+Open data
-Basic information
Entry | Database: PDB / ID: 7jzy | ||||||
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Title | CryoEM structure of a CRISPR-Cas complex | ||||||
Components |
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Keywords | HYDROLASE/RNA / CRISPR / HYDROLASE-RNA complex | ||||||
Function / homology | Function and homology information | ||||||
Biological species | Pseudomonas aeruginosa (bacteria) | ||||||
Method | ELECTRON MICROSCOPY / single particle reconstruction / cryo EM / Resolution: 3.6 Å | ||||||
Authors | Chang, L. / Li, Z. / Gabel, C. | ||||||
Citation | Journal: To Be Published Title: CryoEM structure of a CRISPR-Cas complex Authors: Chang, L. / Li, Z. / Gabel, C. | ||||||
History |
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-Structure visualization
Movie |
Movie viewer |
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Structure viewer | Molecule: MolmilJmol/JSmol |
-Downloads & links
-Download
PDBx/mmCIF format | 7jzy.cif.gz | 541.4 KB | Display | PDBx/mmCIF format |
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PDB format | pdb7jzy.ent.gz | 443 KB | Display | PDB format |
PDBx/mmJSON format | 7jzy.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 7jzy_validation.pdf.gz | 851.5 KB | Display | wwPDB validaton report |
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Full document | 7jzy_full_validation.pdf.gz | 875.1 KB | Display | |
Data in XML | 7jzy_validation.xml.gz | 77.7 KB | Display | |
Data in CIF | 7jzy_validation.cif.gz | 123.2 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/jz/7jzy ftp://data.pdbj.org/pub/pdb/validation_reports/jz/7jzy | HTTPS FTP |
-Related structure data
Related structure data | 22584MC M: map data used to model this data C: citing same article (ref.) |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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-Components
-Protein , 3 types, 9 molecules ADEFGHIJK
#1: Protein | Mass: 49194.168 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Pseudomonas aeruginosa (bacteria) / Gene: csy1, PA14_33330 / Production host: Escherichia coli BL21(DE3) (bacteria) / References: UniProt: Q02ML9 | ||
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#5: Protein | Mass: 37579.273 Da / Num. of mol.: 6 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Pseudomonas aeruginosa (bacteria) Gene: EQH76_13805, F7O93_18150, NCTC13437_01527, NCTC13619_03982 Production host: Escherichia coli BL21(DE3) (bacteria) / References: UniProt: A0A444M080 #6: Protein | Mass: 7813.900 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Pseudomonas aeruginosa (bacteria) / Production host: Escherichia coli BL21(DE3) (bacteria) |
-Type I-F CRISPR-associated ... , 2 types, 2 molecules BC
#2: Protein | Mass: 36244.074 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Pseudomonas aeruginosa (bacteria) Gene: csy2, ALP65_00953, EQH76_13810, FCG96_17775, PACL_0128 Production host: Escherichia coli BL21(DE3) (bacteria) / References: UniProt: B3G161 |
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#3: Protein | Mass: 21675.781 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Pseudomonas aeruginosa (bacteria) / Gene: cas6f, F7O93_18155 / Production host: Escherichia coli BL21(DE3) (bacteria) / References: UniProt: A0A643HZS6 |
-RNA chain , 1 types, 1 molecules M
#4: RNA chain | Mass: 19538.586 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Pseudomonas aeruginosa (bacteria) / Production host: Escherichia coli BL21(DE3) (bacteria) / References: GenBank: 313291946 |
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-Experimental details
-Experiment
Experiment | Method: ELECTRON MICROSCOPY |
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EM experiment | Aggregation state: PARTICLE / 3D reconstruction method: single particle reconstruction |
-Sample preparation
Component | Name: CRISPR-Cas complex / Type: COMPLEX / Entity ID: all / Source: RECOMBINANT |
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Molecular weight | Experimental value: NO |
Source (natural) | Organism: Pseudomonas aeruginosa (bacteria) |
Source (recombinant) | Organism: Escherichia coli BL21(DE3) (bacteria) |
Buffer solution | pH: 7.5 |
Specimen | Embedding applied: NO / Shadowing applied: NO / Staining applied: NO / Vitrification applied: YES |
Vitrification | Cryogen name: ETHANE |
-Electron microscopy imaging
Experimental equipment | Model: Titan Krios / Image courtesy: FEI Company |
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Microscopy | Model: FEI TITAN KRIOS |
Electron gun | Electron source: FIELD EMISSION GUN / Accelerating voltage: 300 kV / Illumination mode: OTHER |
Electron lens | Mode: BRIGHT FIELD |
Image recording | Electron dose: 54 e/Å2 / Film or detector model: GATAN K3 (6k x 4k) |
-Processing
Software | Name: PHENIX / Version: 1.18.2_3874: / Classification: refinement | ||||||||||||||||||||||||
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CTF correction | Type: PHASE FLIPPING ONLY | ||||||||||||||||||||||||
3D reconstruction | Resolution: 3.6 Å / Resolution method: FSC 0.143 CUT-OFF / Num. of particles: 150697 / Symmetry type: POINT | ||||||||||||||||||||||||
Refine LS restraints |
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