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- PDB-7ncg: Type 2A alpha-synuclein filament seeded in vitro by filaments pur... -
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Open data
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Basic information
Entry | Database: PDB / ID: 7ncg | |||||||||||||||
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Title | Type 2A alpha-synuclein filament seeded in vitro by filaments purified from Multiple Systems Atrophy Case 2 | |||||||||||||||
![]() | Alpha-synuclein![]() | |||||||||||||||
![]() | PROTEIN FIBRIL / ![]() ![]() ![]() ![]() ![]() | |||||||||||||||
Function / homology | ![]() negative regulation of mitochondrial electron transport, NADH to ubiquinone / neutral lipid metabolic process / ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() Similarity search - Function | |||||||||||||||
Biological species | ![]() ![]() | |||||||||||||||
Method | ![]() ![]() | |||||||||||||||
![]() | Lovestam, S.K.A. / Schweighauser, M. / Scheres, S.H.W. | |||||||||||||||
Funding support | ![]() ![]()
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![]() | ![]() Title: Seeded assembly in vitro does not replicate the structures of α-synuclein filaments from multiple system atrophy. Authors: Sofia Lövestam / Manuel Schweighauser / Tomoyasu Matsubara / Shigeo Murayama / Taisuke Tomita / Takashi Ando / Kazuko Hasegawa / Mari Yoshida / Airi Tarutani / Masato Hasegawa / Michel ...Authors: Sofia Lövestam / Manuel Schweighauser / Tomoyasu Matsubara / Shigeo Murayama / Taisuke Tomita / Takashi Ando / Kazuko Hasegawa / Mari Yoshida / Airi Tarutani / Masato Hasegawa / Michel Goedert / Sjors H W Scheres / ![]() ![]() Abstract: The propagation of conformational strains by templated seeding is central to the prion concept. Seeded assembly of α-synuclein into filaments is believed to underlie the prion-like spreading of ...The propagation of conformational strains by templated seeding is central to the prion concept. Seeded assembly of α-synuclein into filaments is believed to underlie the prion-like spreading of protein inclusions in a number of human neurodegenerative diseases, including Parkinson's disease, dementia with Lewy bodies (DLB) and multiple system atrophy (MSA). We previously determined the atomic structures of α-synuclein filaments from the putamen of five individuals with MSA. Here, we used filament preparations from three of these brains for the in vitro seeded assembly of recombinant human α-synuclein. We find that the structures of the seeded assemblies differ from those of the seeds, suggesting that additional, as yet unknown, factors play a role in the propagation of the seeds. Identification of these factors will be essential for understanding the prion-like spreading of α-synuclein proteinopathies. | |||||||||||||||
History |
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Structure visualization
Movie |
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Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 227.3 KB | Display | ![]() |
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PDB format | ![]() | 184 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Related structure data | ![]() 12265MC ![]() 7ncaC ![]() 7nchC ![]() 7nciC ![]() 7ncjC ![]() 7nckC M: map data used to model this data C: citing same article ( |
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Similar structure data | |
EM raw data | ![]() Data size: 1.1 TB Data #1: Unaligned movies of alpha-synuclein filament seeded in vitro by filaments purified from Multiple Systems Atrophy Case 1 [micrographs - multiframe] Data #2: Unaligned movies of alpha-synuclein filament seeded in vitro by filaments purified from Multiple Systems Atrophy Case 2 [micrographs - multiframe] Data #3: Unaligned movies of alpha-synuclein filament seeded in vitro by filaments purified from Multiple Systems Atrophy Case 5 [micrographs - multiframe] Data #4: Type 1A particles after Bayesian polishing [picked particles - single frame - processed] Data #5: Type 2A particles after Bayesian polishing [picked particles - single frame - processed] Data #6: Type 1B particles after Bayesian polishing [picked particles - single frame - processed] Data #7: Type 2B particles after Bayesian polishing [picked particles - single frame - processed] Data #8: Type 2AB particles after Bayesian polishing [picked particles - single frame - processed] Data #9: Type 3 particles after Bayesian polishing [picked particles - single frame - processed]) |
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Links
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Assembly
Deposited unit | ![]()
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2 | ![]()
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Components
#1: Protein | ![]() Mass: 14476.108 Da / Num. of mol.: 12 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() ![]() |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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EM experiment | Aggregation state: FILAMENT / 3D reconstruction method: helical reconstruction |
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Sample preparation
Component | Name: Alpha synuclein filament / Type: COMPLEX / Entity ID: all / Source: RECOMBINANT |
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Molecular weight | Experimental value: NO |
Source (natural) | Organism: ![]() ![]() |
Source (recombinant) | Organism: ![]() ![]() ![]() ![]() |
Buffer solution | pH: 6.5 |
Specimen | Conc.: 1 mg/ml / Embedding applied: NO / Shadowing applied: NO / Staining applied![]() ![]() |
Vitrification![]() | Instrument: FEI VITROBOT MARK IV / Cryogen name: ETHANE / Humidity: 100 % / Chamber temperature: 277 K / Details: LMB vitrobot IV |
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Electron microscopy imaging
Experimental equipment | ![]() Model: Titan Krios / Image courtesy: FEI Company |
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Microscopy | Model: FEI TITAN KRIOS |
Electron gun | Electron source![]() ![]() |
Electron lens | Mode: BRIGHT FIELD![]() ![]() |
Specimen holder | Cryogen: NITROGEN / Specimen holder model: FEI TITAN KRIOS AUTOGRID HOLDER |
Image recording | Electron dose: 32.6 e/Å2 / Detector mode: COUNTING / Film or detector model: GATAN K2 SUMMIT (4k x 4k) |
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Processing
EM software |
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CTF correction![]() | Type: PHASE FLIPPING AND AMPLITUDE CORRECTION | |||||||||||||||||||||||||
Helical symmerty | Angular rotation/subunit: -0.95 ° / Axial rise/subunit: 4.75 Å / Axial symmetry: C2 | |||||||||||||||||||||||||
3D reconstruction![]() | Resolution: 3.43 Å / Resolution method: FSC 0.143 CUT-OFF / Num. of particles: 82474 / Algorithm: FOURIER SPACE / Symmetry type: HELICAL |