ジャーナル: Elife / 年: 2020 タイトル: Talin-activated vinculin interacts with branched actin networks to initiate bundles. 著者: Rajaa Boujemaa-Paterski / Bruno Martins / Matthias Eibauer / Charlie T Beales / Benjamin Geiger / Ohad Medalia / 要旨: Vinculin plays a fundamental role in integrin-mediated cell adhesion. Activated by talin, it interacts with diverse adhesome components, enabling mechanical coupling between the actin cytoskeleton ...Vinculin plays a fundamental role in integrin-mediated cell adhesion. Activated by talin, it interacts with diverse adhesome components, enabling mechanical coupling between the actin cytoskeleton and the extracellular matrix. Here we studied the interactions of activated full-length vinculin with actin and the way it regulates the organization and dynamics of the Arp2/3 complex-mediated branched actin network. Through a combination of surface patterning and light microscopy experiments we show that vinculin can bundle dendritic actin networks through rapid binding and filament crosslinking. We show that vinculin promotes stable but flexible actin bundles having a mixed-polarity organization, as confirmed by cryo-electron tomography. Adhesion-like synthetic design of vinculin activation by surface-bound talin revealed that clustered vinculin can initiate and immobilize bundles from mobile Arp2/3-branched networks. Our results provide a molecular basis for coordinate actin bundle formation at nascent adhesions.
EMPIAR-10548 (タイトル: Cryo-ET of actin bundles induced by full length vinculin Data size: 4.0 Data #1: Tomograms of actin bundles induced by full length vinculin [tilt series])