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- PDB-8ita: A reversible glycosyltransferase of tectorigenin - Bc7OUGT comple... -
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Open data
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Basic information
Entry | Database: PDB / ID: 8ita | ||||||
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Title | A reversible glycosyltransferase of tectorigenin - Bc7OUGT complexed with UDP and tectorigenin | ||||||
![]() | Bc7OUGT | ||||||
![]() | TRANSFERASE / glycosyltransferase / UGT / GT-B fold structure / Rossmann-like domain. | ||||||
Function / homology | Chem-R0U / URIDINE-5'-DIPHOSPHATE![]() | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Zhang, Z.Y. / Lu, L. / Guan, Z.F. / Cheng, W.J. | ||||||
Funding support | ![]()
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![]() | ![]() Title: Functional characterization and structural basis of a reversible glycosyltransferase involves in plant chemical defence. Authors: Cheng, W. / Fang, X. / Guan, Z. / Yao, Y. / Xu, Z. / Bi, Y. / Ren, K. / Li, J. / Chen, F. / Chen, X. / Ma, W. / Chu, Z. / Deng, Z. / Zhang, Z. / Lu, L. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 100.8 KB | Display | ![]() |
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PDB format | ![]() | 73.5 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 1 MB | Display | ![]() |
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Full document | ![]() | 1 MB | Display | |
Data in XML | ![]() | 18.1 KB | Display | |
Data in CIF | ![]() | 24.7 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 8inpC C: citing same article ( |
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Similar structure data | Similarity search - Function & homology ![]() |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 51691.012 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() |
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#2: Chemical | ChemComp-R0U / |
#3: Chemical | ChemComp-UDP / |
#4: Water | ChemComp-HOH / |
Has ligand of interest | Y |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 3.16 Å3/Da / Density % sol: 61.16 % |
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, sitting drop / pH: 8.5 Details: 0.17M Lithium sulfate monohydrate, 0.085M Tris hydrochloride pH8.5, 25.5% w/v PEG 4000 |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: DECTRIS PILATUS3 6M / Detector: PIXEL / Date: Nov 25, 2022 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.9791 Å / Relative weight: 1 |
Reflection | Resolution: 2.5→24.84 Å / Num. obs: 18983 / % possible obs: 82 % / Redundancy: 3 % / Rmerge(I) obs: 0.086 / Net I/σ(I): 8 |
Reflection shell | Resolution: 2.5→2.6 Å / Rmerge(I) obs: 0.865 / Num. unique obs: 2219 |
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Processing
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Refinement | Method to determine structure: ![]()
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.5→24.84 Å
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Refine LS restraints |
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LS refinement shell |
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