![]() | HTTPS FTP |
-Validation report
Summary document | ![]() | 1.2 MB | Display | ![]() |
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Full document | ![]() | 1.2 MB | Display | |
Data in XML | ![]() | 27.6 KB | Display | |
Data in CIF | ![]() | 36.3 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 8rvjMC M: atomic model generated by this map C: citing same article ( |
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Similar structure data | Similarity search - Function & homology ![]() |
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Links
EMDB pages | ![]() ![]() |
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Related items in Molecule of the Month |
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Map
File | ![]() | ||||||||||||||||||||
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Annotation | Sharp map from CryoSPARC refinement. | ||||||||||||||||||||
Voxel size | X=Y=Z: 1.2 Å | ||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||
Details | EMDB XML:
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-Supplemental data
-Mask #1
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Projections & Slices |
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Density Histograms |
-Additional map: Basic map from CryoSPARC refinement.
File | emd_19521_additional_1.map | ||||||||||||
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Annotation | Basic map from CryoSPARC refinement. | ||||||||||||
Projections & Slices |
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Density Histograms |
-Half map: Half map 1 from CryoSPARC reinement.
File | emd_19521_half_map_1.map | ||||||||||||
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Annotation | Half map 1 from CryoSPARC reinement. | ||||||||||||
Projections & Slices |
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Density Histograms |
-Half map: Half map 2 from CryoSPARC reinement.
File | emd_19521_half_map_2.map | ||||||||||||
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Annotation | Half map 2 from CryoSPARC reinement. | ||||||||||||
Projections & Slices |
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Density Histograms |
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Sample components
-Entire : Engineered Encapsulin P3P4
Entire | Name: Engineered Encapsulin P3P4 |
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Components |
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-Supramolecule #1: Engineered Encapsulin P3P4
Supramolecule | Name: Engineered Encapsulin P3P4 / type: complex / ID: 1 / Parent: 0 |
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Source (natural) | Organism: ![]() ![]() |
-Experimental details
-Structure determination
Method | cryo EM |
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![]() | single particle reconstruction |
Aggregation state | particle |
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Sample preparation
Concentration | 0.8 mg/mL | |||||||||
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Buffer | pH: 7.5 Component:
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Grid | Model: Quantifoil R1.2/1.3 / Material: COPPER / Mesh: 300 / Support film - Material: CARBON / Support film - topology: HOLEY ARRAY / Pretreatment - Type: GLOW DISCHARGE | |||||||||
Vitrification | Cryogen name: ETHANE / Chamber humidity: 95 % / Chamber temperature: 277.15 K |
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Electron microscopy
Microscope | TFS GLACIOS |
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Image recording | Film or detector model: FEI FALCON III (4k x 4k) / Detector mode: COUNTING / Average electron dose: 40.0 e/Å2 |
Electron beam | Acceleration voltage: 200 kV / Electron source: ![]() |
Electron optics | Illumination mode: OTHER / Imaging mode: BRIGHT FIELD / Nominal defocus max: 2.9 µm / Nominal defocus min: 0.9 µm |